73 Publikationen
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2021 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2957097Shankar, R., Schäffer, N., Schmeer, M., Friehs, K., Risse, J.M., Schleef, M.: Recombinant expression of Barnase in Escherichia coli and its application in plasmid purification. Microbial Cell Factories. 20, : 171 (2021).PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2021 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2958416Luttermann, T., Rückert, C., Wibberg, D., Busche, T., Schwarzhans, J.-P., Friehs, K., Kalinowski, J.: Establishment of a near-contiguous genome sequence of the citric acid producing yeast DSM 3286 with resolution of rDNA clusters and telomeres. NAR Genomics and Bioinformatics. 3, 13 (2021).PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2021 | Patent | PUB-ID: 2965425Risse, J.M., Friehs, K., Wedde, S., Hahn, M., Ludwig, B.: Expression of collagen peptide components in procaryotic systems. 11.11.2021.PUB
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2021 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2957893Shankar, R., Schafer, N., Schmeer, M., Risse, J.M., Friehs, K., Schleef, M.: Correction to: Recombinant expression of Barnase in Escherichia coli and its application in plasmid purifcation. Microbial Cell Factories. 20, : 190 (2021).PUB | DOI | WoS | PubMed | Europe PMC
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2020 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2945772Heß, M., Bloess, S., Risse, J.M., Friehs, K., Fischer von Mollard, G.: Recombinant expression of an l-amino acid oxidase from the fungus Hebeloma cylindrosporum in Pichia pastoris including fermentation. MicrobiologyOpen. 9, : e1112 (2020).PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2020 | Datenpublikation | PUB-ID: 2944932Heß, M., Bloess, S., Risse, J.M., Friehs, K., Fischer von Mollard, G.: Data for 'Recombinant expression of an L-amino acid oxidase from the fungus Hebeloma cylindrosporum in Pichia pastoris including fermentation'. Bielefeld University (2020).PUB | Dateien verfügbar | DOI
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2018 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2930505Kleiner-Grote, G., Risse, J.M., Friehs, K.: Secretion of recombinant proteins from E. coli. ENGINEERING IN LIFE SCIENCES. 18, 532-550 (2018).PUB | DOI | WoS | PubMed | Europe PMC
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2017 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2910668Friehs, K., Schwarzhans, J.P., Luttermann, T., Wibberg, D., Winkler, A., Hübner, W., Huser, T., Kalinowski, J.: A Mitochondrial Autonomously Replicating Sequence from Pichia pastoris for Uniform High Level Recombinant Protein Production. Frontiers in Microbiology. 8, : 780 (2017).PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2017 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2916444Schwarzhans, J.P., Luttermann, T., Geier, M., Kalinowski, J., Friehs, K.: Towards systems metabolic engineering in Pichia pastoris. BIOTECHNOLOGY ADVANCES. 35, 681-710 (2017).PUB | DOI | WoS | PubMed | Europe PMC
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2017 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2908307Perez, F., Risse, J.M., Friehs, K., Wendisch, V.F.: Fermentative production of L-pipecolic acid from glucose and alternative carbon sources. Biotechnology Journal. 12, : 1600646 (2017).PUB | DOI | WoS | PubMed | Europe PMC
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2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2903655Schwarzhans, J.P., Wibberg, D., Winkler, A., Luttermann, T., Kalinowski, J., Friehs, K.: Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector optimization. Microbial Cell Factories. 15, : 84 (2016).PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2907679Schwarzhans, J.P., Wibberg, D., Winkler, A., Luttermann, T., Kalinowski, J., Friehs, K.: Non-canonical integration events in Pichia pastoris encountered during standard transformation analysed with genome sequencing. Scientific Reports. 6, : 38952 (2016).PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2903023Wetzel, D., Müller, J., Flaschel, E., Friehs, K., Risse, J.M.: Fed-batch production and secretion of streptavidin by Hansenula polymorpha: Evaluation of genetic factors and bioprocess development. JOURNAL OF BIOTECHNOLOGY. 225, 3-9 (2016).PUB | DOI | WoS | PubMed | Europe PMC
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2016 | Zeitschriftenaufsatz | E-Veröff. vor dem Druck | PUB-ID: 2906700Holtmann, D., Vernen, F., Müller, J., Kaden, D., Risse, J.M., Friehs, K., Dähne, L., Stratmann, A., Schrader, J.: Effects of particle addition to Streptomyces cultivations to optimize the production of actinorhodin and streptavidin. Sustainable Chemistry and Pharmacy. 5, 67-71 (2016).PUB | DOI | WoS
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2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2906197Müller, J., Bruhn, S., Flaschel, E., Friehs, K., Risse, J.M.: GAP promoter-based fed-batch production of highly bioactive core streptavidin by Pichia pastoris. BIOTECHNOLOGY PROGRESS. 32, 855-864 (2016).PUB | DOI | WoS | PubMed | Europe PMC
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2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2900637Müller, J., Wetzel, D., Flaschel, E., Friehs, K., Risse, J.M.: Constitutive production and efficient secretion of soluble full-length streptavidin by an Escherichia coli 'leaky mutant'. Journal of Biotechnology. 221, 91-100 (2016).PUB | DOI | WoS | PubMed | Europe PMC
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2015 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2759080Grimm, P., Risse, J.M., Müller, J., Cholewa, D., Beshay, U., Friehs, K., Flaschel, E.: Applicability of Euglena gracilis for biorefineries demonstrated by the production of α-tocopherol and paramylon followed by anaerobic digestion. Journal of Biotechnology. 215, 72-79 (2015).PUB | DOI | WoS | PubMed | Europe PMC
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2015 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2722001Schwarzhans, J.P., Cholewa, D., Grimm, P., Beshay, U., Risse, J.M., Friehs, K., Flaschel, E.: Dependency of the fatty acid composition of Euglena gracilis on growth phase and culture conditions. Journal of Applied Phycology. 27, 1389-1399 (2015).PUB | DOI | WoS
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2014 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2679910Selvamani, R.S.V., Telaar, M., Friehs, K., Flaschel, E.: Antibiotic-free segregational plasmid stabilization in Escherichia coli owing to the knockout of triosephosphate isomerase (tpiA). Microbial Cell Factories. 13, : 58 (2014).PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2014 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2675672Selvamani, R.S.V., Friehs, K., Flaschel, E.: Extracellular recombinant protein production under continuous culture conditions with Escherichia coli using an alternative plasmid selection mechanism. Bioprocess and Biosystems Engineering. 37, 401-413 (2014).PUB | DOI | WoS | PubMed | Europe PMC
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2012 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2474324Santek, B., Friehs, K., Lotz, M., Flaschel, E.: Production of paramylon, a ss-1,3-glucan, by heterotrophic growth of Euglena gracilis on potato liquor in fed-batch and repeated-batch mode of cultivation. Engineering in Life Sciences. 12, 89-94 (2012).PUB | DOI | WoS
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2010 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2401962Risse, J.M., Friehs, K.: Sekretion und Affinitätschromatografie rekombinanter Proteine. BIOspektrum. 5, 550-552 (2010).PUB
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2010 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1796536Sommer, B., Friehs, K., Flaschel, E.: Efficient production of extracellular proteins with Escherichia coli by means of optimized coexpression of bacteriocin release proteins. JOURNAL OF BIOTECHNOLOGY. 145, 350-358 (2010).PUB | DOI | WoS | PubMed | Europe PMC
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2009 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1635287Santek, B., Felski, M., Friehs, K., Lotz, M., Flaschel, E.: Production of paramylon, a beta-1,3-glucan, by heterotrophic cultivation of Euglena gracilis on a synthetic medium. ENGINEERING IN LIFE SCIENCES. 9, 23-28 (2009).PUB | DOI | WoS
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2009 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1636091Beshay, U., Miksch, G., Friehs, K., Flaschel, E.: Integrated bioprocess for the production and purification of recombinant proteins by affinity chromatography in Escherichia coli. BIOPROCESS AND BIOSYSTEMS ENGINEERING. 32, 149-158 (2009).PUB | DOI | WoS | PubMed | Europe PMC
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2009 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1634165Sommer, B., Friehs, K., Flaschel, E., Reck, M., Stahl, F., Scheper, T.: Extracellular production and affinity purification of recombinant proteins with Escherichia coli using the versatility of the maltose binding protein. JOURNAL OF BIOTECHNOLOGY. 140, 194-202 (2009).PUB | DOI | WoS | PubMed | Europe PMC
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2008 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1585611Wei, N., Flaschel, E., Friehs, K., Nattkemper, T.W.: A machine vision system for automated non-invasive assessment of cell viability via dark field microscopy, wavelet feature selection and classification. BMC Bioinformatics. 9, 449 (2008).PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2008 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1587590Vijayendran, C., Barsch, A., Friehs, K., Niehaus, K., Becker, A., Flaschel, E.: Perceiving molecular evolution processes in Escherichia coli by comprehensive metabolite and gene expression profiling. GENOME BIOLOGY. 9, R72 (2008).PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1631667Vijayendran, C., Burgemeister, S., Friehs, K., Niehaus, K., Flaschel, E.: 2DBase: 2D-PAGE database of Escherichia coli. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 363, 822-827 (2007).PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1594835Vijayendran, C., Polen, T., Wendisch, V.F., Friehs, K., Niehaus, K., Flaschel, E.: The plasticity of global proteome and genome expression analyzed in closely related W3110 and MG1655 strains of a well-studied model organism, Escherichia coli-K12. Journal of Biotechnology. 128, 747-761 (2007).PUB | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1593120Wei, N., You, J., Friehs, K., Flaschel, E., Nattkemper, T.W.: An in situ probe for on-line monitoring of cell density and viability on the basis of dark field microscopy in conjunction with image processing and supervised machine learning. Biotechnology and Bioengineering. 97, 1489-1500 (2007).PUB | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1595739Wei, N., You, J., Friehs, K., Flaschel, E., Nattkemper, T.W.: In situ dark field microscopy for on-line monitoring of yeast cultures. BIOTECHNOLOGY LETTERS. 29, 373-378 (2007).PUB | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1631107Blaesen, M., Friehs, K., Flaschel, E.: Recycling of bacterial biomass in a process of L-threonine production by means of a recombinant strain of Escherichia coli. JOURNAL OF BIOTECHNOLOGY. 132, 431-437 (2007).PUB | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1631661Beshay, U., Miksch, G., Friehs, K., Flaschel, E.: Increasing the secretion ability of the kil gene for recombinant proteins in Escherichia coli by using a strong stationary-phase promoter. BIOTECHNOLOGY LETTERS. 29, 1893-1901 (2007).PUB | DOI | WoS | PubMed | Europe PMC
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2006 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1600329Miksch, G., Bettenworth, F., Friehs, K., Flaschel, E., Saalbach, A., Nattkemper, T.W.: A rapid reporter system using GFP as a reporter protein for identification and screening of synthetic stationary-phase promoters in Escherichia coli. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 70, 229-236 (2006).PUB | DOI | WoS | PubMed | Europe PMC
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2006 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1597472Blaesen, M., Flaschel, E., Friehs, K.: Sustainable production: Recycling of bacterial biomass resulting from a fermentation process with Klebsiella planticola. CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY. 20, 263-268 (2006).PUB | WoS
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2005 | Kurzbeitrag Konferenz / Poster | PUB-ID: 2906664Polen, T., Wendisch, V.F., Friehs, K., Niehaus, K., Flaschel, E., Vijayendran, C.: The plasticity of global proteome and genome expression analysis in a well studied closely related model organism, Escherichia coli. MOLECULAR & CELLULAR PROTEOMICS. 4, S248-S248 (2005).PUB | WoS
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2005 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1604386Arndt, M., Kleist, S., Miksch, G., Friehs, K., Flaschel, E., Trierweiler, J., Hitzmann, B.: A feedforward-feedback substrate controller based on a Kalman filter for a fed-batch cultivation of Escherichia coli producing phytase. COMPUTERS & CHEMICAL ENGINEERING. 29, 1113-1120 (2005).PUB | DOI | WoS
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2005 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1601074Miksch, G., Bettenworth, F., Friehs, K., Flaschel, E.: The sequence upstream of the -10 consensus sequence modulates the strength and induction time of stationary-phase promoters in Escherichia coli. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 69, 312-320 (2005).PUB | DOI | WoS | PubMed | Europe PMC
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2005 | Konferenzbeitrag | Veröffentlicht | PUB-ID: 1601848Miksch, G., Bettenworth, F., Friehs, K., Flaschel, E., Saalbach, A., Twellmann, T., Nattkemper, T.W.: Libraries of synthetic stationary-phase and stress promoters as a tool for fine-tuning of expression of recombinant proteins in Escherichia coli. Journal of Biotechnology. JOURNAL OF BIOTECHNOLOGY. 120, p. 25-37. ELSEVIER SCIENCE BV (2005).PUB | DOI | WoS | PubMed | Europe PMC
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2005 | Kurzbeitrag Konferenz / Poster | Veröffentlicht | PUB-ID: 1601124Vijayendran, C., Polen, T., Wendisch, V., Friehs, K., Niehaus, K., Flaschel, E.: The plasticity of global proteome and genome expression analysis in a well studied closely related model organism, Escherichia coli. Molecular & Cellular Proteomics. 4, S248 (2005).PUB | WoS
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2004 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1608382Lu, Y.H., Knol, J.C., Linskens, M.H.K., Friehs, K., van Haastert, P.J.M., Flaschel, E.: Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium. JOURNAL OF BIOTECHNOLOGY. 108, 243-251 (2004).PUB | DOI | WoS | PubMed | Europe PMC
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2004 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1609051Voß, C., Schmidt, T., Schleef, M., Friehs, K., Flaschel, E.: Effect of ammonium chloride on plasmid DNA production in high cell density batch culture for biopharmaceutical use. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY. 79, 57-62 (2004).PUB | DOI | WoS
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2004 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1606278Lu, Y.H., Knol, J.C., Linskens, M.H.K., Friehs, K., van Haastert, P.J.M., Flaschel, E.: Cultivation of immobilized Dictyostelium discoideum for the production of soluble human Fas ligand. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 65, 547-552 (2004).PUB | DOI | WoS | PubMed | Europe PMC
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2003 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1609437Beshay, U., Friehs, K., Azzam, A.E.M., Flaschel, E.: Analysis of the behaviour of Dictyostelium discoideum in immobilised state by means of continuous cultivation. BIOPROCESS AND BIOSYSTEMS ENGINEERING. 26, 117-122 (2003).PUB | DOI | WoS | PubMed | Europe PMC
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2003 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1609656Voß, C., Schmidt, T., Schleef, M., Friehs, K., Flaschel, E.: Production of supercoiled multimeric plasmid DNA for biopharmaceutical application. JOURNAL OF BIOTECHNOLOGY. 105, 205-213 (2003).PUB | DOI | WoS | PubMed | Europe PMC
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2002 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1880734Schierl, F., Friehs, K., Risse, J.M., Flaschel, E.: Herstellung von Mediumskomponenten aus mikrobieller Biomasse. Freiberger Forschungshefte. A 866, 112-124 (2002).PUB
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2002 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1613472Miksch, G., Kleist, S., Friehs, K., Flaschel, E.: Overexpression of the phytase from Escherichia coli and its extracellular production in bioreactors. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 59, 685-694 (2002).PUB | DOI | WoS | PubMed | Europe PMC
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2001 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1880242Risse, J.M., Friehs, K., Flaschel, E.: Recycling of Biomass Resulting from Fermentation Processes with Bacillus licheniformis. Chem. Eng. Technol. 24, 141-145 (2001).PUB
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1999 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1622862Miksch, G., Neitzel, R., Friehs, K., Flaschel, E.: High-level expression of a recombinant protein in Klebsiella planticola owing to induced secretion into the culture medium. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 51, 627-632 (1999).PUB | DOI | WoS | PubMed | Europe PMC
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1999 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1621612Schmidt, T., Friehs, K., Schleef, M., Voß, C., Flaschel, E.: Quantitative analysis of plasmid forms by agarose and capillary gel electrophoresis. ANALYTICAL BIOCHEMISTRY. 274, 235-240 (1999).PUB | DOI | WoS | PubMed | Europe PMC
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1997 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1637759Miksch, G., Neitzel, R., Fiedler, E., Friehs, K., Flaschel, E.: Extracellular production of a hybrid beta-glucanase from Bacillus by Escherichia coli under different cultivation conditions in shaking cultures and bioreactors. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 47, 120-126 (1997).PUB | DOI | WoS | PubMed | Europe PMC
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1997 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1627108Poppenborg, L., Friehs, K., Flaschel, E.: The green fluorescent protein is a versatile reporter for bioprocess monitoring. JOURNAL OF BIOTECHNOLOGY. 58, 79-88 (1997).PUB | DOI | WoS | PubMed | Europe PMC
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1996 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1628395Schmidt, T., Friehs, K., Flaschel, E.: Rapid determination of plasmid copy number. JOURNAL OF BIOTECHNOLOGY. 49, 219-229 (1996).PUB | DOI | WoS | PubMed | Europe PMC
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1993 | Sammelwerksbeitrag | Veröffentlicht | PUB-ID: 1782559Reardon, K.F., Friehs, K.: Parameters influencing the productivity of recombinant E. coli cultivations. In: Aarts, R. (ed.) Bioprocess design and control. Advances in biochemical engineering, biotechnology ; 48. p. 53-77. Springer, Berlin [u.a.] (1993).PUB | PDF | PubMed | Europe PMC
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1986 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1782542Friehs, K., Schörgendorfer, K., Schwab, H., Lafferty, R.M.: Cloning and phenotypic expression in Escherichia coli of a Bacillus subtilis gene fragment coding for sucrose hydrolysis. Journal of Biotechnology. 3, 333-341 (1986).PUB | PDF | DOI | WoS