73 Publikationen
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2021 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2957097Shankar, R., Schäffer, N., Schmeer, M., Friehs, K., Risse, J. M., and Schleef, M. (2021). Recombinant expression of Barnase in Escherichia coli and its application in plasmid purification. Microbial Cell Factories 20:171.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2021 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2958416Luttermann, T., Rückert, C., Wibberg, D., Busche, T., Schwarzhans, J. - P., Friehs, K., and Kalinowski, J. (2021). Establishment of a near-contiguous genome sequence of the citric acid producing yeast DSM 3286 with resolution of rDNA clusters and telomeres. NAR Genomics and Bioinformatics 3, 13.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2021 | Patent | PUB-ID: 2965425Risse, J. M., Friehs, K., Wedde, S., Hahn, M., and Ludwig, B. 11.11.2021 Expression of collagen peptide components in procaryotic systems.PUB
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2021 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2957893Shankar, R., Schafer, N., Schmeer, M., Risse, J. M., Friehs, K., and Schleef, M. (2021). Correction to: Recombinant expression of Barnase in Escherichia coli and its application in plasmid purifcation. Microbial Cell Factories 20:190.PUB | DOI | WoS | PubMed | Europe PMC
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2020 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2945772Heß, M., Bloess, S., Risse, J. M., Friehs, K., and Fischer von Mollard, G. (2020). Recombinant expression of an l-amino acid oxidase from the fungus Hebeloma cylindrosporum in Pichia pastoris including fermentation. MicrobiologyOpen 9:e1112.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2020 | Datenpublikation | PUB-ID: 2944932Heß, M., Bloess, S., Risse, J. M., Friehs, K., and Fischer von Mollard, G. (2020). Data for 'Recombinant expression of an L-amino acid oxidase from the fungus Hebeloma cylindrosporum in Pichia pastoris including fermentation'. Bielefeld University.PUB | Dateien verfügbar | DOI
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2018 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2930505Kleiner-Grote, G., Risse, J. M., and Friehs, K. (2018). Secretion of recombinant proteins from E. coli. ENGINEERING IN LIFE SCIENCES 18, 532-550.PUB | DOI | WoS | PubMed | Europe PMC
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2017 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2910668Friehs, K., Schwarzhans, J. P., Luttermann, T., Wibberg, D., Winkler, A., Hübner, W., Huser, T., and Kalinowski, J. (2017). A Mitochondrial Autonomously Replicating Sequence from Pichia pastoris for Uniform High Level Recombinant Protein Production. Frontiers in Microbiology 8:780.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2017 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2916444Schwarzhans, J. P., Luttermann, T., Geier, M., Kalinowski, J., and Friehs, K. (2017). Towards systems metabolic engineering in Pichia pastoris. BIOTECHNOLOGY ADVANCES 35, 681-710.PUB | DOI | WoS | PubMed | Europe PMC
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2017 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2908307Perez, F., Risse, J. M., Friehs, K., and Wendisch, V. F. (2017). Fermentative production of L-pipecolic acid from glucose and alternative carbon sources. Biotechnology Journal 12:1600646.PUB | DOI | WoS | PubMed | Europe PMC
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2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2903655Schwarzhans, J. P., Wibberg, D., Winkler, A., Luttermann, T., Kalinowski, J., and Friehs, K. (2016). Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector optimization. Microbial Cell Factories 15:84.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2907679Schwarzhans, J. P., Wibberg, D., Winkler, A., Luttermann, T., Kalinowski, J., and Friehs, K. (2016). Non-canonical integration events in Pichia pastoris encountered during standard transformation analysed with genome sequencing. Scientific Reports 6:38952.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2903023Wetzel, D., Müller, J., Flaschel, E., Friehs, K., and Risse, J. M. (2016). Fed-batch production and secretion of streptavidin by Hansenula polymorpha: Evaluation of genetic factors and bioprocess development. JOURNAL OF BIOTECHNOLOGY 225, 3-9.PUB | DOI | WoS | PubMed | Europe PMC
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2016 | Zeitschriftenaufsatz | E-Veröff. vor dem Druck | PUB-ID: 2906700Holtmann, D., Vernen, F., Müller, J., Kaden, D., Risse, J. M., Friehs, K., Dähne, L., Stratmann, A., and Schrader, J. (2016). Effects of particle addition to Streptomyces cultivations to optimize the production of actinorhodin and streptavidin. Sustainable Chemistry and Pharmacy 5, 67-71.PUB | DOI | WoS
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2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2906197Müller, J., Bruhn, S., Flaschel, E., Friehs, K., and Risse, J. M. (2016). GAP promoter-based fed-batch production of highly bioactive core streptavidin by Pichia pastoris. BIOTECHNOLOGY PROGRESS 32, 855-864.PUB | DOI | WoS | PubMed | Europe PMC
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2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2900637Müller, J., Wetzel, D., Flaschel, E., Friehs, K., and Risse, J. M. (2016). Constitutive production and efficient secretion of soluble full-length streptavidin by an Escherichia coli 'leaky mutant'. Journal of Biotechnology 221, 91-100.PUB | DOI | WoS | PubMed | Europe PMC
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2015 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2759080Grimm, P., Risse, J. M., Müller, J., Cholewa, D., Beshay, U., Friehs, K., and Flaschel, E. (2015). Applicability of Euglena gracilis for biorefineries demonstrated by the production of α-tocopherol and paramylon followed by anaerobic digestion. Journal of Biotechnology 215, 72-79.PUB | DOI | WoS | PubMed | Europe PMC
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2015 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2722001Schwarzhans, J. P., Cholewa, D., Grimm, P., Beshay, U., Risse, J. M., Friehs, K., and Flaschel, E. (2015). Dependency of the fatty acid composition of Euglena gracilis on growth phase and culture conditions. Journal of Applied Phycology 27, 1389-1399.PUB | DOI | WoS
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2014 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2679910Selvamani, R. S. V., Telaar, M., Friehs, K., and Flaschel, E. (2014). Antibiotic-free segregational plasmid stabilization in Escherichia coli owing to the knockout of triosephosphate isomerase (tpiA). Microbial Cell Factories 13:58.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2014 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2675672Selvamani, R. S. V., Friehs, K., and Flaschel, E. (2014). Extracellular recombinant protein production under continuous culture conditions with Escherichia coli using an alternative plasmid selection mechanism. Bioprocess and Biosystems Engineering 37, 401-413.PUB | DOI | WoS | PubMed | Europe PMC
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2012 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2474324Santek, B., Friehs, K., Lotz, M., and Flaschel, E. (2012). Production of paramylon, a ss-1,3-glucan, by heterotrophic growth of Euglena gracilis on potato liquor in fed-batch and repeated-batch mode of cultivation. Engineering in Life Sciences 12, 89-94.PUB | DOI | WoS
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2010 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2401962Risse, J. M., and Friehs, K. (2010). Sekretion und Affinitätschromatografie rekombinanter Proteine. BIOspektrum 5, 550-552.PUB
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2010 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1796536Sommer, B., Friehs, K., and Flaschel, E. (2010). Efficient production of extracellular proteins with Escherichia coli by means of optimized coexpression of bacteriocin release proteins. JOURNAL OF BIOTECHNOLOGY 145, 350-358.PUB | DOI | WoS | PubMed | Europe PMC
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2009 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1635287Santek, B., Felski, M., Friehs, K., Lotz, M., and Flaschel, E. (2009). Production of paramylon, a beta-1,3-glucan, by heterotrophic cultivation of Euglena gracilis on a synthetic medium. ENGINEERING IN LIFE SCIENCES 9, 23-28.PUB | DOI | WoS
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2009 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1634165Sommer, B., Friehs, K., Flaschel, E., Reck, M., Stahl, F., and Scheper, T. (2009). Extracellular production and affinity purification of recombinant proteins with Escherichia coli using the versatility of the maltose binding protein. JOURNAL OF BIOTECHNOLOGY 140, 194-202.PUB | DOI | WoS | PubMed | Europe PMC
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2009 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1636091Beshay, U., Miksch, G., Friehs, K., and Flaschel, E. (2009). Integrated bioprocess for the production and purification of recombinant proteins by affinity chromatography in Escherichia coli. BIOPROCESS AND BIOSYSTEMS ENGINEERING 32, 149-158.PUB | DOI | WoS | PubMed | Europe PMC
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2008 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1585611Wei, N., Flaschel, E., Friehs, K., and Nattkemper, T. W. (2008). A machine vision system for automated non-invasive assessment of cell viability via dark field microscopy, wavelet feature selection and classification. BMC Bioinformatics 9, 449.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2008 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1587590Vijayendran, C., Barsch, A., Friehs, K., Niehaus, K., Becker, A., and Flaschel, E. (2008). Perceiving molecular evolution processes in Escherichia coli by comprehensive metabolite and gene expression profiling. GENOME BIOLOGY 9, R72.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1631667Vijayendran, C., Burgemeister, S., Friehs, K., Niehaus, K., and Flaschel, E. (2007). 2DBase: 2D-PAGE database of Escherichia coli. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 363, 822-827.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1594835Vijayendran, C., Polen, T., Wendisch, V. F., Friehs, K., Niehaus, K., and Flaschel, E. (2007). The plasticity of global proteome and genome expression analyzed in closely related W3110 and MG1655 strains of a well-studied model organism, Escherichia coli-K12. Journal of Biotechnology 128, 747-761.PUB | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1595739Wei, N., You, J., Friehs, K., Flaschel, E., and Nattkemper, T. W. (2007). In situ dark field microscopy for on-line monitoring of yeast cultures. BIOTECHNOLOGY LETTERS 29, 373-378.PUB | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1593120Wei, N., You, J., Friehs, K., Flaschel, E., and Nattkemper, T. W. (2007). An in situ probe for on-line monitoring of cell density and viability on the basis of dark field microscopy in conjunction with image processing and supervised machine learning. Biotechnology and Bioengineering 97, 1489-1500.PUB | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1631107Blaesen, M., Friehs, K., and Flaschel, E. (2007). Recycling of bacterial biomass in a process of L-threonine production by means of a recombinant strain of Escherichia coli. JOURNAL OF BIOTECHNOLOGY 132, 431-437.PUB | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1631661Beshay, U., Miksch, G., Friehs, K., and Flaschel, E. (2007). Increasing the secretion ability of the kil gene for recombinant proteins in Escherichia coli by using a strong stationary-phase promoter. BIOTECHNOLOGY LETTERS 29, 1893-1901.PUB | DOI | WoS | PubMed | Europe PMC
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2006 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1600329Miksch, G., Bettenworth, F., Friehs, K., Flaschel, E., Saalbach, A., and Nattkemper, T. W. (2006). A rapid reporter system using GFP as a reporter protein for identification and screening of synthetic stationary-phase promoters in Escherichia coli. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 70, 229-236.PUB | DOI | WoS | PubMed | Europe PMC
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2006 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1597472Blaesen, M., Flaschel, E., and Friehs, K. (2006). Sustainable production: Recycling of bacterial biomass resulting from a fermentation process with Klebsiella planticola. CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY 20, 263-268.PUB | WoS
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2005 | Kurzbeitrag Konferenz / Poster | PUB-ID: 2906664Polen, T., Wendisch, V. F., Friehs, K., Niehaus, K., Flaschel, E., and Vijayendran, C. (2005). The plasticity of global proteome and genome expression analysis in a well studied closely related model organism, Escherichia coli. MOLECULAR & CELLULAR PROTEOMICS 4, S248-S248.PUB | WoS
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2005 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1604386Arndt, M., Kleist, S., Miksch, G., Friehs, K., Flaschel, E., Trierweiler, J., and Hitzmann, B. (2005). A feedforward-feedback substrate controller based on a Kalman filter for a fed-batch cultivation of Escherichia coli producing phytase. COMPUTERS & CHEMICAL ENGINEERING 29, 1113-1120.PUB | DOI | WoS
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2005 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1601074Miksch, G., Bettenworth, F., Friehs, K., and Flaschel, E. (2005). The sequence upstream of the -10 consensus sequence modulates the strength and induction time of stationary-phase promoters in Escherichia coli. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 69, 312-320.PUB | DOI | WoS | PubMed | Europe PMC
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2005 | Konferenzbeitrag | Veröffentlicht | PUB-ID: 1601848Miksch, G., Bettenworth, F., Friehs, K., Flaschel, E., Saalbach, A., Twellmann, T., and Nattkemper, T. W. (2005). “Libraries of synthetic stationary-phase and stress promoters as a tool for fine-tuning of expression of recombinant proteins in Escherichia coli” in Journal of Biotechnology JOURNAL OF BIOTECHNOLOGY, vol. 120, (ELSEVIER SCIENCE BV), 25-37.PUB | DOI | WoS | PubMed | Europe PMC
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2005 | Kurzbeitrag Konferenz / Poster | Veröffentlicht | PUB-ID: 1601124Vijayendran, C., Polen, T., Wendisch, V., Friehs, K., Niehaus, K., and Flaschel, E. (2005). The plasticity of global proteome and genome expression analysis in a well studied closely related model organism, Escherichia coli. Molecular & Cellular Proteomics 4, S248.PUB | WoS
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2004 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1608382Lu, Y. H., Knol, J. C., Linskens, M. H. K., Friehs, K., van Haastert, P. J. M., and Flaschel, E. (2004). Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium. JOURNAL OF BIOTECHNOLOGY 108, 243-251.PUB | DOI | WoS | PubMed | Europe PMC
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2004 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1609051Voß, C., Schmidt, T., Schleef, M., Friehs, K., and Flaschel, E. (2004). Effect of ammonium chloride on plasmid DNA production in high cell density batch culture for biopharmaceutical use. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 79, 57-62.PUB | DOI | WoS
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2004 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1606278Lu, Y. H., Knol, J. C., Linskens, M. H. K., Friehs, K., van Haastert, P. J. M., and Flaschel, E. (2004). Cultivation of immobilized Dictyostelium discoideum for the production of soluble human Fas ligand. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 65, 547-552.PUB | DOI | WoS | PubMed | Europe PMC
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2003 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1609437Beshay, U., Friehs, K., Azzam, A. E. M., and Flaschel, E. (2003). Analysis of the behaviour of Dictyostelium discoideum in immobilised state by means of continuous cultivation. BIOPROCESS AND BIOSYSTEMS ENGINEERING 26, 117-122.PUB | DOI | WoS | PubMed | Europe PMC
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2003 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1609656Voß, C., Schmidt, T., Schleef, M., Friehs, K., and Flaschel, E. (2003). Production of supercoiled multimeric plasmid DNA for biopharmaceutical application. JOURNAL OF BIOTECHNOLOGY 105, 205-213.PUB | DOI | WoS | PubMed | Europe PMC
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2002 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1880734Schierl, F., Friehs, K., Risse, J. M., and Flaschel, E. (2002). Herstellung von Mediumskomponenten aus mikrobieller Biomasse. Freiberger Forschungshefte A 866, 112-124.PUB
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2002 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1613472Miksch, G., Kleist, S., Friehs, K., and Flaschel, E. (2002). Overexpression of the phytase from Escherichia coli and its extracellular production in bioreactors. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 59, 685-694.PUB | DOI | WoS | PubMed | Europe PMC
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2001 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1880242Risse, J. M., Friehs, K., and Flaschel, E. (2001). Recycling of Biomass Resulting from Fermentation Processes with Bacillus licheniformis. Chem. Eng. Technol. 24, 141-145.PUB
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1999 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1622862Miksch, G., Neitzel, R., Friehs, K., and Flaschel, E. (1999). High-level expression of a recombinant protein in Klebsiella planticola owing to induced secretion into the culture medium. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 51, 627-632.PUB | DOI | WoS | PubMed | Europe PMC
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1999 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1621612Schmidt, T., Friehs, K., Schleef, M., Voß, C., and Flaschel, E. (1999). Quantitative analysis of plasmid forms by agarose and capillary gel electrophoresis. ANALYTICAL BIOCHEMISTRY 274, 235-240.PUB | DOI | WoS | PubMed | Europe PMC
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1997 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1637759Miksch, G., Neitzel, R., Fiedler, E., Friehs, K., and Flaschel, E. (1997). Extracellular production of a hybrid beta-glucanase from Bacillus by Escherichia coli under different cultivation conditions in shaking cultures and bioreactors. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 47, 120-126.PUB | DOI | WoS | PubMed | Europe PMC
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1997 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1627108Poppenborg, L., Friehs, K., and Flaschel, E. (1997). The green fluorescent protein is a versatile reporter for bioprocess monitoring. JOURNAL OF BIOTECHNOLOGY 58, 79-88.PUB | DOI | WoS | PubMed | Europe PMC
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1996 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1628395Schmidt, T., Friehs, K., and Flaschel, E. (1996). Rapid determination of plasmid copy number. JOURNAL OF BIOTECHNOLOGY 49, 219-229.PUB | DOI | WoS | PubMed | Europe PMC
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1993 | Sammelwerksbeitrag | Veröffentlicht | PUB-ID: 1782559Reardon, K. F., and Friehs, K. (1993). “Parameters influencing the productivity of recombinant E. coli cultivations” in Bioprocess design and control, Aarts, R. ed. Advances in biochemical engineering, biotechnology ; 48 (Berlin [u.a.]: Springer), 53-77.PUB | PDF | PubMed | Europe PMC
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1991 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1782553Kumar, P. K. R., Maschke, H. - E., Friehs, K., and Schügerl, K. (1991). Strategies for improving plasmid stability in genetically modified bacteria in bioreactors. Trends in biotechnology 9, 279-284.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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1986 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1782542Friehs, K., Schörgendorfer, K., Schwab, H., and Lafferty, R. M. (1986). Cloning and phenotypic expression in Escherichia coli of a Bacillus subtilis gene fragment coding for sucrose hydrolysis. Journal of Biotechnology 3, 333-341.PUB | PDF | DOI | WoS