Production of paramylon, a ss-1,3-glucan, by heterotrophic growth of Euglena gracilis on potato liquor in fed-batch and repeated-batch mode of cultivation

Santek B, Friehs K, Lotz M, Flaschel E (2012)
Engineering in Life Sciences 12(1): 89-94.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Santek, Bozidar; Friehs, KarlUniBi; Lotz, Martin; Flaschel, Erwin
Abstract / Bemerkung
Recently, it had been shown that Euglena gracilis was able to grow heterotrophically not only on synthetic media, but also on media based on potato liquor. Supplementation with glucose in both cases led to the accumulation of paramylon, a beta-1,3-glucan. Thus, such a process may yield a valuable product accompanied by the revaluation of an otherwise annoying waste stream of the potato-starch industry. Actually, process strategies have been evaluated in order to optimise the concentration of paramylon obtained at the end of the cultivation process. Therefore, cultivation processes based on fed-batch and in particular repeated-batch strategies have been studied. It is shown that repeated-batch operation maybe particularly suited for such a process since E. gracilis seems to adapt gradually to the cultivation medium so that the concentration of media components may be increased step by step. Repeated-batch cultivation of E. gracilis leads to biomass concentrations in access of 20 g/L with a consistent paramylon mass fraction of about 75%. Cultivations have been carried out at an operating temperature of 27.5 degrees C. As had been found earlier already, pH control is not required during cultivation. On the basis of these results it is clear that repeated-batch cultivation represent a simple and economic way for the production of paramylon by heterotrophic cultivation of E. gracilis.
Stichworte
Fed-batch; Euglena gracilis; ss-1; 3-Glucan; Repeated-batch cultivation; Heterotrophic growth
Erscheinungsjahr
2012
Zeitschriftentitel
Engineering in Life Sciences
Band
12
Ausgabe
1
Seite(n)
89-94
ISSN
1618-0240
Page URI
https://pub.uni-bielefeld.de/record/2474324

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Santek B, Friehs K, Lotz M, Flaschel E. Production of paramylon, a ss-1,3-glucan, by heterotrophic growth of Euglena gracilis on potato liquor in fed-batch and repeated-batch mode of cultivation. Engineering in Life Sciences. 2012;12(1):89-94.
Santek, B., Friehs, K., Lotz, M., & Flaschel, E. (2012). Production of paramylon, a ss-1,3-glucan, by heterotrophic growth of Euglena gracilis on potato liquor in fed-batch and repeated-batch mode of cultivation. Engineering in Life Sciences, 12(1), 89-94. doi:10.1002/elsc.201100025
Santek, Bozidar, Friehs, Karl, Lotz, Martin, and Flaschel, Erwin. 2012. “Production of paramylon, a ss-1,3-glucan, by heterotrophic growth of Euglena gracilis on potato liquor in fed-batch and repeated-batch mode of cultivation”. Engineering in Life Sciences 12 (1): 89-94.
Santek, B., Friehs, K., Lotz, M., and Flaschel, E. (2012). Production of paramylon, a ss-1,3-glucan, by heterotrophic growth of Euglena gracilis on potato liquor in fed-batch and repeated-batch mode of cultivation. Engineering in Life Sciences 12, 89-94.
Santek, B., et al., 2012. Production of paramylon, a ss-1,3-glucan, by heterotrophic growth of Euglena gracilis on potato liquor in fed-batch and repeated-batch mode of cultivation. Engineering in Life Sciences, 12(1), p 89-94.
B. Santek, et al., “Production of paramylon, a ss-1,3-glucan, by heterotrophic growth of Euglena gracilis on potato liquor in fed-batch and repeated-batch mode of cultivation”, Engineering in Life Sciences, vol. 12, 2012, pp. 89-94.
Santek, B., Friehs, K., Lotz, M., Flaschel, E.: Production of paramylon, a ss-1,3-glucan, by heterotrophic growth of Euglena gracilis on potato liquor in fed-batch and repeated-batch mode of cultivation. Engineering in Life Sciences. 12, 89-94 (2012).
Santek, Bozidar, Friehs, Karl, Lotz, Martin, and Flaschel, Erwin. “Production of paramylon, a ss-1,3-glucan, by heterotrophic growth of Euglena gracilis on potato liquor in fed-batch and repeated-batch mode of cultivation”. Engineering in Life Sciences 12.1 (2012): 89-94.
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