Increasing the secretion ability of the kil gene for recombinant proteins in Escherichia coli by using a strong stationary-phase promoter

Beshay U, Miksch G, Friehs K, Flaschel E (2007)
BIOTECHNOLOGY LETTERS 29(12): 1893-1901.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Abstract / Bemerkung
By using a beta-glucanase from Bacillus as a model protein, we investigated whether the secretion competence based on the action of the kil gene can be improved using stronger promoters for the expression of the kil gene. Since the production of extracellular target proteins also depends on the promoter strengths of the target gene, we constructed four expression vectors with all possible combinations of a weak and a strong stationary-phase promoter for the kil gene, and a weak and a strong constitutive promoter, respectively, for the beta-glucanase gene. The results of batch fermentations showed that the use of stronger promoters generally decreased the cell density. However, a drastic increase of productivity of the cells to produce and secrete beta-glucanase resulted in a significantly higher activity of extracellular beta-glucanase. The yield of extracellular beta-glucanase can be increased (to 168 %) by using a strong promoter for the beta-glucanase alone. However, the increase was much higher when the weak promoter of the kil gene was replaced by a strong stationary-phase promoter (to 221 %). An even higher yield of extracellular beta-glucanase was reached when beta-glucanase was expressed by a strong promoter in addition indicating a combinatorial effect. This shows that the extracellular production of a recombinant target gene can be optimized by tuning the promoter strengths of components, the kil gene and the target gene.
Stichworte
E; Fermentation; synthetic stationary phase promoter; coli; beta-glucanase
Erscheinungsjahr
2007
Zeitschriftentitel
BIOTECHNOLOGY LETTERS
Band
29
Ausgabe
12
Seite(n)
1893-1901
ISSN
0141-5492
eISSN
1573-6776
Page URI
https://pub.uni-bielefeld.de/record/1631661

Zitieren

Beshay U, Miksch G, Friehs K, Flaschel E. Increasing the secretion ability of the kil gene for recombinant proteins in Escherichia coli by using a strong stationary-phase promoter. BIOTECHNOLOGY LETTERS. 2007;29(12):1893-1901.
Beshay, U., Miksch, G., Friehs, K., & Flaschel, E. (2007). Increasing the secretion ability of the kil gene for recombinant proteins in Escherichia coli by using a strong stationary-phase promoter. BIOTECHNOLOGY LETTERS, 29(12), 1893-1901. https://doi.org/10.1007/s10529-007-9477-4
Beshay, Usama, Miksch, Gerd, Friehs, Karl, and Flaschel, Erwin. 2007. “Increasing the secretion ability of the kil gene for recombinant proteins in Escherichia coli by using a strong stationary-phase promoter”. BIOTECHNOLOGY LETTERS 29 (12): 1893-1901.
Beshay, U., Miksch, G., Friehs, K., and Flaschel, E. (2007). Increasing the secretion ability of the kil gene for recombinant proteins in Escherichia coli by using a strong stationary-phase promoter. BIOTECHNOLOGY LETTERS 29, 1893-1901.
Beshay, U., et al., 2007. Increasing the secretion ability of the kil gene for recombinant proteins in Escherichia coli by using a strong stationary-phase promoter. BIOTECHNOLOGY LETTERS, 29(12), p 1893-1901.
U. Beshay, et al., “Increasing the secretion ability of the kil gene for recombinant proteins in Escherichia coli by using a strong stationary-phase promoter”, BIOTECHNOLOGY LETTERS, vol. 29, 2007, pp. 1893-1901.
Beshay, U., Miksch, G., Friehs, K., Flaschel, E.: Increasing the secretion ability of the kil gene for recombinant proteins in Escherichia coli by using a strong stationary-phase promoter. BIOTECHNOLOGY LETTERS. 29, 1893-1901 (2007).
Beshay, Usama, Miksch, Gerd, Friehs, Karl, and Flaschel, Erwin. “Increasing the secretion ability of the kil gene for recombinant proteins in Escherichia coli by using a strong stationary-phase promoter”. BIOTECHNOLOGY LETTERS 29.12 (2007): 1893-1901.

8 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Screening for conditions of enhanced production of a recombinant beta-glucanase secreted into the medium by Escherichia coli.
Spexard M, Beshay U, Risse JM, Miksch G, Flaschel E., Biotechnol Lett 32(2), 2010
PMID: 19816658
Extracellular recombinant protein production from Escherichia coli.
Ni Y, Chen R., Biotechnol Lett 31(11), 2009
PMID: 19597765
Construction of a stress-induced system in Escherichia coli for efficient polyhydroxyalkanoates production.
Kang Z, Wang Q, Zhang H, Qi Q., Appl Microbiol Biotechnol 79(2), 2008
PMID: 18347791

19 References

Daten bereitgestellt von Europe PubMed Central.


U, Eng Life Sci 7(), 2007

AUTHOR UNKNOWN, 0

U, Arab J Biotechnol 6(), 2003

U, Proc Biochem 38(), 2003
Protein secretion controlled by a synthetic gene in Escherichia coli.
Blanchin-Roland S, Masson JM., Protein Eng. 2(6), 1989
PMID: 2652141
Hybrid bacillus endo-(1-3,1-4)-beta-glucanases: construction of recombinant genes and molecular properties of the gene products.
Borriss R, Olsen O, Thomsen KK, von Wettstein D., Carlsberg Res. Commun. 54(2), 1989
PMID: 2673278

PR, Appl Env Microbiol 64(), 1998

G, 2001
Overexpression of the phytase from Escherichia coli and its extracellular production in bioreactors.
Miksch G, Kleist S, Friehs K, Flaschel E., Appl. Microbiol. Biotechnol. 59(6), 2002
PMID: 12226725

J, 1989
Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®
Quellen

PMID: 17653622
PubMed | Europe PMC

Suchen in

Google Scholar