Extracellular production and affinity purification of recombinant proteins with Escherichia coli using the versatility of the maltose binding protein

Sommer B, Friehs K, Flaschel E, Reck M, Stahl F, Scheper T (2009)
JOURNAL OF BIOTECHNOLOGY 140(3-4): 194-202.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Recombinant proteins are essential products of today's industrial biotechnology. In this study we address two crucial factors in recombinant protein production: (i) product accessibility and (ii) product recovery. Escherichia coli, one of the most frequently used hosts for recombinant protein expression, does not inherently secrete proteins into the extracellular environment. The major drawback of this expression system is, therefore, to be found in the intracellular protein accumulation and hampered product accessibility. We have constructed a set of expression vectors in order to facilitate extracellular protein production and purification. The maltose binding protein from E call is used as fusion partner for several proteins of interest allowing an export to the bacteria's periplasm via both the Sec and the Tat pathway. Upon coexpression of a modified Cloacin DF13 bacteriocin release protein, the hybrid proteins are released into the culture medium. This essentially applies to a distinguished reporter molecule, the green fluorescent protein, for which an extracellular production was not reported so far. The sequestered proteins can be purified to approximate homogeneity by a simple, rapid and cheap procedure which utilizes the affinity of the maltose binding protein to alpha-1,4-glucans. (C) 2009 Elsevier B.V. All rights reserved.
Stichworte
Escherichia coli; Extracellular production; Secretory expression; Recombinant protein; Affinity purification; Maltose binding protein
Erscheinungsjahr
2009
Zeitschriftentitel
JOURNAL OF BIOTECHNOLOGY
Band
140
Ausgabe
3-4
Seite(n)
194-202
ISSN
0168-1656
Page URI
https://pub.uni-bielefeld.de/record/1634165

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Sommer B, Friehs K, Flaschel E, Reck M, Stahl F, Scheper T. Extracellular production and affinity purification of recombinant proteins with Escherichia coli using the versatility of the maltose binding protein. JOURNAL OF BIOTECHNOLOGY. 2009;140(3-4):194-202.
Sommer, B., Friehs, K., Flaschel, E., Reck, M., Stahl, F., & Scheper, T. (2009). Extracellular production and affinity purification of recombinant proteins with Escherichia coli using the versatility of the maltose binding protein. JOURNAL OF BIOTECHNOLOGY, 140(3-4), 194-202. doi:10.1016/j.jbiotec.2009.01.010
Sommer, B., Friehs, K., Flaschel, E., Reck, M., Stahl, F., and Scheper, T. (2009). Extracellular production and affinity purification of recombinant proteins with Escherichia coli using the versatility of the maltose binding protein. JOURNAL OF BIOTECHNOLOGY 140, 194-202.
Sommer, B., et al., 2009. Extracellular production and affinity purification of recombinant proteins with Escherichia coli using the versatility of the maltose binding protein. JOURNAL OF BIOTECHNOLOGY, 140(3-4), p 194-202.
B. Sommer, et al., “Extracellular production and affinity purification of recombinant proteins with Escherichia coli using the versatility of the maltose binding protein”, JOURNAL OF BIOTECHNOLOGY, vol. 140, 2009, pp. 194-202.
Sommer, B., Friehs, K., Flaschel, E., Reck, M., Stahl, F., Scheper, T.: Extracellular production and affinity purification of recombinant proteins with Escherichia coli using the versatility of the maltose binding protein. JOURNAL OF BIOTECHNOLOGY. 140, 194-202 (2009).
Sommer, Benjamin, Friehs, Karl, Flaschel, Erwin, Reck, Michael, Stahl, Frank, and Scheper, Thomas. “Extracellular production and affinity purification of recombinant proteins with Escherichia coli using the versatility of the maltose binding protein”. JOURNAL OF BIOTECHNOLOGY 140.3-4 (2009): 194-202.

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