73 Publikationen
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2021 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2957097Shankar, R.; Schäffer, N.; Schmeer, M.; Friehs, K.; Risse, J. M.; Schleef, M. (2021): Recombinant expression of Barnase in Escherichia coli and its application in plasmid purification Microbial Cell Factories,20:171PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2021 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2958416Luttermann, T.; Rückert, C.; Wibberg, D.; Busche, T.; Schwarzhans, J. - P.; Friehs, K.; Kalinowski, J. (2021): Establishment of a near-contiguous genome sequence of the citric acid producing yeast DSM 3286 with resolution of rDNA clusters and telomeres NAR Genomics and Bioinformatics,3:(4): 13.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2021 | Patent | PUB-ID: 2965425Risse, J. M.; Friehs, K.; Wedde, S.; Hahn, M.; Ludwig, B. (11.11.2021): Expression of collagen peptide components in procaryotic systemsPUB
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2021 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2957893Shankar, R.; Schafer, N.; Schmeer, M.; Risse, J. M.; Friehs, K.; Schleef, M. (2021): Correction to: Recombinant expression of Barnase in Escherichia coli and its application in plasmid purifcation Microbial Cell Factories,20:(1):190PUB | DOI | WoS | PubMed | Europe PMC
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2020 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2945772Heß, M.; Bloess, S.; Risse, J. M.; Friehs, K.; Fischer von Mollard, G. (2020): Recombinant expression of an l-amino acid oxidase from the fungus Hebeloma cylindrosporum in Pichia pastoris including fermentation. MicrobiologyOpen,9:(10):e1112PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2020 | Datenpublikation | PUB-ID: 2944932Heß, M.; Bloess, S.; Risse, J. M.; Friehs, K.; Fischer von Mollard, G. (2020): Data for 'Recombinant expression of an L-amino acid oxidase from the fungus Hebeloma cylindrosporum in Pichia pastoris including fermentation'. Bielefeld University.PUB | Dateien verfügbar | DOI
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2018 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2930505Kleiner-Grote, G.; Risse, J. M.; Friehs, K. (2018): Secretion of recombinant proteins from E. coli ENGINEERING IN LIFE SCIENCES,18:(8): 532-550.PUB | DOI | WoS | PubMed | Europe PMC
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2017 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2910668Friehs, K.; Schwarzhans, J. P.; Luttermann, T.; Wibberg, D.; Winkler, A.; Hübner, W.; Huser, T.; Kalinowski, J. (2017): A Mitochondrial Autonomously Replicating Sequence from Pichia pastoris for Uniform High Level Recombinant Protein Production Frontiers in Microbiology,8:780PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2017 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2916444Schwarzhans, J. P.; Luttermann, T.; Geier, M.; Kalinowski, J.; Friehs, K. (2017): Towards systems metabolic engineering in Pichia pastoris BIOTECHNOLOGY ADVANCES,35:(6): 681-710.PUB | DOI | WoS | PubMed | Europe PMC
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2017 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2908307Perez, F.; Risse, J. M.; Friehs, K.; Wendisch, V. F. (2017): Fermentative production of L-pipecolic acid from glucose and alternative carbon sources Biotechnology Journal,12:(7):1600646PUB | DOI | WoS | PubMed | Europe PMC
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2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2903655Schwarzhans, J. P.; Wibberg, D.; Winkler, A.; Luttermann, T.; Kalinowski, J.; Friehs, K. (2016): Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector optimization Microbial Cell Factories,15:(1):84PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2907679Schwarzhans, J. P.; Wibberg, D.; Winkler, A.; Luttermann, T.; Kalinowski, J.; Friehs, K. (2016): Non-canonical integration events in Pichia pastoris encountered during standard transformation analysed with genome sequencing Scientific Reports,6:(1):38952PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2903023Wetzel, D.; Müller, J.; Flaschel, E.; Friehs, K.; Risse, J. M. (2016): Fed-batch production and secretion of streptavidin by Hansenula polymorpha: Evaluation of genetic factors and bioprocess development JOURNAL OF BIOTECHNOLOGY,225: 3-9.PUB | DOI | WoS | PubMed | Europe PMC
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2016 | Zeitschriftenaufsatz | E-Veröff. vor dem Druck | PUB-ID: 2906700Holtmann, D.; Vernen, F.; Müller, J.; Kaden, D.; Risse, J. M.; Friehs, K.; Dähne, L.; Stratmann, A.; Schrader, J. (2016): Effects of particle addition to Streptomyces cultivations to optimize the production of actinorhodin and streptavidin Sustainable Chemistry and Pharmacy,5: 67-71.PUB | DOI | WoS
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2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2906197Müller, J.; Bruhn, S.; Flaschel, E.; Friehs, K.; Risse, J. M. (2016): GAP promoter-based fed-batch production of highly bioactive core streptavidin by Pichia pastoris BIOTECHNOLOGY PROGRESS,32:(4): 855-864.PUB | DOI | WoS | PubMed | Europe PMC
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2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2900637Müller, J.; Wetzel, D.; Flaschel, E.; Friehs, K.; Risse, J. M. (2016): Constitutive production and efficient secretion of soluble full-length streptavidin by an Escherichia coli 'leaky mutant' Journal of Biotechnology,221: 91-100.PUB | DOI | WoS | PubMed | Europe PMC
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2015 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2759080Grimm, P.; Risse, J. M.; Müller, J.; Cholewa, D.; Beshay, U.; Friehs, K.; Flaschel, E. (2015): Applicability of Euglena gracilis for biorefineries demonstrated by the production of α-tocopherol and paramylon followed by anaerobic digestion Journal of Biotechnology,215: 72-79.PUB | DOI | WoS | PubMed | Europe PMC
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2015 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2722001Schwarzhans, J. P.; Cholewa, D.; Grimm, P.; Beshay, U.; Risse, J. M.; Friehs, K.; Flaschel, E. (2015): Dependency of the fatty acid composition of Euglena gracilis on growth phase and culture conditions Journal of Applied Phycology,27:(4): 1389-1399.PUB | DOI | WoS
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2014 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2679910Selvamani, R. S. V.; Telaar, M.; Friehs, K.; Flaschel, E. (2014): Antibiotic-free segregational plasmid stabilization in Escherichia coli owing to the knockout of triosephosphate isomerase (tpiA) Microbial Cell Factories,13:(1):58PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2014 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2675672Selvamani, R. S. V.; Friehs, K.; Flaschel, E. (2014): Extracellular recombinant protein production under continuous culture conditions with Escherichia coli using an alternative plasmid selection mechanism Bioprocess and Biosystems Engineering,37:(3): 401-413.PUB | DOI | WoS | PubMed | Europe PMC
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2012 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2474324Santek, B.; Friehs, K.; Lotz, M.; Flaschel, E. (2012): Production of paramylon, a ss-1,3-glucan, by heterotrophic growth of Euglena gracilis on potato liquor in fed-batch and repeated-batch mode of cultivation Engineering in Life Sciences,12:(1): 89-94.PUB | DOI | WoS
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2010 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2401962Risse, J. M.; Friehs, K. (2010): Sekretion und Affinitätschromatografie rekombinanter Proteine BIOspektrum,5: 550-552.PUB
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2010 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1796536Sommer, B.; Friehs, K.; Flaschel, E. (2010): Efficient production of extracellular proteins with Escherichia coli by means of optimized coexpression of bacteriocin release proteins JOURNAL OF BIOTECHNOLOGY,145:(4): 350-358.PUB | DOI | WoS | PubMed | Europe PMC
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2009 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1635287Santek, B.; Felski, M.; Friehs, K.; Lotz, M.; Flaschel, E. (2009): Production of paramylon, a beta-1,3-glucan, by heterotrophic cultivation of Euglena gracilis on a synthetic medium ENGINEERING IN LIFE SCIENCES,9:(1): 23-28.PUB | DOI | WoS
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2009 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1636091Beshay, U.; Miksch, G.; Friehs, K.; Flaschel, E. (2009): Integrated bioprocess for the production and purification of recombinant proteins by affinity chromatography in Escherichia coli BIOPROCESS AND BIOSYSTEMS ENGINEERING,32:(2): 149-158.PUB | DOI | WoS | PubMed | Europe PMC
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2009 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1634165Sommer, B.; Friehs, K.; Flaschel, E.; Reck, M.; Stahl, F.; Scheper, T. (2009): Extracellular production and affinity purification of recombinant proteins with Escherichia coli using the versatility of the maltose binding protein JOURNAL OF BIOTECHNOLOGY,140:(3-4): 194-202.PUB | DOI | WoS | PubMed | Europe PMC
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2008 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1585611Wei, N.; Flaschel, E.; Friehs, K.; Nattkemper, T. W. (2008): A machine vision system for automated non-invasive assessment of cell viability via dark field microscopy, wavelet feature selection and classification BMC Bioinformatics,9:(1): 449.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2008 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1587590Vijayendran, C.; Barsch, A.; Friehs, K.; Niehaus, K.; Becker, A.; Flaschel, E. (2008): Perceiving molecular evolution processes in Escherichia coli by comprehensive metabolite and gene expression profiling GENOME BIOLOGY,9:(4): R72.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1631667Vijayendran, C.; Burgemeister, S.; Friehs, K.; Niehaus, K.; Flaschel, E. (2007): 2DBase: 2D-PAGE database of Escherichia coli BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,363:(3): 822-827.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1594835Vijayendran, C.; Polen, T.; Wendisch, V. F.; Friehs, K.; Niehaus, K.; Flaschel, E. (2007): The plasticity of global proteome and genome expression analyzed in closely related W3110 and MG1655 strains of a well-studied model organism, Escherichia coli-K12 Journal of Biotechnology,128:(4): 747-761.PUB | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1595739Wei, N.; You, J.; Friehs, K.; Flaschel, E.; Nattkemper, T. W. (2007): In situ dark field microscopy for on-line monitoring of yeast cultures BIOTECHNOLOGY LETTERS,29:(3): 373-378.PUB | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1593120Wei, N.; You, J.; Friehs, K.; Flaschel, E.; Nattkemper, T. W. (2007): An in situ probe for on-line monitoring of cell density and viability on the basis of dark field microscopy in conjunction with image processing and supervised machine learning Biotechnology and Bioengineering,97:(6): 1489-1500.PUB | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1631107Blaesen, M.; Friehs, K.; Flaschel, E. (2007): Recycling of bacterial biomass in a process of L-threonine production by means of a recombinant strain of Escherichia coli JOURNAL OF BIOTECHNOLOGY,132:(4): 431-437.PUB | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1631661Beshay, U.; Miksch, G.; Friehs, K.; Flaschel, E. (2007): Increasing the secretion ability of the kil gene for recombinant proteins in Escherichia coli by using a strong stationary-phase promoter BIOTECHNOLOGY LETTERS,29:(12): 1893-1901.PUB | DOI | WoS | PubMed | Europe PMC
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2006 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1600329Miksch, G.; Bettenworth, F.; Friehs, K.; Flaschel, E.; Saalbach, A.; Nattkemper, T. W. (2006): A rapid reporter system using GFP as a reporter protein for identification and screening of synthetic stationary-phase promoters in Escherichia coli APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,70:(2): 229-236.PUB | DOI | WoS | PubMed | Europe PMC
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2006 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1597472Blaesen, M.; Flaschel, E.; Friehs, K. (2006): Sustainable production: Recycling of bacterial biomass resulting from a fermentation process with Klebsiella planticola CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY,20:(3): 263-268.PUB | WoS
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2005 | Kurzbeitrag Konferenz / Poster | PUB-ID: 2906664Polen, T.; Wendisch, V. F.; Friehs, K.; Niehaus, K.; Flaschel, E.; Vijayendran, C. (2005): The plasticity of global proteome and genome expression analysis in a well studied closely related model organism, Escherichia coli MOLECULAR & CELLULAR PROTEOMICS,4:(8): S248-S248.PUB | WoS
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2005 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1604386Arndt, M.; Kleist, S.; Miksch, G.; Friehs, K.; Flaschel, E.; Trierweiler, J.; Hitzmann, B. (2005): A feedforward-feedback substrate controller based on a Kalman filter for a fed-batch cultivation of Escherichia coli producing phytase COMPUTERS & CHEMICAL ENGINEERING,29:(5): 1113-1120.PUB | DOI | WoS
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2005 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1601074Miksch, G.; Bettenworth, F.; Friehs, K.; Flaschel, E. (2005): The sequence upstream of the -10 consensus sequence modulates the strength and induction time of stationary-phase promoters in Escherichia coli APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,69:(3): 312-320.PUB | DOI | WoS | PubMed | Europe PMC
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2005 | Konferenzbeitrag | Veröffentlicht | PUB-ID: 1601848Miksch, G.; Bettenworth, F.; Friehs, K.; Flaschel, E.; Saalbach, A.; Twellmann, T.; Nattkemper, T. W. (2005): Libraries of synthetic stationary-phase and stress promoters as a tool for fine-tuning of expression of recombinant proteins in Escherichia coli. In: Journal of Biotechnology. ELSEVIER SCIENCE BV. (JOURNAL OF BIOTECHNOLOGY, 120). S. 25-37.PUB | DOI | WoS | PubMed | Europe PMC
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2005 | Kurzbeitrag Konferenz / Poster | Veröffentlicht | PUB-ID: 1601124Vijayendran, C.; Polen, T.; Wendisch, V.; Friehs, K.; Niehaus, K.; Flaschel, E. (2005): The plasticity of global proteome and genome expression analysis in a well studied closely related model organism, Escherichia coli Molecular & Cellular Proteomics,4:(8: Suppl. 1): S248.PUB | WoS
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2004 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1609051Voß, C.; Schmidt, T.; Schleef, M.; Friehs, K.; Flaschel, E. (2004): Effect of ammonium chloride on plasmid DNA production in high cell density batch culture for biopharmaceutical use JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,79:(1): 57-62.PUB | DOI | WoS
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2004 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1608382Lu, Y. H.; Knol, J. C.; Linskens, M. H. K.; Friehs, K.; van Haastert, P. J. M.; Flaschel, E. (2004): Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium JOURNAL OF BIOTECHNOLOGY,108:(3): 243-251.PUB | DOI | WoS | PubMed | Europe PMC
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2004 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1606278Lu, Y. H.; Knol, J. C.; Linskens, M. H. K.; Friehs, K.; van Haastert, P. J. M.; Flaschel, E. (2004): Cultivation of immobilized Dictyostelium discoideum for the production of soluble human Fas ligand APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,65:(5): 547-552.PUB | DOI | WoS | PubMed | Europe PMC
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2003 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1609437Beshay, U.; Friehs, K.; Azzam, A. E. M.; Flaschel, E. (2003): Analysis of the behaviour of Dictyostelium discoideum in immobilised state by means of continuous cultivation BIOPROCESS AND BIOSYSTEMS ENGINEERING,26:(2): 117-122.PUB | DOI | WoS | PubMed | Europe PMC
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2003 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1609656Voß, C.; Schmidt, T.; Schleef, M.; Friehs, K.; Flaschel, E. (2003): Production of supercoiled multimeric plasmid DNA for biopharmaceutical application JOURNAL OF BIOTECHNOLOGY,105:(3): 205-213.PUB | DOI | WoS | PubMed | Europe PMC
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2002 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1880734Schierl, F.; Friehs, K.; Risse, J. M.; Flaschel, E. (2002): Herstellung von Mediumskomponenten aus mikrobieller Biomasse Freiberger Forschungshefte,A 866: 112-124.PUB
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2002 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1613472Miksch, G.; Kleist, S.; Friehs, K.; Flaschel, E. (2002): Overexpression of the phytase from Escherichia coli and its extracellular production in bioreactors APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,59:(6): 685-694.PUB | DOI | WoS | PubMed | Europe PMC
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2001 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1880242Risse, J. M.; Friehs, K.; Flaschel, E. (2001): Recycling of Biomass Resulting from Fermentation Processes with Bacillus licheniformis Chem. Eng. Technol.,24:(10): 141-145.PUB
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1999 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1621612Schmidt, T.; Friehs, K.; Schleef, M.; Voß, C.; Flaschel, E. (1999): Quantitative analysis of plasmid forms by agarose and capillary gel electrophoresis ANALYTICAL BIOCHEMISTRY,274:(2): 235-240.PUB | DOI | WoS | PubMed | Europe PMC
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1999 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1622862Miksch, G.; Neitzel, R.; Friehs, K.; Flaschel, E. (1999): High-level expression of a recombinant protein in Klebsiella planticola owing to induced secretion into the culture medium APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,51:(5): 627-632.PUB | DOI | WoS | PubMed | Europe PMC
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1997 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1637759Miksch, G.; Neitzel, R.; Fiedler, E.; Friehs, K.; Flaschel, E. (1997): Extracellular production of a hybrid beta-glucanase from Bacillus by Escherichia coli under different cultivation conditions in shaking cultures and bioreactors APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,47:(2): 120-126.PUB | DOI | WoS | PubMed | Europe PMC
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1997 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1627108Poppenborg, L.; Friehs, K.; Flaschel, E. (1997): The green fluorescent protein is a versatile reporter for bioprocess monitoring JOURNAL OF BIOTECHNOLOGY,58:(2): 79-88.PUB | DOI | WoS | PubMed | Europe PMC
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1996 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1628395Schmidt, T.; Friehs, K.; Flaschel, E. (1996): Rapid determination of plasmid copy number JOURNAL OF BIOTECHNOLOGY,49:(1-3): 219-229.PUB | DOI | WoS | PubMed | Europe PMC
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1993 | Sammelwerksbeitrag | Veröffentlicht | PUB-ID: 1782559Reardon, K. F.; Friehs, K. (1993): Parameters influencing the productivity of recombinant E. coli cultivations. In: R. Aarts (Hrsg.): Bioprocess design and control. Berlin [u.a.]: Springer. (Advances in biochemical engineering, biotechnology ; 48, ). S. 53-77.PUB | PDF | PubMed | Europe PMC
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1991 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1782553Kumar, P. K. R.; Maschke, H. - E.; Friehs, K.; Schügerl, K. (1991): Strategies for improving plasmid stability in genetically modified bacteria in bioreactors Trends in biotechnology,9:(1): 279-284.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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1986 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1782542Friehs, K.; Schörgendorfer, K.; Schwab, H.; Lafferty, R. M. (1986): Cloning and phenotypic expression in Escherichia coli of a Bacillus subtilis gene fragment coding for sucrose hydrolysis Journal of Biotechnology,3:(5-6): 333-341.PUB | PDF | DOI | WoS