Secretion of recombinant proteins from E. coli

Kleiner-Grote G, Risse JM, Friehs K (2018)
ENGINEERING IN LIFE SCIENCES 18(8): 532-550.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
The microorganism Escherichia coli is commonly used for recombinant protein production. Despite several advantageous characteristics like fast growth and high protein yields, its inability to easily secrete recombinant proteins into the extracellular medium remains a drawback for industrial production processes. To overcome this limitation, a multitude of approaches to enhance the extracellular yield and the secretion efficiency of recombinant proteins have been developed in recent years. Here, a comprehensive overview of secretion mechanisms for recombinant proteins from E.coli is given and divided into three main sections. First, the structure of the E. coli cell envelope and the known natural secretion systems are described. Second, the use and optimization of different one- or two-step secretion systems for recombinant protein production, as well as further permeabilization methods are discussed. Finally, the often-overlooked role of cell lysis in secretion studies and its analysis are addressed. So far, effective approaches for increasing the extracellular protein concentration to more than 10 g/L and almost 100% secretion efficiency exist, however, the large range of optimization methods and their combinations suggests that the potential for secretory protein production from E. coli has not yet been fully realized.
Stichworte
Cell lysis; Escherichia coli; Leaky mutants; Recombinant protein; production; Secretion
Erscheinungsjahr
2018
Zeitschriftentitel
ENGINEERING IN LIFE SCIENCES
Band
18
Ausgabe
8
Seite(n)
532-550
ISSN
1618-0240
eISSN
1618-2863
Page URI
https://pub.uni-bielefeld.de/record/2930505

Zitieren

Kleiner-Grote G, Risse JM, Friehs K. Secretion of recombinant proteins from E. coli. ENGINEERING IN LIFE SCIENCES. 2018;18(8):532-550.
Kleiner-Grote, G., Risse, J. M., & Friehs, K. (2018). Secretion of recombinant proteins from E. coli. ENGINEERING IN LIFE SCIENCES, 18(8), 532-550. doi:10.1002/elsc.201700200
Kleiner-Grote, G., Risse, J. M., and Friehs, K. (2018). Secretion of recombinant proteins from E. coli. ENGINEERING IN LIFE SCIENCES 18, 532-550.
Kleiner-Grote, G., Risse, J.M., & Friehs, K., 2018. Secretion of recombinant proteins from E. coli. ENGINEERING IN LIFE SCIENCES, 18(8), p 532-550.
G. Kleiner-Grote, J.M. Risse, and K. Friehs, “Secretion of recombinant proteins from E. coli”, ENGINEERING IN LIFE SCIENCES, vol. 18, 2018, pp. 532-550.
Kleiner-Grote, G., Risse, J.M., Friehs, K.: Secretion of recombinant proteins from E. coli. ENGINEERING IN LIFE SCIENCES. 18, 532-550 (2018).
Kleiner-Grote, Gabriele, Risse, Joe Max, and Friehs, Karl. “Secretion of recombinant proteins from E. coli”. ENGINEERING IN LIFE SCIENCES 18.8 (2018): 532-550.

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