73 Publikationen
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2021 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2957097R. Shankar, N. Schäffer, M. Schmeer, K. Friehs, J. M. Risse, and M. Schleef, “Recombinant expression of Barnase in Escherichia coli and its application in plasmid purification”, Microbial Cell Factories, 2021, 20, : 171.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2021 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2958416T. Luttermann, C. Rückert, D. Wibberg, T. Busche, J. - P. Schwarzhans, K. Friehs, and J. Kalinowski, “Establishment of a near-contiguous genome sequence of the citric acid producing yeast DSM 3286 with resolution of rDNA clusters and telomeres”, NAR Genomics and Bioinformatics, 2021, 3, 13.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2021 | Patent | PUB-ID: 2965425J. M. Risse, K. Friehs, S. Wedde, M. Hahn, and B. Ludwig, “Expression of collagen peptide components in procaryotic systems”, 11.11.2021.PUB
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2021 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2957893R. Shankar, N. Schafer, M. Schmeer, J. M. Risse, K. Friehs, and M. Schleef, “Correction to: Recombinant expression of Barnase in Escherichia coli and its application in plasmid purifcation”, Microbial Cell Factories, 2021, 20, : 190.PUB | DOI | WoS | PubMed | Europe PMC
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2020 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2945772M. Heß, S. Bloess, J. M. Risse, K. Friehs, and G. Fischer von Mollard, “Recombinant expression of an l-amino acid oxidase from the fungus Hebeloma cylindrosporum in Pichia pastoris including fermentation.”, MicrobiologyOpen, 2020, 9, : e1112.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2020 | Datenpublikation | PUB-ID: 2944932M. Heß, S. Bloess, J. M. Risse, K. Friehs, and G. Fischer von Mollard, Data for 'Recombinant expression of an L-amino acid oxidase from the fungus Hebeloma cylindrosporum in Pichia pastoris including fermentation', Bielefeld University, 2020.PUB | Dateien verfügbar | DOI
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2018 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2930505G. Kleiner-Grote, J. M. Risse, and K. Friehs, “Secretion of recombinant proteins from E. coli”, ENGINEERING IN LIFE SCIENCES, 2018, 18, 532-550.PUB | DOI | WoS | PubMed | Europe PMC
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2017 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2910668K. Friehs, J. P. Schwarzhans, T. Luttermann, D. Wibberg, A. Winkler, W. Hübner, T. Huser, and J. Kalinowski, “A Mitochondrial Autonomously Replicating Sequence from Pichia pastoris for Uniform High Level Recombinant Protein Production”, Frontiers in Microbiology, 2017, 8, : 780.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2017 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2916444J. P. Schwarzhans, T. Luttermann, M. Geier, J. Kalinowski, and K. Friehs, “Towards systems metabolic engineering in Pichia pastoris”, BIOTECHNOLOGY ADVANCES, 2017, 35, 681-710.PUB | DOI | WoS | PubMed | Europe PMC
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2017 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2908307F. Perez, J. M. Risse, K. Friehs, and V. F. Wendisch, “Fermentative production of L-pipecolic acid from glucose and alternative carbon sources”, Biotechnology Journal, 2017, 12, : 1600646.PUB | DOI | WoS | PubMed | Europe PMC
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2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2903655J. P. Schwarzhans, D. Wibberg, A. Winkler, T. Luttermann, J. Kalinowski, and K. Friehs, “Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector optimization”, Microbial Cell Factories, 2016, 15, : 84.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2907679J. P. Schwarzhans, D. Wibberg, A. Winkler, T. Luttermann, J. Kalinowski, and K. Friehs, “Non-canonical integration events in Pichia pastoris encountered during standard transformation analysed with genome sequencing”, Scientific Reports, 2016, 6, : 38952.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2903023D. Wetzel, J. Müller, E. Flaschel, K. Friehs, and J. M. Risse, “Fed-batch production and secretion of streptavidin by Hansenula polymorpha: Evaluation of genetic factors and bioprocess development”, JOURNAL OF BIOTECHNOLOGY, 2016, 225, 3-9.PUB | DOI | WoS | PubMed | Europe PMC
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2016 | Zeitschriftenaufsatz | E-Veröff. vor dem Druck | PUB-ID: 2906700D. Holtmann, F. Vernen, J. Müller, D. Kaden, J. M. Risse, K. Friehs, L. Dähne, A. Stratmann, and J. Schrader, “Effects of particle addition to Streptomyces cultivations to optimize the production of actinorhodin and streptavidin”, Sustainable Chemistry and Pharmacy, 2016, 5, 67-71.PUB | DOI | WoS
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2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2906197J. Müller, S. Bruhn, E. Flaschel, K. Friehs, and J. M. Risse, “GAP promoter-based fed-batch production of highly bioactive core streptavidin by Pichia pastoris”, BIOTECHNOLOGY PROGRESS, 2016, 32, 855-864.PUB | DOI | WoS | PubMed | Europe PMC
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2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2900637J. Müller, D. Wetzel, E. Flaschel, K. Friehs, and J. M. Risse, “Constitutive production and efficient secretion of soluble full-length streptavidin by an Escherichia coli 'leaky mutant'”, Journal of Biotechnology, 2016, 221, 91-100.PUB | DOI | WoS | PubMed | Europe PMC
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2015 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2759080P. Grimm, J. M. Risse, J. Müller, D. Cholewa, U. Beshay, K. Friehs, and E. Flaschel, “Applicability of Euglena gracilis for biorefineries demonstrated by the production of α-tocopherol and paramylon followed by anaerobic digestion”, Journal of Biotechnology, 2015, 215, 72-79.PUB | DOI | WoS | PubMed | Europe PMC
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2015 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2722001J. P. Schwarzhans, D. Cholewa, P. Grimm, U. Beshay, J. M. Risse, K. Friehs, and E. Flaschel, “Dependency of the fatty acid composition of Euglena gracilis on growth phase and culture conditions”, Journal of Applied Phycology, 2015, 27, 1389-1399.PUB | DOI | WoS
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2014 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2679910R. S. V. Selvamani, M. Telaar, K. Friehs, and E. Flaschel, “Antibiotic-free segregational plasmid stabilization in Escherichia coli owing to the knockout of triosephosphate isomerase (tpiA)”, Microbial Cell Factories, 2014, 13, : 58.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2014 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2675672R. S. V. Selvamani, K. Friehs, and E. Flaschel, “Extracellular recombinant protein production under continuous culture conditions with Escherichia coli using an alternative plasmid selection mechanism”, Bioprocess and Biosystems Engineering, 2014, 37, 401-413.PUB | DOI | WoS | PubMed | Europe PMC
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2012 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2474324B. Santek, K. Friehs, M. Lotz, and E. Flaschel, “Production of paramylon, a ss-1,3-glucan, by heterotrophic growth of Euglena gracilis on potato liquor in fed-batch and repeated-batch mode of cultivation”, Engineering in Life Sciences, 2012, 12, 89-94.PUB | DOI | WoS
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2010 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2401962J. M. Risse, and K. Friehs, “Sekretion und Affinitätschromatografie rekombinanter Proteine”, BIOspektrum, 2010, 5, 550-552.PUB
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2010 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1796536B. Sommer, K. Friehs, and E. Flaschel, “Efficient production of extracellular proteins with Escherichia coli by means of optimized coexpression of bacteriocin release proteins”, JOURNAL OF BIOTECHNOLOGY, 2010, 145, 350-358.PUB | DOI | WoS | PubMed | Europe PMC
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2009 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1635287B. Santek, M. Felski, K. Friehs, M. Lotz, and E. Flaschel, “Production of paramylon, a beta-1,3-glucan, by heterotrophic cultivation of Euglena gracilis on a synthetic medium”, ENGINEERING IN LIFE SCIENCES, 2009, 9, 23-28.PUB | DOI | WoS
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2009 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1636091U. Beshay, G. Miksch, K. Friehs, and E. Flaschel, “Integrated bioprocess for the production and purification of recombinant proteins by affinity chromatography in Escherichia coli”, BIOPROCESS AND BIOSYSTEMS ENGINEERING, 2009, 32, 149-158.PUB | DOI | WoS | PubMed | Europe PMC
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2009 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1634165B. Sommer, K. Friehs, E. Flaschel, M. Reck, F. Stahl, and T. Scheper, “Extracellular production and affinity purification of recombinant proteins with Escherichia coli using the versatility of the maltose binding protein”, JOURNAL OF BIOTECHNOLOGY, 2009, 140, 194-202.PUB | DOI | WoS | PubMed | Europe PMC
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2008 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1585611N. Wei, E. Flaschel, K. Friehs, and T. W. Nattkemper, “A machine vision system for automated non-invasive assessment of cell viability via dark field microscopy, wavelet feature selection and classification”, BMC Bioinformatics, 2008, 9, 449.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2008 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1587590C. Vijayendran, A. Barsch, K. Friehs, K. Niehaus, A. Becker, and E. Flaschel, “Perceiving molecular evolution processes in Escherichia coli by comprehensive metabolite and gene expression profiling”, GENOME BIOLOGY, 2008, 9, R72.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1631667C. Vijayendran, S. Burgemeister, K. Friehs, K. Niehaus, and E. Flaschel, “2DBase: 2D-PAGE database of Escherichia coli”, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2007, 363, 822-827.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1594835C. Vijayendran, T. Polen, V. F. Wendisch, K. Friehs, K. Niehaus, and E. Flaschel, “The plasticity of global proteome and genome expression analyzed in closely related W3110 and MG1655 strains of a well-studied model organism, Escherichia coli-K12”, Journal of Biotechnology, 2007, 128, 747-761.PUB | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1595739N. Wei, J. You, K. Friehs, E. Flaschel, and T. W. Nattkemper, “In situ dark field microscopy for on-line monitoring of yeast cultures”, BIOTECHNOLOGY LETTERS, 2007, 29, 373-378.PUB | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1593120N. Wei, J. You, K. Friehs, E. Flaschel, and T. W. Nattkemper, “An in situ probe for on-line monitoring of cell density and viability on the basis of dark field microscopy in conjunction with image processing and supervised machine learning”, Biotechnology and Bioengineering, 2007, 97, 1489-1500.PUB | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1631107M. Blaesen, K. Friehs, and E. Flaschel, “Recycling of bacterial biomass in a process of L-threonine production by means of a recombinant strain of Escherichia coli”, JOURNAL OF BIOTECHNOLOGY, 2007, 132, 431-437.PUB | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1631661U. Beshay, G. Miksch, K. Friehs, and E. Flaschel, “Increasing the secretion ability of the kil gene for recombinant proteins in Escherichia coli by using a strong stationary-phase promoter”, BIOTECHNOLOGY LETTERS, 2007, 29, 1893-1901.PUB | DOI | WoS | PubMed | Europe PMC
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2006 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1600329G. Miksch, F. Bettenworth, K. Friehs, E. Flaschel, A. Saalbach, and T. W. Nattkemper, “A rapid reporter system using GFP as a reporter protein for identification and screening of synthetic stationary-phase promoters in Escherichia coli”, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2006, 70, 229-236.PUB | DOI | WoS | PubMed | Europe PMC
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2006 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1597472M. Blaesen, E. Flaschel, and K. Friehs, “Sustainable production: Recycling of bacterial biomass resulting from a fermentation process with Klebsiella planticola”, CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY, 2006, 20, 263-268.PUB | WoS
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2005 | Kurzbeitrag Konferenz / Poster | PUB-ID: 2906664T. Polen, V. F. Wendisch, K. Friehs, K. Niehaus, E. Flaschel, and C. Vijayendran, “The plasticity of global proteome and genome expression analysis in a well studied closely related model organism, Escherichia coli”, MOLECULAR & CELLULAR PROTEOMICS, 2005, 4, S248-S248.PUB | WoS
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2005 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1604386M. Arndt, S. Kleist, G. Miksch, K. Friehs, E. Flaschel, J. Trierweiler, and B. Hitzmann, “A feedforward-feedback substrate controller based on a Kalman filter for a fed-batch cultivation of Escherichia coli producing phytase”, COMPUTERS & CHEMICAL ENGINEERING, 2005, 29, 1113-1120.PUB | DOI | WoS
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2005 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1601074G. Miksch, F. Bettenworth, K. Friehs, and E. Flaschel, “The sequence upstream of the -10 consensus sequence modulates the strength and induction time of stationary-phase promoters in Escherichia coli”, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2005, 69, 312-320.PUB | DOI | WoS | PubMed | Europe PMC
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2005 | Konferenzbeitrag | Veröffentlicht | PUB-ID: 1601848G. Miksch, F. Bettenworth, K. Friehs, E. Flaschel, A. Saalbach, T. Twellmann, and T. W. Nattkemper, in Journal of Biotechnology, Elsevier Science Bv, 2005, p. 25-37.PUB | DOI | WoS | PubMed | Europe PMC
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2005 | Kurzbeitrag Konferenz / Poster | Veröffentlicht | PUB-ID: 1601124C. Vijayendran, T. Polen, V. Wendisch, K. Friehs, K. Niehaus, and E. Flaschel, “The plasticity of global proteome and genome expression analysis in a well studied closely related model organism, Escherichia coli”, Molecular & Cellular Proteomics, 2005, 4, S248.PUB | WoS
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2004 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1609051C. Voß, T. Schmidt, M. Schleef, K. Friehs, and E. Flaschel, “Effect of ammonium chloride on plasmid DNA production in high cell density batch culture for biopharmaceutical use”, JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 2004, 79, 57-62.PUB | DOI | WoS
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2004 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1608382Y. H. Lu, J. C. Knol, M. H. K. Linskens, K. Friehs, P. J. M. van Haastert, and E. Flaschel, “Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium”, JOURNAL OF BIOTECHNOLOGY, 2004, 108, 243-251.PUB | DOI | WoS | PubMed | Europe PMC
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2004 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1606278Y. H. Lu, J. C. Knol, M. H. K. Linskens, K. Friehs, P. J. M. van Haastert, and E. Flaschel, “Cultivation of immobilized Dictyostelium discoideum for the production of soluble human Fas ligand”, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2004, 65, 547-552.PUB | DOI | WoS | PubMed | Europe PMC
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2003 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1609437U. Beshay, K. Friehs, A. E. M. Azzam, and E. Flaschel, “Analysis of the behaviour of Dictyostelium discoideum in immobilised state by means of continuous cultivation”, BIOPROCESS AND BIOSYSTEMS ENGINEERING, 2003, 26, 117-122.PUB | DOI | WoS | PubMed | Europe PMC
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2003 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1609656C. Voß, T. Schmidt, M. Schleef, K. Friehs, and E. Flaschel, “Production of supercoiled multimeric plasmid DNA for biopharmaceutical application”, JOURNAL OF BIOTECHNOLOGY, 2003, 105, 205-213.PUB | DOI | WoS | PubMed | Europe PMC
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2002 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1880734F. Schierl, K. Friehs, J. M. Risse, and E. Flaschel, “Herstellung von Mediumskomponenten aus mikrobieller Biomasse”, Freiberger Forschungshefte, 2002, A 866, 112-124.PUB
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2002 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1613472G. Miksch, S. Kleist, K. Friehs, and E. Flaschel, “Overexpression of the phytase from Escherichia coli and its extracellular production in bioreactors”, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2002, 59, 685-694.PUB | DOI | WoS | PubMed | Europe PMC
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2001 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1880242J. M. Risse, K. Friehs, and E. Flaschel, “Recycling of Biomass Resulting from Fermentation Processes with Bacillus licheniformis”, Chem. Eng. Technol., 2001, 24, 141-145.PUB
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1999 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1621612T. Schmidt, K. Friehs, M. Schleef, C. Voß, and E. Flaschel, “Quantitative analysis of plasmid forms by agarose and capillary gel electrophoresis”, ANALYTICAL BIOCHEMISTRY, 1999, 274, 235-240.PUB | DOI | WoS | PubMed | Europe PMC
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1999 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1622862G. Miksch, R. Neitzel, K. Friehs, and E. Flaschel, “High-level expression of a recombinant protein in Klebsiella planticola owing to induced secretion into the culture medium”, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1999, 51, 627-632.PUB | DOI | WoS | PubMed | Europe PMC
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1997 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1637759G. Miksch, R. Neitzel, E. Fiedler, K. Friehs, and E. Flaschel, “Extracellular production of a hybrid beta-glucanase from Bacillus by Escherichia coli under different cultivation conditions in shaking cultures and bioreactors”, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1997, 47, 120-126.PUB | DOI | WoS | PubMed | Europe PMC
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1997 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1627108L. Poppenborg, K. Friehs, and E. Flaschel, “The green fluorescent protein is a versatile reporter for bioprocess monitoring”, JOURNAL OF BIOTECHNOLOGY, 1997, 58, 79-88.PUB | DOI | WoS | PubMed | Europe PMC
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1996 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1628395T. Schmidt, K. Friehs, and E. Flaschel, “Rapid determination of plasmid copy number”, JOURNAL OF BIOTECHNOLOGY, 1996, 49, 219-229.PUB | DOI | WoS | PubMed | Europe PMC
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1993 | Sammelwerksbeitrag | Veröffentlicht | PUB-ID: 1782559K. F. Reardon, and K. Friehs, in Bioprocess design and control (Ed.: R. Aarts), Springer, Berlin [u.a.], 1993, p. 53-77.PUB | PDF | PubMed | Europe PMC
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1991 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1782553P. K. R. Kumar, H. - E. Maschke, K. Friehs, and K. Schügerl, “Strategies for improving plasmid stability in genetically modified bacteria in bioreactors”, Trends in biotechnology, 1991, 9, 279-284.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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1986 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1782542K. Friehs, K. Schörgendorfer, H. Schwab, and R. M. Lafferty, “Cloning and phenotypic expression in Escherichia coli of a Bacillus subtilis gene fragment coding for sucrose hydrolysis”, Journal of Biotechnology, 1986, 3, 333-341.PUB | PDF | DOI | WoS