The plasticity of global proteome and genome expression analyzed in closely related W3110 and MG1655 strains of a well-studied model organism, Escherichia coli-K12

Vijayendran C, Polen T, Wendisch VF, Friehs K, Niehaus K, Flaschel E (2007)
Journal of Biotechnology 128(4): 747-761.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
Autor*in
Vijayendran, Chandran; Polen, Tino; Wendisch, Volker F.UniBi ; Friehs, KarlUniBi; Niehaus, KarstenUniBi; Flaschel, ErwinUniBi
Einrichtung
Centrum für Biotechnologie > Institut für Genomforschung und Systembiologie
Centrum für Biotechnologie > Institut für Biochemie und Biotechnik
Centrum für Biotechnologie > Graduate Center
Centrum für Biotechnologie > Arbeitsgruppe V. Wendisch
Fakultät für Biologie > Proteom- und Metabolomforschung
Technische Fakultät > AG Fermentationstechnik
Centrum für Biotechnologie > Arbeitsgruppe E. Flaschel
Centrum für Biotechnologie > Arbeitsgruppe K. Niehaus
Fakultät für Biologie > Genetik der Prokaryoten
Abstract / Bemerkung
The use of Escherichia coli as a model organism has provided a great deal of basic information in biomolecular sciences. Examining trait differences among closely related strains of the same species addresses a fundamental biological question: how much diversity is there at the single species level? The main aim of our research was to identify significant differences in the activities of groups of genes between two laboratory strains of an organism closely related in genome structure. We demonstrate that despite strict and controlled growth conditions, there is high plasticity in the global proteome and genome expression in two closely related E. coli K12 sub-strains (W3110 and MG1655), which differ insignificantly in genome structure. The growth patterns of these two sub-strains were very similar in a well-equipped bioreactor, and their genome structures were shown to be almost identical by DNA microarray. However, detailed profiling of protein and gene expression by 2-dimensional a gel electrophoresis and microarray analysis showed many differentially expressed genes and proteins, combinations of which were highly correlated. The differentially regulated genes and proteins belonged to the following functional categories: genes regulated by sigma subunit of RNA polymerase (RpoS), enterobactin-related genes, and genes involved in central metabolism. C Genes involved in central cell metabolism - the glycolysis pathway, the tricarboxylic acid cycle and the glyoxylate bypass - were differentially regulated at both the mRNA and proteome levels. The strains differ significantly in central metabolism and thus in the generation of precursor metabolites and energy. This high plasticity probably represents a universal feature of metabolic activities in closely related species, and has the potential to reveal differences in regulatory networks. We suggest that unless care is taken in the choice of strains for any validating experiment, the results might be misleading. (c) 2007 Elsevier B.V. All rights reserved.
Stichworte
MG1655; genomics; W3 110; proteomics; E. coli
Erscheinungsjahr
2007
Zeitschriftentitel
Journal of Biotechnology
Band
128
Ausgabe
4
Seite(n)
747-761
ISSN
0168-1656
Page URI
https://pub.uni-bielefeld.de/record/1594835

Zitieren

Vijayendran C, Polen T, Wendisch VF, Friehs K, Niehaus K, Flaschel E. The plasticity of global proteome and genome expression analyzed in closely related W3110 and MG1655 strains of a well-studied model organism, Escherichia coli-K12. Journal of Biotechnology. 2007;128(4):747-761.
Vijayendran, C., Polen, T., Wendisch, V. F., Friehs, K., Niehaus, K., & Flaschel, E. (2007). The plasticity of global proteome and genome expression analyzed in closely related W3110 and MG1655 strains of a well-studied model organism, Escherichia coli-K12. Journal of Biotechnology, 128(4), 747-761. https://doi.org/10.1016/j.jbiotec.2006.12.026
Vijayendran, Chandran, Polen, Tino, Wendisch, Volker F., Friehs, Karl, Niehaus, Karsten, and Flaschel, Erwin. 2007. “The plasticity of global proteome and genome expression analyzed in closely related W3110 and MG1655 strains of a well-studied model organism, Escherichia coli-K12”. Journal of Biotechnology 128 (4): 747-761.
Vijayendran, C., Polen, T., Wendisch, V. F., Friehs, K., Niehaus, K., and Flaschel, E. (2007). The plasticity of global proteome and genome expression analyzed in closely related W3110 and MG1655 strains of a well-studied model organism, Escherichia coli-K12. Journal of Biotechnology 128, 747-761.
Vijayendran, C., et al., 2007. The plasticity of global proteome and genome expression analyzed in closely related W3110 and MG1655 strains of a well-studied model organism, Escherichia coli-K12. Journal of Biotechnology, 128(4), p 747-761.
C. Vijayendran, et al., “The plasticity of global proteome and genome expression analyzed in closely related W3110 and MG1655 strains of a well-studied model organism, Escherichia coli-K12”, Journal of Biotechnology, vol. 128, 2007, pp. 747-761.
Vijayendran, C., Polen, T., Wendisch, V.F., Friehs, K., Niehaus, K., Flaschel, E.: The plasticity of global proteome and genome expression analyzed in closely related W3110 and MG1655 strains of a well-studied model organism, Escherichia coli-K12. Journal of Biotechnology. 128, 747-761 (2007).
Vijayendran, Chandran, Polen, Tino, Wendisch, Volker F., Friehs, Karl, Niehaus, Karsten, and Flaschel, Erwin. “The plasticity of global proteome and genome expression analyzed in closely related W3110 and MG1655 strains of a well-studied model organism, Escherichia coli-K12”. Journal of Biotechnology 128.4 (2007): 747-761.

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