Overexpression of the phytase from Escherichia coli and its extracellular production in bioreactors

Miksch G, Kleist S, Friehs K, Flaschel E (2002)
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 59(6): 685-694.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Abstract / Bemerkung
The gene for phytase from Escherichia coli was sequenced and compared with the appA gene. It was found to be a mutant derivative of the appA gene. After fusion with a C-terminal His-tag, phytase was purified by affinity chromatography and the enzymatic properties were analyzed. To develop a system for overexpression and extracellular production of phytase in E. coli, factors affecting the expression and secretion such as promoter type, host strain and selection pressure were analyzed. Using a secretion system based on the controlled expression of the kil gene, the expression of phytase was improved and the enzyme was released into the culture medium at a high level. An effective fermentation strategy based on fed-batch operation was developed.
Erscheinungsjahr
2002
Zeitschriftentitel
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Band
59
Ausgabe
6
Seite(n)
685-694
ISSN
0175-7598
eISSN
1432-0614
Page URI
https://pub.uni-bielefeld.de/record/1613472

Zitieren

Miksch G, Kleist S, Friehs K, Flaschel E. Overexpression of the phytase from Escherichia coli and its extracellular production in bioreactors. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2002;59(6):685-694.
Miksch, G., Kleist, S., Friehs, K., & Flaschel, E. (2002). Overexpression of the phytase from Escherichia coli and its extracellular production in bioreactors. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 59(6), 685-694. https://doi.org/10.1007/s00253-002-1071-z
Miksch, Gerd, Kleist, S, Friehs, Karl, and Flaschel, Erwin. 2002. “Overexpression of the phytase from Escherichia coli and its extracellular production in bioreactors”. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 59 (6): 685-694.
Miksch, G., Kleist, S., Friehs, K., and Flaschel, E. (2002). Overexpression of the phytase from Escherichia coli and its extracellular production in bioreactors. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 59, 685-694.
Miksch, G., et al., 2002. Overexpression of the phytase from Escherichia coli and its extracellular production in bioreactors. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 59(6), p 685-694.
G. Miksch, et al., “Overexpression of the phytase from Escherichia coli and its extracellular production in bioreactors”, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 59, 2002, pp. 685-694.
Miksch, G., Kleist, S., Friehs, K., Flaschel, E.: Overexpression of the phytase from Escherichia coli and its extracellular production in bioreactors. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 59, 685-694 (2002).
Miksch, Gerd, Kleist, S, Friehs, Karl, and Flaschel, Erwin. “Overexpression of the phytase from Escherichia coli and its extracellular production in bioreactors”. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 59.6 (2002): 685-694.

21 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Display of Escherichia coli Phytase on the Surface of Bacillus subtilis Spore Using CotG as an Anchor Protein.
Mingmongkolchai S, Panbangred W., Appl Biochem Biotechnol 187(3), 2019
PMID: 30088242
Production of Phytase Enzyme by a Bioengineered Probiotic for Degrading of Phytate Phosphorus in the Digestive Tract of Poultry.
Pakbaten B, Majidzadeh Heravi R, Kermanshahi H, Sekhavati MH, Javadmanesh A, Mohammadi Ziarat M., Probiotics Antimicrob Proteins 11(2), 2019
PMID: 29680882
Ensifer meliloti overexpressing Escherichia coli phytase gene (appA) improves phosphorus (P) acquisition in maize plants.
Sharma V, Sharma V, Kumar A, Archana G, Kumar GN., Naturwissenschaften 103(9-10), 2016
PMID: 27597170
Isolation and molecular characterization of thermostable phytase from Bacillus subtilis (BSPhyARRMK33).
Reddy CS, Achary VM, Manna M, Singh J, Kaul T, Reddy MK., Appl Biochem Biotechnol 175(6), 2015
PMID: 25588529
Directed evolution of a highly active Yersinia mollaretii phytase.
Shivange AV, Serwe A, Dennig A, Roccatano D, Haefner S, Schwaneberg U., Appl Microbiol Biotechnol 95(2), 2012
PMID: 22159661
A novel thermostable phytase from the fungus Aspergillus aculeatus RCEF 4894: gene cloning and expression in Pichia pastoris
Ma ZY, Pu SC, Jiang JJ, Huang B, Fan MZ, Li ZZ., World J Microbiol Biotechnol 27(3), 2011
PMID: IND44745664
A thermostable phytase from Bacillus sp. MD2: cloning, expression and high-level production in Escherichia coli.
Tran TT, Mamo G, Mattiasson B, Hatti-Kaul R., J Ind Microbiol Biotechnol 37(3), 2010
PMID: 19997958
Recombinant protein secretion in Escherichia coli.
Mergulhão FJ, Summers DK, Monteiro GA., Biotechnol Adv 23(3), 2005
PMID: 15763404
Expression of Escherichia coli AppA2 phytase in four yeast systems.
Lee S, Kim T, Stahl CH, Lei XG., Biotechnol Lett 27(5), 2005
PMID: 15834794
Biotechnological production and applications of phytases.
Haefner S, Knietsch A, Scholten E, Braun J, Lohscheidt M, Zelder O., Appl Microbiol Biotechnol 68(5), 2005
PMID: 16041577
Optimization of the extracellular production of a bacterial phytase with Escherichia coli by using different fed-batch fermentation strategies.
Kleist S, Miksch G, Hitzmann B, Arndt M, Friehs K, Flaschel E., Appl Microbiol Biotechnol 61(5-6), 2003
PMID: 12764560
Displaying and epitope mapping of CD147 on VCSM13 phages: influence of Escherichia coli strains.
Tayapiwatana C, Arooncharus P, Kasinrerk W., J Immunol Methods 281(1-2), 2003
PMID: 14580891

References

Daten bereitgestellt von Europe PubMed Central.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®
Quellen

PMID: 12226725
PubMed | Europe PMC

Suchen in

Google Scholar