In situ dark field microscopy for on-line monitoring of yeast cultures

Wei N, You J, Friehs K, Flaschel E, Nattkemper TW (2007)
BIOTECHNOLOGY LETTERS 29(3): 373-378.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
A new-type in situ probe has been developed to acquire dark field images of yeast in bioreactors. It has been derived from an in situ bright field microscope that is able to measure cell density in bioreactors during fermentation processes. The illumination part of the probe has been replaced with a dark field device, in which an aspheric condenser is used, so that high contrast dark field images can be obtained. The technique of second imaging is implemented to improve the sharpness of the images by means of a relay lens. This new in situ probe is expected to enable the evaluation of the cell viability without staining owing to modern image processing.
Stichworte
aspheric lens; biomass; density; viability; cell; yeast; dark field microscopy; fermentation
Erscheinungsjahr
2007
Zeitschriftentitel
BIOTECHNOLOGY LETTERS
Band
29
Ausgabe
3
Seite(n)
373-378
ISSN
0141-5492
eISSN
1573-6776
Page URI
https://pub.uni-bielefeld.de/record/1595739

Zitieren

Wei N, You J, Friehs K, Flaschel E, Nattkemper TW. In situ dark field microscopy for on-line monitoring of yeast cultures. BIOTECHNOLOGY LETTERS. 2007;29(3):373-378.
Wei, N., You, J., Friehs, K., Flaschel, E., & Nattkemper, T. W. (2007). In situ dark field microscopy for on-line monitoring of yeast cultures. BIOTECHNOLOGY LETTERS, 29(3), 373-378. https://doi.org/10.1007/s10529-006-9245-x
Wei, Ning, You, Jia, Friehs, Karl, Flaschel, Erwin, and Nattkemper, Tim Wilhelm. 2007. “In situ dark field microscopy for on-line monitoring of yeast cultures”. BIOTECHNOLOGY LETTERS 29 (3): 373-378.
Wei, N., You, J., Friehs, K., Flaschel, E., and Nattkemper, T. W. (2007). In situ dark field microscopy for on-line monitoring of yeast cultures. BIOTECHNOLOGY LETTERS 29, 373-378.
Wei, N., et al., 2007. In situ dark field microscopy for on-line monitoring of yeast cultures. BIOTECHNOLOGY LETTERS, 29(3), p 373-378.
N. Wei, et al., “In situ dark field microscopy for on-line monitoring of yeast cultures”, BIOTECHNOLOGY LETTERS, vol. 29, 2007, pp. 373-378.
Wei, N., You, J., Friehs, K., Flaschel, E., Nattkemper, T.W.: In situ dark field microscopy for on-line monitoring of yeast cultures. BIOTECHNOLOGY LETTERS. 29, 373-378 (2007).
Wei, Ning, You, Jia, Friehs, Karl, Flaschel, Erwin, and Nattkemper, Tim Wilhelm. “In situ dark field microscopy for on-line monitoring of yeast cultures”. BIOTECHNOLOGY LETTERS 29.3 (2007): 373-378.

6 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Online monitoring of cell concentration in high cell density Escherichia coli cultivations using in situ Microscopy.
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PMID: 28780163
A review of non-invasive optical-based image analysis systems for continuous bioprocess monitoring.
Höpfner T, Bluma A, Rudolph G, Lindner P, Scheper T., Bioprocess Biosyst Eng 33(2), 2010
PMID: 19396466
In-situ imaging sensors for bioprocess monitoring: state of the art.
Bluma A, Höpfner T, Lindner P, Rehbock C, Beutel S, Riechers D, Hitzmann B, Scheper T., Anal Bioanal Chem 398(6), 2010
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PMID: IND44238360

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