In situ dark field microscopy for on-line monitoring of yeast cultures
Wei N, You J, Friehs K, Flaschel E, Nattkemper TW (2007)
BIOTECHNOLOGY LETTERS 29(3): 373-378.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Einrichtung
Centrum für Biotechnologie > Graduate Center
Technische Fakultät > AG Biodata Mining
Centrum für Biotechnologie > Arbeitsgruppe T. Nattkemper
Centrum für Biotechnologie > Institut für Biochemie und Biotechnik
Centrum für Biotechnologie > Institut für Bioinformatik
Technische Fakultät > AG Fermentationstechnik
Centrum für Biotechnologie > Arbeitsgruppe E. Flaschel
Technische Fakultät > AG Biodata Mining
Centrum für Biotechnologie > Arbeitsgruppe T. Nattkemper
Centrum für Biotechnologie > Institut für Biochemie und Biotechnik
Centrum für Biotechnologie > Institut für Bioinformatik
Technische Fakultät > AG Fermentationstechnik
Centrum für Biotechnologie > Arbeitsgruppe E. Flaschel
Abstract / Bemerkung
A new-type in situ probe has been developed to acquire dark field images of yeast in bioreactors. It has been derived from an in situ bright field microscope that is able to measure cell density in bioreactors during fermentation processes. The illumination part of the probe has been replaced with a dark field device, in which an aspheric condenser is used, so that high contrast dark field images can be obtained. The technique of second imaging is implemented to improve the sharpness of the images by means of a relay lens. This new in situ probe is expected to enable the evaluation of the cell viability without staining owing to modern image processing.
Stichworte
aspheric lens;
biomass;
density;
viability;
cell;
yeast;
dark field microscopy;
fermentation
Erscheinungsjahr
2007
Zeitschriftentitel
BIOTECHNOLOGY LETTERS
Band
29
Ausgabe
3
Seite(n)
373-378
ISSN
0141-5492
eISSN
1573-6776
Page URI
https://pub.uni-bielefeld.de/record/1595739
Zitieren
Wei N, You J, Friehs K, Flaschel E, Nattkemper TW. In situ dark field microscopy for on-line monitoring of yeast cultures. BIOTECHNOLOGY LETTERS. 2007;29(3):373-378.
Wei, N., You, J., Friehs, K., Flaschel, E., & Nattkemper, T. W. (2007). In situ dark field microscopy for on-line monitoring of yeast cultures. BIOTECHNOLOGY LETTERS, 29(3), 373-378. https://doi.org/10.1007/s10529-006-9245-x
Wei, Ning, You, Jia, Friehs, Karl, Flaschel, Erwin, and Nattkemper, Tim Wilhelm. 2007. “In situ dark field microscopy for on-line monitoring of yeast cultures”. BIOTECHNOLOGY LETTERS 29 (3): 373-378.
Wei, N., You, J., Friehs, K., Flaschel, E., and Nattkemper, T. W. (2007). In situ dark field microscopy for on-line monitoring of yeast cultures. BIOTECHNOLOGY LETTERS 29, 373-378.
Wei, N., et al., 2007. In situ dark field microscopy for on-line monitoring of yeast cultures. BIOTECHNOLOGY LETTERS, 29(3), p 373-378.
N. Wei, et al., “In situ dark field microscopy for on-line monitoring of yeast cultures”, BIOTECHNOLOGY LETTERS, vol. 29, 2007, pp. 373-378.
Wei, N., You, J., Friehs, K., Flaschel, E., Nattkemper, T.W.: In situ dark field microscopy for on-line monitoring of yeast cultures. BIOTECHNOLOGY LETTERS. 29, 373-378 (2007).
Wei, Ning, You, Jia, Friehs, Karl, Flaschel, Erwin, and Nattkemper, Tim Wilhelm. “In situ dark field microscopy for on-line monitoring of yeast cultures”. BIOTECHNOLOGY LETTERS 29.3 (2007): 373-378.
Daten bereitgestellt von European Bioinformatics Institute (EBI)
6 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
Online monitoring of cell concentration in high cell density Escherichia coli cultivations using in situ Microscopy.
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Marquard D, Schneider-Barthold C, Düsterloh S, Scheper T, Lindner P., J Biotechnol 259(), 2017
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An Innovative Optical Sensor for the Online Monitoring and Control of Biomass Concentration in a Membrane Bioreactor System for Lactic Acid Production.
Fan R, Ebrahimi M, Quitmann H, Aden M, Czermak P., Sensors (Basel) 16(3), 2016
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Fan R, Ebrahimi M, Quitmann H, Aden M, Czermak P., Sensors (Basel) 16(3), 2016
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A review of non-invasive optical-based image analysis systems for continuous bioprocess monitoring.
Höpfner T, Bluma A, Rudolph G, Lindner P, Scheper T., Bioprocess Biosyst Eng 33(2), 2010
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Höpfner T, Bluma A, Rudolph G, Lindner P, Scheper T., Bioprocess Biosyst Eng 33(2), 2010
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In-situ imaging sensors for bioprocess monitoring: state of the art.
Bluma A, Höpfner T, Lindner P, Rehbock C, Beutel S, Riechers D, Hitzmann B, Scheper T., Anal Bioanal Chem 398(6), 2010
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Bluma A, Höpfner T, Lindner P, Rehbock C, Beutel S, Riechers D, Hitzmann B, Scheper T., Anal Bioanal Chem 398(6), 2010
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A non-destructive digital imaging method to predict immobilized yeast-biomass
Acevedo CristianA, Skurtys Olivier, Young ManuelE, Enrione Javier, Pedreschi Franco, Osorio Fernando., Lebenson Wiss Technol 42(8), 2009
PMID: IND44238360
Acevedo CristianA, Skurtys Olivier, Young ManuelE, Enrione Javier, Pedreschi Franco, Osorio Fernando., Lebenson Wiss Technol 42(8), 2009
PMID: IND44238360
A machine vision system for automated non-invasive assessment of cell viability via dark field microscopy, wavelet feature selection and classification.
Wei N, Flaschel E, Friehs K, Nattkemper TW., BMC Bioinformatics 9(), 2008
PMID: 18939996
Wei N, Flaschel E, Friehs K, Nattkemper TW., BMC Bioinformatics 9(), 2008
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