In situ dark field microscopy for on-line monitoring of yeast cultures
Wei N, You J, Friehs K, Flaschel E, Nattkemper TW (2007)
BIOTECHNOLOGY LETTERS 29(3): 373-378.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Autor*in
Einrichtung
Centrum für Biotechnologie > Arbeitsgruppe E. Flaschel
Centrum für Biotechnologie > Institut für Bioinformatik
Technische Fakultät > AG Fermentationstechnik
Technische Fakultät > AG Biodata Mining
Centrum für Biotechnologie > Institut für Biochemie und Biotechnik
Centrum für Biotechnologie > Arbeitsgruppe T. Nattkemper
Centrum für Biotechnologie > Graduate Center
Centrum für Biotechnologie > Institut für Bioinformatik
Technische Fakultät > AG Fermentationstechnik
Technische Fakultät > AG Biodata Mining
Centrum für Biotechnologie > Institut für Biochemie und Biotechnik
Centrum für Biotechnologie > Arbeitsgruppe T. Nattkemper
Centrum für Biotechnologie > Graduate Center
Abstract / Bemerkung
A new-type in situ probe has been developed to acquire dark field images of yeast in bioreactors. It has been derived from an in situ bright field microscope that is able to measure cell density in bioreactors during fermentation processes. The illumination part of the probe has been replaced with a dark field device, in which an aspheric condenser is used, so that high contrast dark field images can be obtained. The technique of second imaging is implemented to improve the sharpness of the images by means of a relay lens. This new in situ probe is expected to enable the evaluation of the cell viability without staining owing to modern image processing.
Stichworte
aspheric lens;
biomass;
density;
viability;
cell;
yeast;
dark field microscopy;
fermentation
Erscheinungsjahr
2007
Zeitschriftentitel
BIOTECHNOLOGY LETTERS
Band
29
Ausgabe
3
Seite(n)
373-378
ISSN
0141-5492
eISSN
1573-6776
Page URI
https://pub.uni-bielefeld.de/record/1595739
Zitieren
Wei N, You J, Friehs K, Flaschel E, Nattkemper TW. In situ dark field microscopy for on-line monitoring of yeast cultures. BIOTECHNOLOGY LETTERS. 2007;29(3):373-378.
Wei, N., You, J., Friehs, K., Flaschel, E., & Nattkemper, T. W. (2007). In situ dark field microscopy for on-line monitoring of yeast cultures. BIOTECHNOLOGY LETTERS, 29(3), 373-378. https://doi.org/10.1007/s10529-006-9245-x
Wei, Ning, You, Jia, Friehs, Karl, Flaschel, Erwin, and Nattkemper, Tim Wilhelm. 2007. “In situ dark field microscopy for on-line monitoring of yeast cultures”. BIOTECHNOLOGY LETTERS 29 (3): 373-378.
Wei, N., You, J., Friehs, K., Flaschel, E., and Nattkemper, T. W. (2007). In situ dark field microscopy for on-line monitoring of yeast cultures. BIOTECHNOLOGY LETTERS 29, 373-378.
Wei, N., et al., 2007. In situ dark field microscopy for on-line monitoring of yeast cultures. BIOTECHNOLOGY LETTERS, 29(3), p 373-378.
N. Wei, et al., “In situ dark field microscopy for on-line monitoring of yeast cultures”, BIOTECHNOLOGY LETTERS, vol. 29, 2007, pp. 373-378.
Wei, N., You, J., Friehs, K., Flaschel, E., Nattkemper, T.W.: In situ dark field microscopy for on-line monitoring of yeast cultures. BIOTECHNOLOGY LETTERS. 29, 373-378 (2007).
Wei, Ning, You, Jia, Friehs, Karl, Flaschel, Erwin, and Nattkemper, Tim Wilhelm. “In situ dark field microscopy for on-line monitoring of yeast cultures”. BIOTECHNOLOGY LETTERS 29.3 (2007): 373-378.
6 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
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PMID: 27007380
Fan R, Ebrahimi M, Quitmann H, Aden M, Czermak P., Sensors (Basel) 16(3), 2016
PMID: 27007380
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Höpfner T, Bluma A, Rudolph G, Lindner P, Scheper T., Bioprocess Biosyst Eng 33(2), 2010
PMID: 19396466
Höpfner T, Bluma A, Rudolph G, Lindner P, Scheper T., Bioprocess Biosyst Eng 33(2), 2010
PMID: 19396466
In-situ imaging sensors for bioprocess monitoring: state of the art.
Bluma A, Höpfner T, Lindner P, Rehbock C, Beutel S, Riechers D, Hitzmann B, Scheper T., Anal Bioanal Chem 398(6), 2010
PMID: 20835863
Bluma A, Höpfner T, Lindner P, Rehbock C, Beutel S, Riechers D, Hitzmann B, Scheper T., Anal Bioanal Chem 398(6), 2010
PMID: 20835863
A non-destructive digital imaging method to predict immobilized yeast-biomass
Acevedo CristianA, Skurtys Olivier, Young ManuelE, Enrione Javier, Pedreschi Franco, Osorio Fernando., Lebenson Wiss Technol 42(8), 2009
PMID: IND44238360
Acevedo CristianA, Skurtys Olivier, Young ManuelE, Enrione Javier, Pedreschi Franco, Osorio Fernando., Lebenson Wiss Technol 42(8), 2009
PMID: IND44238360
A machine vision system for automated non-invasive assessment of cell viability via dark field microscopy, wavelet feature selection and classification.
Wei N, Flaschel E, Friehs K, Nattkemper TW., BMC Bioinformatics 9(), 2008
PMID: 18939996
Wei N, Flaschel E, Friehs K, Nattkemper TW., BMC Bioinformatics 9(), 2008
PMID: 18939996
16 References
Daten bereitgestellt von Europe PubMed Central.
C, Biotech Bioeng 60(), 1998
AUTHOR UNKNOWN, 0
JE, Bioproc Biosyst Eng 21(), 1999
H, Tech q - Master Brew Assoc Am 37(), 2000
A computer-aided measuring system for the characterization of yeast populations combining 2D-image analysis, electronic particle counter, and flow cytometry.
Huls PG, Nanninga N, van Spronsen EA, Valkenburg JA, Vishcer NO, Woldringh CL., Biotechnol. Bioeng. 39(3), 1992
PMID: 18600951
Huls PG, Nanninga N, van Spronsen EA, Valkenburg JA, Vishcer NO, Woldringh CL., Biotechnol. Bioeng. 39(3), 1992
PMID: 18600951
In-situ microscopy: Online process monitoring of mammalian cell cultures.
Joeris K, Frerichs JG, Konstantinov K, Scheper T., Cytotechnology 38(1-3), 2002
PMID: 19003094
Joeris K, Frerichs JG, Konstantinov K, Scheper T., Cytotechnology 38(1-3), 2002
PMID: 19003094
TW, IEEE T Inf Technol B 5(), 2001
Human vs machine: evaluation of fluorescence micrographs.
Nattkemper TW, Twellmann T, Ritter H, Schubert W., Comput. Biol. Med. 33(1), 2003
PMID: 12485628
Nattkemper TW, Twellmann T, Ritter H, Schubert W., Comput. Biol. Med. 33(1), 2003
PMID: 12485628
Morphological characterization of yeast by image analysis.
Pons MN, Vivier H, Remy JF, Dodds JA., Biotechnol. Bioeng. 42(11), 1993
PMID: 18612963
Pons MN, Vivier H, Remy JF, Dodds JA., Biotechnol. Bioeng. 42(11), 1993
PMID: 18612963
T, 1991
Biomass determination.
Sonnleitner B, Locher G, Fiechter A., J. Biotechnol. 25(1-2), 1992
PMID: 1368462
Sonnleitner B, Locher G, Fiechter A., J. Biotechnol. 25(1-2), 1992
PMID: 1368462
H, Biotech Bioeng 47(), 1995
Applications of image analysis in cell technology.
Thomas CR, Paul GC., Curr. Opin. Biotechnol. 7(1), 1996
PMID: 8791309
Thomas CR, Paul GC., Curr. Opin. Biotechnol. 7(1), 1996
PMID: 8791309
J, 1998
Biotechnological applications of image analysis: present and future prospects.
Vecht-Lifshitz SE, Ison AP., J. Biotechnol. 23(1), 1992
PMID: 1367947
Vecht-Lifshitz SE, Ison AP., J. Biotechnol. 23(1), 1992
PMID: 1367947
AUTHOR UNKNOWN, 0
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