Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium

Lu YH, Knol JC, Linskens MHK, Friehs K, van Haastert PJM, Flaschel E (2004)
JOURNAL OF BIOTECHNOLOGY 108(3): 243-251.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Human Fas ligand (hFasL) is of considerable interest since it is a type II transmembrane glycoprotein that induces programmed cell death, or apoptosis. In this study Dictyostelium discoideum was used to produce a soluble form of the human Fas ligand. The recombinant cells were adapted to a modified synthetic FM medium, called SIH medium. Cells adapted to the SIH medium reached about 2 times higher cell densities and hFasL concentrations on this medium compared with cells growing on the standard complex medium HL-5C. Even higher values were achieved by a dissolved oxygen-controlled fed-batch cultivation in a conventional stirred bioreactor on SIH medium. Cell densities of up to 5.5 x 10(7) ml(-1) and a maximum hFasL concentration of 148 mug l (1) were obtained. These results were further improved by means of continuous cultivation of D. discoideum in a bioreactor equipped with cell retention by microfiltration. At low space velocity very high cell densities of up to 2.4 x 10(8) ml(-1) and hFasL concentrations of up to 205 mug l(-1) were achieved. (C) 2004 Elsevier B.V. All rights reserved.
Stichworte
fed-batch cultivation; medium; synthetic SIH; Dicryostelium discoideum; human soluble Fas ligand; continuous cultivation; microfiltration
Erscheinungsjahr
2004
Zeitschriftentitel
JOURNAL OF BIOTECHNOLOGY
Band
108
Ausgabe
3
Seite(n)
243-251
ISSN
0
Page URI
https://pub.uni-bielefeld.de/record/1608382

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Lu YH, Knol JC, Linskens MHK, Friehs K, van Haastert PJM, Flaschel E. Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium. JOURNAL OF BIOTECHNOLOGY. 2004;108(3):243-251.
Lu, Y. H., Knol, J. C., Linskens, M. H. K., Friehs, K., van Haastert, P. J. M., & Flaschel, E. (2004). Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium. JOURNAL OF BIOTECHNOLOGY, 108(3), 243-251. doi:10.1016/j.biotec.2003.12.006
Lu, Y. H., Knol, J. C., Linskens, M. H. K., Friehs, K., van Haastert, P. J. M., and Flaschel, E. (2004). Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium. JOURNAL OF BIOTECHNOLOGY 108, 243-251.
Lu, Y.H., et al., 2004. Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium. JOURNAL OF BIOTECHNOLOGY, 108(3), p 243-251.
Y.H. Lu, et al., “Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium”, JOURNAL OF BIOTECHNOLOGY, vol. 108, 2004, pp. 243-251.
Lu, Y.H., Knol, J.C., Linskens, M.H.K., Friehs, K., van Haastert, P.J.M., Flaschel, E.: Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium. JOURNAL OF BIOTECHNOLOGY. 108, 243-251 (2004).
Lu, YH, Knol, JC, Linskens, MHK, Friehs, Karl, van Haastert, PJM, and Flaschel, Erwin. “Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium”. JOURNAL OF BIOTECHNOLOGY 108.3 (2004): 243-251.

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