A rapid reporter system using GFP as a reporter protein for identification and screening of synthetic stationary-phase promoters in Escherichia coli

Miksch G, Bettenworth F, Friehs K, Flaschel E, Saalbach A, Nattkemper TW (2006)
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 70(2): 229-236.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
To develop a rapid reporter system for the screening of stationary-phase promoters in Escherichia coli, the expression pattern of the green fluorescent protein (GFP) during bacterial cultivation was compared with that of the commonly used beta-galactosidase. Using GFP with enhanced fluorescence, the expression pattern of both reporter systems GFP and beta-galactosidase were similar and showed a typical induction of gene activity of the reporter genes, i.e. increase of expression at the transition from exponential to stationary phase. The expression was affected by the culture medium, i.e. in contrast to the complex medium (LB medium), the stationary-phase specific induction was only observed in synthetic medium (M9) when amino acids were added, whereas there was generally no induction in MOPS medium. To develop a rapid screening method on agar plates for stationary-phase promoters, a photographic approach was used, continued with computational image treatment. A screening method is presented which enables an on-line monitoring of gene activity.
Erscheinungsjahr
Zeitschriftentitel
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Band
70
Ausgabe
2
Seite(n)
229-236
ISSN
eISSN
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Miksch G, Bettenworth F, Friehs K, Flaschel E, Saalbach A, Nattkemper TW. A rapid reporter system using GFP as a reporter protein for identification and screening of synthetic stationary-phase promoters in Escherichia coli. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2006;70(2):229-236.
Miksch, G., Bettenworth, F., Friehs, K., Flaschel, E., Saalbach, A., & Nattkemper, T. W. (2006). A rapid reporter system using GFP as a reporter protein for identification and screening of synthetic stationary-phase promoters in Escherichia coli. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 70(2), 229-236. doi:10.1007/s00253-005-0060-4
Miksch, G., Bettenworth, F., Friehs, K., Flaschel, E., Saalbach, A., and Nattkemper, T. W. (2006). A rapid reporter system using GFP as a reporter protein for identification and screening of synthetic stationary-phase promoters in Escherichia coli. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 70, 229-236.
Miksch, G., et al., 2006. A rapid reporter system using GFP as a reporter protein for identification and screening of synthetic stationary-phase promoters in Escherichia coli. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 70(2), p 229-236.
G. Miksch, et al., “A rapid reporter system using GFP as a reporter protein for identification and screening of synthetic stationary-phase promoters in Escherichia coli”, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 70, 2006, pp. 229-236.
Miksch, G., Bettenworth, F., Friehs, K., Flaschel, E., Saalbach, A., Nattkemper, T.W.: A rapid reporter system using GFP as a reporter protein for identification and screening of synthetic stationary-phase promoters in Escherichia coli. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 70, 229-236 (2006).
Miksch, Gerd, Bettenworth, F, Friehs, Karl, Flaschel, Erwin, Saalbach, A, and Nattkemper, Tim Wilhelm. “A rapid reporter system using GFP as a reporter protein for identification and screening of synthetic stationary-phase promoters in Escherichia coli”. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 70.2 (2006): 229-236.

5 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

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Promoter analysis and transcription regulation of fus gene cluster responsible for fusaricidin synthesis of Paenibacillus polymyxa SQR-21.
Li S, Zhang R, Wang Y, Zhang N, Shao J, Qiu M, Shen B, Yin X, Shen Q., Appl Microbiol Biotechnol 97(21), 2013
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Lissemore JL, Bayes J, Calvey M, Reineke L, Colagiavanni A, Tscheiner M, Mascotti DP., Mol Biol Rep 36(5), 2009
PMID: 18622760

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