73 Publikationen
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2021 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2957097Shankar R, Schäffer N, Schmeer M, Friehs K, Risse JM, Schleef M (2021)PUB | PDF | DOI | WoS | PubMed | Europe PMC
Recombinant expression of Barnase in Escherichia coli and its application in plasmid purification.
Microbial Cell Factories 20: 171. -
2021 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2958416Luttermann T, Rückert C, Wibberg D, Busche T, Schwarzhans J-P, Friehs K, Kalinowski J (2021)PUB | PDF | DOI | WoS | PubMed | Europe PMC
Establishment of a near-contiguous genome sequence of the citric acid producing yeast DSM 3286 with resolution of rDNA clusters and telomeres.
NAR Genomics and Bioinformatics 3(4): 13. -
2021 | Patent | PUB-ID: 2965425Risse JM, Friehs K, Wedde S, Hahn M, Ludwig B (11.11.2021)PUB
Expression of collagen peptide components in procaryotic systems. -
2021 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2957893Shankar R, Schafer N, Schmeer M, Risse JM, Friehs K, Schleef M (2021)PUB | DOI | WoS | PubMed | Europe PMC
Correction to: Recombinant expression of Barnase in Escherichia coli and its application in plasmid purifcation.
Microbial Cell Factories 20(1): 190. -
2020 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2945772Heß M, Bloess S, Risse JM, Friehs K, Fischer von Mollard G (2020)PUB | PDF | DOI | WoS | PubMed | Europe PMC
Recombinant expression of an l-amino acid oxidase from the fungus Hebeloma cylindrosporum in Pichia pastoris including fermentation.
MicrobiologyOpen 9(10): e1112. -
2020 | Datenpublikation | PUB-ID: 2944932Heß M, Bloess S, Risse JM, Friehs K, Fischer von Mollard G (2020)PUB | Dateien verfügbar | DOI
Data for 'Recombinant expression of an L-amino acid oxidase from the fungus Hebeloma cylindrosporum in Pichia pastoris including fermentation'.
Bielefeld University. -
2018 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2930505Kleiner-Grote G, Risse JM, Friehs K (2018)PUB | DOI | WoS | PubMed | Europe PMC
Secretion of recombinant proteins from E. coli.
ENGINEERING IN LIFE SCIENCES 18(8): 532-550. -
2017 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2910668Friehs K, Schwarzhans JP, Luttermann T, Wibberg D, Winkler A, Hübner W, Huser T, Kalinowski J (2017)PUB | PDF | DOI | WoS | PubMed | Europe PMC
A Mitochondrial Autonomously Replicating Sequence from Pichia pastoris for Uniform High Level Recombinant Protein Production.
Frontiers in Microbiology 8: 780. -
2017 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2916444Schwarzhans JP, Luttermann T, Geier M, Kalinowski J, Friehs K (2017)PUB | DOI | WoS | PubMed | Europe PMC
Towards systems metabolic engineering in Pichia pastoris.
BIOTECHNOLOGY ADVANCES 35(6): 681-710. -
2017 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2908307Perez F, Risse JM, Friehs K, Wendisch VF (2017)PUB | DOI | WoS | PubMed | Europe PMC
Fermentative production of L-pipecolic acid from glucose and alternative carbon sources.
Biotechnology Journal 12(7): 1600646. -
2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2903655Schwarzhans JP, Wibberg D, Winkler A, Luttermann T, Kalinowski J, Friehs K (2016)PUB | PDF | DOI | WoS | PubMed | Europe PMC
Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector optimization.
Microbial Cell Factories 15(1): 84. -
2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2907679Schwarzhans JP, Wibberg D, Winkler A, Luttermann T, Kalinowski J, Friehs K (2016)PUB | PDF | DOI | WoS | PubMed | Europe PMC
Non-canonical integration events in Pichia pastoris encountered during standard transformation analysed with genome sequencing.
Scientific Reports 6(1): 38952. -
2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2903023Wetzel D, Müller J, Flaschel E, Friehs K, Risse JM (2016)PUB | DOI | WoS | PubMed | Europe PMC
Fed-batch production and secretion of streptavidin by Hansenula polymorpha: Evaluation of genetic factors and bioprocess development.
JOURNAL OF BIOTECHNOLOGY 225: 3-9. -
2016 | Zeitschriftenaufsatz | E-Veröff. vor dem Druck | PUB-ID: 2906700Holtmann D, Vernen F, Müller J, Kaden D, Risse JM, Friehs K, Dähne L, Stratmann A, Schrader J (2016)PUB | DOI | WoS
Effects of particle addition to Streptomyces cultivations to optimize the production of actinorhodin and streptavidin.
Sustainable Chemistry and Pharmacy 5: 67-71. -
2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2906197Müller J, Bruhn S, Flaschel E, Friehs K, Risse JM (2016)PUB | DOI | WoS | PubMed | Europe PMC
GAP promoter-based fed-batch production of highly bioactive core streptavidin by Pichia pastoris.
BIOTECHNOLOGY PROGRESS 32(4): 855-864. -
2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2900637Müller J, Wetzel D, Flaschel E, Friehs K, Risse JM (2016)PUB | DOI | WoS | PubMed | Europe PMC
Constitutive production and efficient secretion of soluble full-length streptavidin by an Escherichia coli 'leaky mutant'.
Journal of Biotechnology 221: 91-100. -
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2015 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2759080Grimm P, Risse JM, Müller J, Cholewa D, Beshay U, Friehs K, Flaschel E (2015)PUB | DOI | WoS | PubMed | Europe PMC
Applicability of Euglena gracilis for biorefineries demonstrated by the production of α-tocopherol and paramylon followed by anaerobic digestion.
Journal of Biotechnology 215: 72-79. -
2015 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2722001Schwarzhans JP, Cholewa D, Grimm P, Beshay U, Risse JM, Friehs K, Flaschel E (2015)PUB | DOI | WoS
Dependency of the fatty acid composition of Euglena gracilis on growth phase and culture conditions.
Journal of Applied Phycology 27(4): 1389-1399. -
2014 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2679910Selvamani RSV, Telaar M, Friehs K, Flaschel E (2014)PUB | PDF | DOI | WoS | PubMed | Europe PMC
Antibiotic-free segregational plasmid stabilization in Escherichia coli owing to the knockout of triosephosphate isomerase (tpiA).
Microbial Cell Factories 13(1): 58. -
2014 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2675672Selvamani RSV, Friehs K, Flaschel E (2014)PUB | DOI | WoS | PubMed | Europe PMC
Extracellular recombinant protein production under continuous culture conditions with Escherichia coli using an alternative plasmid selection mechanism.
Bioprocess and Biosystems Engineering 37(3): 401-413. -
2012 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2474324Santek B, Friehs K, Lotz M, Flaschel E (2012)PUB | DOI | WoS
Production of paramylon, a ss-1,3-glucan, by heterotrophic growth of Euglena gracilis on potato liquor in fed-batch and repeated-batch mode of cultivation.
Engineering in Life Sciences 12(1): 89-94. -
2010 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2401962Risse JM, Friehs K (2010)PUB
Sekretion und Affinitätschromatografie rekombinanter Proteine.
BIOspektrum 5: 550-552. -
2010 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1796536Sommer B, Friehs K, Flaschel E (2010)PUB | DOI | WoS | PubMed | Europe PMC
Efficient production of extracellular proteins with Escherichia coli by means of optimized coexpression of bacteriocin release proteins.
JOURNAL OF BIOTECHNOLOGY 145(4): 350-358. -
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2009 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1634165Sommer B, Friehs K, Flaschel E, Reck M, Stahl F, Scheper T (2009)PUB | DOI | WoS | PubMed | Europe PMC
Extracellular production and affinity purification of recombinant proteins with Escherichia coli using the versatility of the maltose binding protein.
JOURNAL OF BIOTECHNOLOGY 140(3-4): 194-202. -
2009 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1636091Beshay U, Miksch G, Friehs K, Flaschel E (2009)PUB | DOI | WoS | PubMed | Europe PMC
Integrated bioprocess for the production and purification of recombinant proteins by affinity chromatography in Escherichia coli.
BIOPROCESS AND BIOSYSTEMS ENGINEERING 32(2): 149-158. -
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2008 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1585611Wei N, Flaschel E, Friehs K, Nattkemper TW (2008)PUB | PDF | DOI | WoS | PubMed | Europe PMC
A machine vision system for automated non-invasive assessment of cell viability via dark field microscopy, wavelet feature selection and classification.
BMC Bioinformatics 9(1): 449. -
2008 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1587590Vijayendran C, Barsch A, Friehs K, Niehaus K, Becker A, Flaschel E (2008)PUB | PDF | DOI | WoS | PubMed | Europe PMC
Perceiving molecular evolution processes in Escherichia coli by comprehensive metabolite and gene expression profiling.
GENOME BIOLOGY 9(4): R72. -
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1594835Vijayendran C, Polen T, Wendisch VF, Friehs K, Niehaus K, Flaschel E (2007)PUB | DOI | WoS | PubMed | Europe PMC
The plasticity of global proteome and genome expression analyzed in closely related W3110 and MG1655 strains of a well-studied model organism, Escherichia coli-K12.
Journal of Biotechnology 128(4): 747-761. -
2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1595739Wei N, You J, Friehs K, Flaschel E, Nattkemper TW (2007)PUB | DOI | WoS | PubMed | Europe PMC
In situ dark field microscopy for on-line monitoring of yeast cultures.
BIOTECHNOLOGY LETTERS 29(3): 373-378. -
2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1593120Wei N, You J, Friehs K, Flaschel E, Nattkemper TW (2007)PUB | DOI | WoS | PubMed | Europe PMC
An in situ probe for on-line monitoring of cell density and viability on the basis of dark field microscopy in conjunction with image processing and supervised machine learning.
Biotechnology and Bioengineering 97(6): 1489-1500. -
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1631107Blaesen M, Friehs K, Flaschel E (2007)PUB | DOI | WoS | PubMed | Europe PMC
Recycling of bacterial biomass in a process of L-threonine production by means of a recombinant strain of Escherichia coli.
JOURNAL OF BIOTECHNOLOGY 132(4): 431-437. -
2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1631661Beshay U, Miksch G, Friehs K, Flaschel E (2007)PUB | DOI | WoS | PubMed | Europe PMC
Increasing the secretion ability of the kil gene for recombinant proteins in Escherichia coli by using a strong stationary-phase promoter.
BIOTECHNOLOGY LETTERS 29(12): 1893-1901. -
2006 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1600329Miksch G, Bettenworth F, Friehs K, Flaschel E, Saalbach A, Nattkemper TW (2006)PUB | DOI | WoS | PubMed | Europe PMC
A rapid reporter system using GFP as a reporter protein for identification and screening of synthetic stationary-phase promoters in Escherichia coli.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 70(2): 229-236. -
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2005 | Kurzbeitrag Konferenz / Poster | PUB-ID: 2906664Polen T, Wendisch VF, Friehs K, Niehaus K, Flaschel E, Vijayendran C (2005)PUB | WoS
The plasticity of global proteome and genome expression analysis in a well studied closely related model organism, Escherichia coli.
MOLECULAR & CELLULAR PROTEOMICS 4(8): S248-S248. -
2005 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1604386Arndt M, Kleist S, Miksch G, Friehs K, Flaschel E, Trierweiler J, Hitzmann B (2005)PUB | DOI | WoS
A feedforward-feedback substrate controller based on a Kalman filter for a fed-batch cultivation of Escherichia coli producing phytase.
COMPUTERS & CHEMICAL ENGINEERING 29(5): 1113-1120. -
2005 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1601074Miksch G, Bettenworth F, Friehs K, Flaschel E (2005)PUB | DOI | WoS | PubMed | Europe PMC
The sequence upstream of the -10 consensus sequence modulates the strength and induction time of stationary-phase promoters in Escherichia coli.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 69(3): 312-320. -
2005 | Konferenzbeitrag | Veröffentlicht | PUB-ID: 1601848Miksch G, Bettenworth F, Friehs K, Flaschel E, Saalbach A, Twellmann T, Nattkemper TW (2005)PUB | DOI | WoS | PubMed | Europe PMC
Libraries of synthetic stationary-phase and stress promoters as a tool for fine-tuning of expression of recombinant proteins in Escherichia coli.
In: Journal of Biotechnology. JOURNAL OF BIOTECHNOLOGY, 120. ELSEVIER SCIENCE BV: 25-37. -
2005 | Kurzbeitrag Konferenz / Poster | Veröffentlicht | PUB-ID: 1601124Vijayendran C, Polen T, Wendisch V, Friehs K, Niehaus K, Flaschel E (2005)PUB | WoS
The plasticity of global proteome and genome expression analysis in a well studied closely related model organism, Escherichia coli.
Molecular & Cellular Proteomics 4(8: Suppl. 1): S248. -
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2004 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1608382Lu YH, Knol JC, Linskens MHK, Friehs K, van Haastert PJM, Flaschel E (2004)PUB | DOI | WoS | PubMed | Europe PMC
Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium.
JOURNAL OF BIOTECHNOLOGY 108(3): 243-251. -
2004 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1609051Voß C, Schmidt T, Schleef M, Friehs K, Flaschel E (2004)PUB | DOI | WoS
Effect of ammonium chloride on plasmid DNA production in high cell density batch culture for biopharmaceutical use.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 79(1): 57-62. -
2004 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1606278Lu YH, Knol JC, Linskens MHK, Friehs K, van Haastert PJM, Flaschel E (2004)PUB | DOI | WoS | PubMed | Europe PMC
Cultivation of immobilized Dictyostelium discoideum for the production of soluble human Fas ligand.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 65(5): 547-552. -
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2003 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1609437Beshay U, Friehs K, Azzam AEM, Flaschel E (2003)PUB | DOI | WoS | PubMed | Europe PMC
Analysis of the behaviour of Dictyostelium discoideum in immobilised state by means of continuous cultivation.
BIOPROCESS AND BIOSYSTEMS ENGINEERING 26(2): 117-122. -
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2003 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1609656Voß C, Schmidt T, Schleef M, Friehs K, Flaschel E (2003)PUB | DOI | WoS | PubMed | Europe PMC
Production of supercoiled multimeric plasmid DNA for biopharmaceutical application.
JOURNAL OF BIOTECHNOLOGY 105(3): 205-213. -
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2002 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1880734Schierl F, Friehs K, Risse JM, Flaschel E (2002)PUB
Herstellung von Mediumskomponenten aus mikrobieller Biomasse.
Freiberger Forschungshefte A 866: 112-124. -
2002 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1613472Miksch G, Kleist S, Friehs K, Flaschel E (2002)PUB | DOI | WoS | PubMed | Europe PMC
Overexpression of the phytase from Escherichia coli and its extracellular production in bioreactors.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 59(6): 685-694. -
2001 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1880242Risse JM, Friehs K, Flaschel E (2001)PUB
Recycling of Biomass Resulting from Fermentation Processes with Bacillus licheniformis.
Chem. Eng. Technol. 24(10): 141-145. -
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1999 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1622862Miksch G, Neitzel R, Friehs K, Flaschel E (1999)PUB | DOI | WoS | PubMed | Europe PMC
High-level expression of a recombinant protein in Klebsiella planticola owing to induced secretion into the culture medium.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 51(5): 627-632. -
1999 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1621612Schmidt T, Friehs K, Schleef M, Voß C, Flaschel E (1999)PUB | DOI | WoS | PubMed | Europe PMC
Quantitative analysis of plasmid forms by agarose and capillary gel electrophoresis.
ANALYTICAL BIOCHEMISTRY 274(2): 235-240. -
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1997 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1637759Miksch G, Neitzel R, Fiedler E, Friehs K, Flaschel E (1997)PUB | DOI | WoS | PubMed | Europe PMC
Extracellular production of a hybrid beta-glucanase from Bacillus by Escherichia coli under different cultivation conditions in shaking cultures and bioreactors.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 47(2): 120-126. -
1997 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1627108Poppenborg L, Friehs K, Flaschel E (1997)PUB | DOI | WoS | PubMed | Europe PMC
The green fluorescent protein is a versatile reporter for bioprocess monitoring.
JOURNAL OF BIOTECHNOLOGY 58(2): 79-88. -
1996 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1628395Schmidt T, Friehs K, Flaschel E (1996)PUB | DOI | WoS | PubMed | Europe PMC
Rapid determination of plasmid copy number.
JOURNAL OF BIOTECHNOLOGY 49(1-3): 219-229. -
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1993 | Sammelwerksbeitrag | Veröffentlicht | PUB-ID: 1782559Reardon KF, Friehs K (1993)PUB | PDF | PubMed | Europe PMC
Parameters influencing the productivity of recombinant E. coli cultivations.
In: Bioprocess design and control. Aarts R (Ed); Advances in biochemical engineering, biotechnology ; 48, Berlin [u.a.]: Springer: 53-77. -
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1986 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1782542Friehs K, Schörgendorfer K, Schwab H, Lafferty RM (1986)PUB | PDF | DOI | WoS
Cloning and phenotypic expression in Escherichia coli of a Bacillus subtilis gene fragment coding for sucrose hydrolysis.
Journal of Biotechnology 3(5-6): 333-341.