The green fluorescent protein is a versatile reporter for bioprocess monitoring

Poppenborg L, Friehs K, Flaschel E (1997)
JOURNAL OF BIOTECHNOLOGY 58(2): 79-88.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
The green fluorescent protein (GFP) of Aequorea victor ia has become a convenient and versatile tool as a reporter protein in molecular cell biology and developmental biology. Here, it is shown that GFP may advantageously be used as a reporter system for bioprocess monitoring as well. Examples are given for monitoring fermentation as well as downstream processes for protein recovery. Thus, separation processes based on the application of affinity-fusion tags may be optimized in terms of the operational conditions by using GFP as a model target protein owing to facile screening by simple Visual inspection. This item is discussed together with the presentation of a novel fusion tag with strong affinity for metal-chelate ligands: hisactophilin, a histidine-rich protein of Dictyostelium discoideum. This tag is of particular interest for affinity separation processes requiring multiple sites of interaction like aqueous and reverse micellar two-phase extraction as well as precipitation. (C) 1997 Elsevier Science B.V.
Erscheinungsjahr
Zeitschriftentitel
JOURNAL OF BIOTECHNOLOGY
Band
58
Zeitschriftennummer
2
Seite
79-88
ISSN
PUB-ID

Zitieren

Poppenborg L, Friehs K, Flaschel E. The green fluorescent protein is a versatile reporter for bioprocess monitoring. JOURNAL OF BIOTECHNOLOGY. 1997;58(2):79-88.
Poppenborg, L., Friehs, K., & Flaschel, E. (1997). The green fluorescent protein is a versatile reporter for bioprocess monitoring. JOURNAL OF BIOTECHNOLOGY, 58(2), 79-88. doi:10.1016/S0168-1656(97)00134-X
Poppenborg, L., Friehs, K., and Flaschel, E. (1997). The green fluorescent protein is a versatile reporter for bioprocess monitoring. JOURNAL OF BIOTECHNOLOGY 58, 79-88.
Poppenborg, L., Friehs, K., & Flaschel, E., 1997. The green fluorescent protein is a versatile reporter for bioprocess monitoring. JOURNAL OF BIOTECHNOLOGY, 58(2), p 79-88.
L. Poppenborg, K. Friehs, and E. Flaschel, “The green fluorescent protein is a versatile reporter for bioprocess monitoring”, JOURNAL OF BIOTECHNOLOGY, vol. 58, 1997, pp. 79-88.
Poppenborg, L., Friehs, K., Flaschel, E.: The green fluorescent protein is a versatile reporter for bioprocess monitoring. JOURNAL OF BIOTECHNOLOGY. 58, 79-88 (1997).
Poppenborg, L, Friehs, Karl, and Flaschel, Erwin. “The green fluorescent protein is a versatile reporter for bioprocess monitoring”. JOURNAL OF BIOTECHNOLOGY 58.2 (1997): 79-88.

28 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Molecular display technology using yeast--arming technology.
Shibasaki S, Maeda H, Ueda M., Anal Sci 25(1), 2009
PMID: 19139571
Purification of GFP fusion proteins from transgenic plant cell cultures.
Peckham GD, Bugos RC, Su WW., Protein Expr Purif 49(2), 2006
PMID: 16682226
Rainbow tags: a visual tag system for recombinant protein expression and purification.
Finn RD, Kapelioukh I, Paine MJ., Biotechniques 38(3), 2005
PMID: 15789482
Fluorescent proteins as tools to aid protein production.
Su WW., Microb Cell Fact 4(1), 2005
PMID: 15850488
Immobilisation of a repressor protein for binding of plasmid DNA.
Hasche A, Voss C., J Chromatogr A 1080(1), 2005
PMID: 16013617
Purification of recombinant green fluorescent protein by three-phase partitioning.
Jain S, Singh R, Gupta MN., J Chromatogr A 1035(1), 2004
PMID: 15117077
Single-cell variability in growing Saccharomyces cerevisiae cell populations measured with automated flow cytometry.
Kacmar J, Zamamiri A, Carlson R, Abu-Absi NR, Srienc F., J Biotechnol 109(3), 2004
PMID: 15066762
Engineering green fluorescent protein as a dual functional tag.
Paramban RI, Bugos RC, Su WW., Biotechnol Bioeng 86(6), 2004
PMID: 15137081
Rapid monitoring of recombinant protein products: a comparison of current technologies.
Baker KN, Rendall MH, Patel A, Boyd P, Hoare M, Freedman RB, James DC., Trends Biotechnol 20(4), 2002
PMID: 11906746
Balancing GFP reporter plasmid quantity in large-scale transient transfections for recombinant anti-human Rhesus-D IgG1 synthesis.
Pick HM, Meissner P, Preuss AK, Tromba P, Vogel H, Wurm FM., Biotechnol Bioeng 79(6), 2002
PMID: 12209806
Green fluorescent protein is lighting up fungal biology.
Lorang JM, Tuori RP, Martinez JP, Sawyer TL, Redman RS, Rollins JA, Wolpert TJ, Johnson KB, Rodriguez RJ, Dickman MB, Ciuffetti LM., Appl Environ Microbiol 67(5), 2001
PMID: 11319072
Green fluorescent protein in Saccharomyces cerevisiae: real-time studies of the GAL1 promoter.
Li J, Wang S, VanDusen WJ, Schultz LD, George HA, Herber WK, Chae HJ, Bentley WE, Rao G., Biotechnol Bioeng 70(2), 2000
PMID: 10972930
Designing new metal affinity peptides by random mutagenesis of a natural metal-binding site.
Enzelberger MM, Minning S, Schmid RD., J Chromatogr A 898(1), 2000
PMID: 11185626

49 References

Daten bereitgestellt von Europe PubMed Central.

Solubilization mechanism of cytochrome c in sodium bis(2-ethylhexyl) sulfosuccinate water/oil microemulsion
Adachi, J. Phys. Chem. 97(), 1993
Preparation of technical grade polyethylene glycol (PEG) (Mr 20 000)-N6-(2-aminoethyl)-NADH by a procedure adaptable to large-scale synthesis
Bückmann, Biotechnol. Appl. Biochem. 9(), 1987
Green fluorescent protein as a marker for gene expression.
Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC., Science 263(5148), 1994
PMID: 8303295
Improved oligonucleotide site-directed mutagenesis using M13 vectors.
Carter P, Bedouelle H, Winter G., Nucleic Acids Res. 13(12), 1985
PMID: 2989795
Pest control by fluorescence
Chao, Nature 380(), 1996
FACS-optimized mutants of the green fluorescent protein (GFP).
Cormack BP, Valdivia RH, Falkow S., Gene 173(1 Spec No), 1996
PMID: 8707053
N-laurylbiotinamide as affinity surfactant.
Coughlin RW, Baclaski JB., Biotechnol. Prog. 6(4), 1990
PMID: 1370004
Improved green fluorescent protein by molecular evolution using DNA shuffling.
Crameri A, Whitehorn EA, Tate E, Stemmer WP., Nat. Biotechnol. 14(3), 1996
PMID: 9630892
Red-shifted excitation mutants of the green fluorescent protein.
Delagrave S, Hawtin RE, Silva CM, Yang MM, Youvan DC., Biotechnology (N.Y.) 13(2), 1995
PMID: 9634755
Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector.
Furste JP, Pansegrau W, Frank R, Blocker H, Scholz P, Bagdasarian M, Lanka E., Gene 48(1), 1986
PMID: 3549457
Structure of hisactophilin is similar to interleukin-1 beta and fibroblast growth factor.
Habazettl J, Gondol D, Wiltscheck R, Otlewski J, Schleicher M, Holak TA., Nature 359(6398), 1992
PMID: 1436061
Wavelength mutations and posttranslational autoxidation of green fluorescent protein.
Heim R, Prasher DC, Tsien RY., Proc. Natl. Acad. Sci. U.S.A. 91(26), 1994
PMID: 7809066
Improved green fluorescence.
Heim R, Cubitt AB, Tsien RY., Nature 373(6516), 1995
PMID: 7854443
Study of the simultaneous partitioning of proteins and surfactant in reverse micellar two-phase systems—potential impact upon purification efficiency
Hentsch, Bioseparation 6(), 1996
Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent
Hochuli, BioTechnology 6(), 1988
Enterohepatic chelating agents—I
Jones, J. Inorg. Nucl. Chem. 38(), 1975
Affinity-based reversed micellar protein extraction: I. principles and protein-ligand systems.
Kelley BD, Wang DI, Hatton TA., Biotechnol. Bioeng. 42(10), 1993
PMID: 18609669
Affinity-based reversed micellar protein extraction: II. effect of cosurfactant tail length.
Kelley BD, Wang DI, Hatton TA., Biotechnol. Bioeng. 42(10), 1993
PMID: 18609670
Amino acid sequence of human histidine-rich glycoprotein derived from the nucleotide sequence of its cDNA.
Koide T, Foster D, Yoshitake S, Davie EW., Biochemistry 25(8), 1986
PMID: 3011081
Metal affinity precipitation of proteins carrying genetically attached polyhistidine affinity tails.
Lilius G, Persson M, Bulow L, Mosbach K., Eur. J. Biochem. 198(2), 1991
PMID: 1904025
Immobilization and affinity purification of recombinant proteins using histidine peptide fusions.
Ljungquist C, Breitholtz A, Brink-Nilsson H, Moks T, Uhlen M, Nilsson B., Eur. J. Biochem. 186(3), 1989
PMID: 2514094
Crystal structure of the Aequorea victoria green fluorescent protein.
Ormo M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ., Science 273(5280), 1996
PMID: 8703075
Structure of actin binding proteins: insights about function at atomic resolution.
Pollard TD, Almo S, Quirk S, Vinson V, Lattman EE., Annu. Rev. Cell Biol. 10(), 1994
PMID: 7888177
Affinity extraction of proteins by means of reverse micellar phases containing a metal-chelating surfactant
Poppenborg, Biotechnol. Tech. 8(), 1994
Using GFP to see the light.
Prasher DC., Trends Genet. 11(8), 1995
PMID: 8585130
Hisactophilin, a histidine-rich actin-binding protein from Dictyostelium discoideum.
Scheel J, Ziegelbauer K, Kupke T, Humbel BM, Noegel AA, Gerisch G, Schleicher M., J. Biol. Chem. 264(5), 1989
PMID: 2914932
Enzyme purification by immobilized metal ion affinity partitioning--application to D-hydroxyisocaproate dehydrogenase.
Schustolla D, Deckwer WD, Schugerl K, Hustedt H., Bioseparation 3(2-3), 1992
PMID: 1369240
Measurement of protein using bicinchoninic acid.
Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC., Anal. Biochem. 150(1), 1985
PMID: 3843705
Sequence of a cDNA encoding a small polymorphic histidine- and alanine-rich protein from Plasmodium falciparum.
Stahl HD, Kemp DJ, Crewther PE, Scanlon DB, Woodrow G, Brown GV, Bianco AE, Anders RF, Coppel RL., Nucleic Acids Res. 13(21), 1985
PMID: 2415925
A fusion protein designed for noncovalent immobilization: stability, enzymatic activity, and use in an enzyme reactor.
Stempfer G, Holl-Neugebauer B, Kopetzki E, Rudolph R., Nat. Biotechnol. 14(4), 1996
PMID: 9630924
Improved refolding of an immobilized fusion protein.
Stempfer G, Holl-Neugebauer B, Rudolph R., Nat. Biotechnol. 14(3), 1996
PMID: 9630895
Monitoring transgenic plants using in vivo markers.
Stewart CN Jr., Nat. Biotechnol. 14(6), 1996
PMID: 9630968
Novel metal-affinity protein separations
Suh, ACS Symp. Ser. 427(), 1990
Metal affinity precipitation of proteins.
Van Dam ME, Wuenschell GE, Arnold FH., Biotechnol. Appl. Biochem. 11(5), 1989
PMID: 2508699
Homologous genes encode two distinct histidine-rich proteins in a cloned isolate of Plasmodium falciparum.
Wellems TE, Howard RJ., Proc. Natl. Acad. Sci. U.S.A. 83(16), 1986
PMID: 3016741
Bioaffinity separations using reversed micellar extraction
Woll, Biotechnol. Prog. 5(), 1989
Aqueous two-phase metal affinity extraction of heme proteins.
Wuenschell GE, Naranjo E, Arnold FH., Bioprocess engineering. 5(5), 1990
PMID: IND90053464
Chiral copper-chelate complexes alter selectivities in metal affinity protein partitioning.
Wuenschell GE, Wen E, Todd R, Shnek D, Arnold FH., J. Chromatogr. 543(2), 1991
PMID: 1880194
The molecular structure of green fluorescent protein.
Yang F, Moss LG, Phillips GN Jr., Nat. Biotechnol. 14(10), 1996
PMID: 9631087

AUTHOR UNKNOWN, 0

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 9383982
PubMed | Europe PMC

Suchen in

Google Scholar