The green fluorescent protein is a versatile reporter for bioprocess monitoring

Poppenborg L, Friehs K, Flaschel E (1997)
JOURNAL OF BIOTECHNOLOGY 58(2): 79-88.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
The green fluorescent protein (GFP) of Aequorea victor ia has become a convenient and versatile tool as a reporter protein in molecular cell biology and developmental biology. Here, it is shown that GFP may advantageously be used as a reporter system for bioprocess monitoring as well. Examples are given for monitoring fermentation as well as downstream processes for protein recovery. Thus, separation processes based on the application of affinity-fusion tags may be optimized in terms of the operational conditions by using GFP as a model target protein owing to facile screening by simple Visual inspection. This item is discussed together with the presentation of a novel fusion tag with strong affinity for metal-chelate ligands: hisactophilin, a histidine-rich protein of Dictyostelium discoideum. This tag is of particular interest for affinity separation processes requiring multiple sites of interaction like aqueous and reverse micellar two-phase extraction as well as precipitation. (C) 1997 Elsevier Science B.V.
Stichworte
GFP; hisactophilin; process monitoring; downstream processing; affinity precipitation; green fluorescent protein; fusion tag; metal-chelate affinity
Erscheinungsjahr
1997
Zeitschriftentitel
JOURNAL OF BIOTECHNOLOGY
Band
58
Ausgabe
2
Seite(n)
79-88
ISSN
0168-1656
Page URI
https://pub.uni-bielefeld.de/record/1627108

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Poppenborg L, Friehs K, Flaschel E. The green fluorescent protein is a versatile reporter for bioprocess monitoring. JOURNAL OF BIOTECHNOLOGY. 1997;58(2):79-88.
Poppenborg, L., Friehs, K., & Flaschel, E. (1997). The green fluorescent protein is a versatile reporter for bioprocess monitoring. JOURNAL OF BIOTECHNOLOGY, 58(2), 79-88. doi:10.1016/S0168-1656(97)00134-X
Poppenborg, L., Friehs, K., and Flaschel, E. (1997). The green fluorescent protein is a versatile reporter for bioprocess monitoring. JOURNAL OF BIOTECHNOLOGY 58, 79-88.
Poppenborg, L., Friehs, K., & Flaschel, E., 1997. The green fluorescent protein is a versatile reporter for bioprocess monitoring. JOURNAL OF BIOTECHNOLOGY, 58(2), p 79-88.
L. Poppenborg, K. Friehs, and E. Flaschel, “The green fluorescent protein is a versatile reporter for bioprocess monitoring”, JOURNAL OF BIOTECHNOLOGY, vol. 58, 1997, pp. 79-88.
Poppenborg, L., Friehs, K., Flaschel, E.: The green fluorescent protein is a versatile reporter for bioprocess monitoring. JOURNAL OF BIOTECHNOLOGY. 58, 79-88 (1997).
Poppenborg, L, Friehs, Karl, and Flaschel, Erwin. “The green fluorescent protein is a versatile reporter for bioprocess monitoring”. JOURNAL OF BIOTECHNOLOGY 58.2 (1997): 79-88.

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