Libraries of synthetic stationary-phase and stress promoters as a tool for fine-tuning of expression of recombinant proteins in Escherichia coli

Miksch G, Bettenworth F, Friehs K, Flaschel E, Saalbach A, Twellmann T, Nattkemper TW (2005)
JOURNAL OF BIOTECHNOLOGY 120(1): 25-37.

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Abstract / Bemerkung
Due to their induction characteristics stationary-phase promoters have a great potential in biotechnological processes for the production of heterologous proteins on a large-scale. In order to broaden the utility of stationary-phase promoters in bacterial expression systems and to create novel promoters induced by metabolic conditions, a library of synthetic stationary-phase/stress promoters for Escherichia coli was constructed. For designing the promoters the known -10 consensus sequence as well as the extended -10 region and an A/T-rich region downstream of the - 10 region were kept constant, while sequences from -37 to -14 were partially or completely randomized. For detection and selection of stationary-phase promoters GFP with enhanced fluorescence was used. The expression pattern of the GFP reporter system was compared with that of the LacZ reporter system. To screen and characterize colonies containing stationary-phase/stress promoters a bioinformatic approach was developed. In total, 33 promoters were selected which cover a broad range of promoter activities and induction times indicating that the strength of promoters can be modulated by partially randomizing the sequence upstream of the -10 region. The induction ratio of synthetic promoters at the transition from exponential to stationary-phase was from 4 to over 6000 and the induction time relative to the entrance into stationary-phase from - 1.4 to 2.7 h. Ninety-one percentage of the promoters had no or only low background activity during exponential growth. The broad variability of the promoters offers good possibilities for fine-tuning of gene expression and for applications in industrial bioprocesses. (c) 2005 Elsevier B.V All rights reserved.
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120
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1
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25-37
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Miksch G, Bettenworth F, Friehs K, et al. Libraries of synthetic stationary-phase and stress promoters as a tool for fine-tuning of expression of recombinant proteins in Escherichia coli. JOURNAL OF BIOTECHNOLOGY. 2005;120(1):25-37.
Miksch, G., Bettenworth, F., Friehs, K., Flaschel, E., Saalbach, A., Twellmann, T., & Nattkemper, T. W. (2005). Libraries of synthetic stationary-phase and stress promoters as a tool for fine-tuning of expression of recombinant proteins in Escherichia coli. JOURNAL OF BIOTECHNOLOGY, 120(1), 25-37. doi:10.1016/j.jbiotec.2005.04.027
Miksch, G., Bettenworth, F., Friehs, K., Flaschel, E., Saalbach, A., Twellmann, T., and Nattkemper, T. W. (2005). Libraries of synthetic stationary-phase and stress promoters as a tool for fine-tuning of expression of recombinant proteins in Escherichia coli. JOURNAL OF BIOTECHNOLOGY 120, 25-37.
Miksch, G., et al., 2005. Libraries of synthetic stationary-phase and stress promoters as a tool for fine-tuning of expression of recombinant proteins in Escherichia coli. JOURNAL OF BIOTECHNOLOGY, 120(1), p 25-37.
G. Miksch, et al., “Libraries of synthetic stationary-phase and stress promoters as a tool for fine-tuning of expression of recombinant proteins in Escherichia coli”, JOURNAL OF BIOTECHNOLOGY, vol. 120, 2005, pp. 25-37.
Miksch, G., Bettenworth, F., Friehs, K., Flaschel, E., Saalbach, A., Twellmann, T., Nattkemper, T.W.: Libraries of synthetic stationary-phase and stress promoters as a tool for fine-tuning of expression of recombinant proteins in Escherichia coli. JOURNAL OF BIOTECHNOLOGY. 120, 25-37 (2005).
Miksch, Gerd, Bettenworth, F, Friehs, Karl, Flaschel, Erwin, Saalbach, A, Twellmann, Thorsten, and Nattkemper, Tim Wilhelm. “Libraries of synthetic stationary-phase and stress promoters as a tool for fine-tuning of expression of recombinant proteins in Escherichia coli”. JOURNAL OF BIOTECHNOLOGY 120.1 (2005): 25-37.

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PMID: 29740742
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PMID: 29635744
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PMID: 30150540
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PMID: 25232540
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PMID: 23578899
Engineering dynamic pathway regulation using stress-response promoters.
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PMID: 19816658
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De Mey M, Maertens J, Boogmans S, Soetaert WK, Vandamme EJ, Cunin R, Foulquié-Moreno MR., BMC Biotechnol 10(), 2010
PMID: 20334648
Random mutagenesis of the PM promoter as a powerful strategy for improvement of recombinant-gene expression.
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Manufacturing of recombinant therapeutic proteins in microbial systems.
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PMID: 16892246

60 References

Daten bereitgestellt von Europe PubMed Central.

A new family of sugar-inducible expression vectors for Escherichia coli.
Cagnon C, Valverde V, Masson JM., Protein Eng. 4(7), 1991
PMID: 1798708
Characterization of a pH-inducible promoter system for high-level expression of recombinant proteins in Escherichia coli.
Chou CH, Aristidou AA, Meng SY, Bennett GN, San KY., Biotechnol. Bioeng. 47(2), 1995
PMID: 18623392
Characterization of a pH-inducible promoter system for high-level expression of recombinant proteins in Escherichia coli.
Chou CH, Aristidou AA, Meng SY, Bennett GN, San KY., Biotechnol. Bioeng. 47(2), 1995
PMID: 18623392
Improved green fluorescent protein by molecular evolution using DNA shuffling.
Crameri A, Whitehorn EA, Tate E, Stemmer WP., Nat. Biotechnol. 14(3), 1996
PMID: 9630892
A consensus structure for sigma S-dependent promoters.
Espinosa-Urgel M, Chamizo C, Tormo A., Mol. Microbiol. 21(3), 1996
PMID: 8866487
Promoter recognition and discrimination by EsigmaS RNA polymerase.
Gaal T, Ross W, Estrem ST, Nguyen LH, Burgess RR, Gourse RL., Mol. Microbiol. 42(4), 2001
PMID: 11737638
Minimizing proteolysis in Escherichia coli: genetic solutions.
Gottesman S., Meth. Enzymol. 185(), 1990
PMID: 2199774
Prokaryotic promoters in biotechnology.
Goldstein MA, Doi RH., Biotechnol Annu Rev 1(), 1995
PMID: 9704086
Minimizing proteolysis in Escherichia coli: genetic solutions.
Gottesman S., Meth. Enzymol. 185(), 1990
PMID: 2199774
High-level gene expression in Escherichia coli
Gross, Chimicaoggi March (), 1989

AUTHOR UNKNOWN, 0
Dual regulation of the ugp operon by phosphate and carbon starvation at two interspaced promoters.
Kasahara M, Makino K, Amemura M, Nakata A, Shinagawa H., J. Bacteriol. 173(2), 1991
PMID: 1987150
Characterization of the nar promoter to use as an inducible promoter
Lee, Biotechnol. Lett. 18(), 1996
The role of the sigma factor sigma S (KatF) in bacterial global regulation.
Loewen PC, Hengge-Aronis R., Annu. Rev. Microbiol. 48(), 1994
PMID: 7826018
Regulation in the rpoS regulon of Escherichia coli.
Loewen PC, Hu B, Strutinsky J, Sparling R., Can. J. Microbiol. 44(8), 1998
PMID: 9830102
Efficient production of the C-terminal domain of secretory leukoprotease inhibitor as a thrombin-cleavable fusion protein in Escherichia coli.
Masuda K, Kamimura T, Kanesaki M, Ishii K, Imaizumi A, Sugiyama T, Suzuki Y, Ohtsuka E., Protein Eng. 9(1), 1996
PMID: 9053897

AUTHOR UNKNOWN, 0

AUTHOR UNKNOWN, 0

Miller, 1972
Current status of secretion of foreign proteins by microorganisms
Nicaud, J. Biotechnol. 3(), 1986
Engineering proteins to facilitate bioprocessing.
Nygren PA, Stahl S, Uhlen M., Trends Biotechnol. 12(5), 1994
PMID: 7764901
Synthesis and secretion of human epidermal growth factor by Escherichia coli.
Oka T, Sakamoto S, Miyoshi K, Fuwa T, Yoda K, Yamasaki M, Tamura G, Miyake T., Proc. Natl. Acad. Sci. U.S.A. 82(21), 1985
PMID: 3903748
Recent advances in heterologous gene expression in Escherichia coli.
Olins PO, Lee SC., Curr. Opin. Biotechnol. 4(5), 1993
PMID: 7764200
A threshold selection method from grey level histograms
Otsu, IEEE Trans. System Man Cybernetics 9(), 1979
High level heterologous expression in E. coli using the anaerobically-activated nirB promoter.
Oxer MD, Bentley CM, Doyle JG, Peakman TC, Charles IG, Makoff AJ., Nucleic Acids Res. 19(11), 1991
PMID: 2057350

Sambrook, 1989
New insights into mRNA decoding--implications for heterologous protein synthesis.
Santos MA, Tuite MF., Trends Biotechnol. 11(12), 1993
PMID: 7764419

AUTHOR UNKNOWN, 0
Classification and strength measurement of stationary-phase promoters by use of a newly developed promoter cloning vector.
Shimada T, Makinoshima H, Ogawa Y, Miki T, Maeda M, Ishihama A., J. Bacteriol. 186(21), 2004
PMID: 15489422
A novel phosphate-regulated expression vector in Escherichia coli.
Su TZ, Schweizer H, Oxender DL., Gene 90(1), 1990
PMID: 2379833
Structure of the 5' upstream region and the regulation of the rpoS gene of Escherichia coli.
Takayanagi Y, Tanaka K, Takahashi H., Mol. Gen. Genet. 243(5), 1994
PMID: 8208244
Sequences in the -35 region of Escherichia coli rpoS-dependent genes promote transcription by E sigma S.
Wise A, Brems R, Ramakrishnan V, Villarejo M., J. Bacteriol. 178(10), 1996
PMID: 8631665

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