73 Publikationen
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2021 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2957097Shankar R, Schäffer N, Schmeer M, Friehs K, Risse JM, Schleef M. Recombinant expression of Barnase in Escherichia coli and its application in plasmid purification. Microbial Cell Factories. 2021;20: 171.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2021 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2958416Luttermann T, Rückert C, Wibberg D, et al. Establishment of a near-contiguous genome sequence of the citric acid producing yeast DSM 3286 with resolution of rDNA clusters and telomeres. NAR Genomics and Bioinformatics. 2021;3(4):13.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2021 | Patent | PUB-ID: 2965425Risse JM, Friehs K, Wedde S, Hahn M, Ludwig B. Expression of collagen peptide components in procaryotic systems. 11.11.2021.PUB
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2021 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2957893Shankar R, Schafer N, Schmeer M, Risse JM, Friehs K, Schleef M. Correction to: Recombinant expression of Barnase in Escherichia coli and its application in plasmid purifcation. Microbial Cell Factories. 2021;20(1): 190.PUB | DOI | WoS | PubMed | Europe PMC
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2020 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2945772Heß M, Bloess S, Risse JM, Friehs K, Fischer von Mollard G. Recombinant expression of an l-amino acid oxidase from the fungus Hebeloma cylindrosporum in Pichia pastoris including fermentation. MicrobiologyOpen. 2020;9(10): e1112.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2020 | Datenpublikation | PUB-ID: 2944932Heß M, Bloess S, Risse JM, Friehs K, Fischer von Mollard G. Data for 'Recombinant expression of an L-amino acid oxidase from the fungus Hebeloma cylindrosporum in Pichia pastoris including fermentation'. Bielefeld University; 2020.PUB | Dateien verfügbar | DOI
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2018 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2930505Kleiner-Grote G, Risse JM, Friehs K. Secretion of recombinant proteins from E. coli. ENGINEERING IN LIFE SCIENCES. 2018;18(8):532-550.PUB | DOI | WoS | PubMed | Europe PMC
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2017 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2910668Friehs K, Schwarzhans JP, Luttermann T, et al. A Mitochondrial Autonomously Replicating Sequence from Pichia pastoris for Uniform High Level Recombinant Protein Production. Frontiers in Microbiology. 2017;8: 780.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2017 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2916444Schwarzhans JP, Luttermann T, Geier M, Kalinowski J, Friehs K. Towards systems metabolic engineering in Pichia pastoris. BIOTECHNOLOGY ADVANCES. 2017;35(6):681-710.PUB | DOI | WoS | PubMed | Europe PMC
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2017 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2908307Perez F, Risse JM, Friehs K, Wendisch VF. Fermentative production of L-pipecolic acid from glucose and alternative carbon sources. Biotechnology Journal. 2017;12(7): 1600646.PUB | DOI | WoS | PubMed | Europe PMC
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2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2903655Schwarzhans JP, Wibberg D, Winkler A, Luttermann T, Kalinowski J, Friehs K. Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector optimization. Microbial Cell Factories. 2016;15(1): 84.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2907679Schwarzhans JP, Wibberg D, Winkler A, Luttermann T, Kalinowski J, Friehs K. Non-canonical integration events in Pichia pastoris encountered during standard transformation analysed with genome sequencing. Scientific Reports. 2016;6(1): 38952.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2903023Wetzel D, Müller J, Flaschel E, Friehs K, Risse JM. Fed-batch production and secretion of streptavidin by Hansenula polymorpha: Evaluation of genetic factors and bioprocess development. JOURNAL OF BIOTECHNOLOGY. 2016;225:3-9.PUB | DOI | WoS | PubMed | Europe PMC
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2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2906197Müller J, Bruhn S, Flaschel E, Friehs K, Risse JM. GAP promoter-based fed-batch production of highly bioactive core streptavidin by Pichia pastoris. BIOTECHNOLOGY PROGRESS. 2016;32(4):855-864.PUB | DOI | WoS | PubMed | Europe PMC
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2016 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2900637Müller J, Wetzel D, Flaschel E, Friehs K, Risse JM. Constitutive production and efficient secretion of soluble full-length streptavidin by an Escherichia coli 'leaky mutant'. Journal of Biotechnology. 2016;221:91-100.PUB | DOI | WoS | PubMed | Europe PMC
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2015 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2759080Grimm P, Risse JM, Müller J, et al. Applicability of Euglena gracilis for biorefineries demonstrated by the production of α-tocopherol and paramylon followed by anaerobic digestion. Journal of Biotechnology. 2015;215:72-79.PUB | DOI | WoS | PubMed | Europe PMC
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2014 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2679910Selvamani RSV, Telaar M, Friehs K, Flaschel E. Antibiotic-free segregational plasmid stabilization in Escherichia coli owing to the knockout of triosephosphate isomerase (tpiA). Microbial Cell Factories. 2014;13(1): 58.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2014 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2675672Selvamani RSV, Friehs K, Flaschel E. Extracellular recombinant protein production under continuous culture conditions with Escherichia coli using an alternative plasmid selection mechanism. Bioprocess and Biosystems Engineering. 2014;37(3):401-413.PUB | DOI | WoS | PubMed | Europe PMC
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2012 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2474324Santek B, Friehs K, Lotz M, Flaschel E. Production of paramylon, a ss-1,3-glucan, by heterotrophic growth of Euglena gracilis on potato liquor in fed-batch and repeated-batch mode of cultivation. Engineering in Life Sciences. 2012;12(1):89-94.PUB | DOI | WoS
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2010 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 2401962Risse JM, Friehs K. Sekretion und Affinitätschromatografie rekombinanter Proteine. BIOspektrum. 2010;5:550-552.PUB
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2010 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1796536Sommer B, Friehs K, Flaschel E. Efficient production of extracellular proteins with Escherichia coli by means of optimized coexpression of bacteriocin release proteins. JOURNAL OF BIOTECHNOLOGY. 2010;145(4):350-358.PUB | DOI | WoS | PubMed | Europe PMC
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2009 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1636091Beshay U, Miksch G, Friehs K, Flaschel E. Integrated bioprocess for the production and purification of recombinant proteins by affinity chromatography in Escherichia coli. BIOPROCESS AND BIOSYSTEMS ENGINEERING. 2009;32(2):149-158.PUB | DOI | WoS | PubMed | Europe PMC
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2009 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1634165Sommer B, Friehs K, Flaschel E, Reck M, Stahl F, Scheper T. Extracellular production and affinity purification of recombinant proteins with Escherichia coli using the versatility of the maltose binding protein. JOURNAL OF BIOTECHNOLOGY. 2009;140(3-4):194-202.PUB | DOI | WoS | PubMed | Europe PMC
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2008 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1585611Wei N, Flaschel E, Friehs K, Nattkemper TW. A machine vision system for automated non-invasive assessment of cell viability via dark field microscopy, wavelet feature selection and classification. BMC Bioinformatics. 2008;9(1):449.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2008 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1587590Vijayendran C, Barsch A, Friehs K, Niehaus K, Becker A, Flaschel E. Perceiving molecular evolution processes in Escherichia coli by comprehensive metabolite and gene expression profiling. GENOME BIOLOGY. 2008;9(4):R72.PUB | PDF | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1594835Vijayendran C, Polen T, Wendisch VF, Friehs K, Niehaus K, Flaschel E. The plasticity of global proteome and genome expression analyzed in closely related W3110 and MG1655 strains of a well-studied model organism, Escherichia coli-K12. Journal of Biotechnology. 2007;128(4):747-761.PUB | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1595739Wei N, You J, Friehs K, Flaschel E, Nattkemper TW. In situ dark field microscopy for on-line monitoring of yeast cultures. BIOTECHNOLOGY LETTERS. 2007;29(3):373-378.PUB | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1593120Wei N, You J, Friehs K, Flaschel E, Nattkemper TW. An in situ probe for on-line monitoring of cell density and viability on the basis of dark field microscopy in conjunction with image processing and supervised machine learning. Biotechnology and Bioengineering. 2007;97(6):1489-1500.PUB | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1631107Blaesen M, Friehs K, Flaschel E. Recycling of bacterial biomass in a process of L-threonine production by means of a recombinant strain of Escherichia coli. JOURNAL OF BIOTECHNOLOGY. 2007;132(4):431-437.PUB | DOI | WoS | PubMed | Europe PMC
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2007 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1631661Beshay U, Miksch G, Friehs K, Flaschel E. Increasing the secretion ability of the kil gene for recombinant proteins in Escherichia coli by using a strong stationary-phase promoter. BIOTECHNOLOGY LETTERS. 2007;29(12):1893-1901.PUB | DOI | WoS | PubMed | Europe PMC
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2006 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1600329Miksch G, Bettenworth F, Friehs K, Flaschel E, Saalbach A, Nattkemper TW. A rapid reporter system using GFP as a reporter protein for identification and screening of synthetic stationary-phase promoters in Escherichia coli. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2006;70(2):229-236.PUB | DOI | WoS | PubMed | Europe PMC
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2005 | Kurzbeitrag Konferenz / Poster | PUB-ID: 2906664Polen T, Wendisch VF, Friehs K, Niehaus K, Flaschel E, Vijayendran C. The plasticity of global proteome and genome expression analysis in a well studied closely related model organism, Escherichia coli. MOLECULAR & CELLULAR PROTEOMICS. 2005;4(8):S248-S248.PUB | WoS
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2005 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1604386Arndt M, Kleist S, Miksch G, et al. A feedforward-feedback substrate controller based on a Kalman filter for a fed-batch cultivation of Escherichia coli producing phytase. COMPUTERS & CHEMICAL ENGINEERING. 2005;29(5):1113-1120.PUB | DOI | WoS
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2005 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1601074Miksch G, Bettenworth F, Friehs K, Flaschel E. The sequence upstream of the -10 consensus sequence modulates the strength and induction time of stationary-phase promoters in Escherichia coli. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2005;69(3):312-320.PUB | DOI | WoS | PubMed | Europe PMC
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2005 | Konferenzbeitrag | Veröffentlicht | PUB-ID: 1601848Miksch G, Bettenworth F, Friehs K, et al. Libraries of synthetic stationary-phase and stress promoters as a tool for fine-tuning of expression of recombinant proteins in Escherichia coli. In: Journal of Biotechnology. JOURNAL OF BIOTECHNOLOGY. Vol 120. ELSEVIER SCIENCE BV; 2005: 25-37.PUB | DOI | WoS | PubMed | Europe PMC
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2005 | Kurzbeitrag Konferenz / Poster | Veröffentlicht | PUB-ID: 1601124Vijayendran C, Polen T, Wendisch V, Friehs K, Niehaus K, Flaschel E. The plasticity of global proteome and genome expression analysis in a well studied closely related model organism, Escherichia coli. Molecular & Cellular Proteomics. 2005;4(8: Suppl. 1):S248.PUB | WoS
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2004 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1609051Voß C, Schmidt T, Schleef M, Friehs K, Flaschel E. Effect of ammonium chloride on plasmid DNA production in high cell density batch culture for biopharmaceutical use. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY. 2004;79(1):57-62.PUB | DOI | WoS
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2004 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1608382Lu YH, Knol JC, Linskens MHK, Friehs K, van Haastert PJM, Flaschel E. Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium. JOURNAL OF BIOTECHNOLOGY. 2004;108(3):243-251.PUB | DOI | WoS | PubMed | Europe PMC
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2004 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1606278Lu YH, Knol JC, Linskens MHK, Friehs K, van Haastert PJM, Flaschel E. Cultivation of immobilized Dictyostelium discoideum for the production of soluble human Fas ligand. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2004;65(5):547-552.PUB | DOI | WoS | PubMed | Europe PMC
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2003 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1609437Beshay U, Friehs K, Azzam AEM, Flaschel E. Analysis of the behaviour of Dictyostelium discoideum in immobilised state by means of continuous cultivation. BIOPROCESS AND BIOSYSTEMS ENGINEERING. 2003;26(2):117-122.PUB | DOI | WoS | PubMed | Europe PMC
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2003 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1609656Voß C, Schmidt T, Schleef M, Friehs K, Flaschel E. Production of supercoiled multimeric plasmid DNA for biopharmaceutical application. JOURNAL OF BIOTECHNOLOGY. 2003;105(3):205-213.PUB | DOI | WoS | PubMed | Europe PMC
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2002 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1880734Schierl F, Friehs K, Risse JM, Flaschel E. Herstellung von Mediumskomponenten aus mikrobieller Biomasse. Freiberger Forschungshefte. 2002;A 866:112-124.PUB
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2002 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1613472Miksch G, Kleist S, Friehs K, Flaschel E. Overexpression of the phytase from Escherichia coli and its extracellular production in bioreactors. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2002;59(6):685-694.PUB | DOI | WoS | PubMed | Europe PMC
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2001 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1880242Risse JM, Friehs K, Flaschel E. Recycling of Biomass Resulting from Fermentation Processes with Bacillus licheniformis. Chem. Eng. Technol. 2001;24(10):141-145.PUB
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1999 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1621612Schmidt T, Friehs K, Schleef M, Voß C, Flaschel E. Quantitative analysis of plasmid forms by agarose and capillary gel electrophoresis. ANALYTICAL BIOCHEMISTRY. 1999;274(2):235-240.PUB | DOI | WoS | PubMed | Europe PMC
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1999 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1622862Miksch G, Neitzel R, Friehs K, Flaschel E. High-level expression of a recombinant protein in Klebsiella planticola owing to induced secretion into the culture medium. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 1999;51(5):627-632.PUB | DOI | WoS | PubMed | Europe PMC
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1997 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1637759Miksch G, Neitzel R, Fiedler E, Friehs K, Flaschel E. Extracellular production of a hybrid beta-glucanase from Bacillus by Escherichia coli under different cultivation conditions in shaking cultures and bioreactors. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 1997;47(2):120-126.PUB | DOI | WoS | PubMed | Europe PMC
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1997 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1627108Poppenborg L, Friehs K, Flaschel E. The green fluorescent protein is a versatile reporter for bioprocess monitoring. JOURNAL OF BIOTECHNOLOGY. 1997;58(2):79-88.PUB | DOI | WoS | PubMed | Europe PMC
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1996 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1628395Schmidt T, Friehs K, Flaschel E. Rapid determination of plasmid copy number. JOURNAL OF BIOTECHNOLOGY. 1996;49(1-3):219-229.PUB | DOI | WoS | PubMed | Europe PMC
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1993 | Sammelwerksbeitrag | Veröffentlicht | PUB-ID: 1782559Reardon KF, Friehs K. Parameters influencing the productivity of recombinant E. coli cultivations. In: Aarts R, ed. Bioprocess design and control. Advances in biochemical engineering, biotechnology ; 48. Berlin [u.a.]: Springer; 1993: 53-77.PUB | PDF | PubMed | Europe PMC
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1986 | Zeitschriftenaufsatz | Veröffentlicht | PUB-ID: 1782542Friehs K, Schörgendorfer K, Schwab H, Lafferty RM. Cloning and phenotypic expression in Escherichia coli of a Bacillus subtilis gene fragment coding for sucrose hydrolysis. Journal of Biotechnology. 1986;3(5-6):333-341.PUB | PDF | DOI | WoS