Draft whole genome sequence of the cyanide-degrading bacterium Pseudomonas pseudoalcaligenes CECT5344

Luque-Almagro VM, Acera F, Igeño MI, Wibberg D, Roldán MD, Sáez LP, Hennig M, Quesada A, Huertas MJ, Blom J, Merchán F, et al. (2013)
Environmental Microbiology 15(1): 253-270.

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Luque-Almagro, Víctor M; Acera, Felipe; Igeño, Ma Isabel; Wibberg, DanielUniBi; Roldán, Ma Dolores; Sáez, Lara P; Hennig, Magdalena; Quesada, Alberto; Huertas, Ma José; Blom, JochenUniBi; Merchán, Faustino; Escribano, Ma Paz
Abstract / Bemerkung
Pseudomonas pseudoalcaligenes CECT5344 is a Gram-negative bacterium able to tolerate cyanide and to use it as the sole nitrogen source. We report here the first draft of the whole genome sequence of a P. pseudoalcaligenes strain that assimilates cyanide. Three aspects are specially emphasized in this manuscript. First, some generalities of the genome are shown and discussed in the context of other Pseudomonadaceae genomes, including genome size, G + C content, core genome and singletons among other features. Second, the genome is analysed in the context of cyanide metabolism, describing genes probably involved in cyanide assimilation, like those encoding nitrilases, and genes related to cyanide resistance, like the cio genes encoding the cyanide insensitive oxidases. Finally, the presence of genes probably involved in other processes with a great biotechnological potential like production of bioplastics and biodegradation of pollutants also is discussed.
Environmental Microbiology
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Luque-Almagro VM, Acera F, Igeño MI, et al. Draft whole genome sequence of the cyanide-degrading bacterium Pseudomonas pseudoalcaligenes CECT5344. Environmental Microbiology. 2013;15(1):253-270.
Luque-Almagro, V. M., Acera, F., Igeño, M. I., Wibberg, D., Roldán, M. D., Sáez, L. P., Hennig, M., et al. (2013). Draft whole genome sequence of the cyanide-degrading bacterium Pseudomonas pseudoalcaligenes CECT5344. Environmental Microbiology, 15(1), 253-270. doi:10.1111/j.1462-2920.2012.02875.x
Luque-Almagro, Víctor M, Acera, Felipe, Igeño, Ma Isabel, Wibberg, Daniel, Roldán, Ma Dolores, Sáez, Lara P, Hennig, Magdalena, et al. 2013. “Draft whole genome sequence of the cyanide-degrading bacterium Pseudomonas pseudoalcaligenes CECT5344”. Environmental Microbiology 15 (1): 253-270.
Luque-Almagro, V. M., Acera, F., Igeño, M. I., Wibberg, D., Roldán, M. D., Sáez, L. P., Hennig, M., Quesada, A., Huertas, M. J., Blom, J., et al. (2013). Draft whole genome sequence of the cyanide-degrading bacterium Pseudomonas pseudoalcaligenes CECT5344. Environmental Microbiology 15, 253-270.
Luque-Almagro, V.M., et al., 2013. Draft whole genome sequence of the cyanide-degrading bacterium Pseudomonas pseudoalcaligenes CECT5344. Environmental Microbiology, 15(1), p 253-270.
V.M. Luque-Almagro, et al., “Draft whole genome sequence of the cyanide-degrading bacterium Pseudomonas pseudoalcaligenes CECT5344”, Environmental Microbiology, vol. 15, 2013, pp. 253-270.
Luque-Almagro, V.M., Acera, F., Igeño, M.I., Wibberg, D., Roldán, M.D., Sáez, L.P., Hennig, M., Quesada, A., Huertas, M.J., Blom, J., Merchán, F., Escribano, M.P., Jaenicke, S., Estepa, J., Guijo, M.I., Martínez-Luque, M., Macías, D., Szczepanowski, R., Becerra, G., Ramirez, S., Carmona, M.I., Gutiérrez, O., Manso, I., Pühler, A., Castillo, F., Moreno-Vivián, C., Schlüter, A., Blasco, R.: Draft whole genome sequence of the cyanide-degrading bacterium Pseudomonas pseudoalcaligenes CECT5344. Environmental Microbiology. 15, 253-270 (2013).
Luque-Almagro, Víctor M, Acera, Felipe, Igeño, Ma Isabel, Wibberg, Daniel, Roldán, Ma Dolores, Sáez, Lara P, Hennig, Magdalena, Quesada, Alberto, Huertas, Ma José, Blom, Jochen, Merchán, Faustino, Escribano, Ma Paz, Jaenicke, Sebastian, Estepa, Jessica, Guijo, Ma Isabel, Martínez-Luque, Manuel, Macías, Daniel, Szczepanowski, Rafael, Becerra, Gracia, Ramirez, Silvia, Carmona, Ma Isabel, Gutiérrez, Oscar, Manso, Isabel, Pühler, Alfred, Castillo, Francisco, Moreno-Vivián, Conrado, Schlüter, Andreas, and Blasco, Rafael. “Draft whole genome sequence of the cyanide-degrading bacterium Pseudomonas pseudoalcaligenes CECT5344”. Environmental Microbiology 15.1 (2013): 253-270.

13 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Putative small RNAs controlling detoxification of industrial cyanide-containing wastewaters by Pseudomonas pseudoalcaligenes CECT5344.
Olaya-Abril A, Luque-Almagro VM, Pérez MD, López CM, Amil F, Cabello P, Sáez LP, Moreno-Vivián C, Roldán MD., PLoS One 14(2), 2019
PMID: 30735537
Cyanate Assimilation by the Alkaliphilic Cyanide-Degrading Bacterium Pseudomonas pseudoalcaligenes CECT5344: Mutational Analysis of the cyn Gene Cluster.
Sáez LP, Cabello P, Ibáñez MI, Luque-Almagro VM, Roldán MD, Moreno-Vivián C., Int J Mol Sci 20(12), 2019
PMID: 31226739
Assimilation of cyanide and cyano-derivatives by Pseudomonas pseudoalcaligenes CECT5344: from omic approaches to biotechnological applications.
Cabello P, Luque-Almagro VM, Olaya-Abril A, Sáez LP, Moreno-Vivián C, Roldán MD., FEMS Microbiol Lett 365(6), 2018
PMID: 29438505
Quantitative proteomic analysis of Pseudomonas pseudoalcaligenes CECT5344 in response to industrial cyanide-containing wastewaters using Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS).
Ibáñez MI, Cabello P, Luque-Almagro VM, Sáez LP, Olaya A, Sánchez de Medina V, Luque de Castro MD, Moreno-Vivián C, Roldán MD., PLoS One 12(3), 2017
PMID: 28253357
A Cyanide-Induced 3-Cyanoalanine Nitrilase in the Cyanide-Assimilating Bacterium Pseudomonas pseudoalcaligenes Strain CECT 5344.
Acera F, Carmona MI, Castillo F, Quesada A, Blasco R., Appl Environ Microbiol 83(9), 2017
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Biodegradation of cyanide wastes from mining and jewellery industries.
Luque-Almagro VM, Moreno-Vivián C, Roldán MD., Curr Opin Biotechnol 38(), 2016
PMID: 26745356
Comparative genome analyses of Serratia marcescens FS14 reveals its high antagonistic potential.
Li P, Kwok AH, Jiang J, Ran T, Xu D, Wang W, Leung FC., PLoS One 10(4), 2015
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Pseudomonas pseudoalcaligenes CECT5344, a cyanide-degrading bacterium with by-product (polyhydroxyalkanoates) formation capacity.
Manso Cobos I, Ibáñez García MI, de la Peña Moreno F, Sáez Melero LP, Luque-Almagro VM, Castillo Rodríguez F, Roldán Ruiz MD, Prieto Jiménez MA, Moreno Vivián C., Microb Cell Fact 14(), 2015
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A novel cyanide-inducible gene cluster helps protect Pseudomonas aeruginosa from cyanide.
Frangipani E, Pérez-Martínez I, Williams HD, Cherbuin G, Haas D., Environ Microbiol Rep 6(1), 2014
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Complete genome sequence of the cyanide-degrading bacterium Pseudomonas pseudoalcaligenes CECT5344.
Wibberg D, Luque-Almagro VM, Igeño MI, Bremges A, Roldán MD, Merchán F, Sáez LP, Guijo MI, Manso MI, Macías D, Cabello P, Becerra G, Ibáñez MI, Carmona MI, Escribano MM, Castillo F, Sczyrba A, Moreno-Vivián C, Blasco R, Pühler A, Schlüter A., J Biotechnol 175(), 2014
PMID: 24553071

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