Uncovering Microbiome Adaptations in a Full-Scale Biogas Plant: Insights from MAG-Centric Metagenomics and Metaproteomics

Hassa J, Tubbesing TJ, Maus I, Heyer R, Benndorf D, Effenberger M, Henke C, Osterholz B, Beckstette M, Pühler A, Sczyrba A, et al. (2023)
Microorganisms 11(10): 2412.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
The current focus on renewable energy in global policy highlights the importance of methane production from biomass through anaerobic digestion (AD). To improve biomass digestion while ensuring overall process stability, microbiome-based management strategies become more important. In this study, metagenomes and metaproteomes were used for metagenomically assembled genome (MAG)-centric analyses to investigate a full-scale biogas plant consisting of three differentially operated digesters. Microbial communities were analyzed regarding their taxonomic composition, functional potential, as well as functions expressed on the proteome level. Different abundances of genes and enzymes related to the biogas process could be mostly attributed to different process parameters. Individual MAGs exhibiting different abundances in the digesters were studied in detail, and their roles in the hydrolysis, acidogenesis and acetogenesis steps of anaerobic digestion could be assigned. Methanoculleus thermohydrogenotrophicum was an active hydrogenotrophic methanogen in all three digesters, whereas Methanothermobacter wolfeii was more prevalent at higher process temperatures. Further analysis focused on MAGs, which were abundant in all digesters, indicating their potential to ensure biogas process stability. The most prevalent MAG belonged to the class Limnochordia; this MAG was ubiquitous in all three digesters and exhibited activity in numerous pathways related to different steps of AD.
Stichworte
biogas microbiome; biogas process chain; anaerobic digestion; metagenomic binning; metagenome analyses; metaproteome analyses
Erscheinungsjahr
2023
Zeitschriftentitel
Microorganisms
Band
11
Ausgabe
10
Art.-Nr.
2412
eISSN
2076-2607
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Universität Bielefeld gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2983209

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Hassa J, Tubbesing TJ, Maus I, et al. Uncovering Microbiome Adaptations in a Full-Scale Biogas Plant: Insights from MAG-Centric Metagenomics and Metaproteomics. Microorganisms. 2023;11(10): 2412.
Hassa, J., Tubbesing, T. J., Maus, I., Heyer, R., Benndorf, D., Effenberger, M., Henke, C., et al. (2023). Uncovering Microbiome Adaptations in a Full-Scale Biogas Plant: Insights from MAG-Centric Metagenomics and Metaproteomics. Microorganisms, 11(10), 2412. https://doi.org/10.3390/microorganisms11102412
Hassa, Julia, Tubbesing, Tom Jonas, Maus, Irena, Heyer, Robert, Benndorf, Dirk, Effenberger, Mathias, Henke, Christian, et al. 2023. “Uncovering Microbiome Adaptations in a Full-Scale Biogas Plant: Insights from MAG-Centric Metagenomics and Metaproteomics”. Microorganisms 11 (10): 2412.
Hassa, J., Tubbesing, T. J., Maus, I., Heyer, R., Benndorf, D., Effenberger, M., Henke, C., Osterholz, B., Beckstette, M., Pühler, A., et al. (2023). Uncovering Microbiome Adaptations in a Full-Scale Biogas Plant: Insights from MAG-Centric Metagenomics and Metaproteomics. Microorganisms 11:2412.
Hassa, J., et al., 2023. Uncovering Microbiome Adaptations in a Full-Scale Biogas Plant: Insights from MAG-Centric Metagenomics and Metaproteomics. Microorganisms, 11(10): 2412.
J. Hassa, et al., “Uncovering Microbiome Adaptations in a Full-Scale Biogas Plant: Insights from MAG-Centric Metagenomics and Metaproteomics”, Microorganisms, vol. 11, 2023, : 2412.
Hassa, J., Tubbesing, T.J., Maus, I., Heyer, R., Benndorf, D., Effenberger, M., Henke, C., Osterholz, B., Beckstette, M., Pühler, A., Sczyrba, A., Schlüter, A.: Uncovering Microbiome Adaptations in a Full-Scale Biogas Plant: Insights from MAG-Centric Metagenomics and Metaproteomics. Microorganisms. 11, : 2412 (2023).
Hassa, Julia, Tubbesing, Tom Jonas, Maus, Irena, Heyer, Robert, Benndorf, Dirk, Effenberger, Mathias, Henke, Christian, Osterholz, Benedikt, Beckstette, Michael, Pühler, Alfred, Sczyrba, Alexander, and Schlüter, Andreas. “Uncovering Microbiome Adaptations in a Full-Scale Biogas Plant: Insights from MAG-Centric Metagenomics and Metaproteomics”. Microorganisms 11.10 (2023): 2412.
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