Taxonomic composition and gene content of a methane-producing microbial community isolated from a biogas reactor

Krause L, Diaz NN, Edwards RA, Gartemann K-H, Krömeke H, Neuweger H, Pühler A, Runte KJ, Schlüter A, Stoye J, Szczepanowski R, et al. (2008)
JOURNAL OF BIOTECHNOLOGY 136(1-2): 91-101.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
A total community DNA sample from an agricultural biogas reactor continuously fed with maize silage, green rye, and small proportions of chicken manure has recently been sequenced using massively parallel pyrosequencing. In this Study, the sample was computationally characterized without a prior assembly step, providing quantitative insights into the taxonomic composition and gene content of the underlying microbial community. Clostridiales from the phylum Firmicutes is the most prevalent phylogenetic order, Methanomicrobiales are dominant among methanogenic archaea. An analysis of Operational Taxonomic Units (OTUs) revealed that the entire microbial community is only partially covered by the sequenced sample, despite that estimates suggest only a moderate overall diversity of the community. Furthermore, the results strongly indicate that archaea related to the genus Methanoculleus, using CO2 as electron acceptor and H-2 as electron donor, are the main producers of methane in the analyzed biogas reactor sample. A phylogenetic analysis of glycosyl hydrolase protein families suggests that Clostridia play an important role in the digestion of polysaccharides and oligosaccharides. Finally, the results unveiled that most of the organisms constituting the sample are still unexplored. (C) 2008 Elsevier B.V. All rights reserved.
Stichworte
pyrosequencing; maize silage; environmental sample; methane production; biogas reactor; biofuel; metagenome; anaerobic food chain
Erscheinungsjahr
2008
Zeitschriftentitel
JOURNAL OF BIOTECHNOLOGY
Band
136
Ausgabe
1-2
Seite(n)
91-101
ISSN
0168-1656
Page URI
https://pub.uni-bielefeld.de/record/1586009

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Krause L, Diaz NN, Edwards RA, et al. Taxonomic composition and gene content of a methane-producing microbial community isolated from a biogas reactor. JOURNAL OF BIOTECHNOLOGY. 2008;136(1-2):91-101.
Krause, L., Diaz, N. N., Edwards, R. A., Gartemann, K. - H., Krömeke, H., Neuweger, H., Pühler, A., et al. (2008). Taxonomic composition and gene content of a methane-producing microbial community isolated from a biogas reactor. JOURNAL OF BIOTECHNOLOGY, 136(1-2), 91-101. doi:10.1016/j.jbiotec.2008.06.003
Krause, L., Diaz, N. N., Edwards, R. A., Gartemann, K. - H., Krömeke, H., Neuweger, H., Pühler, A., Runte, K. J., Schlüter, A., Stoye, J., et al. (2008). Taxonomic composition and gene content of a methane-producing microbial community isolated from a biogas reactor. JOURNAL OF BIOTECHNOLOGY 136, 91-101.
Krause, L., et al., 2008. Taxonomic composition and gene content of a methane-producing microbial community isolated from a biogas reactor. JOURNAL OF BIOTECHNOLOGY, 136(1-2), p 91-101.
L. Krause, et al., “Taxonomic composition and gene content of a methane-producing microbial community isolated from a biogas reactor”, JOURNAL OF BIOTECHNOLOGY, vol. 136, 2008, pp. 91-101.
Krause, L., Diaz, N.N., Edwards, R.A., Gartemann, K.-H., Krömeke, H., Neuweger, H., Pühler, A., Runte, K.J., Schlüter, A., Stoye, J., Szczepanowski, R., Tauch, A., Goesmann, A.: Taxonomic composition and gene content of a methane-producing microbial community isolated from a biogas reactor. JOURNAL OF BIOTECHNOLOGY. 136, 91-101 (2008).
Krause, Lutz, Diaz, Naryttza N., Edwards, Robert A., Gartemann, Karl-Heinz, Krömeke, Holger, Neuweger, Heiko, Pühler, Alfred, Runte, Kai J., Schlüter, Andreas, Stoye, Jens, Szczepanowski, Rafael, Tauch, Andreas, and Goesmann, Alexander. “Taxonomic composition and gene content of a methane-producing microbial community isolated from a biogas reactor”. JOURNAL OF BIOTECHNOLOGY 136.1-2 (2008): 91-101.

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