Genome Analyses of the Less Aggressive Rhizoctonia solani AG1-IB Isolates 1/2/21 and O8/2 Compared to the Reference AG1-IB Isolate 7/3/14

Wibberg D, Genzel F, Verwaaijen B, Blom J, Rupp O, Goesmann A, Zrenner R, Grosch R, Pühler A, Schlüter A (2021)
Journal of Fungi 7(10): 832.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Rhizoctonia solani AG1-IB of the phylum Basidiomycota is known as phytopathogenic fungus affecting various economically important crops, such as bean, rice, soybean, figs, cabbage and lettuce. The isolates 1/2/21 and O8/2 of the anastomosis group AG1-IB originating from lettuce plants with bottom rot symptoms represent two less aggressive R. solani isolates, as confirmed in a pathogenicity test on lettuce. They were deeply sequenced on the Illumina MiSeq system applying the mate-pair and paired-end mode to establish their genome sequences. Assemblies of obtained sequences resulted in 2092 and 1492 scaffolds, respectively, for isolate 1/2/21 and O8/2, amounting to a size of approximately 43 Mb for each isolate. Gene prediction by applying AUGUSTUS (v. 3.2.1.) yielded 12,827 and 12,973 identified genes, respectively. Based on automatic functional annotation, genes potentially encoding cellulases and enzymes involved in secondary metabolite synthesis were identified in the AG1-IB genomes. The annotated genome sequences of the less aggressive AG1-IB isolates were compared with the isolate 7/3/14, which is highly aggressive on lettuce and other vegetable crops such as bean, cabbage and carrot. This analysis revealed the first insights into core genes of AG1-IB isolates and unique determinants of each genome that may explain the different aggressiveness levels of the strains.
Stichworte
ultrafast sequencing; AUGUSTUS; GenDBE; EDGAR
Erscheinungsjahr
2021
Zeitschriftentitel
Journal of Fungi
Band
7
Ausgabe
10
Art.-Nr.
832
eISSN
2309-608X
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Universität Bielefeld gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2957996

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Wibberg D, Genzel F, Verwaaijen B, et al. Genome Analyses of the Less Aggressive Rhizoctonia solani AG1-IB Isolates 1/2/21 and O8/2 Compared to the Reference AG1-IB Isolate 7/3/14. Journal of Fungi. 2021;7(10): 832.
Wibberg, D., Genzel, F., Verwaaijen, B., Blom, J., Rupp, O., Goesmann, A., Zrenner, R., et al. (2021). Genome Analyses of the Less Aggressive Rhizoctonia solani AG1-IB Isolates 1/2/21 and O8/2 Compared to the Reference AG1-IB Isolate 7/3/14. Journal of Fungi, 7(10), 832. https://doi.org/10.3390/jof7100832
Wibberg, D., Genzel, F., Verwaaijen, B., Blom, J., Rupp, O., Goesmann, A., Zrenner, R., Grosch, R., Pühler, A., and Schlüter, A. (2021). Genome Analyses of the Less Aggressive Rhizoctonia solani AG1-IB Isolates 1/2/21 and O8/2 Compared to the Reference AG1-IB Isolate 7/3/14. Journal of Fungi 7:832.
Wibberg, D., et al., 2021. Genome Analyses of the Less Aggressive Rhizoctonia solani AG1-IB Isolates 1/2/21 and O8/2 Compared to the Reference AG1-IB Isolate 7/3/14. Journal of Fungi, 7(10): 832.
D. Wibberg, et al., “Genome Analyses of the Less Aggressive Rhizoctonia solani AG1-IB Isolates 1/2/21 and O8/2 Compared to the Reference AG1-IB Isolate 7/3/14”, Journal of Fungi, vol. 7, 2021, : 832.
Wibberg, D., Genzel, F., Verwaaijen, B., Blom, J., Rupp, O., Goesmann, A., Zrenner, R., Grosch, R., Pühler, A., Schlüter, A.: Genome Analyses of the Less Aggressive Rhizoctonia solani AG1-IB Isolates 1/2/21 and O8/2 Compared to the Reference AG1-IB Isolate 7/3/14. Journal of Fungi. 7, : 832 (2021).
Wibberg, Daniel, Genzel, Franziska, Verwaaijen, Bart, Blom, Jochen, Rupp, Oliver, Goesmann, Alexander, Zrenner, Rita, Grosch, Rita, Pühler, Alfred, and Schlüter, Andreas. “Genome Analyses of the Less Aggressive Rhizoctonia solani AG1-IB Isolates 1/2/21 and O8/2 Compared to the Reference AG1-IB Isolate 7/3/14”. Journal of Fungi 7.10 (2021): 832.
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2021-10-08T09:22:47Z
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