High-quality genome sequence of Pichia pastoris CBS7435

Küberl, Andreas; Schneider, Jessica; Thallinger, Gerhard G.; Anderl, Ingund; Wibberg, Daniel; Hajek, Tanja; Jaenicke, Sebastian; Brinkrolf, Karina; Goesmann, Alexander; Szczepanowski, Rafael; Pühler, Alfred; Schwab, Helmut; Glieder, Anton; Pichler, Harald

High-quality genome sequence of Pichia pastoris CBS7435

Küberl A, Schneider J, Thallinger GG, Anderl I, Wibberg D, Hajek T, Jaenicke S, Brinkrolf K, Goesmann A, Szczepanowski R, Pühler A, et al. (2011)
Journal of Biotechnology 154(4): 312-320.

Zeitschriftenaufsatz | Veröffentlicht | Englisch

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DOI

https://doi.org/10.1016/j.jbiotec.2011.04.014

Autor*in

Küberl, Andreas; Schneider, Jessica^UniBi; Thallinger, Gerhard G.; Anderl, Ingund; Wibberg, Daniel^UniBi; Hajek, Tanja; Jaenicke, Sebastian^UniBi; Brinkrolf, Karina^UniBi; Goesmann, Alexander^UniBi ; Szczepanowski, Rafael^UniBi; Pühler, Alfred^UniBi ; Schwab, Helmut
Alle

Einrichtung

Centrum für Biotechnologie > Technologieplattformen > Bioinformatics Resource Facility
Centrum für Biotechnologie > Institut für Bioinformatik
Centrum für Biotechnologie > Institut für Genomforschung und Systembiologie
Centrum für Biotechnologie > Arbeitsgruppe A. Goesmann
Technische Fakultät > Computational Genomics
Centrum für Biotechnologie > Arbeitsgruppe A. Pühler
Centrum für Biotechnologie > Graduate Center > Graduate Cluster Industrial Biotechnology

Abstract / Bemerkung

The methylotrophic yeast Pichia pastoris (Komagataella phaffii) CBS7435 is the parental strain of commonly used P. pastoris recombinant protein production hosts making it well suited for improving the understanding of associated genomic features. Here, we present a 9.35Mbp high-quality genome sequence of P. pastoris CBS7435 established by a combination of 454 and Illumina sequencing. An automatic annotation of the genome sequence yielded 5007 protein-coding genes, 124 tRNAs and 29 rRNAs. Moreover, we report the complete DNA sequence of the first mitochondrial genome of a methylotrophic yeast. Fifteen genes encoding proteins, 2 rRNA and 25 tRNA loci were identified on the 35.7kbp circular, mitochondrial DNA. Furthermore, the architecture of the putative alpha mating factor protein of P. pastoris CBS7435 turned out to be more complex than the corresponding protein of Saccharomyces cerevisiae. Copyright 2011 Elsevier B.V. All rights reserved.

Erscheinungsjahr

2011

Zeitschriftentitel

Journal of Biotechnology

Band

154

Ausgabe

Seite(n)

312-320

ISSN

0168-1656

Page URI

https://pub.uni-bielefeld.de/record/2317371

Zitieren

Küberl A, Schneider J, Thallinger GG, et al. High-quality genome sequence of Pichia pastoris CBS7435. Journal of Biotechnology. 2011;154(4):312-320.

Küberl, A., Schneider, J., Thallinger, G. G., Anderl, I., Wibberg, D., Hajek, T., Jaenicke, S., et al. (2011). High-quality genome sequence of Pichia pastoris CBS7435. Journal of Biotechnology, 154(4), 312-320. https://doi.org/10.1016/j.jbiotec.2011.04.014

Küberl, Andreas, Schneider, Jessica, Thallinger, Gerhard G., Anderl, Ingund, Wibberg, Daniel, Hajek, Tanja, Jaenicke, Sebastian, et al. 2011. “High-quality genome sequence of Pichia pastoris CBS7435”. Journal of Biotechnology 154 (4): 312-320.

Küberl, A., Schneider, J., Thallinger, G. G., Anderl, I., Wibberg, D., Hajek, T., Jaenicke, S., Brinkrolf, K., Goesmann, A., Szczepanowski, R., et al. (2011). High-quality genome sequence of Pichia pastoris CBS7435. Journal of Biotechnology 154, 312-320.

Küberl, A., et al., 2011. High-quality genome sequence of Pichia pastoris CBS7435. Journal of Biotechnology, 154(4), p 312-320.

A. Küberl, et al., “High-quality genome sequence of Pichia pastoris CBS7435”, Journal of Biotechnology, vol. 154, 2011, pp. 312-320.

Küberl, A., Schneider, J., Thallinger, G.G., Anderl, I., Wibberg, D., Hajek, T., Jaenicke, S., Brinkrolf, K., Goesmann, A., Szczepanowski, R., Pühler, A., Schwab, H., Glieder, A., Pichler, H.: High-quality genome sequence of Pichia pastoris CBS7435. Journal of Biotechnology. 154, 312-320 (2011).

Küberl, Andreas, Schneider, Jessica, Thallinger, Gerhard G., Anderl, Ingund, Wibberg, Daniel, Hajek, Tanja, Jaenicke, Sebastian, Brinkrolf, Karina, Goesmann, Alexander, Szczepanowski, Rafael, Pühler, Alfred, Schwab, Helmut, Glieder, Anton, and Pichler, Harald. “High-quality genome sequence of Pichia pastoris CBS7435”. Journal of Biotechnology 154.4 (2011): 312-320.

Daten bereitgestellt von European Bioinformatics Institute (EBI)

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