Ultrafast pyrosequencing of Corynebacterium kroppenstedtii DSM44385 revealed insights into the physiology of a lipophilic corynebacterium that lacks mycolic acids

Tauch A, Schneider J, Szczepanowski R, Tilker A, Viehöver P, Gartemann K-H, Arnold W, Blom J, Brinkrolf K, Brune I, Goetker S, et al. (2008)
Journal of Biotechnology 136(1-2): 22-30.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Corynebacterium kroppenstedtii is a lipophilic corynebacterial species that lacks in the cell envelope the characteristic alpha-alkyl-beta-hyclroxy long-chain fatty acids, designated mycolic acids. We report here the bioinformatic analysis of genome data obtained by pyrosequencing of the type strain C kroppenstedtii DSM44385 that was initially isolated from human Sputum. A single run with the Genome Sequencer FLX system revealed 560,248 Shotgun reads with 110,018,974 detected bases that were assembled into a contiguous genomic sequence with a total size of 2,446,804 bp. Automatic annotation of the complete genome sequence resulted in the prediction of 2122 coding sequences, of which 29% were considered as specific for C. kroppenstedtii when compared with predicted proteins from hitherto sequenced pathogenic corynebacteria. This Comparative content analysis of the genome data revealed a large repertoire of genes involved in sugar Uptake and central carbohydrate metabolism and the presence of the mevalonate route for isoprenoid biosynthesis. The lack of mycolic acids and the lipophilic lifestyle of C. kroppenstedtii are apparently caused by gene loss, including a condensase gene cluster, a mycolate reductase gene, and a microbial type I fatty acid synthase gene. A complete beta-oxidation pathway involved in the degradation of fatty acids is present in the genome. Evaluation of the genomic data indicated that lipophilism is the dominant feature involved in pathogenicity of C. kroppenstedtii. (C) 2008 Elsevier B.V. All rights reserved.
Stichworte
isoprenoid biosynthesis; beta-oxidation; mycolic acid biosynthesis; lipophilism; mevalonate pathway; Corynebacterium kroppenstentii; biotin biosynthesis; pyrosequencing
Erscheinungsjahr
2008
Zeitschriftentitel
Journal of Biotechnology
Band
136
Ausgabe
1-2
Seite(n)
22-30
ISSN
0168-1656
Page URI
https://pub.uni-bielefeld.de/record/1585939

Zitieren

Tauch A, Schneider J, Szczepanowski R, et al. Ultrafast pyrosequencing of Corynebacterium kroppenstedtii DSM44385 revealed insights into the physiology of a lipophilic corynebacterium that lacks mycolic acids. Journal of Biotechnology. 2008;136(1-2):22-30.
Tauch, A., Schneider, J., Szczepanowski, R., Tilker, A., Viehöver, P., Gartemann, K. - H., Arnold, W., et al. (2008). Ultrafast pyrosequencing of Corynebacterium kroppenstedtii DSM44385 revealed insights into the physiology of a lipophilic corynebacterium that lacks mycolic acids. Journal of Biotechnology, 136(1-2), 22-30. doi:10.1016/j.jbiotec.2008.03.004
Tauch, A., Schneider, J., Szczepanowski, R., Tilker, A., Viehöver, P., Gartemann, K. - H., Arnold, W., Blom, J., Brinkrolf, K., Brune, I., et al. (2008). Ultrafast pyrosequencing of Corynebacterium kroppenstedtii DSM44385 revealed insights into the physiology of a lipophilic corynebacterium that lacks mycolic acids. Journal of Biotechnology 136, 22-30.
Tauch, A., et al., 2008. Ultrafast pyrosequencing of Corynebacterium kroppenstedtii DSM44385 revealed insights into the physiology of a lipophilic corynebacterium that lacks mycolic acids. Journal of Biotechnology, 136(1-2), p 22-30.
A. Tauch, et al., “Ultrafast pyrosequencing of Corynebacterium kroppenstedtii DSM44385 revealed insights into the physiology of a lipophilic corynebacterium that lacks mycolic acids”, Journal of Biotechnology, vol. 136, 2008, pp. 22-30.
Tauch, A., Schneider, J., Szczepanowski, R., Tilker, A., Viehöver, P., Gartemann, K.-H., Arnold, W., Blom, J., Brinkrolf, K., Brune, I., Goetker, S., Weisshaar, B., Goesmann, A., Droege, M., Pühler, A.: Ultrafast pyrosequencing of Corynebacterium kroppenstedtii DSM44385 revealed insights into the physiology of a lipophilic corynebacterium that lacks mycolic acids. Journal of Biotechnology. 136, 22-30 (2008).
Tauch, Andreas, Schneider, Jessica, Szczepanowski, Rafael, Tilker, Alexandra, Viehöver, Prisca, Gartemann, Karl-Heinz, Arnold, Walter, Blom, Jochen, Brinkrolf, Karina, Brune, Iris, Goetker, Susanne, Weisshaar, Bernd, Goesmann, Alexander, Droege, Marcus, and Pühler, Alfred. “Ultrafast pyrosequencing of Corynebacterium kroppenstedtii DSM44385 revealed insights into the physiology of a lipophilic corynebacterium that lacks mycolic acids”. Journal of Biotechnology 136.1-2 (2008): 22-30.

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