Improving the genome annotation of the acarbose producer Actinoplanes sp. SE50/110 by sequencing enriched 5'-ends of primary transcripts

Schwientek P, Neshat A, Kalinowski J, Klein A, Rückert C, Schneiker-Bekel S, Wendler S, Stoye J, Pühler A (2014)
Journal of Biotechnology 190: 85-95.

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DOI
Autor*in
Schwientek, PatrickUniBi; Neshat, ArminUniBi; Kalinowski, JörnUniBi; Klein, Andreas; Rückert, ChristianUniBi ; Schneiker-Bekel, SusanneUniBi; Wendler, SergejUniBi; Stoye, JensUniBi ; Pühler, AlfredUniBi
Einrichtung
Centrum für Biotechnologie > Arbeitsgruppe A. Pühler
Centrum für Biotechnologie > Graduate Center > Graduate Cluster Industrial Biotechnology
Centrum für Biotechnologie > Institut für Genomforschung und Systembiologie
Centrum für Biotechnologie > Arbeitsgruppe J. Kalinowski
Centrum für Biotechnologie > Arbeitsgruppe J. Stoye
Centrum für Biotechnologie > Institut für Bioinformatik
Technische Fakultät > AG Genominformatik
Abstract / Bemerkung
: Actinoplanes sp. SE50/110 is the producer of the alpha-glucosidase inhibitor acarbose, which is an economically relevant and potent drug in the treatment of type-2 diabetes mellitus. In this study, we present the detection of transcription start sites on this genome by sequencing enriched 5'-ends of primary transcripts. Altogether, 1,427 putative transcription start sites were initially identified. With help of the annotated genome sequence, 661 transcription start sites were found to belong to the leader region of protein-coding genes with the surprising result that roughly 20% of these genes rank among the class of leaderless transcripts. Next, conserved promoter motifs were identified for protein-coding genes with and without leader sequences. The mapped transcription start sites were finally used to improve the annotation of the Actinoplanes sp. SE50/110 genome sequence. Concerning protein-coding genes, 41 translation start sites were corrected and 9 novel protein-coding genes could be identified. In addition to this, 122 previously undetermined non-coding RNA (ncRNA) genes of Actinoplanes sp. SE50/110 were defined. Focusing on antisense transcription start sites located within coding genes or their leader sequences, it was discovered that 96 of those ncRNA genes belong to the class of antisense RNA (asRNA) genes. The remaining 26 ncRNA genes were found outside of known protein-coding genes. Four chosen examples of prominent ncRNA genes, namely the transfer messenger RNA gene ssrA, the ribonuclease P class A RNA gene rnpB, the cobalamin riboswitch RNA gene cobRS, and the selenocysteine-specific tRNA gene selC, are presented in more detail. This study demonstrates that sequencing of enriched 5'-ends of primary transcripts and the identification of transcription start sites are valuable tools for advanced genome annotation of Actinoplanes sp. SE50/110 and most probably also for other bacteria.
Erscheinungsjahr
2014
Zeitschriftentitel
Journal of Biotechnology
Band
190
Seite(n)
85-95
ISSN
0168-1656
Page URI
https://pub.uni-bielefeld.de/record/2665038

Zitieren

Schwientek P, Neshat A, Kalinowski J, et al. Improving the genome annotation of the acarbose producer Actinoplanes sp. SE50/110 by sequencing enriched 5'-ends of primary transcripts. Journal of Biotechnology. 2014;190:85-95.
Schwientek, P., Neshat, A., Kalinowski, J., Klein, A., Rückert, C., Schneiker-Bekel, S., Wendler, S., et al. (2014). Improving the genome annotation of the acarbose producer Actinoplanes sp. SE50/110 by sequencing enriched 5'-ends of primary transcripts. Journal of Biotechnology, 190, 85-95. doi:10.1016/j.jbiotec.2014.03.013
Schwientek, Patrick, Neshat, Armin, Kalinowski, Jörn, Klein, Andreas, Rückert, Christian, Schneiker-Bekel, Susanne, Wendler, Sergej, Stoye, Jens, and Pühler, Alfred. 2014. “Improving the genome annotation of the acarbose producer Actinoplanes sp. SE50/110 by sequencing enriched 5'-ends of primary transcripts”. Journal of Biotechnology 190: 85-95.
Schwientek, P., Neshat, A., Kalinowski, J., Klein, A., Rückert, C., Schneiker-Bekel, S., Wendler, S., Stoye, J., and Pühler, A. (2014). Improving the genome annotation of the acarbose producer Actinoplanes sp. SE50/110 by sequencing enriched 5'-ends of primary transcripts. Journal of Biotechnology 190, 85-95.
Schwientek, P., et al., 2014. Improving the genome annotation of the acarbose producer Actinoplanes sp. SE50/110 by sequencing enriched 5'-ends of primary transcripts. Journal of Biotechnology, 190, p 85-95.
P. Schwientek, et al., “Improving the genome annotation of the acarbose producer Actinoplanes sp. SE50/110 by sequencing enriched 5'-ends of primary transcripts”, Journal of Biotechnology, vol. 190, 2014, pp. 85-95.
Schwientek, P., Neshat, A., Kalinowski, J., Klein, A., Rückert, C., Schneiker-Bekel, S., Wendler, S., Stoye, J., Pühler, A.: Improving the genome annotation of the acarbose producer Actinoplanes sp. SE50/110 by sequencing enriched 5'-ends of primary transcripts. Journal of Biotechnology. 190, 85-95 (2014).
Schwientek, Patrick, Neshat, Armin, Kalinowski, Jörn, Klein, Andreas, Rückert, Christian, Schneiker-Bekel, Susanne, Wendler, Sergej, Stoye, Jens, and Pühler, Alfred. “Improving the genome annotation of the acarbose producer Actinoplanes sp. SE50/110 by sequencing enriched 5'-ends of primary transcripts”. Journal of Biotechnology 190 (2014): 85-95.

7 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Genome improvement of the acarbose producer Actinoplanes sp. SE50/110 and annotation refinement based on RNA-seq analysis.
Wolf T, Schneiker-Bekel S, Neshat A, Ortseifen V, Wibberg D, Zemke T, Pühler A, Kalinowski J., J Biotechnol 251(), 2017
PMID: 28427920
Detailed transcriptome analysis of the plant growth promoting Paenibacillus riograndensis SBR5 by using RNA-seq technology.
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Zhao Q, Xie H, Peng Y, Wang X, Bai L., Synth Syst Biotechnol 2(4), 2017
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Comparative proteome analysis of Actinoplanes sp. SE50/110 grown with maltose or glucose shows minor differences for acarbose biosynthesis proteins but major differences for saccharide transporters.
Wendler S, Otto A, Ortseifen V, Bonn F, Neshat A, Schneiker-Bekel S, Wolf T, Zemke T, Wehmeier UF, Hecker M, Kalinowski J, Becher D, Pühler A., J Proteomics 131(), 2016
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