Chinese hamster genome sequenced from sorted chromosomes

Brinkrolf K, Rupp O, Laux H, Kollin F, Ernst W, Linke B, Kofler R, Romand S, Hesse F, Budach WE, Galosy S, et al. (2013)
Nature Biotechnology 31(8): 694-695.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Brinkrolf, KarinaUniBi; Rupp, OliverUniBi; Laux, Holger; Kollin, FlorianUniBi; Ernst, Wolfgang; Linke, BurkhardUniBi; Kofler, Rudolf; Romand, Sandrine; Hesse, Friedemann; Budach, Wolfgang E; Galosy, Sybille; Müller, Dethardt
Nature Biotechnology
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Brinkrolf K, Rupp O, Laux H, et al. Chinese hamster genome sequenced from sorted chromosomes. Nature Biotechnology. 2013;31(8):694-695.
Brinkrolf, K., Rupp, O., Laux, H., Kollin, F., Ernst, W., Linke, B., Kofler, R., et al. (2013). Chinese hamster genome sequenced from sorted chromosomes. Nature Biotechnology, 31(8), 694-695. doi:10.1038/nbt.2645
Brinkrolf, K., Rupp, O., Laux, H., Kollin, F., Ernst, W., Linke, B., Kofler, R., Romand, S., Hesse, F., Budach, W. E., et al. (2013). Chinese hamster genome sequenced from sorted chromosomes. Nature Biotechnology 31, 694-695.
Brinkrolf, K., et al., 2013. Chinese hamster genome sequenced from sorted chromosomes. Nature Biotechnology, 31(8), p 694-695.
K. Brinkrolf, et al., “Chinese hamster genome sequenced from sorted chromosomes”, Nature Biotechnology, vol. 31, 2013, pp. 694-695.
Brinkrolf, K., Rupp, O., Laux, H., Kollin, F., Ernst, W., Linke, B., Kofler, R., Romand, S., Hesse, F., Budach, W.E., Galosy, S., Müller, D., Noll, T., Wienberg, J., Jostock, T., Leonard, M., Grillari, J., Tauch, A., Goesmann, A., Helk, B., Mott, J.E., Pühler, A., Borth, N.: Chinese hamster genome sequenced from sorted chromosomes. Nature Biotechnology. 31, 694-695 (2013).
Brinkrolf, Karina, Rupp, Oliver, Laux, Holger, Kollin, Florian, Ernst, Wolfgang, Linke, Burkhard, Kofler, Rudolf, Romand, Sandrine, Hesse, Friedemann, Budach, Wolfgang E, Galosy, Sybille, Müller, Dethardt, Noll, Thomas, Wienberg, Johannes, Jostock, Thomas, Leonard, Mark, Grillari, Johannes, Tauch, Andreas, Goesmann, Alexander, Helk, Bernhard, Mott, John E, Pühler, Alfred, and Borth, Nicole. “Chinese hamster genome sequenced from sorted chromosomes”. Nature Biotechnology 31.8 (2013): 694-695.

68 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Epigenetic regulation of gene expression in Chinese Hamster Ovary cells in response to the changing environment of a batch culture.
Hernandez I, Dhiman H, Klanert G, Jadhav V, Auer N, Hanscho M, Baumann M, Esteve-Codina A, Dabad M, Gómez J, Alioto T, Merkel A, Raineri E, Heath S, Rico D, Borth N., Biotechnol Bioeng 116(3), 2019
PMID: 30512195
CRISPR/CAS9 targeted CAPTURE of mammalian genomic regions for characterization by NGS.
Slesarev A, Viswanathan L, Tang Y, Borgschulte T, Achtien K, Razafsky D, Onions D, Chang A, Cote C., Sci Rep 9(1), 2019
PMID: 30837529
A cross-species whole genome siRNA screen in suspension-cultured Chinese hamster ovary cells identifies novel engineering targets.
Klanert G, Fernandez DJ, Weinguny M, Eisenhut P, Bühler E, Melcher M, Titus SA, Diendorfer AB, Gludovacz E, Jadhav V, Xiao S, Stern B, Lal M, Shiloach J, Borth N., Sci Rep 9(1), 2019
PMID: 31213643
Transcriptome-Based Identification of the Optimal Reference CHO Genes for Normalisation of qPCR Data.
Brown AJ, Gibson S, Hatton D, James DC., Biotechnol J 13(1), 2018
PMID: 28731643
Enhanced Genome Editing Tools For Multi-Gene Deletion Knock-Out Approaches Using Paired CRISPR sgRNAs in CHO Cells.
Schmieder V, Bydlinski N, Strasser R, Baumann M, Kildegaard HF, Jadhav V, Borth N., Biotechnol J 13(3), 2018
PMID: 28976642
CHO-Omics Review: The Impact of Current and Emerging Technologies on Chinese Hamster Ovary Based Bioproduction.
Stolfa G, Smonskey MT, Boniface R, Hachmann AB, Gulde P, Joshi AD, Pierce AP, Jacobia SJ, Campbell A., Biotechnol J 13(3), 2018
PMID: 29072373
Karyotype variation of CHO host cell lines over time in culture characterized by chromosome counting and chromosome painting.
Vcelar S, Jadhav V, Melcher M, Auer N, Hrdina A, Sagmeister R, Heffner K, Puklowski A, Betenbaugh M, Wenger T, Leisch F, Baumann M, Borth N., Biotechnol Bioeng 115(1), 2018
PMID: 28921524
RNA-Seq Highlights High Clonal Variation in Monoclonal Antibody Producing CHO Cells.
Orellana CA, Marcellin E, Palfreyman RW, Munro TP, Gray PP, Nielsen LK., Biotechnol J 13(3), 2018
PMID: 29316330
Glycoengineering in CHO Cells: Advances in Systems Biology.
Tejwani V, Andersen MR, Nam JH, Sharfstein ST., Biotechnol J 13(3), 2018
PMID: 29316325
An adaptable chromosome preparation methodology for use in invertebrate research organisms.
Guo L, Accorsi A, He S, Guerrero-Hernández C, Sivagnanam S, McKinney S, Gibson M, Sánchez Alvarado A., BMC Biol 16(1), 2018
PMID: 29482548
A reference genome of the Chinese hamster based on a hybrid assembly strategy.
Rupp O, MacDonald ML, Li S, Dhiman H, Polson S, Griep S, Heffner K, Hernandez I, Brinkrolf K, Jadhav V, Samoudi M, Hao H, Kingham B, Goesmann A, Betenbaugh MJ, Lewis NE, Borth N, Lee KH., Biotechnol Bioeng 115(8), 2018
PMID: 29704459
CRISPR-Based Targeted Epigenetic Editing Enables Gene Expression Modulation of the Silenced Beta-Galactoside Alpha-2,6-Sialyltransferase 1 in CHO Cells.
Marx N, Grünwald-Gruber C, Bydlinski N, Dhiman H, Ngoc Nguyen L, Klanert G, Borth N., Biotechnol J 13(10), 2018
PMID: 29802757
Degradation of recombinant proteins by Chinese hamster ovary host cell proteases is prevented by matriptase-1 knockout.
Laux H, Romand S, Nuciforo S, Farady CJ, Tapparel J, Buechmann-Moeller S, Sommer B, Oakeley EJ, Bodendorf U., Biotechnol Bioeng 115(10), 2018
PMID: 29777593
CHOmine: an integrated data warehouse for CHO systems biology and modeling.
Gerstl MP, Hanscho M, Ruckerbauer DE, Zanghellini J, Borth N., Database (Oxford) 2017(), 2017
PMID: 28605771
Ribosome profiling-guided depletion of an mRNA increases cell growth rate and protein secretion.
Kallehauge TB, Li S, Pedersen LE, Ha TK, Ley D, Andersen MR, Kildegaard HF, Lee GM, Lewis NE., Sci Rep 7(), 2017
PMID: 28091612
Regulation of miR-29b-1/a transcription and identification of target mRNAs in CHO-K1 cells.
Muluhngwi P, Richardson K, Napier J, Rouchka EC, Mott JL, Klinge CM., Mol Cell Endocrinol 444(), 2017
PMID: 28137615
Ultra-deep next generation mitochondrial genome sequencing reveals widespread heteroplasmy in Chinese hamster ovary cells.
Kelly PS, Clarke C, Costello A, Monger C, Meiller J, Dhiman H, Borth N, Betenbaugh MJ, Clynes M, Barron N., Metab Eng 41(), 2017
PMID: 28188893
Y and W Chromosome Assemblies: Approaches and Discoveries.
Tomaszkiewicz M, Medvedev P, Makova KD., Trends Genet 33(4), 2017
PMID: 28236503
Network reconstruction of the mouse secretory pathway applied on CHO cell transcriptome data.
Lund AM, Kaas CS, Brandl J, Pedersen LE, Kildegaard HF, Kristensen C, Andersen MR., BMC Syst Biol 11(1), 2017
PMID: 28298216
Therapeutic glycoprotein production in mammalian cells.
Lalonde ME, Durocher Y., J Biotechnol 251(), 2017
PMID: 28465209
Mammalian Systems Biotechnology Reveals Global Cellular Adaptations in a Recombinant CHO Cell Line.
Yusufi FNK, Lakshmanan M, Ho YS, Loo BLW, Ariyaratne P, Yang Y, Ng SK, Tan TRM, Yeo HC, Lim HL, Ng SW, Hiu AP, Chow CP, Wan C, Chen S, Teo G, Song G, Chin JX, Ruan X, Sung KWK, Hu WS, Yap MGS, Bardor M, Nagarajan N, Lee DY., Cell Syst 4(5), 2017
PMID: 28544881
Preselection of recombinant gene integration sites enabling high transcription rates in CHO cells using alternate start codons and recombinase mediated cassette exchange.
Baumann M, Gludovacz E, Sealover N, Bahr S, George H, Lin N, Kayser K, Borth N., Biotechnol Bioeng 114(11), 2017
PMID: 28734047
Deletion of a telomeric region on chromosome 8 correlates with higher productivity and stability of CHO cell lines.
Ritter A, Voedisch B, Wienberg J, Wilms B, Geisse S, Jostock T, Laux H., Biotechnol Bioeng 113(5), 2016
PMID: 26523402
Improving expression of recombinant human IGF-1 using IGF-1R knockout CHO cell lines.
Romand S, Jostock T, Fornaro M, Schmidt J, Ritter A, Wilms B, Laux H., Biotechnol Bioeng 113(5), 2016
PMID: 26523469
A signature of 12 microRNAs is robustly associated with growth rate in a variety of CHO cell lines.
Klanert G, Jadhav V, Shanmukam V, Diendorfer A, Karbiener M, Scheideler M, Bort JH, Grillari J, Hackl M, Borth N., J Biotechnol 235(), 2016
PMID: 26993211
Comprehensive genome and epigenome characterization of CHO cells in response to evolutionary pressures and over time.
Feichtinger J, Hernández I, Fischer C, Hanscho M, Auer N, Hackl M, Jadhav V, Baumann M, Krempl PM, Schmidl C, Farlik M, Schuster M, Merkel A, Sommer A, Heath S, Rico D, Bock C, Thallinger GG, Borth N., Biotechnol Bioeng 113(10), 2016
PMID: 27072894
Disruption of the gene C12orf35 leads to increased productivities in recombinant CHO cell lines.
Ritter A, Rauschert T, Oertli M, Piehlmaier D, Mantas P, Kuntzelmann G, Lageyre N, Brannetti B, Voedisch B, Geisse S, Jostock T, Laux H., Biotechnol Bioeng 113(11), 2016
PMID: 27183150
Omics insights into production-scale bioreactors.
Sharfstein ST., Biotechnol J 11(9), 2016
PMID: 27351837
Evolutionary dynamics of Anolis sex chromosomes revealed by sequencing of flow sorting-derived microchromosome-specific DNA.
Kichigin IG, Giovannotti M, Makunin AI, Ng BL, Kabilov MR, Tupikin AE, Barucchi VC, Splendiani A, Ruggeri P, Rens W, O'Brien PC, Ferguson-Smith MA, Graphodatsky AS, Trifonov VA., Mol Genet Genomics 291(5), 2016
PMID: 27431992
A hybrid approach identifies metabolic signatures of high-producers for chinese hamster ovary clone selection and process optimization.
Popp O, Müller D, Didzus K, Paul W, Lipsmeier F, Kirchner F, Niklas J, Mauch K, Beaucamp N., Biotechnol Bioeng 113(9), 2016
PMID: 26913695
Augmenting Chinese hamster genome assembly by identifying regions of high confidence.
Vishwanathan N, Bandyopadhyay AA, Fu HY, Sharma M, Johnson KC, Mudge J, Ramaraj T, Onsongo G, Silverstein KA, Jacob NM, Le H, Karypis G, Hu WS., Biotechnol J 11(9), 2016
PMID: 27374913
Contrasting origin of B chromosomes in two cervids (Siberian roe deer and grey brocket deer) unravelled by chromosome-specific DNA sequencing.
Makunin AI, Kichigin IG, Larkin DM, O'Brien PC, Ferguson-Smith MA, Yang F, Proskuryakova AA, Vorobieva NV, Chernyaeva EN, O'Brien SJ, Graphodatsky AS, Trifonov VA., BMC Genomics 17(1), 2016
PMID: 27516089
Engineering Translation in Mammalian Cell Factories to Increase Protein Yield: The Unexpected Use of Long Non-Coding SINEUP RNAs.
Zucchelli S, Patrucco L, Persichetti F, Gustincich S, Cotella D., Comput Struct Biotechnol J 14(), 2016
PMID: 27872686
A Consensus Genome-scale Reconstruction of Chinese Hamster Ovary Cell Metabolism.
Hefzi H, Ang KS, Hanscho M, Bordbar A, Ruckerbauer D, Lakshmanan M, Orellana CA, Baycin-Hizal D, Huang Y, Ley D, Martinez VS, Kyriakopoulos S, Jiménez NE, Zielinski DC, Quek LE, Wulff T, Arnsdorf J, Li S, Lee JS, Paglia G, Loira N, Spahn PN, Pedersen LE, Gutierrez JM, King ZA, Lund AM, Nagarajan H, Thomas A, Abdel-Haleem AM, Zanghellini J, Kildegaard HF, Voldborg BG, Gerdtzen ZP, Betenbaugh MJ, Palsson BO, Andersen MR, Nielsen LK, Borth N, Lee DY, Lewis NE., Cell Syst 3(5), 2016
PMID: 27883890
Sequencing technologies for animal cell culture research.
Kremkow BG, Lee KH., Biotechnol Lett 37(1), 2015
PMID: 25214225
The Genome 10K Project: a way forward.
Koepfli KP, Paten B, Genome 10K Community of Scientists, O'Brien SJ., Annu Rev Anim Biosci 3(), 2015
PMID: 25689317
Unveiling the principle of microRNA-mediated redundancy in cellular pathway regulation.
Fischer S, Handrick R, Aschrafi A, Otte K., RNA Biol 12(3), 2015
PMID: 25826657
Engineering the supply chain for protein production/secretion in yeasts and mammalian cells.
Klein T, Niklas J, Heinzle E., J Ind Microbiol Biotechnol 42(3), 2015
PMID: 25561318
The DNA methylation landscape of Chinese hamster ovary (CHO) DP-12 cells.
Wippermann A, Rupp O, Brinkrolf K, Hoffrogge R, Noll T., J Biotechnol 199(), 2015
PMID: 25701679
Sequencing the CHO DXB11 genome reveals regional variations in genomic stability and haploidy.
Kaas CS, Kristensen C, Betenbaugh MJ, Andersen MR., BMC Genomics 16(), 2015
PMID: 25887056
Annotation of additional evolutionary conserved microRNAs in CHO cells from updated genomic data.
Diendorfer AB, Hackl M, Klanert G, Jadhav V, Reithofer M, Stiefel F, Hesse F, Grillari J, Borth N., Biotechnol Bioeng 112(7), 2015
PMID: 25689160 2.0: Genome resources and website updates.
Kremkow BG, Baik JY, MacDonald ML, Lee KH., Biotechnol J 10(7), 2015
PMID: 25923813
Cell line development for biomanufacturing processes: recent advances and an outlook.
Le H, Vishwanathan N, Jacob NM, Gadgil M, Hu WS., Biotechnol Lett 37(8), 2015
PMID: 25971160
CRISPR/Cas9-mediated genome engineering of CHO cell factories: Application and perspectives.
Lee JS, Grav LM, Lewis NE, Faustrup Kildegaard H., Biotechnol J 10(7), 2015
PMID: 26058577
Towards next generation CHO cell biology: Bioinformatics methods for RNA-Seq-based expression profiling.
Monger C, Kelly PS, Gallagher C, Clynes M, Barron N, Clarke C., Biotechnol J 10(7), 2015
PMID: 26058739
Enhanced protein production by microRNA-30 family in CHO cells is mediated by the modulation of the ubiquitin pathway.
Fischer S, Mathias S, Schaz S, Emmerling VV, Buck T, Kleemann M, Hackl M, Grillari J, Aschrafi A, Handrick R, Otte K., J Biotechnol 212(), 2015
PMID: 26256096
Identification of active elementary flux modes in mitochondria using selectively permeabilized CHO cells.
Nicolae A, Wahrheit J, Nonnenmacher Y, Weyler C, Heinzle E., Metab Eng 32(), 2015
PMID: 26417715
Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells.
Hansen HG, Nilsson CN, Lund AM, Kol S, Grav LM, Lundqvist M, Rockberg J, Lee GM, Andersen MR, Kildegaard HF., Sci Rep 5(), 2015
PMID: 26657798
The role of replicates for error mitigation in next-generation sequencing.
Robasky K, Lewis NE, Church GM., Nat Rev Genet 15(1), 2014
PMID: 24322726
Construction of a public CHO cell line transcript database using versatile bioinformatics analysis pipelines.
Rupp O, Becker J, Brinkrolf K, Timmermann C, Borth N, Pühler A, Noll T, Goesmann A., PLoS One 9(1), 2014
PMID: 24427317
Analysis of microRNA transcription and post-transcriptional processing by Dicer in the context of CHO cell proliferation.
Hackl M, Jadhav V, Klanert G, Karbiener M, Scheideler M, Grillari J, Borth N., J Biotechnol 190(), 2014
PMID: 24486028
Toward product attribute control: developments from genome sequencing.
Baik JY, Lee KH., Curr Opin Biotechnol 30(), 2014
PMID: 24874795
Advancing biopharmaceutical process science through transcriptome analysis.
Vishwanathan N, Le H, Le T, Hu WS., Curr Opin Biotechnol 30(), 2014
PMID: 25014889
CHOPPI: a web tool for the analysis of immunogenicity risk from host cell proteins in CHO-based protein production.
Bailey-Kellogg C, Gutiérrez AH, Moise L, Terry F, Martin WD, De Groot AS., Biotechnol Bioeng 111(11), 2014
PMID: 24888712
Identification of microRNAs specific for high producer CHO cell lines using steady-state cultivation.
Maccani A, Hackl M, Leitner C, Steinfellner W, Graf AB, Tatto NE, Karbiener M, Scheideler M, Grillari J, Mattanovich D, Kunert R, Borth N, Grabherr R, Ernst W., Appl Microbiol Biotechnol 98(17), 2014
PMID: 25052466
Discovery of transcription start sites in the Chinese hamster genome by next-generation RNA sequencing.
Jakobi T, Brinkrolf K, Tauch A, Noll T, Stoye J, Pühler A, Goesmann A., J Biotechnol 190(), 2014
PMID: 25086342
A functional high-content miRNA screen identifies miR-30 family to boost recombinant protein production in CHO cells.
Fischer S, Buck T, Wagner A, Ehrhart C, Giancaterino J, Mang S, Schad M, Mathias S, Aschrafi A, Handrick R, Otte K., Biotechnol J 9(10), 2014
PMID: 25061012
Adaptation for survival: phenotype and transcriptome response of CHO cells to elevated stress induced by agitation and sparging.
Sieck JB, Budach WE, Suemeghy Z, Leist C, Villiger TK, Morbidelli M, Soos M., J Biotechnol 189(), 2014
PMID: 25218361
CHO microRNA engineering is growing up: recent successes and future challenges.
Jadhav V, Hackl M, Druz A, Shridhar S, Chung CY, Heffner KM, Kreil DP, Betenbaugh M, Shiloach J, Barron N, Grillari J, Borth N., Biotechnol Adv 31(8), 2013
PMID: 23916872

8 References

Daten bereitgestellt von Europe PubMed Central.

The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line.
Xu X, Nagarajan H, Lewis NE, Pan S, Cai Z, Liu X, Chen W, Xie M, Wang W, Hammond S, Andersen MR, Neff N, Passarelli B, Koh W, Fan HC, Wang J, Gui Y, Lee KH, Betenbaugh MJ, Quake SR, Famili I, Palsson BO, Wang J., Nat. Biotechnol. 29(8), 2011
PMID: 21804562
Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome.
Lewis NE, Liu X, Li Y, Nagarajan H, Yerganian G, O'Brien E, Bordbar A, Roth AM, Rosenbloom J, Bian C, Xie M, Chen W, Li N, Baycin-Hizal D, Latif H, Forster J, Betenbaugh MJ, Famili I, Xu X, Wang J, Palsson BO., Nat. Biotechnol. 31(8), 2013
PMID: 23873082
Genetic characterization of CHO production host DG44 and derivative recombinant cell lines.
Derouazi M, Martinet D, Besuchet Schmutz N, Flaction R, Wicht M, Bertschinger M, Hacker DL, Beckmann JS, Wurm FM., Biochem. Biophys. Res. Commun. 340(4), 2005
PMID: 16403443
Construction of BAC-based physical map and analysis of chromosome rearrangement in Chinese hamster ovary cell lines.
Cao Y, Kimura S, Itoi T, Honda K, Ohtake H, Omasa T., Biotechnol. Bioeng. 109(6), 2011
PMID: 22012683
Limitations of next-generation genome sequence assembly.
Alkan C, Sajjadian S, Eichler EE., Nat. Methods 8(1), 2010
PMID: 21102452
Bacterial artificial chromosome library for genome-wide analysis of Chinese hamster ovary cells.
Omasa T, Cao Y, Park JY, Takagi Y, Kimura S, Yano H, Honda K, Asakawa S, Shimizu N, Ohtake H., Biotechnol. Bioeng. 104(5), 2009
PMID: 19575438
High-quality draft assemblies of mammalian genomes from massively parallel sequence data.
Gnerre S, Maccallum I, Przybylski D, Ribeiro FJ, Burton JN, Walker BJ, Sharpe T, Hall G, Shea TP, Sykes S, Berlin AM, Aird D, Costello M, Daza R, Williams L, Nicol R, Gnirke A, Nusbaum C, Lander ES, Jaffe DB., Proc. Natl. Acad. Sci. U.S.A. 108(4), 2010
PMID: 21187386


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