Reconstruction of the lipid metabolism for the microalga Monoraphidium neglectum from its genome sequence reveals characteristics suitable for biofuel production

Bogen C, Al-Dilaimi A, Albersmeier A, Wichmann J, Grundmann M, Rupp O, Lauersen KJ, Blifernez-Klassen O, Kalinowski J, Goesmann A, Mussgnug JH, et al. (2013)
BMC Genomics 14(1): 926.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
URN
urn:nbn:de:0070-pub-26456684
Autor*in
Bogen, ChristianUniBi; Al-Dilaimi, ArwaUniBi; Albersmeier, AndreasUniBi; Wichmann, JulianUniBi; Grundmann, MichaelUniBi; Rupp, OliverUniBi; Lauersen, Kyle J.UniBi ; Blifernez-Klassen, OlgaUniBi ; Kalinowski, JörnUniBi; Goesmann, AlexanderUniBi ; Mussgnug, Jan H.UniBi; Kruse, OlafUniBi
Einrichtung
Centrum für Biotechnologie > Arbeitsgruppe A. Goesmann
Technische Fakultät > Computational Genomics
Fakultät für Biologie > Algenbiotechnologie
Centrum für Biotechnologie > Arbeitsgruppe O. Kruse
Centrum für Biotechnologie > Graduate Center > Graduate Cluster Industrial Biotechnology
Abstract / Bemerkung
BACKGROUND: Microalgae are gaining importance as sustainable production hosts in the fields of biotechnology and bioenergy. A robust biomass accumulating strainof the genus Monoraphidium (SAG 48.87) was investigated in this work as apotential feedstock for biofuel production. The genome was sequenced, annotated, and key enzymes for triacylglycerol formation were elucidated. RESULTS: Monoraphidium neglectum was identified as an oleaginous species with favourable growth characteristics as well as a high potential for crude oil production, based on neutral lipid contents of approximately 21% (dry weight) under nitrogen starvation, composed of predominantly C18:1 and C16:0 fatty acids. Further characterization revealed growth in a relatively wide pH range and salt concentrations of up to 1.0% NaCl, in which the cells exhibited larger structures. This first full genome sequencing of a member of the Selenastraceae revealed a diploid, approximately 68 Mbp genome with a G + C content of 64.7%. The circular chloroplast genome was assembled to a 135,362 bp single contig, containing 67 protein-coding genes. The assembly of the mitochondrial genome resulted in two contigs with an approximate total size of 94 kb, the largest known mitochondrial genome within algae. 16,761 protein-coding genes were assigned to the nuclear genome. Comparison of gene sets with respect to functional categories revealed a higher gene number assigned to the category "carbohydrate metabolic process" and in "fatty acid biosynthetic process" in M. neglectum when compared to Chlamydomonas reinhardtii and Nannochloropsis gaditana, indicating a higher metabolic diversity for applications in carbohydrate conversions of biotechnological relevance. CONCLUSIONS: The genome of M. neglectum, as well as the metabolic reconstruction of crucial lipid pathways, provides new insights into the diversity of the lipid metabolism in microalgae. The results of this work provide a platform to encourage the development of this strain for biotechnological applications and production concepts.
Erscheinungsjahr
2013
Zeitschriftentitel
BMC Genomics
Band
14
Ausgabe
1
Art.-Nr.
926
ISSN
1471-2164
Page URI
https://pub.uni-bielefeld.de/record/2645668

Zitieren

Bogen C, Al-Dilaimi A, Albersmeier A, et al. Reconstruction of the lipid metabolism for the microalga Monoraphidium neglectum from its genome sequence reveals characteristics suitable for biofuel production. BMC Genomics. 2013;14(1): 926.
Bogen, C., Al-Dilaimi, A., Albersmeier, A., Wichmann, J., Grundmann, M., Rupp, O., Lauersen, K. J., et al. (2013). Reconstruction of the lipid metabolism for the microalga Monoraphidium neglectum from its genome sequence reveals characteristics suitable for biofuel production. BMC Genomics, 14(1), 926. doi:10.1186/1471-2164-14-926
Bogen, Christian, Al-Dilaimi, Arwa, Albersmeier, Andreas, Wichmann, Julian, Grundmann, Michael, Rupp, Oliver, Lauersen, Kyle J., et al. 2013. “Reconstruction of the lipid metabolism for the microalga Monoraphidium neglectum from its genome sequence reveals characteristics suitable for biofuel production”. BMC Genomics 14 (1): 926.
Bogen, C., Al-Dilaimi, A., Albersmeier, A., Wichmann, J., Grundmann, M., Rupp, O., Lauersen, K. J., Blifernez-Klassen, O., Kalinowski, J., Goesmann, A., et al. (2013). Reconstruction of the lipid metabolism for the microalga Monoraphidium neglectum from its genome sequence reveals characteristics suitable for biofuel production. BMC Genomics 14:926.
Bogen, C., et al., 2013. Reconstruction of the lipid metabolism for the microalga Monoraphidium neglectum from its genome sequence reveals characteristics suitable for biofuel production. BMC Genomics, 14(1): 926.
C. Bogen, et al., “Reconstruction of the lipid metabolism for the microalga Monoraphidium neglectum from its genome sequence reveals characteristics suitable for biofuel production”, BMC Genomics, vol. 14, 2013, : 926.
Bogen, C., Al-Dilaimi, A., Albersmeier, A., Wichmann, J., Grundmann, M., Rupp, O., Lauersen, K.J., Blifernez-Klassen, O., Kalinowski, J., Goesmann, A., Mussgnug, J.H., Kruse, O.: Reconstruction of the lipid metabolism for the microalga Monoraphidium neglectum from its genome sequence reveals characteristics suitable for biofuel production. BMC Genomics. 14, : 926 (2013).
Bogen, Christian, Al-Dilaimi, Arwa, Albersmeier, Andreas, Wichmann, Julian, Grundmann, Michael, Rupp, Oliver, Lauersen, Kyle J., Blifernez-Klassen, Olga, Kalinowski, Jörn, Goesmann, Alexander, Mussgnug, Jan H., and Kruse, Olaf. “Reconstruction of the lipid metabolism for the microalga Monoraphidium neglectum from its genome sequence reveals characteristics suitable for biofuel production”. BMC Genomics 14.1 (2013): 926.
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Review. Methodologies for transferring DNA into eukaryotic microalgae
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Characterization of a novel thioesterase (PtTE) from Phaeodactylum tricornutum.
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The response of diatom central carbon metabolism to nitrogen starvation is different from that of green algae and higher plants.
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A chloroplast pathway for the de novo biosynthesis of triacylglycerol in Chlamydomonas reinhardtii.
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A simple method for the isolation and purification of total lipides from animal tissues.
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Chlamydomonas starchless mutant defective in ADP-glucose pyrophosphorylase hyper-accumulates triacylglycerol.
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Viewing and Editing Assembled Sequences Using Consed
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RNAmmer: consistent and rapid annotation of ribosomal RNA genes.
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tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence
AUTHOR UNKNOWN, 1997
Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees
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MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.
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Claudel-Renard C, Chevalet C, Faraut T, Kahn D., Nucleic Acids Res. 31(22), 2003
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