IncP-1-beta plasmid pGNB1 isolated from a bacterial community from a wastewater treatment plant mediates decolorization of triphenylmethane dyes

Schlüter A, Krahn I, Kollin F, Bonemann G, Stiens M, Szczepanowski R, Schneiker-Bekel S, Pühler A (2007)
Appl Environ Microbiol 73(20): 6345-6350.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
Plasmid pGNB1 was isolated from bacteria residing in the activated sludge compartment of a wastewater treatment plant by using a transformation-based approach. This 60-kb plasmid confers resistance to the triphenylmethane dye crystal violet and enables its host bacterium to decolorize crystal violet. Partial sequencing of pGNB1 revealed that its backbone is very similar to that of previously sequenced IncP-1beta plasmids. The two accessory regions of the plasmid, one located downstream of the replication initiation gene trfA and the other located between the conjugative transfer modules Tra and Trb, were completely sequenced. Accessory region L1 contains a transposon related to Tn5501 and a gene encoding a Cupin 2 conserved barrel protein with an unknown function. The triphenylmethane reductase gene tmr and a truncated dihydrolipoamide dehydrogenase gene that is flanked by IS1071 and another putative insertion element were identified in accessory region L2. Subcloning of the pGNB1 tmr gene demonstrated that this gene is responsible for the observed crystal violet resistance phenotype and mediates decolorization of the triphenylmethane dyes crystal violet, malachite green, and basic fuchsin. Plasmid pGNB1 and the associated phenotype are transferable to the alpha-proteobacterium Sinorhizobium meliloti and the gamma-proteobacterium Escherichia coli. This is the first report of a promiscuous IncP-1beta plasmid isolated from the bacterial community from a wastewater treatment plant that harbors a triphenylmethane reductase gene. The pGNB1-encoded enzyme activity is discussed with respect to bioremediation of sewage polluted with triphenylmethane dyes.
Erscheinungsjahr
Zeitschriftentitel
Appl Environ Microbiol
Band
73
Ausgabe
20
Seite(n)
6345-6350
ISSN
PUB-ID

Zitieren

Schlüter A, Krahn I, Kollin F, et al. IncP-1-beta plasmid pGNB1 isolated from a bacterial community from a wastewater treatment plant mediates decolorization of triphenylmethane dyes. Appl Environ Microbiol. 2007;73(20):6345-6350.
Schlüter, A., Krahn, I., Kollin, F., Bonemann, G., Stiens, M., Szczepanowski, R., Schneiker-Bekel, S., et al. (2007). IncP-1-beta plasmid pGNB1 isolated from a bacterial community from a wastewater treatment plant mediates decolorization of triphenylmethane dyes. Appl Environ Microbiol, 73(20), 6345-6350. doi:10.1128/AEM.01177-07
Schlüter, A., Krahn, I., Kollin, F., Bonemann, G., Stiens, M., Szczepanowski, R., Schneiker-Bekel, S., and Pühler, A. (2007). IncP-1-beta plasmid pGNB1 isolated from a bacterial community from a wastewater treatment plant mediates decolorization of triphenylmethane dyes. Appl Environ Microbiol 73, 6345-6350.
Schlüter, A., et al., 2007. IncP-1-beta plasmid pGNB1 isolated from a bacterial community from a wastewater treatment plant mediates decolorization of triphenylmethane dyes. Appl Environ Microbiol, 73(20), p 6345-6350.
A. Schlüter, et al., “IncP-1-beta plasmid pGNB1 isolated from a bacterial community from a wastewater treatment plant mediates decolorization of triphenylmethane dyes”, Appl Environ Microbiol, vol. 73, 2007, pp. 6345-6350.
Schlüter, A., Krahn, I., Kollin, F., Bonemann, G., Stiens, M., Szczepanowski, R., Schneiker-Bekel, S., Pühler, A.: IncP-1-beta plasmid pGNB1 isolated from a bacterial community from a wastewater treatment plant mediates decolorization of triphenylmethane dyes. Appl Environ Microbiol. 73, 6345-6350 (2007).
Schlüter, Andreas, Krahn, Irene, Kollin, Florian, Bonemann, Gabriele, Stiens, Michael, Szczepanowski, Rafael, Schneiker-Bekel, Susanne, and Pühler, Alfred. “IncP-1-beta plasmid pGNB1 isolated from a bacterial community from a wastewater treatment plant mediates decolorization of triphenylmethane dyes”. Appl Environ Microbiol 73.20 (2007): 6345-6350.

15 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Persistence of antibiotic resistance and plasmid-associated genes in soil following application of sewage sludge and abundance on vegetables at harvest.
Rahube TO, Marti R, Scott A, Tien YC, Murray R, Sabourin L, Duenk P, Lapen DR, Topp E., Can J Microbiol 62(7), 2016
PMID: 27277701
Genetic characterization of plasmid-associated triphenylmethane reductase in Listeria monocytogenes.
Dutta V, Elhanafi D, Osborne J, Martinez MR, Kathariou S., Appl Environ Microbiol 80(17), 2014
PMID: 24951782
Diverse broad-host-range plasmids from freshwater carry few accessory genes.
Brown CJ, Sen D, Yano H, Bauer ML, Rogers LM, Van der Auwera GA, Top EM., Appl Environ Microbiol 79(24), 2013
PMID: 24096417
Role of IncP-1β plasmids pWDL7::rfp and pNB8c in chloroaniline catabolism as determined by genomic and functional analyses.
Król JE, Penrod JT, McCaslin H, Rogers LM, Yano H, Stancik AD, Dejonghe W, Brown CJ, Parales RE, Wuertz S, Top EM., Appl Environ Microbiol 78(3), 2012
PMID: 22101050
IncP-1β plasmids of Comamonas sp. and Delftia sp. strains isolated from a wastewater treatment plant mediate resistance to and decolorization of the triphenylmethane dye crystal violet.
Stolze Y, Eikmeyer F, Wibberg D, Brandis G, Karsten C, Krahn I, Schneiker-Bekel S, Viehöver P, Barsch A, Keck M, Top EM, Niehaus K, Schlüter A., Microbiology 158(pt 8), 2012
PMID: 22653947
Metagenomic profiling of microbial composition and antibiotic resistance determinants in Puget Sound.
Port JA, Wallace JC, Griffith WC, Faustman EM., PLoS One 7(10), 2012
PMID: 23144718
Biodegradation of leuco derivatives of triphenylmethane dyes by Sphingomonas sp. CM9.
Wu J, Li L, Du H, Jiang L, Zhang Q, Wei Z, Wang X, Xiao L, Yang L., Biodegradation 22(5), 2011
PMID: 21188476
Decolorizing activity of malachite green and its mechanisms involved in dye biodegradation by Achromobacter xylosoxidans MG1.
Wang J, Qiao M, Wei K, Ding J, Liu Z, Zhang KQ, Huang X., J Mol Microbiol Biotechnol 20(4), 2011
PMID: 21865764
Sequence and role in virulence of the three plasmid complement of the model tumor-inducing bacterium Pseudomonas savastanoi pv. savastanoi NCPPB 3335.
Bardaji L, Pérez-Martínez I, Rodríguez-Moreno L, Rodríguez-Palenzuela P, Sundin GW, Ramos C, Murillo J., PLoS One 6(10), 2011
PMID: 22022435
Phylogenetic characterization of a biogas plant microbial community integrating clone library 16S-rDNA sequences and metagenome sequence data obtained by 454-pyrosequencing.
Kröber M, Bekel T, Diaz NN, Goesmann A, Jaenicke S, Krause L, Miller D, Runte KJ, Viehöver P, Pühler A, Schlüter A., J Biotechnol 142(1), 2009
PMID: 19480946
Detection of 140 clinically relevant antibiotic-resistance genes in the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to selected antibiotics.
Szczepanowski R, Linke B, Krahn I, Gartemann KH, Gützkow T, Eichler W, Pühler A, Schlüter A., Microbiology 155(pt 7), 2009
PMID: 19389756
Genetic diversity and composition of a plasmid metagenome from a wastewater treatment plant.
Schlüter A, Krause L, Szczepanowski R, Goesmann A, Pühler A., J Biotechnol 136(1-2), 2008
PMID: 18603322
Insight into the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to antimicrobial drugs analysed by the 454-pyrosequencing technology.
Szczepanowski R, Bekel T, Goesmann A, Krause L, Krömeke H, Kaiser O, Eichler W, Pühler A, Schlüter A., J Biotechnol 136(1-2), 2008
PMID: 18586057

50 References

Daten bereitgestellt von Europe PubMed Central.

R factor transfer in Rhizobium leguminosarum.
Beringer JE., J. Gen. Microbiol. 84(1), 1974
PMID: 4612098
Genetic diversity among 3-chloroaniline- and aniline-degrading strains of the Comamonadaceae.
Boon N, Goris J, De Vos P, Verstraete W, Top EM., Appl. Environ. Microbiol. 67(3), 2001
PMID: 11229899

AUTHOR UNKNOWN, 1987

AUTHOR UNKNOWN, 2006
Genetic exchange between bacteria in the environment.
Davison J., Plasmid 42(2), 1999
PMID: 10489325
The evolution of IncP catabolic plasmids.
Dennis JJ., Curr. Opin. Biotechnol. 16(3), 2005
PMID: 15961030
Structures of homologous composite transposons carrying cbaABC genes from Europe and North America.
Di Gioia D, Peel M, Fava F, Wyndham RC., Appl. Environ. Microbiol. 64(5), 1998
PMID: 9572977
Mobile genetic elements: the agents of open source evolution.
Frost LS, Leplae R, Summers AO, Toussaint A., Nat. Rev. Microbiol. 3(9), 2005
PMID: 16138100
Consed: a graphical tool for sequence finishing.
Gordon D, Abajian C, Green P., Genome Res. 8(3), 1998
PMID: 9521923
Automated finishing with autofinish.
Gordon D, Desmarais C, Green P., Genome Res. 11(4), 2001
PMID: 11282977
Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants.
Grant SG, Jessee J, Bloom FR, Hanahan D., Proc. Natl. Acad. Sci. U.S.A. 87(12), 1990
PMID: 2162051
The complete sequences of plasmids pB2 and pB3 provide evidence for a recent ancestor of the IncP-1beta group without any accessory genes.
Heuer H, Szczepanowski R, Schneiker S, Puhler A, Top EM, Schluter A., Microbiology (Reading, Engl.) 150(Pt 11), 2004
PMID: 15528648

AUTHOR UNKNOWN, 2004
Decolorization of malachite green and crystal violet by waterborne pathogenic mycobacteria.
Jones JJ, Falkinham JO 3rd., Antimicrob. Agents Chemother. 47(7), 2003
PMID: 12821489
Complete nucleotide sequence and organization of the atrazine catabolic plasmid pADP-1 from Pseudomonas sp. strain ADP.
Martinez B, Tomkins J, Wackett LP, Wing R, Sadowsky MJ., J. Bacteriol. 183(19), 2001
PMID: 11544232
Functional analysis of the cysteine motifs in the ferredoxin-like protein FdxN of Rhizobium meliloti involved in symbiotic nitrogen fixation.
Masepohl B, Kutsche M, Riedel KU, Schmehl M, Klipp W, Puhler A., Mol. Gen. Genet. 233(1-2), 1992
PMID: 1603075
N-acetyltaurine dissimilated via taurine by Delftia acidovorans NAT.
Mayer J, Denger K, Smits TH, Hollemeyer K, Groth U, Cook AM., Arch. Microbiol. 186(1), 2006
PMID: 16802176
GenDB--an open source genome annotation system for prokaryote genomes.
Meyer F, Goesmann A, McHardy AC, Bartels D, Bekel T, Clausen J, Kalinowski J, Linke B, Rupp O, Giegerich R, Puhler A., Nucleic Acids Res. 31(8), 2003
PMID: 12682369

AUTHOR UNKNOWN, 1972
NorM, a putative multidrug efflux protein, of Vibrio parahaemolyticus and its homolog in Escherichia coli.
Morita Y, Kodama K, Shiota S, Mine T, Kataoka A, Mizushima T, Tsuchiya T., Antimicrob. Agents Chemother. 42(7), 1998
PMID: 9661020
Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species.
Nelson KE, Fouts DE, Mongodin EF, Ravel J, DeBoy RT, Kolonay JF, Rasko DA, Angiuoli SV, Gill SR, Paulsen IT, Peterson J, White O, Nelson WC, Nierman W, Beanan MJ, Brinkac LM, Daugherty SC, Dodson RJ, Durkin AS, Madupu R, Haft DH, Selengut J, Van Aken S, Khouri H, Fedorova N, Forberger H, Tran B, Kathariou S, Wonderling LD, Uhlich GA, Bayles DO, Luchansky JB, Fraser CM., Nucleic Acids Res. 32(8), 2004
PMID: 15115801
Divergence of mobile genetic elements involved in the distribution of xenobiotic-catabolic capacity.
Nojiri H, Shintani M, Omori T., Appl. Microbiol. Biotechnol. 64(2), 2003
PMID: 14689248
Azoreductase activity of anaerobic bacteria isolated from human intestinal microflora.
Rafii F, Franklin W, Cerniglia CE., Appl. Environ. Microbiol. 56(7), 1990
PMID: 2202258
Decolorization of triphenylmethane, azo, and anthraquinone dyes by a newly isolated Aeromonas hydrophila strain.
Ren S, Guo J, Zeng G, Sun G., Appl. Microbiol. Biotechnol. 72(6), 2006
PMID: 16622679
Arthrobacter aurescens TC1 atrazine catabolism genes trzN, atzB, and atzC are linked on a 160-kilobase region and are functional in Escherichia coli.
Sajjaphan K, Shapir N, Wackett LP, Palmer M, Blackmon B, Tomkins J, Sadowsky MJ., Appl. Environ. Microbiol. 70(7), 2004
PMID: 15240330

AUTHOR UNKNOWN, 1989
Novel macrolide resistance module carried by the IncP-1beta resistance plasmid pRSB111, isolated from a wastewater treatment plant.
Szczepanowski R, Krahn I, Bohn N, Puhler A, Schluter A., Antimicrob. Agents Chemother. 51(2), 2006
PMID: 17101677
Bacterial catabolic transposons.
Tan HM., Appl. Microbiol. Biotechnol. 51(1), 1999
PMID: 10077818
Mechanisms of, and barriers to, horizontal gene transfer between bacteria.
Thomas CM, Nielsen KM., Nat. Rev. Microbiol. 3(9), 2005
PMID: 16138099
Complete sequence of the IncPbeta plasmid R751: implications for evolution and organisation of the IncP backbone.
Thorsted PB, Macartney DP, Akhtar P, Haines AS, Ali N, Davidson P, Stafford T, Pocklington MJ, Pansegrau W, Wilkins BM, Lanka E, Thomas CM., J. Mol. Biol. 282(5), 1998
PMID: 9753548
The role of mobile genetic elements in bacterial adaptation to xenobiotic organic compounds.
Top EM, Springael D., Curr. Opin. Biotechnol. 14(3), 2003
PMID: 12849778
Genetic organization of the catabolic plasmid pJP4 from Ralstonia eutropha JMP134 (pJP4) reveals mechanisms of adaptation to chloroaromatic pollutants and evolution of specialized chloroaromatic degradation pathways.
Trefault N, De la Iglesia R, Molina AM, Manzano M, Ledger T, Perez-Pantoja D, Sanchez MA, Stuardo M, Gonzalez B., Environ. Microbiol. 6(7), 2004
PMID: 15186344

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 17675426
PubMed | Europe PMC

Suchen in

Google Scholar