Effect of Growth Media on the Diversity of Neocallimastigomycetes from Non-Rumen Habitats

Joshi A, Young D, Huang L, Mosberger L, Munk B, Vinzelj J, Flad V, Sczyrba A, Griffith GW, Podmirseg SM, Warthmann R, et al. (2022)
Microorganisms 10(10): 1972.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Joshi, Akshay; Young, Diana; Huang, LirenUniBi; Mosberger, Lona; Munk, Bernhard; Vinzelj, Julia; Flad, Veronika; Sczyrba, AlexanderUniBi ; Griffith, Gareth W.; Podmirseg, Sabine Marie; Warthmann, Rolf; Lebuhn, Michael
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Abstract / Bemerkung
Anaerobic fungi (AF), belonging to the phylum Neocallimastigomycota, are a pivotal component of the digestive tract microbiome of various herbivorous animals. In the last decade, the diversity of AF has rapidly expanded due to the exploration of numerous (novel) habitats. Studies aiming at understanding the role of AF require robust and reliable isolation and cultivation techniques, many of which remained unchanged for decades. Using amplicon sequencing, we compared three different media: medium with rumen fluid (RF), depleted rumen fluid (DRF), and no rumen fluid (NRF) to enrich the AF from the feces of yak, as a rumen control; and Przewalski’s horse, llama, guanaco, and elephant, as a non-rumen habitats. The results revealed the selective enrichment of Piromyces and Neocallimastix from the feces of elephant and llama, respectively, in the RF medium. Similarly, the enrichment culture in DRF medium explicitly manifested Piromyces-related sequences from elephant feces. Five new clades (MM1-5) were defined from llama, guanaco, yak, and elephant feces that could as well be enriched from llama and elephant samples using non-conventional DRF and NRF media. This study presents evidence for the selective enrichment of certain genera in medium with RF and DRF from rumen as well as from non-rumen samples. NRF medium is suggested for the isolation of AF from non-rumen environments.
Erscheinungsjahr
2022
Zeitschriftentitel
Microorganisms
Band
10
Ausgabe
10
Art.-Nr.
1972
eISSN
2076-2607
Page URI
https://pub.uni-bielefeld.de/record/2966286

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Joshi A, Young D, Huang L, et al. Effect of Growth Media on the Diversity of Neocallimastigomycetes from Non-Rumen Habitats. Microorganisms. 2022;10(10): 1972.
Joshi, A., Young, D., Huang, L., Mosberger, L., Munk, B., Vinzelj, J., Flad, V., et al. (2022). Effect of Growth Media on the Diversity of Neocallimastigomycetes from Non-Rumen Habitats. Microorganisms, 10(10), 1972. https://doi.org/10.3390/microorganisms10101972
Joshi, Akshay, Young, Diana, Huang, Liren, Mosberger, Lona, Munk, Bernhard, Vinzelj, Julia, Flad, Veronika, et al. 2022. “Effect of Growth Media on the Diversity of Neocallimastigomycetes from Non-Rumen Habitats”. Microorganisms 10 (10): 1972.
Joshi, A., Young, D., Huang, L., Mosberger, L., Munk, B., Vinzelj, J., Flad, V., Sczyrba, A., Griffith, G. W., Podmirseg, S. M., et al. (2022). Effect of Growth Media on the Diversity of Neocallimastigomycetes from Non-Rumen Habitats. Microorganisms 10:1972.
Joshi, A., et al., 2022. Effect of Growth Media on the Diversity of Neocallimastigomycetes from Non-Rumen Habitats. Microorganisms, 10(10): 1972.
A. Joshi, et al., “Effect of Growth Media on the Diversity of Neocallimastigomycetes from Non-Rumen Habitats”, Microorganisms, vol. 10, 2022, : 1972.
Joshi, A., Young, D., Huang, L., Mosberger, L., Munk, B., Vinzelj, J., Flad, V., Sczyrba, A., Griffith, G.W., Podmirseg, S.M., Warthmann, R., Lebuhn, M., Insam, H.: Effect of Growth Media on the Diversity of Neocallimastigomycetes from Non-Rumen Habitats. Microorganisms. 10, : 1972 (2022).
Joshi, Akshay, Young, Diana, Huang, Liren, Mosberger, Lona, Munk, Bernhard, Vinzelj, Julia, Flad, Veronika, Sczyrba, Alexander, Griffith, Gareth W., Podmirseg, Sabine Marie, Warthmann, Rolf, Lebuhn, Michael, and Insam, Heribert. “Effect of Growth Media on the Diversity of Neocallimastigomycetes from Non-Rumen Habitats”. Microorganisms 10.10 (2022): 1972.
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