Importance of Defluviitalea raffinosedens for Hydrolytic Biomass Degradation in Co-Culture with Hungateiclostridium thermocellum

Rettenmaier R, Schneider M, Munk B, Lebuhn M, Jünemann S, Sczyrba A, Maus I, Zverlov V, Liebl W (2020)
Microorganisms 8(6): 915.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Rettenmaier, Regina; Schneider, Martina; Munk, Bernhard; Lebuhn, Michael; Jünemann, SebastianUniBi; Sczyrba, AlexanderUniBi ; Maus, IrenaUniBi; Zverlov, Vladimir; Liebl, Wolfgang
Abstract / Bemerkung
Bacterial hydrolysis of polysaccharides is an important step for the production of sustainable energy, for example during the conversion of plant biomass to methane-rich biogas. Previously, Hungateiclostridium thermocellum was identified as cellulolytic key player in thermophilic biogas microbiomes with a great frequency as an accompanying organism. The aim of this study was to physiologically characterize a recently isolated co-culture of H. thermocellum and the saccharolytic bacterium Defluviitalea raffinosedens from a laboratory-scale biogas fermenter. The characterization focused on cellulose breakdown by applying the measurement of cellulose hydrolysis, production of metabolites, and the activity of secreted enzymes. Substrate degradation and the production of volatile metabolites was considerably enhanced when both organisms acted synergistically. The metabolic properties of H. thermocellum have been studied well in the past. To predict the role of D. raffinosedens in this bacterial duet, the genome of D. raffinosedens was sequenced for the first time. Concomitantly, to deduce the prevalence of D. raffinosedens in anaerobic digestion, taxonomic composition and transcriptional activity of different biogas microbiomes were analyzed in detail. Defluviitalea was abundant and metabolically active in reactor operating at highly efficient process conditions, supporting the importance of this organism for the hydrolysis of the raw substrate.
Stichworte
biogas; metagenomics; whole-genome sequencing; cellulose degradation; carbohydrate active enzymes; metabolic interaction
Erscheinungsjahr
2020
Zeitschriftentitel
Microorganisms
Band
8
Ausgabe
6
Art.-Nr.
915
ISSN
2076-2607
Page URI
https://pub.uni-bielefeld.de/record/2944226

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Rettenmaier R, Schneider M, Munk B, et al. Importance of Defluviitalea raffinosedens for Hydrolytic Biomass Degradation in Co-Culture with Hungateiclostridium thermocellum. Microorganisms. 2020;8(6): 915.
Rettenmaier, R., Schneider, M., Munk, B., Lebuhn, M., Jünemann, S., Sczyrba, A., Maus, I., et al. (2020). Importance of Defluviitalea raffinosedens for Hydrolytic Biomass Degradation in Co-Culture with Hungateiclostridium thermocellum. Microorganisms, 8(6), 915. doi:10.3390/microorganisms8060915
Rettenmaier, R., Schneider, M., Munk, B., Lebuhn, M., Jünemann, S., Sczyrba, A., Maus, I., Zverlov, V., and Liebl, W. (2020). Importance of Defluviitalea raffinosedens for Hydrolytic Biomass Degradation in Co-Culture with Hungateiclostridium thermocellum. Microorganisms 8:915.
Rettenmaier, R., et al., 2020. Importance of Defluviitalea raffinosedens for Hydrolytic Biomass Degradation in Co-Culture with Hungateiclostridium thermocellum. Microorganisms, 8(6): 915.
R. Rettenmaier, et al., “Importance of Defluviitalea raffinosedens for Hydrolytic Biomass Degradation in Co-Culture with Hungateiclostridium thermocellum”, Microorganisms, vol. 8, 2020, : 915.
Rettenmaier, R., Schneider, M., Munk, B., Lebuhn, M., Jünemann, S., Sczyrba, A., Maus, I., Zverlov, V., Liebl, W.: Importance of Defluviitalea raffinosedens for Hydrolytic Biomass Degradation in Co-Culture with Hungateiclostridium thermocellum. Microorganisms. 8, : 915 (2020).
Rettenmaier, Regina, Schneider, Martina, Munk, Bernhard, Lebuhn, Michael, Jünemann, Sebastian, Sczyrba, Alexander, Maus, Irena, Zverlov, Vladimir, and Liebl, Wolfgang. “Importance of Defluviitalea raffinosedens for Hydrolytic Biomass Degradation in Co-Culture with Hungateiclostridium thermocellum”. Microorganisms 8.6 (2020): 915.
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2020-06-23T07:12:05Z
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