Comparative genomics reveals phylogenetic distribution patterns of secondary metabolites in Amycolatopsis species

Adamek M, Alanjary M, Sales-Ortells H, Goodfellow M, Bull AT, Winkler A, Wibberg D, Kalinowski J, Ziemert N (2018)
BMC GENOMICS 19: 15.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Autor/in
; ; ; ; ; ; ; ;
Abstract / Bemerkung
Background: Genome mining tools have enabled us to predict biosynthetic gene clusters that might encode compounds with valuable functions for industrial and medical applications. With the continuously increasing number of genomes sequenced, we are confronted with an overwhelming number of predicted clusters. In order to guide the effective prioritization of biosynthetic gene clusters towards finding the most promising compounds, knowledge about diversity, phylogenetic relationships and distribution patterns of biosynthetic gene clusters is necessary. Results: Here, we provide a comprehensive analysis of the mode ! actinobacterial genus Amycoiatopsis and its potential for the production of secondary metabolites. A phylogenetic characterization, together with a pan-genome analysis showed that within this highly diverse genus, four major lineages could be distinguished which differed in their potential to produce secondary metabolites. Furthermore, we were able to distinguish gene cluster families whose distribution correlated with phylogeny, indicating that vertical gene transfer plays a major role in the evolution of secondary metabolite gene clusters. Still, the vast majority of the diverse biosynthetic gene clusters were derived from clusters unique to the genus, and also unique in comparison to a database of known compounds. Our study on the locations of biosynthetic gene clusters in the genomes of Amycoiatopsis' strains showed that clusters acquired by horizontal gene transfer tend to be incorporated into non conserved regions of the genome thereby allowing us to distinguish core and hypervariable regions in Amycoiatopsis genomes. Conclusions: Using a comparative genomics approach, it was possible to determine the potential of the genus Amycoiatopsis to produce a huge diversity of secondary metabolites. Furthermore, the analysis demonstrates that horizontal and vertical gene transfer play an important role in the acquisition and maintenance of valuable secondary metabolites. Our results cast light on the interconnections between secondary metabolite gene clusters and provide a way to prioritize biosynthetic pathways in the search and discovery of novel compounds.
Stichworte
Amycolatopsis; Genome mining; Comparative genomics; Biosynthetic gene; cluster; Gene cluster family; Secondary metabolite diversity; Phylogeny; Natural products; Evolution
Erscheinungsjahr
2018
Zeitschriftentitel
BMC GENOMICS
Band
19
Art.-Nr.
15
ISSN
1471-2164
Page URI
https://pub.uni-bielefeld.de/record/2920680

Zitieren

Adamek M, Alanjary M, Sales-Ortells H, et al. Comparative genomics reveals phylogenetic distribution patterns of secondary metabolites in Amycolatopsis species. BMC GENOMICS. 2018;19: 15.
Adamek, M., Alanjary, M., Sales-Ortells, H., Goodfellow, M., Bull, A. T., Winkler, A., Wibberg, D., et al. (2018). Comparative genomics reveals phylogenetic distribution patterns of secondary metabolites in Amycolatopsis species. BMC GENOMICS, 19, 15. doi:10.1186/S12864-018-4809-4
Adamek, M., Alanjary, M., Sales-Ortells, H., Goodfellow, M., Bull, A. T., Winkler, A., Wibberg, D., Kalinowski, J., and Ziemert, N. (2018). Comparative genomics reveals phylogenetic distribution patterns of secondary metabolites in Amycolatopsis species. BMC GENOMICS 19:15.
Adamek, M., et al., 2018. Comparative genomics reveals phylogenetic distribution patterns of secondary metabolites in Amycolatopsis species. BMC GENOMICS, 19: 15.
M. Adamek, et al., “Comparative genomics reveals phylogenetic distribution patterns of secondary metabolites in Amycolatopsis species”, BMC GENOMICS, vol. 19, 2018, : 15.
Adamek, M., Alanjary, M., Sales-Ortells, H., Goodfellow, M., Bull, A.T., Winkler, A., Wibberg, D., Kalinowski, J., Ziemert, N.: Comparative genomics reveals phylogenetic distribution patterns of secondary metabolites in Amycolatopsis species. BMC GENOMICS. 19, : 15 (2018).
Adamek, Martina, Alanjary, Mohammad, Sales-Ortells, Helena, Goodfellow, Michael, Bull, Alan T., Winkler, Anika, Wibberg, Daniel, Kalinowski, Jörn, and Ziemert, Nadine. “Comparative genomics reveals phylogenetic distribution patterns of secondary metabolites in Amycolatopsis species”. BMC GENOMICS 19 (2018): 15.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 29859036
PubMed | Europe PMC

Suchen in

Google Scholar