Genome content, metabolic pathways and biotechnological potential of the psychrophilic Arctic bacterium Psychrobacter sp DAB_AL43B, a source and a host of novel Psychrobacter-specific vectors

Lasek R, Dziewit L, Ciok A, Decewicz P, Romaniuk K, Jedrys Z, Wibberg D, Schlüter A, Pühler A, Bartosik D (2017)
Journal of Biotechnology 263: 64-74.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Lasek, Robert; Dziewit, Lukasz; Ciok, Anna; Decewicz, Przemyslaw; Romaniuk, Krzysztof; Jedrys, Zuzanna; Wibberg, DanielUniBi; Schlüter, AndreasUniBi ; Pühler, AlfredUniBi ; Bartosik, Dariusz
Abstract / Bemerkung
Psychrobacter sp. DAB_AL43B, isolated from ornithogenic soil collected on the Arctic island of Spitsbergen, is a newly sequenced psychrophilic strain susceptible to conjugation and electrotransformation. Its genome consists of a circular chromosome (3.3 Mb) and four plasmids (4.4-6.4 kb). In silico genome mining and microarray-based phenotypic analysis were performed to describe the metabolic potential of this strain and identify possible biotechnological applications. Metabolic reconstruction indicated that DAB_AL43B prefers low-molecular-weight carboxylates and amino acids as carbon and energy sources. Genetic determinants of heavy-metal resistance, anthracene degradation and possible aerobic denitrification were also identified. Comparative analyses revealed a relatively close relationship between DAB_AL43B and other sequenced Psychrobacter species. In addition, the plasmids of this strain were used as the basis for the construction of Escherichia coli-Psychrobacter spp. shuttle vectors. Taken together, the results of this work suggest that DAB_AL43B is a promising candidate as a new model strain for studies on Psychrobacter spp.
Stichworte
Psychrobacter sp; Psychrophile; Complete genome; Metabolic; reconstruction; Phenotypic microarrays; Comparative genomics
Erscheinungsjahr
2017
Zeitschriftentitel
Journal of Biotechnology
Band
263
Seite(n)
64-74
ISSN
0168-1656
eISSN
1873-4863
Page URI
https://pub.uni-bielefeld.de/record/2916406

Zitieren

Lasek R, Dziewit L, Ciok A, et al. Genome content, metabolic pathways and biotechnological potential of the psychrophilic Arctic bacterium Psychrobacter sp DAB_AL43B, a source and a host of novel Psychrobacter-specific vectors. Journal of Biotechnology. 2017;263:64-74.
Lasek, R., Dziewit, L., Ciok, A., Decewicz, P., Romaniuk, K., Jedrys, Z., Wibberg, D., et al. (2017). Genome content, metabolic pathways and biotechnological potential of the psychrophilic Arctic bacterium Psychrobacter sp DAB_AL43B, a source and a host of novel Psychrobacter-specific vectors. Journal of Biotechnology, 263, 64-74. doi:10.1016/j.jbiotec.2017.09.011
Lasek, Robert, Dziewit, Lukasz, Ciok, Anna, Decewicz, Przemyslaw, Romaniuk, Krzysztof, Jedrys, Zuzanna, Wibberg, Daniel, Schlüter, Andreas, Pühler, Alfred, and Bartosik, Dariusz. 2017. “Genome content, metabolic pathways and biotechnological potential of the psychrophilic Arctic bacterium Psychrobacter sp DAB_AL43B, a source and a host of novel Psychrobacter-specific vectors”. Journal of Biotechnology 263: 64-74.
Lasek, R., Dziewit, L., Ciok, A., Decewicz, P., Romaniuk, K., Jedrys, Z., Wibberg, D., Schlüter, A., Pühler, A., and Bartosik, D. (2017). Genome content, metabolic pathways and biotechnological potential of the psychrophilic Arctic bacterium Psychrobacter sp DAB_AL43B, a source and a host of novel Psychrobacter-specific vectors. Journal of Biotechnology 263, 64-74.
Lasek, R., et al., 2017. Genome content, metabolic pathways and biotechnological potential of the psychrophilic Arctic bacterium Psychrobacter sp DAB_AL43B, a source and a host of novel Psychrobacter-specific vectors. Journal of Biotechnology, 263, p 64-74.
R. Lasek, et al., “Genome content, metabolic pathways and biotechnological potential of the psychrophilic Arctic bacterium Psychrobacter sp DAB_AL43B, a source and a host of novel Psychrobacter-specific vectors”, Journal of Biotechnology, vol. 263, 2017, pp. 64-74.
Lasek, R., Dziewit, L., Ciok, A., Decewicz, P., Romaniuk, K., Jedrys, Z., Wibberg, D., Schlüter, A., Pühler, A., Bartosik, D.: Genome content, metabolic pathways and biotechnological potential of the psychrophilic Arctic bacterium Psychrobacter sp DAB_AL43B, a source and a host of novel Psychrobacter-specific vectors. Journal of Biotechnology. 263, 64-74 (2017).
Lasek, Robert, Dziewit, Lukasz, Ciok, Anna, Decewicz, Przemyslaw, Romaniuk, Krzysztof, Jedrys, Zuzanna, Wibberg, Daniel, Schlüter, Andreas, Pühler, Alfred, and Bartosik, Dariusz. “Genome content, metabolic pathways and biotechnological potential of the psychrophilic Arctic bacterium Psychrobacter sp DAB_AL43B, a source and a host of novel Psychrobacter-specific vectors”. Journal of Biotechnology 263 (2017): 64-74.

3 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Genome Structure of the Opportunistic Pathogen Paracoccus yeei (Alphaproteobacteria) and Identification of Putative Virulence Factors.
Lasek R, Szuplewska M, Mitura M, Decewicz P, Chmielowska C, Pawłot A, Sentkowska D, Czarnecki J, Bartosik D., Front Microbiol 9(), 2018
PMID: 30410477

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