The DNA methylation landscape of Chinese hamster ovary (CHO) DP-12 cells
Wippermann A, Rupp O, Brinkrolf K, Hoffrogge R, Noll T (2015)
Journal of Biotechnology 199: 38-46.
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Abstract / Bemerkung
Chinese hamster ovary (CHO) cells represent the most commonly used production cell line for therapeutic proteins. By recent genome and transcriptome sequencing a basis was created for future investigations of genotype-phenotype relationships and for improvement of CHO cell productivity and product quality. In this context information is missing about DNA cytosine methylation as a crucial epigenetic modification and an important element in mammalian genome regulation and development. Here, we present the first DNA methylation map of a CHO cell line in single-base resolution that was generated by whole genome bisulfite sequencing combined with gene expression analysis by CHO microarrays. We show CHO DP-12 cells to exhibit global hypomethylation compared to a majority of mammalian methylomes and hypermethylation of CpG-dense regions at gene promoters called CpG islands. We also observed partially methylated domains that cover 62% of the CHO DP-12 cell genome and contain functional clusters of genes. Gene expression analysis showed these clusters to be either highly or weakly expressed with regard to CHO-specific characteristics and hence proves DNA methylation in CHO cells to be an important link between genomics and transcriptomics.
Stichworte
CHO;
Epigenetics;
DNA methylation;
Whole genome bisulfite sequencing;
Gene expression
Erscheinungsjahr
2015
Zeitschriftentitel
Journal of Biotechnology
Band
199
Seite(n)
38-46
ISSN
0168-1656
eISSN
1873-4863
Page URI
https://pub.uni-bielefeld.de/record/2718773
Zitieren
Wippermann A, Rupp O, Brinkrolf K, Hoffrogge R, Noll T. The DNA methylation landscape of Chinese hamster ovary (CHO) DP-12 cells. Journal of Biotechnology. 2015;199:38-46.
Wippermann, A., Rupp, O., Brinkrolf, K., Hoffrogge, R., & Noll, T. (2015). The DNA methylation landscape of Chinese hamster ovary (CHO) DP-12 cells. Journal of Biotechnology, 199, 38-46. doi:10.1016/j.jbiotec.2015.01.014
Wippermann, Anna, Rupp, Oliver, Brinkrolf, Karina, Hoffrogge, Raimund, and Noll, Thomas. 2015. “The DNA methylation landscape of Chinese hamster ovary (CHO) DP-12 cells”. Journal of Biotechnology 199: 38-46.
Wippermann, A., Rupp, O., Brinkrolf, K., Hoffrogge, R., and Noll, T. (2015). The DNA methylation landscape of Chinese hamster ovary (CHO) DP-12 cells. Journal of Biotechnology 199, 38-46.
Wippermann, A., et al., 2015. The DNA methylation landscape of Chinese hamster ovary (CHO) DP-12 cells. Journal of Biotechnology, 199, p 38-46.
A. Wippermann, et al., “The DNA methylation landscape of Chinese hamster ovary (CHO) DP-12 cells”, Journal of Biotechnology, vol. 199, 2015, pp. 38-46.
Wippermann, A., Rupp, O., Brinkrolf, K., Hoffrogge, R., Noll, T.: The DNA methylation landscape of Chinese hamster ovary (CHO) DP-12 cells. Journal of Biotechnology. 199, 38-46 (2015).
Wippermann, Anna, Rupp, Oliver, Brinkrolf, Karina, Hoffrogge, Raimund, and Noll, Thomas. “The DNA methylation landscape of Chinese hamster ovary (CHO) DP-12 cells”. Journal of Biotechnology 199 (2015): 38-46.
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