Establishment of blood glycosidase activities and their excursions in sepsis

Haslund-Gourley BS, Aziz PV, Heithoff DM, Restagno D, Fried JC, Ilse M-B, Bäumges H, Mahan MJ, Lübke T, Marth JD (2022)
PNAS Nexus 1(3): pgac113.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Haslund-Gourley, Benjamin S; Aziz, Peter V; Heithoff, Douglas M; Restagno, Damien; Fried, Jeffrey C; Ilse, Mai-BrittUniBi; Bäumges, HannahUniBi; Mahan, Michael J; Lübke, TorbenUniBi ; Marth, Jamey D
Abstract / Bemerkung
Glycosidases are hydrolytic enzymes studied principally in the context of intracellular catabolism within the lysosome. Therefore, glycosidase activities are classically measured in experimentally acidified assay conditions reflecting their low pH optima. However, glycosidases are also present in the bloodstream where they may retain sufficient activity to participate in the regulation of glycoprotein half-lives, proteostasis, and disease pathogenesis. We have, herein, established at physiological pH 7.4 in blood plasma and sera the normal ranges of four major glycosidase activities essential for blood glycoprotein remodeling in healthy mice and humans. These activities included β-galactosidase, β-N-acetylglucosaminidase, α-mannosidase, and α-fucosidase. We have identified their origins to include the mammalian genes Glb1, HexB, Man2a1, and Fuca1. In experimental sepsis, excursions of glycosidase activities occurred with differences in host responses to discrete bacterial pathogens. Among similar excursions in human sepsis, the elevation of β-galactosidase activity was a prognostic indicator of increased likelihood of patient death.
Erscheinungsjahr
2022
Zeitschriftentitel
PNAS Nexus
Band
1
Ausgabe
3
Art.-Nr.
pgac113
eISSN
2752-6542
Page URI
https://pub.uni-bielefeld.de/record/2965167

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Haslund-Gourley BS, Aziz PV, Heithoff DM, et al. Establishment of blood glycosidase activities and their excursions in sepsis. PNAS Nexus. 2022;1(3): pgac113.
Haslund-Gourley, B. S., Aziz, P. V., Heithoff, D. M., Restagno, D., Fried, J. C., Ilse, M. - B., Bäumges, H., et al. (2022). Establishment of blood glycosidase activities and their excursions in sepsis. PNAS Nexus, 1(3), pgac113. https://doi.org/10.1093/pnasnexus/pgac113
Haslund-Gourley, Benjamin S, Aziz, Peter V, Heithoff, Douglas M, Restagno, Damien, Fried, Jeffrey C, Ilse, Mai-Britt, Bäumges, Hannah, Mahan, Michael J, Lübke, Torben, and Marth, Jamey D. 2022. “Establishment of blood glycosidase activities and their excursions in sepsis”. PNAS Nexus 1 (3): pgac113.
Haslund-Gourley, B. S., Aziz, P. V., Heithoff, D. M., Restagno, D., Fried, J. C., Ilse, M. - B., Bäumges, H., Mahan, M. J., Lübke, T., and Marth, J. D. (2022). Establishment of blood glycosidase activities and their excursions in sepsis. PNAS Nexus 1:pgac113.
Haslund-Gourley, B.S., et al., 2022. Establishment of blood glycosidase activities and their excursions in sepsis. PNAS Nexus, 1(3): pgac113.
B.S. Haslund-Gourley, et al., “Establishment of blood glycosidase activities and their excursions in sepsis”, PNAS Nexus, vol. 1, 2022, : pgac113.
Haslund-Gourley, B.S., Aziz, P.V., Heithoff, D.M., Restagno, D., Fried, J.C., Ilse, M.-B., Bäumges, H., Mahan, M.J., Lübke, T., Marth, J.D.: Establishment of blood glycosidase activities and their excursions in sepsis. PNAS Nexus. 1, : pgac113 (2022).
Haslund-Gourley, Benjamin S, Aziz, Peter V, Heithoff, Douglas M, Restagno, Damien, Fried, Jeffrey C, Ilse, Mai-Britt, Bäumges, Hannah, Mahan, Michael J, Lübke, Torben, and Marth, Jamey D. “Establishment of blood glycosidase activities and their excursions in sepsis”. PNAS Nexus 1.3 (2022): pgac113.

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