Reprogramming of Yersinia from Virulent to Persistent Mode Revealed by Complex In Vivo RNA-seq Analysis

Avican K, Fahlgren A, Huss M, Heroven AK, Beckstette M, Dersch P, Fällman M (2015)
PLOS Pathogens 11(1): e1004600.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
Es wurden keine Dateien hochgeladen. Nur Publikationsnachweis!
Autor*in
Avican, Kemal; Fahlgren, Anna; Huss, Mikael; Heroven, Ann Kathrin; Beckstette, MichaelUniBi; Dersch, Petra; Fällman, Maria
Abstract / Bemerkung
We recently found that Yersinia pseudotuberculosis can be used as a model of persistent bacterial infections. We performed in vivo RNA-seq of bacteria in small cecal tissue biopsies at early and persistent stages of infection to determine strategies associated with persistence. Comprehensive analysis of mixed RNA populations from infected tissues revealed that Y. pseudotuberculosis undergoes transcriptional reprogramming with drastic down-regulation of T3SS virulence genes during persistence when the pathogen resides within the cecum. At the persistent stage, the expression pattern in many respects resembles the pattern seen in vitro at 26oC, with for example, up-regulation of flagellar genes and invA. These findings are expected to have impact on future rationales to identify suitable bacterial targets for new antibiotics. Other genes that are up-regulated during persistence are genes involved in anaerobiosis, chemotaxis, and protection against oxidative and acidic stress, which indicates the influence of different environmental cues. We found that the Crp/CsrA/RovA regulatory cascades influence the pattern of bacterial gene expression during persistence. Furthermore, arcA, fnr, frdA, and wrbA play critical roles in persistence. Our findings suggest a model for the life cycle of this enteropathogen with reprogramming from a virulent to an adapted phenotype capable of persisting and spreading by fecal shedding.
Erscheinungsjahr
2015
Zeitschriftentitel
PLOS Pathogens
Band
11
Ausgabe
1
Art.-Nr.
e1004600
eISSN
1553-7374
Page URI
https://pub.uni-bielefeld.de/record/2953303

Zitieren

Avican K, Fahlgren A, Huss M, et al. Reprogramming of Yersinia from Virulent to Persistent Mode Revealed by Complex In Vivo RNA-seq Analysis. PLOS Pathogens. 2015;11(1): e1004600.
Avican, K., Fahlgren, A., Huss, M., Heroven, A. K., Beckstette, M., Dersch, P., & Fällman, M. (2015). Reprogramming of Yersinia from Virulent to Persistent Mode Revealed by Complex In Vivo RNA-seq Analysis. PLOS Pathogens, 11(1), e1004600. https://doi.org/10.1371/journal.ppat.1004600
Avican, K., Fahlgren, A., Huss, M., Heroven, A. K., Beckstette, M., Dersch, P., and Fällman, M. (2015). Reprogramming of Yersinia from Virulent to Persistent Mode Revealed by Complex In Vivo RNA-seq Analysis. PLOS Pathogens 11:e1004600.
Avican, K., et al., 2015. Reprogramming of Yersinia from Virulent to Persistent Mode Revealed by Complex In Vivo RNA-seq Analysis. PLOS Pathogens, 11(1): e1004600.
K. Avican, et al., “Reprogramming of Yersinia from Virulent to Persistent Mode Revealed by Complex In Vivo RNA-seq Analysis”, PLOS Pathogens, vol. 11, 2015, : e1004600.
Avican, K., Fahlgren, A., Huss, M., Heroven, A.K., Beckstette, M., Dersch, P., Fällman, M.: Reprogramming of Yersinia from Virulent to Persistent Mode Revealed by Complex In Vivo RNA-seq Analysis. PLOS Pathogens. 11, : e1004600 (2015).
Avican, Kemal, Fahlgren, Anna, Huss, Mikael, Heroven, Ann Kathrin, Beckstette, Michael, Dersch, Petra, and Fällman, Maria. “Reprogramming of Yersinia from Virulent to Persistent Mode Revealed by Complex In Vivo RNA-seq Analysis”. PLOS Pathogens 11.1 (2015): e1004600.

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 25590628
PubMed | Europe PMC

Suchen in

Google Scholar