Tracking gene expression and oxidative damage of O2-stressed Clostridioides difficile by a multi-omics approach

Neumann-Schaal M, Metzendorf NG, Troitzsch D, Nuss AM, Hofmann JD, Beckstette M, Dersch P, Otto A, Sievers S (2018)
Anaerobe 53: 94-107.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Autor*in
Neumann-Schaal, Meina; Metzendorf, Nicole G.; Troitzsch, Daniel; Nuss, Aaron Mischa; Hofmann, Julia Danielle; Beckstette, MichaelUniBi; Dersch, Petra; Otto, Andreas; Sievers, Susanne
Abstract / Bemerkung
Clostridioides difficile is the major pathogen causing diarrhea following antibiotic treatment. It is considered to be a strictly anaerobic bacterium, however, previous studies have shown a certain and strain-dependent oxygen tolerance. In this study, the model strain C. difficile 630Δerm was shifted to micro-aerobiosis and was found to stay growing to the same extent as anaerobically growing cells with only few changes in the metabolite pattern. However, an extensive change in gene expression was determined by RNA-Seq. The most striking adaptation strategies involve a change in the reductive fermentation pathways of the amino acids proline, glycine and leucine. But also a far-reaching restructuring in the carbohydrate metabolism was detected with changes in the phosphotransferase system (PTS) facilitated uptake of sugars and a repression of enzymes of glycolysis and butyrate fermentation. Furthermore, a temporary induction in the synthesis of cofactor riboflavin was detected possibly due to an increased demand for flavin mononucleotid (FMN) and flavin adenine dinucleotide (FAD) in redox reactions. However, biosynthesis of the cofactors thiamin pyrophosphate and cobalamin were repressed deducing oxidation-prone enzymes and intermediates in these pathways. Micro-aerobically shocked cells were characterized by an increased demand for cysteine and a thiol redox proteomics approach revealed a dramatic increase in the oxidative state of cysteine in more than 800 peptides after 15 min of micro-aerobic shock. This provides not only a catalogue of oxidation-prone cysteine residues in the C. difficile proteome but also puts the amino acid cysteine into a key position in the oxidative stress response. Our study suggests that tolerance of C. difficile towards O2 is based on a complex and far-reaching adjustment of global gene expression which leads to only a slight change in phenotype.
Erscheinungsjahr
2018
Zeitschriftentitel
Anaerobe
Band
53
Seite(n)
94-107
ISSN
10759964
Page URI
https://pub.uni-bielefeld.de/record/2953291

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Neumann-Schaal M, Metzendorf NG, Troitzsch D, et al. Tracking gene expression and oxidative damage of O2-stressed Clostridioides difficile by a multi-omics approach. Anaerobe. 2018;53:94-107.
Neumann-Schaal, M., Metzendorf, N. G., Troitzsch, D., Nuss, A. M., Hofmann, J. D., Beckstette, M., Dersch, P., et al. (2018). Tracking gene expression and oxidative damage of O2-stressed Clostridioides difficile by a multi-omics approach. Anaerobe, 53, 94-107. https://doi.org/10.1016/j.anaerobe.2018.05.018
Neumann-Schaal, M., Metzendorf, N. G., Troitzsch, D., Nuss, A. M., Hofmann, J. D., Beckstette, M., Dersch, P., Otto, A., and Sievers, S. (2018). Tracking gene expression and oxidative damage of O2-stressed Clostridioides difficile by a multi-omics approach. Anaerobe 53, 94-107.
Neumann-Schaal, M., et al., 2018. Tracking gene expression and oxidative damage of O2-stressed Clostridioides difficile by a multi-omics approach. Anaerobe, 53, p 94-107.
M. Neumann-Schaal, et al., “Tracking gene expression and oxidative damage of O2-stressed Clostridioides difficile by a multi-omics approach”, Anaerobe, vol. 53, 2018, pp. 94-107.
Neumann-Schaal, M., Metzendorf, N.G., Troitzsch, D., Nuss, A.M., Hofmann, J.D., Beckstette, M., Dersch, P., Otto, A., Sievers, S.: Tracking gene expression and oxidative damage of O2-stressed Clostridioides difficile by a multi-omics approach. Anaerobe. 53, 94-107 (2018).
Neumann-Schaal, Meina, Metzendorf, Nicole G., Troitzsch, Daniel, Nuss, Aaron Mischa, Hofmann, Julia Danielle, Beckstette, Michael, Dersch, Petra, Otto, Andreas, and Sievers, Susanne. “Tracking gene expression and oxidative damage of O2-stressed Clostridioides difficile by a multi-omics approach”. Anaerobe 53 (2018): 94-107.

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