Nanopore Sequencing Reveals Global Transcriptome Signatures of Mitochondrial and Ribosomal Gene Expressions in Various Human Cancer Stem-like Cell Populations

Witte KE, Hertel O, Windmöller BA, Helweg LP, Höving AL, Knabbe C, Busche T, Greiner J, Kalinowski J, Noll T, Mertzlufft F, et al. (2021)
Cancers 13(5): 1136.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Witte, Kaya E.; Hertel, OliverUniBi ; Windmöller, Beatrice A.; Helweg, Laureen P.; Höving, Anna L.; Knabbe, CorneliusUniBi; Busche, TobiasUniBi; Greiner, JohannesUniBi ; Kalinowski, JörnUniBi; Noll, ThomasUniBi ; Mertzlufft, Fritz; Beshay, Morris
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Abstract / Bemerkung
Cancer stem cells (CSCs) are crucial mediators of tumor growth, metastasis, therapy resistance, and recurrence in a broad variety of human cancers. Although their biology is increasingly investigated within the distinct types of cancer, direct comparisons of CSCs from different tumor types allowing comprehensive mechanistic insights are rarely assessed. In the present study, we isolated CSCs from endometrioid carcinomas, glioblastoma multiforme as well as adenocarcinomas of lung and prostate and assessed their global transcriptomes using full-length cDNA nanopore sequencing. Despite the expression of common CSC markers, principal component analysis showed a distinct separation of the CSC populations into three clusters independent of the specific type of tumor. However, GO-term and KEGG pathway enrichment analysis revealed upregulated genes related to ribosomal biosynthesis, the mitochondrion, oxidative phosphorylation, and glycolytic pathways, as well as the proteasome, suggesting a great extent of metabolic flexibility in CSCs. Interestingly, the GO term “NF-kB binding” was likewise found to be elevated in all investigated CSC populations. In summary, we here provide evidence for high global transcriptional similarities between CSCs from various tumors, which particularly share upregulated gene expression associated with mitochondrial and ribosomal activity. Our findings may build the basis for identifying novel therapeutic strategies targeting CSCs
Erscheinungsjahr
2021
Zeitschriftentitel
Cancers
Band
13
Ausgabe
5
Art.-Nr.
1136
eISSN
2072-6694
Page URI
https://pub.uni-bielefeld.de/record/2952449

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Witte KE, Hertel O, Windmöller BA, et al. Nanopore Sequencing Reveals Global Transcriptome Signatures of Mitochondrial and Ribosomal Gene Expressions in Various Human Cancer Stem-like Cell Populations. Cancers. 2021;13(5): 1136.
Witte, K. E., Hertel, O., Windmöller, B. A., Helweg, L. P., Höving, A. L., Knabbe, C., Busche, T., et al. (2021). Nanopore Sequencing Reveals Global Transcriptome Signatures of Mitochondrial and Ribosomal Gene Expressions in Various Human Cancer Stem-like Cell Populations. Cancers, 13(5), 1136. https://doi.org/10.3390/cancers13051136
Witte, K. E., Hertel, O., Windmöller, B. A., Helweg, L. P., Höving, A. L., Knabbe, C., Busche, T., Greiner, J., Kalinowski, J., Noll, T., et al. (2021). Nanopore Sequencing Reveals Global Transcriptome Signatures of Mitochondrial and Ribosomal Gene Expressions in Various Human Cancer Stem-like Cell Populations. Cancers 13:1136.
Witte, K.E., et al., 2021. Nanopore Sequencing Reveals Global Transcriptome Signatures of Mitochondrial and Ribosomal Gene Expressions in Various Human Cancer Stem-like Cell Populations. Cancers, 13(5): 1136.
K.E. Witte, et al., “Nanopore Sequencing Reveals Global Transcriptome Signatures of Mitochondrial and Ribosomal Gene Expressions in Various Human Cancer Stem-like Cell Populations”, Cancers, vol. 13, 2021, : 1136.
Witte, K.E., Hertel, O., Windmöller, B.A., Helweg, L.P., Höving, A.L., Knabbe, C., Busche, T., Greiner, J., Kalinowski, J., Noll, T., Mertzlufft, F., Beshay, M., Pfitzenmaier, J., Kaltschmidt, B., Kaltschmidt, C., Banz-Jansen, C., Simonis, M.: Nanopore Sequencing Reveals Global Transcriptome Signatures of Mitochondrial and Ribosomal Gene Expressions in Various Human Cancer Stem-like Cell Populations. Cancers. 13, : 1136 (2021).
Witte, Kaya E., Hertel, Oliver, Windmöller, Beatrice A., Helweg, Laureen P., Höving, Anna L., Knabbe, Cornelius, Busche, Tobias, Greiner, Johannes, Kalinowski, Jörn, Noll, Thomas, Mertzlufft, Fritz, Beshay, Morris, Pfitzenmaier, Jesco, Kaltschmidt, Barbara, Kaltschmidt, Christian, Banz-Jansen, Constanze, and Simonis, Matthias. “Nanopore Sequencing Reveals Global Transcriptome Signatures of Mitochondrial and Ribosomal Gene Expressions in Various Human Cancer Stem-like Cell Populations”. Cancers 13.5 (2021): 1136.
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2021-03-22T10:17:45Z
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