pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110

Schaffert, Lena; Jacob, Lucas; Schneiker-Bekel, Susanne; Persicke, Marcus; März, Camilla; Rückert, Christian; Pühler, Alfred; Kalinowski, Jörn

pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110

Schaffert L, Jacob L, Schneiker-Bekel S, Persicke M, März C, Rückert C, Pühler A, Kalinowski J (2020)
Microbiology Resource Announcements 9(39): e00596-20.

Zeitschriftenaufsatz | Veröffentlicht | Englisch

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URL

https://mra.asm.org/content/9/39/e00596-20

DOI

https://doi.org/10.1128/mra.00596-20

URN

urn:nbn:de:0070-pub-29461574

Autor*in

Schaffert, Lena^UniBi ; Jacob, Lucas^UniBi; Schneiker-Bekel, Susanne^UniBi; Persicke, Marcus^UniBi; März, Camilla; Rückert, Christian^UniBi ; Pühler, Alfred^UniBi ; Kalinowski, Jörn^UniBi

Einrichtung

Centrum für Biotechnologie > Graduate Center > Graduate Cluster Industrial Biotechnology

Abstract / Bemerkung

ABSTRACT The pSETT4 vector integrates into the Actinoplanes sp. SE50/110 chromosome via the bacteriophage φC31 integrase and allows cloning of a gene of interest by Golden Gate assembly (BsaI). T4 terminators surround the expression cassette to isolate the transcriptional unit and to prevent antisense transcription. The system can be used in other Actinomycetales by exchanging the promoter.

Erscheinungsjahr

2020

Zeitschriftentitel

Microbiology Resource Announcements

Band

Ausgabe

Art.-Nr.

e00596-20

Urheberrecht / Lizenzen

Creative Commons Namensnennung - Weitergabe unter gleichen Bedingungen 4.0 International Public License (CC BY-SA 4.0)

eISSN

2576-098X

Page URI

https://pub.uni-bielefeld.de/record/2946157

Zitieren

Schaffert L, Jacob L, Schneiker-Bekel S, et al. pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110. Microbiology Resource Announcements. 2020;9(39): e00596-20.

Schaffert, L., Jacob, L., Schneiker-Bekel, S., Persicke, M., März, C., Rückert, C., Pühler, A., et al. (2020). pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110. Microbiology Resource Announcements, 9(39), e00596-20. doi:10.1128/mra.00596-20

Schaffert, Lena, Jacob, Lucas, Schneiker-Bekel, Susanne, Persicke, Marcus, März, Camilla, Rückert, Christian, Pühler, Alfred, and Kalinowski, Jörn. 2020. “pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110”. Microbiology Resource Announcements 9 (39): e00596-20.

Schaffert, L., Jacob, L., Schneiker-Bekel, S., Persicke, M., März, C., Rückert, C., Pühler, A., and Kalinowski, J. (2020). pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110. Microbiology Resource Announcements 9:e00596-20.

Schaffert, L., et al., 2020. pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110. Microbiology Resource Announcements, 9(39): e00596-20.

L. Schaffert, et al., “pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110”, Microbiology Resource Announcements, vol. 9, 2020, : e00596-20.

Schaffert, L., Jacob, L., Schneiker-Bekel, S., Persicke, M., März, C., Rückert, C., Pühler, A., Kalinowski, J.: pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110. Microbiology Resource Announcements. 9, : e00596-20 (2020).

Schaffert, Lena, Jacob, Lucas, Schneiker-Bekel, Susanne, Persicke, Marcus, März, Camilla, Rückert, Christian, Pühler, Alfred, and Kalinowski, Jörn. “pSETT4, an Improved φC31-Based Integrative Vector System for Actinoplanes sp. SE50/110”. Microbiology Resource Announcements 9.39 (2020): e00596-20.

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