The Peptidoglycan Biosynthesis Gene murC in Frankia: Actinorhizal vs. Plant Type.

Berckx F, Wibberg D, Kalinowski J, Pawlowski K (2020)
Genes 11(4).

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
Download
OA 1.41 MB
Autor*in
Berckx, Fede; Wibberg, DanielUniBi; Kalinowski, JörnUniBi; Pawlowski, Katharina
Abstract / Bemerkung
Nitrogen-fixing Actinobacteria of the genus Frankia can be subdivided into four phylogenetically distinct clades; members of clusters one to three engage in nitrogen-fixing root nodule symbioses with actinorhizal plants. Mur enzymes are responsible for the biosynthesis of the peptidoglycan layer of bacteria. The four Mur ligases, MurC, MurD, MurE, and MurF, catalyse the addition of a short polypeptide to UDP-N-acetylmuramic acid. Frankia strains of cluster-2 and cluster-3 contain two copies of murC, while the strains of cluster-1 and cluster-4 contain only one. Phylogenetically, the protein encoded by the murC gene shared only by cluster-2 and cluster-3, termed MurC1, groups with MurC proteins of other Actinobacteria. The protein encoded by the murC gene found in all Frankia strains, MurC2, shows a higher similarity to the MurC proteins of plants than of Actinobacteria. MurC2 could have been either acquired via horizontal gene transfer or via gene duplication and convergent evolution, while murC1 was subsequently lost in the cluster-1 and cluster-4 strains. In the nodules induced by the cluster-2 strains, the expression levels of murC2 were significantly higher than those of murC1. Thus, there is clear sequence divergence between both types of Frankia MurC, and Frankia murC1 is in the process of being replaced by murC2, indicating selection in favour of murC2. Nevertheless, protein modelling showed no major structural differences between the MurCs from any phylogenetic group examined.
Erscheinungsjahr
2020
Zeitschriftentitel
Genes
Band
11
Ausgabe
4
eISSN
2073-4425
Page URI
https://pub.uni-bielefeld.de/record/2943125

Zitieren

Berckx F, Wibberg D, Kalinowski J, Pawlowski K. The Peptidoglycan Biosynthesis Gene murC in Frankia: Actinorhizal vs. Plant Type. Genes. 2020;11(4).
Berckx, F., Wibberg, D., Kalinowski, J., & Pawlowski, K. (2020). The Peptidoglycan Biosynthesis Gene murC in Frankia: Actinorhizal vs. Plant Type. Genes, 11(4). doi:10.3390/genes11040432
Berckx, Fede, Wibberg, Daniel, Kalinowski, Jörn, and Pawlowski, Katharina. 2020. “The Peptidoglycan Biosynthesis Gene murC in Frankia: Actinorhizal vs. Plant Type.”. Genes 11 (4).
Berckx, F., Wibberg, D., Kalinowski, J., and Pawlowski, K. (2020). The Peptidoglycan Biosynthesis Gene murC in Frankia: Actinorhizal vs. Plant Type. Genes 11.
Berckx, F., et al., 2020. The Peptidoglycan Biosynthesis Gene murC in Frankia: Actinorhizal vs. Plant Type. Genes, 11(4).
F. Berckx, et al., “The Peptidoglycan Biosynthesis Gene murC in Frankia: Actinorhizal vs. Plant Type.”, Genes, vol. 11, 2020.
Berckx, F., Wibberg, D., Kalinowski, J., Pawlowski, K.: The Peptidoglycan Biosynthesis Gene murC in Frankia: Actinorhizal vs. Plant Type. Genes. 11, (2020).
Berckx, Fede, Wibberg, Daniel, Kalinowski, Jörn, and Pawlowski, Katharina. “The Peptidoglycan Biosynthesis Gene murC in Frankia: Actinorhizal vs. Plant Type.”. Genes 11.4 (2020).
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Creative Commons Namensnennung 4.0 International Public License (CC-BY 4.0):
Volltext(e)
Access Level
OA Open Access
Zuletzt Hochgeladen
2020-05-19T08:31:16Z
MD5 Prüfsumme
6fb58742d2960f6c8237c6d33c42deae


Link(s) zu Volltext(en)
Access Level
OA Open Access

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®
Quellen

PMID: 32316316
PubMed | Europe PMC

Suchen in

Google Scholar