Twenty-Five Years of Propagation in Suspension Cell Culture Results in Substantial Alterations of the Arabidopsis Thaliana Genome

Pucker B, Rückert C, Stracke R, Viehöver P, Kalinowski J, Weisshaar B (2019)
Genes 10(9): 671.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Arabidopsis thaliana is one of the best studied plant model organisms. Besides cultivation in greenhouses, cells of this plant can also be propagated in suspension cell culture. At7 is one such cell line that was established about 25 years ago. Here, we report the sequencing and the analysis of the At7 genome. Large scale duplications and deletions compared to the Columbia-0 (Col-0) reference sequence were detected. The number of deletions exceeds the number of insertions, thus indicating that a haploid genome size reduction is ongoing. Patterns of small sequence variants differ from the ones observed between A. thaliana accessions, e.g., the number of single nucleotide variants matches the number of insertions/deletions. RNA-Seq analysis reveals that disrupted alleles are less frequent in the transcriptome than the native ones.
Stichworte
copy number variations; variant calling; next generation sequencing; long read sequencing
Erscheinungsjahr
2019
Zeitschriftentitel
Genes
Band
10
Ausgabe
9
Art.-Nr.
671
eISSN
2073-4425
Finanzierungs-Informationen
Article Processing Charge funded by the Deutsche Forschungsgemeinschaft and the Open Access Publication Fund of Bielefeld University.
Page URI
https://pub.uni-bielefeld.de/record/2937135

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Pucker B, Rückert C, Stracke R, Viehöver P, Kalinowski J, Weisshaar B. Twenty-Five Years of Propagation in Suspension Cell Culture Results in Substantial Alterations of the Arabidopsis Thaliana Genome. Genes. 2019;10(9): 671.
Pucker, B., Rückert, C., Stracke, R., Viehöver, P., Kalinowski, J., & Weisshaar, B. (2019). Twenty-Five Years of Propagation in Suspension Cell Culture Results in Substantial Alterations of the Arabidopsis Thaliana Genome. Genes, 10(9), 671. doi:10.3390/genes10090671
Pucker, B., Rückert, C., Stracke, R., Viehöver, P., Kalinowski, J., and Weisshaar, B. (2019). Twenty-Five Years of Propagation in Suspension Cell Culture Results in Substantial Alterations of the Arabidopsis Thaliana Genome. Genes 10:671.
Pucker, B., et al., 2019. Twenty-Five Years of Propagation in Suspension Cell Culture Results in Substantial Alterations of the Arabidopsis Thaliana Genome. Genes, 10(9): 671.
B. Pucker, et al., “Twenty-Five Years of Propagation in Suspension Cell Culture Results in Substantial Alterations of the Arabidopsis Thaliana Genome”, Genes, vol. 10, 2019, : 671.
Pucker, B., Rückert, C., Stracke, R., Viehöver, P., Kalinowski, J., Weisshaar, B.: Twenty-Five Years of Propagation in Suspension Cell Culture Results in Substantial Alterations of the Arabidopsis Thaliana Genome. Genes. 10, : 671 (2019).
Pucker, Boas, Rückert, Christian, Stracke, Ralf, Viehöver, Prisca, Kalinowski, Jörn, and Weisshaar, Bernd. “Twenty-Five Years of Propagation in Suspension Cell Culture Results in Substantial Alterations of the Arabidopsis Thaliana Genome”. Genes 10.9 (2019): 671.
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2019-09-03T09:25:11Z
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