Comparative transcription profiling of two fermentation cultures of Xanthomonas campestris pv. campestris B100 sampled in the growth and in the stationary phase

Alkhateeb R, Vorhölter F-J, Steffens T, Rückert C, Ortseifen V, Hublik G, Niehaus K, Pühler A (2018)
Applied Microbiology and Biotechnology 102(15): 6613-6625.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
The E -proteobacterium pound Xanthomonas campestris pv. campestris (Xcc) is the producer of the biopolymer xanthan, a polysaccharide which is used as a thickener in numerous industrial applications. In this study, we present a global transcriptome profiling of two Xcc strain B100 cultures obtained from fermentation during the growth phase and the subsequent stationary phase associated with xanthan biosynthesis. During the xanthan production phase, highly abundant transcripts belonged to genes encoding for small RNAs, glycogen biosynthesis, and xanthan export. A total of 1850 (40%) genes were differentially transcribed during the stationary phase where 924 were transcriptionally up-regulated and 926 genes were down-regulated. An overview of differentially transcribed genes includes a significant down-regulation of genes involved in transcription, translation, and amino acid biosynthesis pathways. A group of up-regulated genes was involved in cellular response against oxidative stress, such as those coding for superoxide dismutase and catalase. Genes encoding enzymes involved in nucleotide sugar precursor synthesis of xanthan biosynthesis, such as xanA, galU, and ugd, exhibited a transcription pattern that did not change during the growth and stationary phase. Regarding the transcription pattern of the gum gene cluster that govern xanthan biosynthesis, a significant up-regulation of the genes gumB, gumC, and gumD was observed, while the transcript pools of the genes gumG, gumH, gumI, and gumJ were reduced and those of genes gumE, gumF, gumK, gumL, and gumM remained un-changed during the stationary phase compared to the growth phase. The obtained data represents the first analysis of gene expression patterns under xanthan production conditions and provides the bases for future studies aiming at enhancing xanthan yield.
Stichworte
Xanthan biosynthesis; RNA sequencing; Differential transcriptomics; Xanthomonas; Plant pathogen
Erscheinungsjahr
2018
Zeitschriftentitel
Applied Microbiology and Biotechnology
Band
102
Ausgabe
15
Seite(n)
6613-6625
ISSN
0175-7598
eISSN
1432-0614
Page URI
https://pub.uni-bielefeld.de/record/2930510

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Alkhateeb R, Vorhölter F-J, Steffens T, et al. Comparative transcription profiling of two fermentation cultures of Xanthomonas campestris pv. campestris B100 sampled in the growth and in the stationary phase. Applied Microbiology and Biotechnology. 2018;102(15):6613-6625.
Alkhateeb, R., Vorhölter, F. - J., Steffens, T., Rückert, C., Ortseifen, V., Hublik, G., Niehaus, K., et al. (2018). Comparative transcription profiling of two fermentation cultures of Xanthomonas campestris pv. campestris B100 sampled in the growth and in the stationary phase. Applied Microbiology and Biotechnology, 102(15), 6613-6625. https://doi.org/10.1007/s00253-018-9106-2
Alkhateeb, Rabeaa, Vorhölter, Frank-Jörg, Steffens, Tim, Rückert, Christian, Ortseifen, Vera, Hublik, Gerd, Niehaus, Karsten, and Pühler, Alfred. 2018. “Comparative transcription profiling of two fermentation cultures of Xanthomonas campestris pv. campestris B100 sampled in the growth and in the stationary phase”. Applied Microbiology and Biotechnology 102 (15): 6613-6625.
Alkhateeb, R., Vorhölter, F. - J., Steffens, T., Rückert, C., Ortseifen, V., Hublik, G., Niehaus, K., and Pühler, A. (2018). Comparative transcription profiling of two fermentation cultures of Xanthomonas campestris pv. campestris B100 sampled in the growth and in the stationary phase. Applied Microbiology and Biotechnology 102, 6613-6625.
Alkhateeb, R., et al., 2018. Comparative transcription profiling of two fermentation cultures of Xanthomonas campestris pv. campestris B100 sampled in the growth and in the stationary phase. Applied Microbiology and Biotechnology, 102(15), p 6613-6625.
R. Alkhateeb, et al., “Comparative transcription profiling of two fermentation cultures of Xanthomonas campestris pv. campestris B100 sampled in the growth and in the stationary phase”, Applied Microbiology and Biotechnology, vol. 102, 2018, pp. 6613-6625.
Alkhateeb, R., Vorhölter, F.-J., Steffens, T., Rückert, C., Ortseifen, V., Hublik, G., Niehaus, K., Pühler, A.: Comparative transcription profiling of two fermentation cultures of Xanthomonas campestris pv. campestris B100 sampled in the growth and in the stationary phase. Applied Microbiology and Biotechnology. 102, 6613-6625 (2018).
Alkhateeb, Rabeaa, Vorhölter, Frank-Jörg, Steffens, Tim, Rückert, Christian, Ortseifen, Vera, Hublik, Gerd, Niehaus, Karsten, and Pühler, Alfred. “Comparative transcription profiling of two fermentation cultures of Xanthomonas campestris pv. campestris B100 sampled in the growth and in the stationary phase”. Applied Microbiology and Biotechnology 102.15 (2018): 6613-6625.

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