DMPC vesicle structure and dynamics in the presence of low amounts of the saponin aescin

Sreij R, Dargel C, Geisler P, Hertle Y, Radulescu A, Pasini S, Perez J, Hernandez Moleiro L, Hellweg T (2018)
PHYSICAL CHEMISTRY CHEMICAL PHYSICS 20(14): 9070-9083.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Vesicle shape and bilayer parameters are studied by small-angle X-ray (SAXS) and small-angle neutron (SANS) scattering in the presence of the saponin aescin. We confirm successful incorporation of aescin molecules by analysis of the radii of gyration RG and study furthermore the impact of aescin incorporation on bilayer thickness parameters from the neutron and X-ray perspective. Additionally, the bending elasticity (k) of these 1,2-dimyristoyl-sn-glycero-3-phosphocholine vesicle bilayers is studied in the presence of aescin. Neutron spin-echo spectroscopy (NSE) allows to detect subtle changes in the dynamics and k of lipid membranes. Changes of k are detectable at temperatures below and above the main phase transition temperature Tm of the lipid. The impact of aescin is much more significant below Tm. It has been found that below Tm the addition of aescin to the vesicles decreases the value of k and softens the bilayer. Above Tm the value of k increases with increasing aescin content and the bilayer becomes more rigid. Altogether, we demonstrate by analysis of the structure and dynamics of the vesicles that the impact of aescin strongly depends on the lipid state. Below Tm the membrane becomes fluidized and softer, above Tm solidified and stiffer compared to a DMPC membrane without additive at similar conditions.
Erscheinungsjahr
2018
Zeitschriftentitel
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
Band
20
Ausgabe
14
Seite(n)
9070-9083
ISSN
1463-9076
eISSN
1463-9084
Page URI
https://pub.uni-bielefeld.de/record/2919290

Zitieren

Sreij R, Dargel C, Geisler P, et al. DMPC vesicle structure and dynamics in the presence of low amounts of the saponin aescin. PHYSICAL CHEMISTRY CHEMICAL PHYSICS. 2018;20(14):9070-9083.
Sreij, R., Dargel, C., Geisler, P., Hertle, Y., Radulescu, A., Pasini, S., Perez, J., et al. (2018). DMPC vesicle structure and dynamics in the presence of low amounts of the saponin aescin. PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 20(14), 9070-9083. doi:10.1039/c7cp08027a
Sreij, Ramsia, Dargel, Carina, Geisler, Philippe, Hertle, Yvonne, Radulescu, Aurel, Pasini, Stefano, Perez, Javier, Hernandez Moleiro, Lara, and Hellweg, Thomas. 2018. “DMPC vesicle structure and dynamics in the presence of low amounts of the saponin aescin”. PHYSICAL CHEMISTRY CHEMICAL PHYSICS 20 (14): 9070-9083.
Sreij, R., Dargel, C., Geisler, P., Hertle, Y., Radulescu, A., Pasini, S., Perez, J., Hernandez Moleiro, L., and Hellweg, T. (2018). DMPC vesicle structure and dynamics in the presence of low amounts of the saponin aescin. PHYSICAL CHEMISTRY CHEMICAL PHYSICS 20, 9070-9083.
Sreij, R., et al., 2018. DMPC vesicle structure and dynamics in the presence of low amounts of the saponin aescin. PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 20(14), p 9070-9083.
R. Sreij, et al., “DMPC vesicle structure and dynamics in the presence of low amounts of the saponin aescin”, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, vol. 20, 2018, pp. 9070-9083.
Sreij, R., Dargel, C., Geisler, P., Hertle, Y., Radulescu, A., Pasini, S., Perez, J., Hernandez Moleiro, L., Hellweg, T.: DMPC vesicle structure and dynamics in the presence of low amounts of the saponin aescin. PHYSICAL CHEMISTRY CHEMICAL PHYSICS. 20, 9070-9083 (2018).
Sreij, Ramsia, Dargel, Carina, Geisler, Philippe, Hertle, Yvonne, Radulescu, Aurel, Pasini, Stefano, Perez, Javier, Hernandez Moleiro, Lara, and Hellweg, Thomas. “DMPC vesicle structure and dynamics in the presence of low amounts of the saponin aescin”. PHYSICAL CHEMISTRY CHEMICAL PHYSICS 20.14 (2018): 9070-9083.

1 Zitation in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Aescin-Cholesterol Complexes in DMPC Model Membranes: A DSC and Temperature-Dependent Scattering Study.
Sreij R, Dargel C, Schweins R, Prévost S, Dattani R, Hellweg T., Sci Rep 9(1), 2019
PMID: 30944386

72 References

Daten bereitgestellt von Europe PubMed Central.

Molecular activities, biosynthesis and evolution of triterpenoid saponins.
Augustin JM, Kuzina V, Andersen SB, Bak S., Phytochemistry 72(6), 2011
PMID: 21333312
Complexation of phospholipids and cholesterol by triterpenic saponins in bulk and in monolayers.
Wojciechowski K, Orczyk M, Gutberlet T, Geue T., Biochim. Biophys. Acta 1858(2), 2015
PMID: 26654784
Unusual penetration of phospholipid mono- and bilayers by Quillaja bark saponin biosurfactant.
Wojciechowski K, Orczyk M, Gutberlet T, Trapp M, Marcinkowski K, Kobiela T, Geue T., Biochim. Biophys. Acta 1838(7), 2014
PMID: 24746451
Exploration of the correlation between the structure, hemolytic activity, and cytotoxicity of steroid saponins.
Wang Y, Zhang Y, Zhu Z, Zhu S, Li Y, Li M, Yu B., Bioorg. Med. Chem. 15(7), 2007
PMID: 17306549
Triterpenoid saponins from Quillaja saponaria.
Guo S, Kenne L, Lundgren LN, Ronnberg B, Sundquist BG., Phytochemistry 48(1), 1998
PMID: 9621458
The role of the hydrophobic phase in the unique rheological properties of saponin adsorption layers.
Golemanov K, Tcholakova S, Denkov N, Pelan E, Stoyanov SD., Soft Matter 10(36), 2014
PMID: 24945943
Escin inhibits type I allergic dermatitis in a novel porcine model.
Sipos W, Reutterer B, Frank M, Unger H, Grassauer A, Prieschl-Grassauer E, Doerfler P., Int. Arch. Allergy Immunol. 161(1), 2012
PMID: 23257653
Aescin Incorporation and Nanodomain Formation in DMPC Model Membranes.
Sreij R, Dargel C, Moleiro LH, Monroy F, Hellweg T., Langmuir 33(43), 2017
PMID: 28985678
Aescin: pharmacology, pharmacokinetics and therapeutic profile.
Sirtori CR., Pharmacol. Res. 44(3), 2001
PMID: 11529685
Surface activity of saponin from Quillaja bark at the air/water and oil/water interfaces.
Wojciechowski K., Colloids Surf B Biointerfaces 108(), 2013
PMID: 23524082

Janiak, J. Biophys. Chem. 254(), 1979
Small-angle neutron scattering from large unilamellar vesicles: an improved method for membrane thickness determination.
Pencer J, Hallett FR., Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics 61(3), 2000
PMID: 11046629
Bilayer thickness and lipid interface area in unilamellar extruded 1,2-diacylphosphatidylcholine liposomes: a small-angle neutron scattering study.
Balgavy P, Dubnickova M, Kucerka N, Kiselev MA, Yaradaikin SP, Uhrikova D., Biochim. Biophys. Acta 1512(1), 2001
PMID: 11334623
Does sucrose influence the properties of DMPC vesicles?
Kiselev MA, Wartewig S, Janich M, Lesieur P, Kiselev AM, Ollivon M, Neubert R., Chem. Phys. Lipids 123(1), 2003
PMID: 12637163
Synthetic phospholipid analogs: a structural investigation with scattering methods.
Orthaber D, Glatter O., Chem. Phys. Lipids 107(2), 2000
PMID: 11090847
Undulations and Dynamic Structure Factor of Membranes.
Zilman AG, Granek R., Phys. Rev. Lett. 77(23), 1996
PMID: 10062631

Zilman, Chem. Phys. 284(), 2002

Bergmann, J. Appl. Crystallogr. 33(), 2000

Mittelbach, J. Appl. Crystallogr. 31(), 1998
Diffuse small-angle scattering of X-rays in colloid systems.
KRATKY O, POROD G., J Colloid Sci 4(1), 1949
PMID: 18110601

Glatter, J. Appl. Crystallogr. 13(), 1980

Zhang, Metall. Mater. Trans. A 41(), 2010

Radulescu, Journal of large-scale research facilites 1(), 2015
Fluctuation dynamics of spherical vesicles: frustration of regular bulk dissipation into subdiffusive relaxation.
Arriaga LR, Lopez-Montero I, Orts-Gil G, Farago B, Hellweg T, Monroy F., Phys Rev E Stat Nonlin Soft Matter Phys 80(3 Pt 1), 2009
PMID: 19905147

Helfrich, Z. Naturforsch. 28c(), 1973
Dynamical fluctuations of droplet microemulsions and vesicles.
Milner ST, Safran SA., Phys Rev A Gen Phys 36(9), 1987
PMID: 9899393

Hellweg, Phys. Chem. Chem. Phys. 2(), 2000

Hellweg, Colloids Surf., A 183–185(), 2001
Vesicle fluctuation analysis of the effects of sterols on membrane bending rigidity.
Henriksen J, Rowat AC, Ipsen JH., Eur. Biophys. J. 33(8), 2004
PMID: 15221234
Dissipative curvature fluctuations in bilayer vesicles: Coexistence of pure-bending and hybrid curvature-compression modes.
Arriaga LR, Rodriguez-Garcia R, Lopez-Montero I, Farago B, Hellweg T, Monroy F., Eur Phys J E Soft Matter 31(1), 2010
PMID: 20087620
Lipid bilayers and membrane dynamics: insight into thickness fluctuations.
Woodka AC, Butler PD, Porcar L, Farago B, Nagao M., Phys. Rev. Lett. 109(5), 2012
PMID: 23006210

Marignan, J. Phys. 50(), 1989

Gazeau, Europhys. Lett. 9(), 1989

Van, Phys. Rev. 95(), 1954

Holderer, Meas. Sci. Technol. 19(), 2008

Berne, 2000

Provencher, Comput. Phys. Commun. 27(), 1982

Guinier, 1955

Knoll, J. Appl. Crystallogr. 14(), 1981

Glatter, 0
Areas of monounsaturated diacylphosphatidylcholines.
Kucerka N, Gallova J, Uhrikova D, Balgavy P, Bulacu M, Marrink SJ, Katsaras J., Biophys. J. 97(7), 2009
PMID: 19804723
Analysis of Quil A-phospholipid mixtures using drift spectroscopy.
Demana PH, Davies NM, Hook S, Rades T., Int J Pharm 342(1-2), 2007
PMID: 17555894
The membrane-activity of Ibuprofen, Diclofenac, and Naproxen: a physico-chemical study with lecithin phospholipids.
Manrique-Moreno M, Moreno MM, Garidel P, Suwalsky M, Howe J, Brandenburg K., Biochim. Biophys. Acta 1788(6), 2009
PMID: 19366589
Action of saponin on biological cell membranes.
BANGHAM AD, HORNE RW, GLAUERT AM, DINGLE JT, LUCY JA., Nature 196(), 1962
PMID: 13966357
Electron microscopic observations on Rous sarcoma virus and cell membranes.
DOURMASHKIN RR, DOUGHERTY RM, HARRIS RJ., Nature 194(), 1962
PMID: 13887581
Self-Assembly of Escin Molecules at the Air-Water Interface as Studied by Molecular Dynamics.
Tsibranska S, Ivanova A, Tcholakova S, Denkov N., Langmuir 33(33), 2017
PMID: 28749143
Small-angle neutron scattering studies of phospholipid-NSAID adducts.
Boggara MB, Krishnamoorti R., Langmuir 26(8), 2010
PMID: 20014785

Weber, 2007
Dynamics in the plasma membrane: how to combine fluidity and order.
Marguet D, Lenne PF, Rigneault H, He HT., EMBO J. 25(15), 2006
PMID: 16900097

Yi, J. Phys.: Conf. Ser. 251(), 2010
Effect of pH and ibuprofen on the phospholipid bilayer bending modulus.
Boggara MB, Faraone A, Krishnamoorti R., J Phys Chem B 114(24), 2010
PMID: 20518571
Bending stiffness of biological membranes: what can be measured by neutron spin echo?
Mell M, Moleiro LH, Hertle Y, Fouquet P, Schweins R, Lopez-Montero I, Hellweg T, Monroy F., Eur Phys J E Soft Matter 36(7), 2013
PMID: 23852577
Bilayer undulation dynamics in unilamellar phospholipid vesicles: effect of temperature, cholesterol and trehalose.
Bruning BA, Prevost S, Stehle R, Steitz R, Falus P, Farago B, Hellweg T., Biochim. Biophys. Acta 1838(10), 2014
PMID: 24950248
Bending elasticities of model membranes: influences of temperature and sterol content.
Meleard P, Gerbeaud C, Pott T, Fernandez-Puente L, Bivas I, Mitov MD, Dufourcq J, Bothorel P., Biophys. J. 72(6), 1997
PMID: 9168037

Du, Electrochim. Acta 51(), 2006

Yi, Biophys. J. 160(), 2012

Yi, J. Phys.: Condens. Matter 21(), 2009
Elastic curvature constants of lipid monolayers and bilayers.
Marsh D., Chem. Phys. Lipids 144(2), 2006
PMID: 17045578
Nanosecond lipid dynamics in membranes containing cholesterol.
Armstrong CL, Haussler W, Seydel T, Katsaras J, Rheinstadter MC., Soft Matter 10(15), 2014
PMID: 24647350
Fluctuation dynamics of bilayer vesicles with intermonolayer sliding: experiment and theory.
Mell M, Moleiro LH, Hertle Y, Lopez-Montero I, Cao FJ, Fouquet P, Hellweg T, Monroy F., Chem. Phys. Lipids 185(), 2014
PMID: 25455136
All-optical measurements of the bending rigidity of lipid-vesicle membranes across structural phase transitions.
Lee CH, Lin WC, Wang J., Phys Rev E Stat Nonlin Soft Matter Phys 64(2 Pt 1), 2001
PMID: 11497555

Marquardt, J. Soc. Ind. Appl. Math. 11(), 1963

Levenberg, Q. Appl. Math. 2(), 1944

Dellnitz, J. Optim. Theory Appl. 124(), 2005
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