Differential transcriptomic analysis reveals hidden light response in Streptomyces lividans

Koepff J, Morschett H, Busche T, Winkler A, Kalinowski J, Wiechert W, Oldiges M (2018)
BIOTECHNOLOGY PROGRESS 34(1): 287-292.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Recently, a comprehensive screening workflow for the filamentous bacterium Streptomyces lividans, a highly performant source for pharmaceutically active agents was introduced. This framework used parallelized cultivation in microtiter plates to efficiently accelerate early upstream process development. Focusing on growth performance, cultivation was successfully scaled-up to 1 L stirred tank reactors. However, metabolic adaptation was observed on the transcriptomic level as among others, several genes incorporated in light response were upregulated during bioreactor cultivation. Despite it was assumed that this was attributed to the fact that reactor cultivations were performed in glass vessels exposed to daylight and artificial room light, this setup did not allow distinguishing exclusively between light and other effects. Upon that, the present study directly investigates the influence of light by defined illumination of microtiter plate cultures. Almost identical growth performance was observed for cultures grown in the dark or with illumination. Transcriptomics revealed the upregulation of seven genes of which 6 have previously been described to be relevant for carotenoid synthesis and its regulation. These pigments are effective quenchers of reactive oxygen species. The seventh transcript coded for a photo-lyase incorporated in UV-damage repair of DNA further confirming induced light response. However, this was fully compensated by metabolic adaptation on the transcriptomic level and overall process performance was maintained. Consequently, environmental conditions need extremely careful control and evaluation during in-depth omics analysis of bioprocesses. Otherwise metabolic adaptation induced by such issues can easily be misinterpreted, especially during studies addressing cultivation system comparisons. (c) 2017 American Institute of Chemical Engineers Biotechnol. Prog., 34:287-292, 2018
Stichworte
Streptomyces lividans; miniaturized cultivation; parallelized; cultivation; light response; transcriptomic analysis; high-throughput; bioprocess development; Photo BioLector
Erscheinungsjahr
2018
Zeitschriftentitel
BIOTECHNOLOGY PROGRESS
Band
34
Ausgabe
1
Seite(n)
287-292
ISSN
8756-7938
eISSN
1520-6033
Page URI
https://pub.uni-bielefeld.de/record/2918672

Zitieren

Koepff J, Morschett H, Busche T, et al. Differential transcriptomic analysis reveals hidden light response in Streptomyces lividans. BIOTECHNOLOGY PROGRESS. 2018;34(1):287-292.
Koepff, J., Morschett, H., Busche, T., Winkler, A., Kalinowski, J., Wiechert, W., & Oldiges, M. (2018). Differential transcriptomic analysis reveals hidden light response in Streptomyces lividans. BIOTECHNOLOGY PROGRESS, 34(1), 287-292. doi:10.1002/btpr.2566
Koepff, J., Morschett, H., Busche, T., Winkler, A., Kalinowski, J., Wiechert, W., and Oldiges, M. (2018). Differential transcriptomic analysis reveals hidden light response in Streptomyces lividans. BIOTECHNOLOGY PROGRESS 34, 287-292.
Koepff, J., et al., 2018. Differential transcriptomic analysis reveals hidden light response in Streptomyces lividans. BIOTECHNOLOGY PROGRESS, 34(1), p 287-292.
J. Koepff, et al., “Differential transcriptomic analysis reveals hidden light response in Streptomyces lividans”, BIOTECHNOLOGY PROGRESS, vol. 34, 2018, pp. 287-292.
Koepff, J., Morschett, H., Busche, T., Winkler, A., Kalinowski, J., Wiechert, W., Oldiges, M.: Differential transcriptomic analysis reveals hidden light response in Streptomyces lividans. BIOTECHNOLOGY PROGRESS. 34, 287-292 (2018).
Koepff, Joachim, Morschett, Holger, Busche, Tobias, Winkler, Anika, Kalinowski, Jörn, Wiechert, Wolfgang, and Oldiges, Marco. “Differential transcriptomic analysis reveals hidden light response in Streptomyces lividans”. BIOTECHNOLOGY PROGRESS 34.1 (2018): 287-292.

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