Single-bacterial genomics validates rich and varied specialized metabolism of uncultivated Entotheonella sponge symbionts

Mori T, Cahn JKB, Wilson MC, Meoded RA, Wiebach V, Martinez AFC, Helfrich EJN, Albersmeier A, Wibberg D, Datwyler S, Keren R, et al. (2018)
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 115(8): 1718-1723.

Zeitschriftenaufsatz | Veröffentlicht| Englisch
 
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Autor*in
Mori, Tetsushi; Cahn, Jackson K. B.; Wilson, Micheal C.; Meoded, Roy A.; Wiebach, Vincent; Martinez, Ana Flavia Canovas; Helfrich, Eric J. N.; Albersmeier, AndreasUniBi; Wibberg, DanielUniBi; Datwyler, Steven; Keren, Ray; Lavy, Adi
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Abstract / Bemerkung
Marine sponges are prolific sources of unique bioactive natural products. The sponge Theonella swinhoei is represented by several distinct variants with largely nonoverlapping chemistry. For the Japanese chemotype Y harboring diverse complex polyketides and peptides, we previously provided genomic and functional evidence that a single symbiont, the filamentous, multicellular organism "Candidatus Entotheonella factor,"produces almost all of these compounds. To obtain further insights into the chemistry of "Entotheonella," we investigated another phylotype, "Candidatus Entotheonella serta," present in the T. swinhoei WA sponge chemotype, a source of theonellamide- and misakinolide-type compounds. Unexpectedly, considering the lower chemical diversity, sequencing of individual bacterial filaments revealed an even larger number of biosynthetic gene regions than for Ca. E. factor, with virtually no overlap. These included genes for misakinolide and theonellamide biosynthesis, the latter assigned by comparative genomic and metabolic analysis of a T. swinhoei chemotype from Israel, and by biochemical studies. The data suggest that both compound families, which were among the earliest model substances to study bacterial producers in sponges, originate from the same bacterium in T. swinhoei WA. They also add evidence that metabolic richness and variability could be a more general feature of Entotheonella symbionts.
Stichworte
uncultivated bacteria; sponges; single-cell genomics
Erscheinungsjahr
2018
Zeitschriftentitel
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Band
115
Ausgabe
8
Seite(n)
1718-1723
ISSN
0027-8424
Page URI
https://pub.uni-bielefeld.de/record/2918662

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Mori T, Cahn JKB, Wilson MC, et al. Single-bacterial genomics validates rich and varied specialized metabolism of uncultivated Entotheonella sponge symbionts. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2018;115(8):1718-1723.
Mori, T., Cahn, J. K. B., Wilson, M. C., Meoded, R. A., Wiebach, V., Martinez, A. F. C., Helfrich, E. J. N., et al. (2018). Single-bacterial genomics validates rich and varied specialized metabolism of uncultivated Entotheonella sponge symbionts. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 115(8), 1718-1723. doi:10.1073/pnas.1715496115
Mori, T., Cahn, J. K. B., Wilson, M. C., Meoded, R. A., Wiebach, V., Martinez, A. F. C., Helfrich, E. J. N., Albersmeier, A., Wibberg, D., Datwyler, S., et al. (2018). Single-bacterial genomics validates rich and varied specialized metabolism of uncultivated Entotheonella sponge symbionts. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 115, 1718-1723.
Mori, T., et al., 2018. Single-bacterial genomics validates rich and varied specialized metabolism of uncultivated Entotheonella sponge symbionts. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 115(8), p 1718-1723.
T. Mori, et al., “Single-bacterial genomics validates rich and varied specialized metabolism of uncultivated Entotheonella sponge symbionts”, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 115, 2018, pp. 1718-1723.
Mori, T., Cahn, J.K.B., Wilson, M.C., Meoded, R.A., Wiebach, V., Martinez, A.F.C., Helfrich, E.J.N., Albersmeier, A., Wibberg, D., Datwyler, S., Keren, R., Lavy, A., Rückert, C., Ilan, M., Kalinowski, J., Matsunaga, S., Takeyama, H., Piel, J.: Single-bacterial genomics validates rich and varied specialized metabolism of uncultivated Entotheonella sponge symbionts. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 115, 1718-1723 (2018).
Mori, Tetsushi, Cahn, Jackson K. B., Wilson, Micheal C., Meoded, Roy A., Wiebach, Vincent, Martinez, Ana Flavia Canovas, Helfrich, Eric J. N., Albersmeier, Andreas, Wibberg, Daniel, Datwyler, Steven, Keren, Ray, Lavy, Adi, Rückert, Christian, Ilan, Micha, Kalinowski, Jörn, Matsunaga, Shigeki, Takeyama, Haruko, and Piel, Joern. “Single-bacterial genomics validates rich and varied specialized metabolism of uncultivated Entotheonella sponge symbionts”. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 115.8 (2018): 1718-1723.

2 Zitationen in Europe PMC

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