Metabolite profiling at the cellular and subcellular level reveals metabolites associated with salinity tolerance in sugar beet

Hossain MS, Persicke M, ElSayed AI, Kalinowski J, Dietz K-J (2017)
Journal of Experimental Botany 68(21-22): 5961-5976.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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Abstract / Bemerkung
Sugar beet is among the most salt-tolerant crops. This study aimed to investigate the metabolic adaptation of sugar beet to salt stress at the cellular and subcellular levels. Seedlings were grown hydroponically and subjected to stepwise increases in salt stress up to 300 mM NaCl. Highly enriched fractions of chloroplasts were obtained by nonaqueous fractionation using organic solvents. Total leaf metabolites and metabolites in chloroplasts were profiled at 3 h and 14 d after reaching the maximum salinity stress of 300 mM NaCl. Metabolite profiling by gas chromatography- mass spectrometry (GC-MS) resulted in the identification of a total of 83 metabolites in leaves and chloroplasts under control and stress conditions. There was a lower abundance of Calvin cycle metabolites under salinity whereas there was a higher abundance of oxidative pentose phosphate cycle metabolites such as 6-phosphogluconate. Accumulation of ribose-5-phosphate and ribulose-5-phosphate coincided with limitation of carbon fixation by ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). Increases in glycolate and serine levels indicated that photorespiratory metabolism was stimulated in salt-stressed sugar beet. Compatible solutes such as proline, mannitol, and putrescine accumulated mostly outside the chloroplasts. Within the chloroplast, putrescine had the highest relative level and probably assisted in the acclimation of sugar beet to high salinity stress. The results provide new information on the contribution of chloroplasts and the extra-chloroplast space to salinity tolerance via metabolic adjustment in sugar beet.
Stichworte
Chloroplast; metabolite profiling; non-aqueous fractionation; photosynthesis; salinity stress; sugar beet
Erscheinungsjahr
2017
Zeitschriftentitel
Journal of Experimental Botany
Band
68
Ausgabe
21-22
Seite(n)
5961-5976
ISSN
0022-0957
eISSN
1460-2431
Page URI
https://pub.uni-bielefeld.de/record/2916403

Zitieren

Hossain MS, Persicke M, ElSayed AI, Kalinowski J, Dietz K-J. Metabolite profiling at the cellular and subcellular level reveals metabolites associated with salinity tolerance in sugar beet. Journal of Experimental Botany. 2017;68(21-22):5961-5976.
Hossain, M. S., Persicke, M., ElSayed, A. I., Kalinowski, J., & Dietz, K. - J. (2017). Metabolite profiling at the cellular and subcellular level reveals metabolites associated with salinity tolerance in sugar beet. Journal of Experimental Botany, 68(21-22), 5961-5976. doi:10.1093/jxb/erx388
Hossain, M. S., Persicke, M., ElSayed, A. I., Kalinowski, J., and Dietz, K. - J. (2017). Metabolite profiling at the cellular and subcellular level reveals metabolites associated with salinity tolerance in sugar beet. Journal of Experimental Botany 68, 5961-5976.
Hossain, M.S., et al., 2017. Metabolite profiling at the cellular and subcellular level reveals metabolites associated with salinity tolerance in sugar beet. Journal of Experimental Botany, 68(21-22), p 5961-5976.
M.S. Hossain, et al., “Metabolite profiling at the cellular and subcellular level reveals metabolites associated with salinity tolerance in sugar beet”, Journal of Experimental Botany, vol. 68, 2017, pp. 5961-5976.
Hossain, M.S., Persicke, M., ElSayed, A.I., Kalinowski, J., Dietz, K.-J.: Metabolite profiling at the cellular and subcellular level reveals metabolites associated with salinity tolerance in sugar beet. Journal of Experimental Botany. 68, 5961-5976 (2017).
Hossain, M. Sazzad, Persicke, Marcus, ElSayed, Abdelaleim Ismail, Kalinowski, Jörn, and Dietz, Karl-Josef. “Metabolite profiling at the cellular and subcellular level reveals metabolites associated with salinity tolerance in sugar beet”. Journal of Experimental Botany 68.21-22 (2017): 5961-5976.

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