The MalR type regulator AcrC is a transcriptional repressor of acarbose biosynthetic genes in Actinoplanes sp. SE50/110
Wolf T, Droste J, Gren T, Ortseifen V, Schneiker-Bekel S, Zemke T, Pühler A, Kalinowski J (2017)
BMC Genomics 18(1): 562.
Zeitschriftenaufsatz
| Veröffentlicht | Englisch
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Einrichtung
Abstract / Bemerkung
Background
Acarbose is used in the treatment of diabetes mellitus type II and is produced by Actinoplanes sp. SE50/110. Although the biosynthesis of acarbose has been intensively studied, profound knowledge about transcription factors involved in acarbose biosynthesis and their binding sites has been missing until now. In contrast to acarbose biosynthetic gene clusters in Streptomyces spp., the corresponding gene cluster of Actinoplanes sp. SE50/110 lacks genes for transcriptional regulators.
Results
The acarbose regulator C (AcrC) was identified through an in silico approach by aligning the LacI family regulators of acarbose biosynthetic gene clusters in Streptomyces spp. with the Actinoplanes sp. SE50/110 genome. The gene for acrC, located in a head-to-head arrangement with the maltose/maltodextrin ABC transporter malEFG operon, was deleted by introducing PCR targeting for Actinoplanes sp. SE50/110. Characterization was carried out through cultivation experiments, genome-wide microarray hybridizations, and RT-qPCR as well as electrophoretic mobility shift assays for the elucidation of binding motifs. The results show that AcrC binds to the intergenic region between acbE and acbD in Actinoplanes sp. SE50/110 and acts as a transcriptional repressor on these genes. The transcriptomic profile of the wild type was reconstituted through a complementation of the deleted acrC gene. Additionally, regulatory sequence motifs for the binding of AcrC were identified in the intergenic region of acbE and acbD. It was shown that AcrC expression influences acarbose formation in the early growth phase. Interestingly, AcrC does not regulate the malEFG operon.
Conclusions
This study characterizes the first known transcription factor of the acarbose biosynthetic gene cluster in Actinoplanes sp. SE50/110. It therefore represents an important step for understanding the regulatory network of this organism. Based on this work, rational strain design for improving the biotechnological production of acarbose can now be implemented.
Stichworte
Actinoplanes;
Acarbose;
MalR;
AcrC;
Transcriptional regulation;
Actinomycetes
Erscheinungsjahr
2017
Zeitschriftentitel
BMC Genomics
Band
18
Ausgabe
1
Art.-Nr.
562
ISSN
1471-2164
eISSN
1471-2164
Finanzierungs-Informationen
Open-Access-Publikationskosten wurden durch die Deutsche Forschungsgemeinschaft und die Universität Bielefeld gefördert.
Page URI
https://pub.uni-bielefeld.de/record/2913116
Zitieren
Wolf T, Droste J, Gren T, et al. The MalR type regulator AcrC is a transcriptional repressor of acarbose biosynthetic genes in Actinoplanes sp. SE50/110. BMC Genomics. 2017;18(1): 562.
Wolf, T., Droste, J., Gren, T., Ortseifen, V., Schneiker-Bekel, S., Zemke, T., Pühler, A., et al. (2017). The MalR type regulator AcrC is a transcriptional repressor of acarbose biosynthetic genes in Actinoplanes sp. SE50/110. BMC Genomics, 18(1), 562. https://doi.org/10.1186/s12864-017-3941-x
Wolf, Timo, Droste, Julian, Gren, Tetiana, Ortseifen, Vera, Schneiker-Bekel, Susanne, Zemke, Till, Pühler, Alfred, and Kalinowski, Jörn. 2017. “The MalR type regulator AcrC is a transcriptional repressor of acarbose biosynthetic genes in Actinoplanes sp. SE50/110”. BMC Genomics 18 (1): 562.
Wolf, T., Droste, J., Gren, T., Ortseifen, V., Schneiker-Bekel, S., Zemke, T., Pühler, A., and Kalinowski, J. (2017). The MalR type regulator AcrC is a transcriptional repressor of acarbose biosynthetic genes in Actinoplanes sp. SE50/110. BMC Genomics 18:562.
Wolf, T., et al., 2017. The MalR type regulator AcrC is a transcriptional repressor of acarbose biosynthetic genes in Actinoplanes sp. SE50/110. BMC Genomics, 18(1): 562.
T. Wolf, et al., “The MalR type regulator AcrC is a transcriptional repressor of acarbose biosynthetic genes in Actinoplanes sp. SE50/110”, BMC Genomics, vol. 18, 2017, : 562.
Wolf, T., Droste, J., Gren, T., Ortseifen, V., Schneiker-Bekel, S., Zemke, T., Pühler, A., Kalinowski, J.: The MalR type regulator AcrC is a transcriptional repressor of acarbose biosynthetic genes in Actinoplanes sp. SE50/110. BMC Genomics. 18, : 562 (2017).
Wolf, Timo, Droste, Julian, Gren, Tetiana, Ortseifen, Vera, Schneiker-Bekel, Susanne, Zemke, Till, Pühler, Alfred, and Kalinowski, Jörn. “The MalR type regulator AcrC is a transcriptional repressor of acarbose biosynthetic genes in Actinoplanes sp. SE50/110”. BMC Genomics 18.1 (2017): 562.
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2 Zitationen in Europe PMC
Daten bereitgestellt von Europe PubMed Central.
Evaluation of vector systems and promoters for overexpression of the acarbose biosynthesis gene acbC in Actinoplanes sp. SE50/110.
Schaffert L, März C, Burkhardt L, Droste J, Brandt D, Busche T, Rosen W, Schneiker-Bekel S, Persicke M, Pühler A, Kalinowski J., Microb Cell Fact 18(1), 2019
PMID: 31253141
Schaffert L, März C, Burkhardt L, Droste J, Brandt D, Busche T, Rosen W, Schneiker-Bekel S, Persicke M, Pühler A, Kalinowski J., Microb Cell Fact 18(1), 2019
PMID: 31253141
Regulation of antibiotic production in Actinobacteria: new perspectives from the post-genomic era.
van der Heul HU, Bilyk BL, McDowall KJ, Seipke RF, van Wezel GP., Nat Prod Rep 35(6), 2018
PMID: 29721572
van der Heul HU, Bilyk BL, McDowall KJ, Seipke RF, van Wezel GP., Nat Prod Rep 35(6), 2018
PMID: 29721572
63 References
Daten bereitgestellt von Europe PubMed Central.
Creutzfeldt W., 1988
Significance of testing platelet functions in vitro.
Holmsen H., Eur. J. Clin. Invest. 24 Suppl 1(), 1994
PMID: 8013528
Holmsen H., Eur. J. Clin. Invest. 24 Suppl 1(), 1994
PMID: 8013528
Biotechnology and molecular biology of the alpha-glucosidase inhibitor acarbose.
Wehmeier UF, Piepersberg W., Appl. Microbiol. Biotechnol. 63(6), 2003
PMID: 14669056
Wehmeier UF, Piepersberg W., Appl. Microbiol. Biotechnol. 63(6), 2003
PMID: 14669056
The biosynthesis and metabolism of Acarbose in Actinoplanes sp. SE 50/110: a progress report
Wehmeier UF., 2003
Wehmeier UF., 2003
The complete genome sequence of the acarbose producer Actinoplanes sp. SE50/110.
Schwientek P, Szczepanowski R, Ruckert C, Kalinowski J, Klein A, Selber K, Wehmeier UF, Stoye J, Puhler A., BMC Genomics 13(), 2012
PMID: 22443545
Schwientek P, Szczepanowski R, Ruckert C, Kalinowski J, Klein A, Selber K, Wehmeier UF, Stoye J, Puhler A., BMC Genomics 13(), 2012
PMID: 22443545
Chemistry and biochemistry of microbial α-Glucosidase inhibitors
Truscheit E, Frommer W, Junge B, Müller L, Schmidt DD, Wingender W., 1981
Truscheit E, Frommer W, Junge B, Müller L, Schmidt DD, Wingender W., 1981
Members of the genus Actinoplanes and their antibiotics.
Parenti F, Coronelli C., Annu. Rev. Microbiol. 33(), 1979
PMID: 386928
Parenti F, Coronelli C., Annu. Rev. Microbiol. 33(), 1979
PMID: 386928
Actinoplanes
Vobis G, Schäfer J, Kämpfer P., 2012
Vobis G, Schäfer J, Kämpfer P., 2012
Biosynthesis of the C(7)-cyclitol moiety of acarbose in Actinoplanes species SE50/110. 7-O-phosphorylation of the initial cyclitol precursor leads to proposal of a new biosynthetic pathway.
Zhang CS, Stratmann A, Block O, Bruckner R, Podeschwa M, Altenbach HJ, Wehmeier UF, Piepersberg W., J. Biol. Chem. 277(25), 2002
PMID: 11937512
Zhang CS, Stratmann A, Block O, Bruckner R, Podeschwa M, Altenbach HJ, Wehmeier UF, Piepersberg W., J. Biol. Chem. 277(25), 2002
PMID: 11937512
The cytosolic and extracellular proteomes of Actinoplanes sp. SE50/110 led to the identification of gene products involved in acarbose metabolism.
Wendler S, Hurtgen D, Kalinowski J, Klein A, Niehaus K, Schulte F, Schwientek P, Wehlmann H, Wehmeier UF, Puhler A., J. Biotechnol. 167(2), 2012
PMID: 22944206
Wendler S, Hurtgen D, Kalinowski J, Klein A, Niehaus K, Schulte F, Schwientek P, Wehlmann H, Wehmeier UF, Puhler A., J. Biotechnol. 167(2), 2012
PMID: 22944206
Comparative proteome analysis of Actinoplanes sp. SE50/110 grown with maltose or glucose shows minor differences for acarbose biosynthesis proteins but major differences for saccharide transporters.
Wendler S, Otto A, Ortseifen V, Bonn F, Neshat A, Schneiker-Bekel S, Wolf T, Zemke T, Wehmeier UF, Hecker M, Kalinowski J, Becher D, Puhler A., J Proteomics 131(), 2015
PMID: 26597626
Wendler S, Otto A, Ortseifen V, Bonn F, Neshat A, Schneiker-Bekel S, Wolf T, Zemke T, Wehmeier UF, Hecker M, Kalinowski J, Becher D, Puhler A., J Proteomics 131(), 2015
PMID: 26597626
Genetic engineering in Actinoplanes sp. SE50/110 - development of an intergeneric conjugation system for the introduction of actinophage-based integrative vectors.
Gren T, Ortseifen V, Wibberg D, Schneiker-Bekel S, Bednarz H, Niehaus K, Zemke T, Persicke M, Puhler A, Kalinowski J., J. Biotechnol. 232(), 2016
PMID: 27181842
Gren T, Ortseifen V, Wibberg D, Schneiker-Bekel S, Bednarz H, Niehaus K, Zemke T, Persicke M, Puhler A, Kalinowski J., J. Biotechnol. 232(), 2016
PMID: 27181842
Targeted genome editing in the rare actinomycete Actinoplanes sp. SE50/110 by using the CRISPR/Cas9 System.
Wolf T, Gren T, Thieme E, Wibberg D, Zemke T, Puhler A, Kalinowski J., J. Biotechnol. 231(), 2016
PMID: 27262504
Wolf T, Gren T, Thieme E, Wibberg D, Zemke T, Puhler A, Kalinowski J., J. Biotechnol. 231(), 2016
PMID: 27262504
Genome improvement of the acarbose producer Actinoplanes sp. SE50/110 and annotation refinement based on RNA-seq analysis.
Wolf T, Schneiker-Bekel S, Neshat A, Ortseifen V, Wibberg D, Zemke T, Puhler A, Kalinowski J., J. Biotechnol. 251(), 2017
PMID: 28427920
Wolf T, Schneiker-Bekel S, Neshat A, Ortseifen V, Wibberg D, Zemke T, Puhler A, Kalinowski J., J. Biotechnol. 251(), 2017
PMID: 28427920
The gac-gene cluster for the production of acarbose from Streptomyces glaucescens GLA.O: identification, isolation and characterization.
Rockser Y, Wehmeier UF., J. Biotechnol. 140(1-2), 2008
PMID: 19059289
Rockser Y, Wehmeier UF., J. Biotechnol. 140(1-2), 2008
PMID: 19059289
Draft genome sequence of Streptomyces coelicoflavus ZG0656 reveals the putative biosynthetic gene cluster of acarviostatin family α-amylase inhibitors.
Guo X, Geng P, Bai F, Bai G, Sun T, Li X, Shi L, Zhong Q., Lett. Appl. Microbiol. 55(2), 2012
PMID: 22691180
Guo X, Geng P, Bai F, Bai G, Sun T, Li X, Shi L, Zhong Q., Lett. Appl. Microbiol. 55(2), 2012
PMID: 22691180
Cloning, characterisation and regulation of an alpha-amylase gene from Streptomyces venezuelae.
Virolle MJ, Long CM, Chang S, Bibb MJ., Gene 74(2), 1988
PMID: 3266752
Virolle MJ, Long CM, Chang S, Bibb MJ., Gene 74(2), 1988
PMID: 3266752
Sequences involved in growth-phase-dependent expression and glucose repression of a Streptomyces alpha-amylase gene.
Virolle MJ, Gagnat J., Microbiology (Reading, Engl.) 140 ( Pt 5)(), 1994
PMID: 8025672
Virolle MJ, Gagnat J., Microbiology (Reading, Engl.) 140 ( Pt 5)(), 1994
PMID: 8025672
Complete genome sequence of the actinobacterium Streptomyces glaucescens GLA.O (DSM 40922) consisting of a linear chromosome and one linear plasmid.
Ortseifen V, Winkler A, Albersmeier A, Wendler S, Puhler A, Kalinowski J, Ruckert C., J. Biotechnol. 194(), 2014
PMID: 25499805
Ortseifen V, Winkler A, Albersmeier A, Wendler S, Puhler A, Kalinowski J, Ruckert C., J. Biotechnol. 194(), 2014
PMID: 25499805
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ., Nucleic Acids Res. 25(17), 1997
PMID: 9254694
Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ., Nucleic Acids Res. 25(17), 1997
PMID: 9254694
MUSCLE: multiple sequence alignment with high accuracy and high throughput.
Edgar RC., Nucleic Acids Res. 32(5), 2004
PMID: 15034147
Edgar RC., Nucleic Acids Res. 32(5), 2004
PMID: 15034147
Substrate induction and glucose repression of maltose utilization by Streptomyces coelicolor A3(2) is controlled by malR, a member of the lacl-galR family of regulatory genes.
van Wezel GP, White J, Young P, Postma PW, Bibb MJ., Mol. Microbiol. 23(3), 1997
PMID: 9044287
van Wezel GP, White J, Young P, Postma PW, Bibb MJ., Mol. Microbiol. 23(3), 1997
PMID: 9044287
Synthesis of the Streptomyces lividans maltodextrin ABC transporter depends on the presence of the regulator MalR.
Schlosser A, Weber A, Schrempf H., FEMS Microbiol. Lett. 196(1), 2001
PMID: 11257552
Schlosser A, Weber A, Schrempf H., FEMS Microbiol. Lett. 196(1), 2001
PMID: 11257552
PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin.
Gust B, Challis GL, Fowler K, Kieser T, Chater KF., Proc. Natl. Acad. Sci. U.S.A. 100(4), 2003
PMID: 12563033
Gust B, Challis GL, Fowler K, Kieser T, Chater KF., Proc. Natl. Acad. Sci. U.S.A. 100(4), 2003
PMID: 12563033
AUTHOR UNKNOWN, 0
Carbon source dependent biosynthesis of acarviose metabolites in Actinoplanes sp. SE50/110.
Wendler S, Ortseifen V, Persicke M, Klein A, Neshat A, Niehaus K, Schneiker-Bekel S, Walter F, Wehmeier UF, Kalinowski J, Puhler A., J. Biotechnol. 191(), 2014
PMID: 25169663
Wendler S, Ortseifen V, Persicke M, Klein A, Neshat A, Niehaus K, Schneiker-Bekel S, Walter F, Wehmeier UF, Kalinowski J, Puhler A., J. Biotechnol. 191(), 2014
PMID: 25169663
Comprehensive proteome analysis of Actinoplanes sp. SE50/110 highlighting the location of proteins encoded by the acarbose and the pyochelin biosynthesis gene cluster.
Wendler S, Otto A, Ortseifen V, Bonn F, Neshat A, Schneiker-Bekel S, Walter F, Wolf T, Zemke T, Wehmeier UF, Hecker M, Kalinowski J, Becher D, Puhler A., J Proteomics 125(), 2015
PMID: 25896738
Wendler S, Otto A, Ortseifen V, Bonn F, Neshat A, Schneiker-Bekel S, Walter F, Wolf T, Zemke T, Wehmeier UF, Hecker M, Kalinowski J, Becher D, Puhler A., J Proteomics 125(), 2015
PMID: 25896738
Towards an understanding of protein-DNA recognition.
Rhodes D, Schwabe JW, Chapman L, Fairall L., Philos. Trans. R. Soc. Lond., B, Biol. Sci. 351(1339), 1996
PMID: 8735272
Rhodes D, Schwabe JW, Chapman L, Fairall L., Philos. Trans. R. Soc. Lond., B, Biol. Sci. 351(1339), 1996
PMID: 8735272
Prokaryotic transcription regulators: more than just the helix-turn-helix motif.
Huffman JL, Brennan RG., Curr. Opin. Struct. Biol. 12(1), 2002
PMID: 11839496
Huffman JL, Brennan RG., Curr. Opin. Struct. Biol. 12(1), 2002
PMID: 11839496
A family of bacterial regulators homologous to Gal and Lac repressors.
Weickert MJ, Adhya S., J. Biol. Chem. 267(22), 1992
PMID: 1639817
Weickert MJ, Adhya S., J. Biol. Chem. 267(22), 1992
PMID: 1639817
Allostery in the LacI/GalR family: variations on a theme.
Swint-Kruse L, Matthews KS., Curr. Opin. Microbiol. 12(2), 2009
PMID: 19269243
Swint-Kruse L, Matthews KS., Curr. Opin. Microbiol. 12(2), 2009
PMID: 19269243
Site-specific recombination strategies for engineering actinomycete genomes.
Herrmann S, Siegl T, Luzhetska M, Petzke L, Jilg C, Welle E, Erb A, Leadlay PF, Bechthold A, Luzhetskyy A., Appl. Environ. Microbiol. 78(6), 2012
PMID: 22247163
Herrmann S, Siegl T, Luzhetska M, Petzke L, Jilg C, Welle E, Erb A, Leadlay PF, Bechthold A, Luzhetskyy A., Appl. Environ. Microbiol. 78(6), 2012
PMID: 22247163
Genome engineering in actinomycetes using site-specific recombinases.
Myronovskyi M, Luzhetskyy A., Appl. Microbiol. Biotechnol. 97(11), 2013
PMID: 23584280
Myronovskyi M, Luzhetskyy A., Appl. Microbiol. Biotechnol. 97(11), 2013
PMID: 23584280
Marker removal from actinomycetes genome using Flp recombinase.
Fedoryshyn M, Petzke L, Welle E, Bechthold A, Luzhetskyy A., Gene 419(1-2), 2008
PMID: 18550297
Fedoryshyn M, Petzke L, Welle E, Bechthold A, Luzhetskyy A., Gene 419(1-2), 2008
PMID: 18550297
Use of the native flp gene to generate in-frame unmarked mutations in Streptomyces spp.
Zelyas N, Tahlan K, Jensen SE., Gene 443(1-2), 2009
PMID: 19446014
Zelyas N, Tahlan K, Jensen SE., Gene 443(1-2), 2009
PMID: 19446014
Mapping and quantifying mammalian transcriptomes by RNA-Seq.
Mortazavi A, Williams BA, McCue K, Schaeffer L, Wold B., Nat. Methods 5(7), 2008
PMID: 18516045
Mortazavi A, Williams BA, McCue K, Schaeffer L, Wold B., Nat. Methods 5(7), 2008
PMID: 18516045
RNA-Seq: a revolutionary tool for transcriptomics.
Wang Z, Gerstein M, Snyder M., Nat. Rev. Genet. 10(1), 2009
PMID: 19015660
Wang Z, Gerstein M, Snyder M., Nat. Rev. Genet. 10(1), 2009
PMID: 19015660
The malEFG gene cluster of Streptomyces coelicolor A3(2): characterization, disruption and transcriptional analysis.
van Wezel GP, White J, Bibb MJ, Postma PW., Mol. Gen. Genet. 254(5), 1997
PMID: 9197422
van Wezel GP, White J, Bibb MJ, Postma PW., Mol. Gen. Genet. 254(5), 1997
PMID: 9197422
Amylase and chitinase genes in Streptomyces lividans are regulated by reg1, a pleiotropic regulatory gene.
Nguyen J, Francou F, Virolle MJ, Guerineau M., J. Bacteriol. 179(20), 1997
PMID: 9335287
Nguyen J, Francou F, Virolle MJ, Guerineau M., J. Bacteriol. 179(20), 1997
PMID: 9335287
The regulatory protein Reg1 of Streptomyces lividans binds the promoter region of several genes repressed by glucose
Nguyen J., 1999
Nguyen J., 1999
Cloning, characterization and regulation of an alpha-amylase gene from Streptomyces limosus.
Virolle MJ, Bibb MJ., Mol. Microbiol. 2(2), 1988
PMID: 3260002
Virolle MJ, Bibb MJ., Mol. Microbiol. 2(2), 1988
PMID: 3260002
Catabolite repression of alpha-amylase gene expression in Bacillus subtilis involves a trans-acting gene product homologous to the Escherichia coli lacl and galR repressors.
Henkin TM, Grundy FJ, Nicholson WL, Chambliss GH., Mol. Microbiol. 5(3), 1991
PMID: 1904524
Henkin TM, Grundy FJ, Nicholson WL, Chambliss GH., Mol. Microbiol. 5(3), 1991
PMID: 1904524
Maltose-Dependent Transcriptional Regulation of the mal Regulon by MalR in Streptococcus pneumoniae.
Afzal M, Shafeeq S, Manzoor I, Kuipers OP., PLoS ONE 10(6), 2015
PMID: 26030923
Afzal M, Shafeeq S, Manzoor I, Kuipers OP., PLoS ONE 10(6), 2015
PMID: 26030923
Chapter 19 Enzymology of Aminoglycoside Biosynthesis—Deduction from Gene Clusters
Wehmeier UF, Piepersberg W., 2009
Wehmeier UF, Piepersberg W., 2009
Identification, cloning, expression, and characterization of the extracellular acarbose-modifying glycosyltransferase, AcbD, from Actinoplanes sp. strain SE50.
Hemker M, Stratmann A, Goeke K, Schroder W, Lenz J, Piepersberg W, Pape H., J. Bacteriol. 183(15), 2001
PMID: 11443082
Hemker M, Stratmann A, Goeke K, Schroder W, Lenz J, Piepersberg W, Pape H., J. Bacteriol. 183(15), 2001
PMID: 11443082
Single amino acid mutations interchange the reaction specificities of cyclodextrin glycosyltransferase and the acarbose-modifying enzyme acarviosyl transferase.
Leemhuis H, Wehmeier UF, Dijkhuizen L., Biochemistry 43(41), 2004
PMID: 15476414
Leemhuis H, Wehmeier UF, Dijkhuizen L., Biochemistry 43(41), 2004
PMID: 15476414
Crystal structure of the lactose operon repressor and its complexes with DNA and inducer.
Lewis M, Chang G, Horton NC, Kercher MA, Pace HC, Schumacher MA, Brennan RG, Lu P., Science 271(5253), 1996
PMID: 8638105
Lewis M, Chang G, Horton NC, Kercher MA, Pace HC, Schumacher MA, Brennan RG, Lu P., Science 271(5253), 1996
PMID: 8638105
Lactose Repressor Protein: Functional Properties and Structure
Matthews KS, Nichols JC., 1998
Matthews KS, Nichols JC., 1998
The three operators of the lac operon cooperate in repression.
Oehler S, Eismann ER, Kramer H, Muller-Hill B., EMBO J. 9(4), 1990
PMID: 2182324
Oehler S, Eismann ER, Kramer H, Muller-Hill B., EMBO J. 9(4), 1990
PMID: 2182324
Interconvertible lac repressor-DNA loops revealed by single-molecule experiments.
Wong OK, Guthold M, Erie DA, Gelles J., PLoS Biol. 6(9), 2008
PMID: 18828671
Wong OK, Guthold M, Erie DA, Gelles J., PLoS Biol. 6(9), 2008
PMID: 18828671
Tetramer opening in LacI-mediated DNA looping.
Rutkauskas D, Zhan H, Matthews KS, Pavone FS, Vanzi F., Proc. Natl. Acad. Sci. U.S.A. 106(39), 2009
PMID: 19805348
Rutkauskas D, Zhan H, Matthews KS, Pavone FS, Vanzi F., Proc. Natl. Acad. Sci. U.S.A. 106(39), 2009
PMID: 19805348
Maltose/maltodextrin system of Escherichia coli: transport, metabolism, and regulation.
Boos W, Shuman H., Microbiol. Mol. Biol. Rev. 62(1), 1998
PMID: 9529892
Boos W, Shuman H., Microbiol. Mol. Biol. Rev. 62(1), 1998
PMID: 9529892
Roles of maltodextrin and glycogen phosphorylases in maltose utilization and glycogen metabolism in Corynebacterium glutamicum.
Seibold GM, Wurst M, Eikmanns BJ., Microbiology (Reading, Engl.) 155(Pt 2), 2009
PMID: 19202084
Seibold GM, Wurst M, Eikmanns BJ., Microbiology (Reading, Engl.) 155(Pt 2), 2009
PMID: 19202084
Characterization of maltose and maltotriose transport in the acarbose-producing bacterium Actinoplanes sp.
Brunkhorst C, Schneider E., Res. Microbiol. 156(8), 2005
PMID: 15939574
Brunkhorst C, Schneider E., Res. Microbiol. 156(8), 2005
PMID: 15939574
Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants.
Grant SG, Jessee J, Bloom FR, Hanahan D., Proc. Natl. Acad. Sci. U.S.A. 87(12), 1990
PMID: 2162051
Grant SG, Jessee J, Bloom FR, Hanahan D., Proc. Natl. Acad. Sci. U.S.A. 87(12), 1990
PMID: 2162051
Kieser T, Bibb MJ, Buttner MJ, Chater KF, Hopwood DA., 2000
Integrated DNA sequences in three streptomycetes form related autonomous plasmids after transfer to Streptomyces lividans.
Hopwood DA, Hintermann G, Kieser T, Wright HM., Plasmid 11(1), 1984
PMID: 6369354
Hopwood DA, Hintermann G, Kieser T, Wright HM., Plasmid 11(1), 1984
PMID: 6369354
Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp.
Bierman M, Logan R, O'Brien K, Seno ET, Rao RN, Schoner BE., Gene 116(1), 1992
PMID: 1628843
Bierman M, Logan R, O'Brien K, Seno ET, Rao RN, Schoner BE., Gene 116(1), 1992
PMID: 1628843
Enzymatic assembly of DNA molecules up to several hundred kilobases.
Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, Smith HO., Nat. Methods 6(5), 2009
PMID: 19363495
Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, Smith HO., Nat. Methods 6(5), 2009
PMID: 19363495
EMMA 2--a MAGE-compliant system for the collaborative analysis and integration of microarray data.
Dondrup M, Albaum SP, Griebel T, Henckel K, Junemann S, Kahlke T, Kleindt CK, Kuster H, Linke B, Mertens D, Mittard-Runte V, Neuweger H, Runte KJ, Tauch A, Tille F, Puhler A, Goesmann A., BMC Bioinformatics 10(), 2009
PMID: 19200358
Dondrup M, Albaum SP, Griebel T, Henckel K, Junemann S, Kahlke T, Kleindt CK, Kuster H, Linke B, Mertens D, Mittard-Runte V, Neuweger H, Runte KJ, Tauch A, Tille F, Puhler A, Goesmann A., BMC Bioinformatics 10(), 2009
PMID: 19200358
A vector system with temperature-sensitive replication for gene disruption and mutational cloning in streptomycetes
Muth G, Nußbaumer B, Wohlleben W, Pühler A., 1989
Muth G, Nußbaumer B, Wohlleben W, Pühler A., 1989
The Pfam protein families database: towards a more sustainable future.
Finn RD, Coggill P, Eberhardt RY, Eddy SR, Mistry J, Mitchell AL, Potter SC, Punta M, Qureshi M, Sangrador-Vegas A, Salazar GA, Tate J, Bateman A., Nucleic Acids Res. 44(D1), 2015
PMID: 26673716
Finn RD, Coggill P, Eberhardt RY, Eddy SR, Mistry J, Mitchell AL, Potter SC, Punta M, Qureshi M, Sangrador-Vegas A, Salazar GA, Tate J, Bateman A., Nucleic Acids Res. 44(D1), 2015
PMID: 26673716
Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data.
Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Meintjes P, Drummond A., Bioinformatics 28(12), 2012
PMID: 22543367
Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Meintjes P, Drummond A., Bioinformatics 28(12), 2012
PMID: 22543367
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Teil dieser Dissertation
Analysis of the transcriptional regulation of the acarbose biosynthesis gene cluster in Actinoplanes sp. SE50/110
Droste J (2021)
Bielefeld: Universität Bielefeld.
Droste J (2021)
Bielefeld: Universität Bielefeld.
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