Comprehensive proteome analysis of Actinoplanes sp SE50/110 highlighting the location of proteins encoded by the acarbose and the pyochelin biosynthesis gene cluster

Wendler S, Otto A, Ortseifen V, Bonn F, Neshat A, Schneiker-Bekel S, Walter F, Wolf T, Zemke T, Wehmeier UF, Hecker M, et al. (2015)
Journal of Proteomics 125: 1-16.

Zeitschriftenaufsatz | Veröffentlicht | Englisch
 
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DOI
Autor*in
Wendler, SergejUniBi; Otto, Andreas; Ortseifen, VeraUniBi ; Bonn, Florian; Neshat, ArminUniBi; Schneiker-Bekel, SusanneUniBi; Walter, FrederikUniBi; Wolf, TimoUniBi; Zemke, Till; Wehmeier, Udo F.; Hecker, Michael; Kalinowski, JörnUniBi
Alle
Einrichtung
Centrum für Biotechnologie > Arbeitsgruppe A. Pühler
Centrum für Biotechnologie > Graduate Center > Graduate Cluster Industrial Biotechnology
Fakultät für Biologie > Proteom- und Metabolomforschung
Abstract / Bemerkung
Acarbose is an alpha-glucosidase inhibitor produced by Actinoplanes sp. SE50/110 that is medically important due to its application in the treatment of type2 diabetes. In this work, a comprehensive proteome analysis of Actinoplanes sp. SE50/110 was carried out to determine the location of proteins of the acarbose (acb) and the putative pyochelin (pch) biosynthesis gene cluster. Therefore, a comprehensive state-of-the-art proteomics approach combining subcellular fractionation, shotgun proteomics and spectral counting to assess the relative abundance of proteins within fractions was applied. The analysis of four different proteome fractions (cytosolic, enriched membrane, membrane shaving and extracellular fraction) resulted in the identification of 1582 of the 8270 predicted proteins. All 22 Acb-proteins and 21 of the 23 Pch-proteins were detected. Predicted membrane-associated, integral membrane or extracellular proteins of the pch and the acb gene cluster were found among the most abundant proteins in corresponding fractions. Intracellular biosynthetic proteins of both gene clusters were not only detected in the cytosolic, but also in the enriched membrane fraction, indicating that the biosynthesis of acarbose and putative pyochelin metabolites takes place at the inner membrane. Biological significance Actinoplanes sp. SE50/110 is a natural producer of the alpha-glucosidase inhibitor acarbose, a bacterial secondary metabolite that is used as a drug for the treatment of type 2 diabetes, a disease which is a global pandemic that currently affects 387 million people and accounts for 11% of worldwide healthcare expenditures (www.idf.org). The work presented here is the first comprehensive investigation of protein localization and abundance in Actinoplanes sp. SE50/110 and provides an extensive source of information for the selection of genes for future mutational analysis and other hypothesis driven experiments. The conclusion that acarbose or pyochelin family siderophores are synthesized at the inner side of the cytoplasmic membrane determined from this work, indicates that studying corresponding intermediates will be challenging. In addition to previous studies on the genome and transcriptome, the work presented here demonstrates that the next omic level, the proteome, is now accessible for detailed physiological analysis of Actinoplanes sp. SE50/110, as well as mutants derived from this and related species. (C) 2015 Elsevier B.V. All rights reserved.
Stichworte
Actinoplanes; Comprehensive proteomics; Subcellular fractionation; Pyochelin; Membrane proteome acarbose
Erscheinungsjahr
2015
Zeitschriftentitel
Journal of Proteomics
Band
125
Seite(n)
1-16
ISSN
1874-3919
Page URI
https://pub.uni-bielefeld.de/record/2764307

Zitieren

Wendler S, Otto A, Ortseifen V, et al. Comprehensive proteome analysis of Actinoplanes sp SE50/110 highlighting the location of proteins encoded by the acarbose and the pyochelin biosynthesis gene cluster. Journal of Proteomics. 2015;125:1-16.
Wendler, S., Otto, A., Ortseifen, V., Bonn, F., Neshat, A., Schneiker-Bekel, S., Walter, F., et al. (2015). Comprehensive proteome analysis of Actinoplanes sp SE50/110 highlighting the location of proteins encoded by the acarbose and the pyochelin biosynthesis gene cluster. Journal of Proteomics, 125, 1-16. doi:10.1016/j.jprot.2015.04.013
Wendler, Sergej, Otto, Andreas, Ortseifen, Vera, Bonn, Florian, Neshat, Armin, Schneiker-Bekel, Susanne, Walter, Frederik, et al. 2015. “Comprehensive proteome analysis of Actinoplanes sp SE50/110 highlighting the location of proteins encoded by the acarbose and the pyochelin biosynthesis gene cluster”. Journal of Proteomics 125: 1-16.
Wendler, S., Otto, A., Ortseifen, V., Bonn, F., Neshat, A., Schneiker-Bekel, S., Walter, F., Wolf, T., Zemke, T., Wehmeier, U. F., et al. (2015). Comprehensive proteome analysis of Actinoplanes sp SE50/110 highlighting the location of proteins encoded by the acarbose and the pyochelin biosynthesis gene cluster. Journal of Proteomics 125, 1-16.
Wendler, S., et al., 2015. Comprehensive proteome analysis of Actinoplanes sp SE50/110 highlighting the location of proteins encoded by the acarbose and the pyochelin biosynthesis gene cluster. Journal of Proteomics, 125, p 1-16.
S. Wendler, et al., “Comprehensive proteome analysis of Actinoplanes sp SE50/110 highlighting the location of proteins encoded by the acarbose and the pyochelin biosynthesis gene cluster”, Journal of Proteomics, vol. 125, 2015, pp. 1-16.
Wendler, S., Otto, A., Ortseifen, V., Bonn, F., Neshat, A., Schneiker-Bekel, S., Walter, F., Wolf, T., Zemke, T., Wehmeier, U.F., Hecker, M., Kalinowski, J., Becher, D., Pühler, A.: Comprehensive proteome analysis of Actinoplanes sp SE50/110 highlighting the location of proteins encoded by the acarbose and the pyochelin biosynthesis gene cluster. Journal of Proteomics. 125, 1-16 (2015).
Wendler, Sergej, Otto, Andreas, Ortseifen, Vera, Bonn, Florian, Neshat, Armin, Schneiker-Bekel, Susanne, Walter, Frederik, Wolf, Timo, Zemke, Till, Wehmeier, Udo F., Hecker, Michael, Kalinowski, Jörn, Becher, Doerte, and Pühler, Alfred. “Comprehensive proteome analysis of Actinoplanes sp SE50/110 highlighting the location of proteins encoded by the acarbose and the pyochelin biosynthesis gene cluster”. Journal of Proteomics 125 (2015): 1-16.

5 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Evaluation of vector systems and promoters for overexpression of the acarbose biosynthesis gene acbC in Actinoplanes sp. SE50/110.
Schaffert L, März C, Burkhardt L, Droste J, Brandt D, Busche T, Rosen W, Schneiker-Bekel S, Persicke M, Pühler A, Kalinowski J., Microb Cell Fact 18(1), 2019
PMID: 31253141
Genome improvement of the acarbose producer Actinoplanes sp. SE50/110 and annotation refinement based on RNA-seq analysis.
Wolf T, Schneiker-Bekel S, Neshat A, Ortseifen V, Wibberg D, Zemke T, Pühler A, Kalinowski J., J Biotechnol 251(), 2017
PMID: 28427920
The MalR type regulator AcrC is a transcriptional repressor of acarbose biosynthetic genes in Actinoplanes sp. SE50/110.
Wolf T, Droste J, Gren T, Ortseifen V, Schneiker-Bekel S, Zemke T, Pühler A, Kalinowski J., BMC Genomics 18(1), 2017
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Comparative proteome analysis of Actinoplanes sp. SE50/110 grown with maltose or glucose shows minor differences for acarbose biosynthesis proteins but major differences for saccharide transporters.
Wendler S, Otto A, Ortseifen V, Bonn F, Neshat A, Schneiker-Bekel S, Wolf T, Zemke T, Wehmeier UF, Hecker M, Kalinowski J, Becher D, Pühler A., J Proteomics 131(), 2016
PMID: 26597626
Targeted genome editing in the rare actinomycete Actinoplanes sp. SE50/110 by using the CRISPR/Cas9 System.
Wolf T, Gren T, Thieme E, Wibberg D, Zemke T, Pühler A, Kalinowski J., J Biotechnol 231(), 2016
PMID: 27262504

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