Metabolic flux pattern of glucose utilization by Xanthomonas campestris pv. campestris: prevalent role of the Entner-Doudoroff pathway and minor fluxes through the pentose phosphate pathway and glycolysis

Schatschneider S, Huber C, Neuweger H, Watt T, Pühler A, Eisenreich W, Wittmann C, Niehaus K, Vorhölter F-J (2014)
Molecular BioSystems 10(10): 2663-2676.

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DOI
Autor*in
Schatschneider, SarahUniBi; Huber, Claudia; Neuweger, HeikoUniBi; Watt, TonyUniBi; Pühler, AlfredUniBi ; Eisenreich, Wolfgang; Wittmann, Christoph; Niehaus, KarstenUniBi; Vorhölter, Frank-JörgUniBi
Einrichtung
Centrum für Biotechnologie > Arbeitsgruppe A. Pühler
Centrum für Biotechnologie > Graduate Center > Graduate Cluster Industrial Biotechnology
Fakultät für Biologie > Proteom- und Metabolomforschung
Abstract / Bemerkung
The well-studied plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) synthesizes the biotechnologically important polysaccharide xanthan gum, which is also regarded as a virulence factor in plant interactions. In Xcc, sugars like glucose are utilized as a source to generate energy and biomass for growth and pathogenicity. In this study, we used [1-C-13]glucose as a tracer to analyze the fluxes in the central metabolism of the bacterium growing in a minimal medium. C-13-Metabolic flux analysis based on gas chromatography-mass spectrometry (GC-MS) confirmed the prevalent catabolic role of the Entner-Doudoroff pathway. Comparative nuclear magnetic resonance (NMR)-based isotopologue profiling of a mutant deficient in glycolysis gave evidence for a moderate flux via glycolysis in the wild-type. In addition to reconfirming the Entner-Doudoroff pathway as a catabolic main route, this approach affirmed a numerically minor but important flux via the pentose phosphate pathway.
Erscheinungsjahr
2014
Zeitschriftentitel
Molecular BioSystems
Band
10
Ausgabe
10
Seite(n)
2663-2676
ISSN
1742-206X
Page URI
https://pub.uni-bielefeld.de/record/2699819

Zitieren

Schatschneider S, Huber C, Neuweger H, et al. Metabolic flux pattern of glucose utilization by Xanthomonas campestris pv. campestris: prevalent role of the Entner-Doudoroff pathway and minor fluxes through the pentose phosphate pathway and glycolysis. Molecular BioSystems. 2014;10(10):2663-2676.
Schatschneider, S., Huber, C., Neuweger, H., Watt, T., Pühler, A., Eisenreich, W., Wittmann, C., et al. (2014). Metabolic flux pattern of glucose utilization by Xanthomonas campestris pv. campestris: prevalent role of the Entner-Doudoroff pathway and minor fluxes through the pentose phosphate pathway and glycolysis. Molecular BioSystems, 10(10), 2663-2676. doi:10.1039/c4mb00198b
Schatschneider, Sarah, Huber, Claudia, Neuweger, Heiko, Watt, Tony, Pühler, Alfred, Eisenreich, Wolfgang, Wittmann, Christoph, Niehaus, Karsten, and Vorhölter, Frank-Jörg. 2014. “Metabolic flux pattern of glucose utilization by Xanthomonas campestris pv. campestris: prevalent role of the Entner-Doudoroff pathway and minor fluxes through the pentose phosphate pathway and glycolysis”. Molecular BioSystems 10 (10): 2663-2676.
Schatschneider, S., Huber, C., Neuweger, H., Watt, T., Pühler, A., Eisenreich, W., Wittmann, C., Niehaus, K., and Vorhölter, F. - J. (2014). Metabolic flux pattern of glucose utilization by Xanthomonas campestris pv. campestris: prevalent role of the Entner-Doudoroff pathway and minor fluxes through the pentose phosphate pathway and glycolysis. Molecular BioSystems 10, 2663-2676.
Schatschneider, S., et al., 2014. Metabolic flux pattern of glucose utilization by Xanthomonas campestris pv. campestris: prevalent role of the Entner-Doudoroff pathway and minor fluxes through the pentose phosphate pathway and glycolysis. Molecular BioSystems, 10(10), p 2663-2676.
S. Schatschneider, et al., “Metabolic flux pattern of glucose utilization by Xanthomonas campestris pv. campestris: prevalent role of the Entner-Doudoroff pathway and minor fluxes through the pentose phosphate pathway and glycolysis”, Molecular BioSystems, vol. 10, 2014, pp. 2663-2676.
Schatschneider, S., Huber, C., Neuweger, H., Watt, T., Pühler, A., Eisenreich, W., Wittmann, C., Niehaus, K., Vorhölter, F.-J.: Metabolic flux pattern of glucose utilization by Xanthomonas campestris pv. campestris: prevalent role of the Entner-Doudoroff pathway and minor fluxes through the pentose phosphate pathway and glycolysis. Molecular BioSystems. 10, 2663-2676 (2014).
Schatschneider, Sarah, Huber, Claudia, Neuweger, Heiko, Watt, Tony, Pühler, Alfred, Eisenreich, Wolfgang, Wittmann, Christoph, Niehaus, Karsten, and Vorhölter, Frank-Jörg. “Metabolic flux pattern of glucose utilization by Xanthomonas campestris pv. campestris: prevalent role of the Entner-Doudoroff pathway and minor fluxes through the pentose phosphate pathway and glycolysis”. Molecular BioSystems 10.10 (2014): 2663-2676.

11 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

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