The cytosolic and extracellular proteomes of Actinoplanes sp. SE50/110 led to the identification of gene products involved in acarbose metabolism

Wendler S, Hürtgen D, Kalinowski J, Klein A, Niehaus K, Schulte F, Schwientek P, Wehlmann H, Wehmeier UF, Pühler A (2013)
Journal of Biotechnology 167(2): 178-189.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
The pseudotetrasaccharide acarbose is a medically relevant secondary metabolite produced by strains of the genera Actinoplanes and Streptomyces. In this study gene products involved in acarbose metabolism were identified by analyzing the cytosolic and extracellular proteome of Actinoplanes sp. SE50/110 cultures grown in a high-maltose minimal medium. The analysis by 2D protein gel electrophoresis of cytosolic proteins of Actinoplanes sp. SE50/110 resulted in 318 protein spots and 162 identified proteins. Nine of those were acarbose cluster proteins (Acb-proteins), namely AcbB, AcbD, AcbE, AcbK, AcbL, AcbN, AcbR, AcbV and AcbZ. The analysis of proteins in the extracellular space of Actinoplanes sp. SE50/110 cultures resulted in about 100 protein spots and 22 identified proteins. The identifications included the three acarbose gene cluster proteins AcbD, AcbE and AcbZ. After their identification, proteins were classified into functional groups. The dominant functional groups were the carbohydrate binding, carbohydrate cleavage and carbohydrate transport proteins. The other functional groups included protein cleavage, amino acid degradation, nucleic acid cleavage and a number of functionally uncharacterized proteins. In addition, signal peptide structures of extracellularly found proteins were analyzed. Of the 22 detected proteins 19 contained signal peptides, while 2 had N-terminal transmembrane helices explaining their localization. The only protein having neither of them was enolase. Under the conditions applied, the secretome of Actinoplanes sp. SE50/110 was dominated by seven proteins involved in carbohydrate metabolism (PulA, AcbE, AcbD, MalE, AglE, CbpA and Cgt). Of special interest were the identified extracellular pullulanase PulA and the two solute-binding proteins MalE and AglE. The identifications suggest that Actinoplanes sp. SE50/110 has two maltose/maltodextrin import systems. We postulate the identified MalEFG transport system of Actinoplanes sp. SE50/100 as the missing acarbose-metabolite importer and present a model of acarbose metabolism that is extended by the newly identified gene products.
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Journal of Biotechnology
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167
Ausgabe
2
Seite(n)
178-189
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Wendler S, Hürtgen D, Kalinowski J, et al. The cytosolic and extracellular proteomes of Actinoplanes sp. SE50/110 led to the identification of gene products involved in acarbose metabolism. Journal of Biotechnology. 2013;167(2):178-189.
Wendler, S., Hürtgen, D., Kalinowski, J., Klein, A., Niehaus, K., Schulte, F., Schwientek, P., et al. (2013). The cytosolic and extracellular proteomes of Actinoplanes sp. SE50/110 led to the identification of gene products involved in acarbose metabolism. Journal of Biotechnology, 167(2), 178-189. doi:10.1016/j.jbiotec.2012.08.011
Wendler, S., Hürtgen, D., Kalinowski, J., Klein, A., Niehaus, K., Schulte, F., Schwientek, P., Wehlmann, H., Wehmeier, U. F., and Pühler, A. (2013). The cytosolic and extracellular proteomes of Actinoplanes sp. SE50/110 led to the identification of gene products involved in acarbose metabolism. Journal of Biotechnology 167, 178-189.
Wendler, S., et al., 2013. The cytosolic and extracellular proteomes of Actinoplanes sp. SE50/110 led to the identification of gene products involved in acarbose metabolism. Journal of Biotechnology, 167(2), p 178-189.
S. Wendler, et al., “The cytosolic and extracellular proteomes of Actinoplanes sp. SE50/110 led to the identification of gene products involved in acarbose metabolism”, Journal of Biotechnology, vol. 167, 2013, pp. 178-189.
Wendler, S., Hürtgen, D., Kalinowski, J., Klein, A., Niehaus, K., Schulte, F., Schwientek, P., Wehlmann, H., Wehmeier, U.F., Pühler, A.: The cytosolic and extracellular proteomes of Actinoplanes sp. SE50/110 led to the identification of gene products involved in acarbose metabolism. Journal of Biotechnology. 167, 178-189 (2013).
Wendler, Sergej, Hürtgen, Daniel, Kalinowski, Jörn, Klein, Andreas, Niehaus, Karsten, Schulte, Fabian, Schwientek, Patrick, Wehlmann, Hermann, Wehmeier, Udo F., and Pühler, Alfred. “The cytosolic and extracellular proteomes of Actinoplanes sp. SE50/110 led to the identification of gene products involved in acarbose metabolism”. Journal of Biotechnology 167.2 (2013): 178-189.

12 Zitationen in Europe PMC

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Schwientek P, Neshat A, Kalinowski J, Klein A, Rückert C, Schneiker-Bekel S, Wendler S, Stoye J, Pühler A., J Biotechnol 190(), 2014
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4 References

Daten bereitgestellt von Europe PubMed Central.

Qupe--a Rich Internet Application to take a step forward in the analysis of mass spectrometry-based quantitative proteomics experiments.
Albaum SP, Neuweger H, Franzel B, Lange S, Mertens D, Trotschel C, Wolters D, Kalinowski J, Nattkemper TW, Goesmann A., Bioinformatics 25(23), 2009
PMID: 19808875
A proteomic view on genome-based signal peptide predictions.
Antelmann H, Tjalsma H, Voigt B, Ohlmeier S, Bron S, van Dijl JM, Hecker M., Genome Res. 11(9), 2001
PMID: 11544192

AUTHOR UNKNOWN, 0
[Pullulan, an extracellular glucan from Pullularia pullulans].
BENDER H, LEHMANN J, WALLENFELS K., Biochim. Biophys. Acta 36(), 1959
PMID: 13798777

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