Comparative analysis of the Hrp pathogenicity island of Rubus- and Spiraeoideae-infecting Erwinia amylovora strains identifies the IT region as a remnant of an integrative conjugative element

Mann, Rachel A; Blom, Jochen; Bühlmann, Andreas; Plummer, Kim M; Beer, Steven V; Luck, Joanne E; Goesmann, Alexander; Frey, Jürg E; Rodoni, Brendan C; Duffy, Brion; Smits, Theo H M

Comparative analysis of the Hrp pathogenicity island of Rubus- and Spiraeoideae-infecting Erwinia amylovora strains identifies the IT region as a remnant of an integrative conjugative element

Mann RA, Blom J, Bühlmann A, Plummer KM, Beer SV, Luck JE, Goesmann A, Frey JE, Rodoni BC, Duffy B, Smits THM (2012)
Gene 504(1): 6-12.

Zeitschriftenaufsatz | Veröffentlicht | Englisch

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DOI

https://doi.org/10.1016/j.gene.2012.05.002

Autor*in

Mann, Rachel A; Blom, Jochen^UniBi; Bühlmann, Andreas; Plummer, Kim M; Beer, Steven V; Luck, Joanne E; Goesmann, Alexander^UniBi ; Frey, Jürg E; Rodoni, Brendan C; Duffy, Brion; Smits, Theo H M

Einrichtung

Technische Fakultät > Computational Genomics
Centrum für Biotechnologie > Arbeitsgruppe A. Goesmann
Centrum für Biotechnologie > Technologieplattformen > Bioinformatics Resource Facility

Abstract / Bemerkung

The Hrp pathogenicity island (hrpPAI) of Erwinia amylovora not only encodes a type III secretion system (T3SS) and other genes required for pathogenesis on host plants, but also includes the so-called island transfer (IT) region, a region that originates from an integrative conjugative element (ICE). Comparative genomic analysis of the IT regions of two Spiraeoideae- and three Rubus-infecting strains revealed that the regions in Spiraeoideae-infecting strains were syntenic and highly conserved in length and genetic information, but that the IT regions of the Rubus-infecting strains varied in gene content and length, showing a mosaic structure. None of the ICEs in E. amylovora strains were complete, as conserved ICE genes and the left border were missing, probably due to reductive genome evolution. Comparison of the hrpPAI region of E. amylovora strains to syntenic regions from other Erwinia spp. indicates that the hrpPAI and the IT regions are the result of several insertion and deletion events that have occurred within the ICE. It also suggests that the T3SS was present in a common ancestor of the pathoadapted Erwinia spp. and that insertion and deletion events in the IT region occurred during speciation.

Erscheinungsjahr

2012

Zeitschriftentitel

Gene

Band

504

Ausgabe

Seite(n)

6-12

ISSN

0378-1119

Page URI

https://pub.uni-bielefeld.de/record/2500946

Zitieren

Mann RA, Blom J, Bühlmann A, et al. Comparative analysis of the Hrp pathogenicity island of Rubus- and Spiraeoideae-infecting Erwinia amylovora strains identifies the IT region as a remnant of an integrative conjugative element. Gene. 2012;504(1):6-12.

Mann, R. A., Blom, J., Bühlmann, A., Plummer, K. M., Beer, S. V., Luck, J. E., Goesmann, A., et al. (2012). Comparative analysis of the Hrp pathogenicity island of Rubus- and Spiraeoideae-infecting Erwinia amylovora strains identifies the IT region as a remnant of an integrative conjugative element. Gene, 504(1), 6-12. doi:10.1016/j.gene.2012.05.002

Mann, Rachel A, Blom, Jochen, Bühlmann, Andreas, Plummer, Kim M, Beer, Steven V, Luck, Joanne E, Goesmann, Alexander, et al. 2012. “Comparative analysis of the Hrp pathogenicity island of Rubus- and Spiraeoideae-infecting Erwinia amylovora strains identifies the IT region as a remnant of an integrative conjugative element”. Gene 504 (1): 6-12.

Mann, R. A., Blom, J., Bühlmann, A., Plummer, K. M., Beer, S. V., Luck, J. E., Goesmann, A., Frey, J. E., Rodoni, B. C., Duffy, B., et al. (2012). Comparative analysis of the Hrp pathogenicity island of Rubus- and Spiraeoideae-infecting Erwinia amylovora strains identifies the IT region as a remnant of an integrative conjugative element. Gene 504, 6-12.

Mann, R.A., et al., 2012. Comparative analysis of the Hrp pathogenicity island of Rubus- and Spiraeoideae-infecting Erwinia amylovora strains identifies the IT region as a remnant of an integrative conjugative element. Gene, 504(1), p 6-12.

R.A. Mann, et al., “Comparative analysis of the Hrp pathogenicity island of Rubus- and Spiraeoideae-infecting Erwinia amylovora strains identifies the IT region as a remnant of an integrative conjugative element”, Gene, vol. 504, 2012, pp. 6-12.

Mann, R.A., Blom, J., Bühlmann, A., Plummer, K.M., Beer, S.V., Luck, J.E., Goesmann, A., Frey, J.E., Rodoni, B.C., Duffy, B., Smits, T.H.M.: Comparative analysis of the Hrp pathogenicity island of Rubus- and Spiraeoideae-infecting Erwinia amylovora strains identifies the IT region as a remnant of an integrative conjugative element. Gene. 504, 6-12 (2012).

Mann, Rachel A, Blom, Jochen, Bühlmann, Andreas, Plummer, Kim M, Beer, Steven V, Luck, Joanne E, Goesmann, Alexander, Frey, Jürg E, Rodoni, Brendan C, Duffy, Brion, and Smits, Theo H M. “Comparative analysis of the Hrp pathogenicity island of Rubus- and Spiraeoideae-infecting Erwinia amylovora strains identifies the IT region as a remnant of an integrative conjugative element”. Gene 504.1 (2012): 6-12.

Daten bereitgestellt von European Bioinformatics Institute (EBI)

12 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

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Bühlmann A, Dreo T, Rezzonico F, Pothier JF, Smits TH, Ravnikar M, Frey JE, Duffy B., Environ Microbiol 16(7), 2014
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Thapa SP, Park DH, Kim WS, Choi BS, Lim JS, Choi IY, Hur JH, Lim CK., Genome 56(2), 2013
PMID: 23517317

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Bühlmann A, Pothier JF, Rezzonico F, Smits TH, Andreou M, Boonham N, Duffy B, Frey JE., J Microbiol Methods 92(3), 2013
PMID: 23275135

Comparative genomics of 12 strains of Erwinia amylovora identifies a pan-genome with a large conserved core.
Mann RA, Smits TH, Bühlmann A, Blom J, Goesmann A, Frey JE, Plummer KM, Beer SV, Luck J, Duffy B, Rodoni B., PLoS One 8(2), 2013
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Smits TH, Rezzonico F, López MM, Blom J, Goesmann A, Frey JE, Duffy B., Syst Appl Microbiol 36(7), 2013
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Malnoy M, Martens S, Norelli JL, Barny MA, Sundin GW, Smits TH, Duffy B., Annu Rev Phytopathol 50(), 2012
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