Genome-enabled determination of amino acid biosynthesis in Xanthomonas campestris pv. campestris and identification of biosynthetic pathways for alanine, glycine, and isoleucine by 13C-isotopologue profiling

Schatschneider S, Vorhölter F-J, Rückert C, Becker A, Eisenreich W, Pühler A, Niehaus K (2011)
Molecular Genetics and Genomics 286(3-4): 247-259.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
Abstract / Bemerkung
To elucidate the biosynthetic pathways for all proteinogenic amino acids in Xanthomonas campestris pv. campestris, this study combines results obtained by in silico genome analysis and by 13C-NMR-based isotopologue profiling to provide a panoramic view on a substantial section of bacterial metabolism. Initially, biosynthesis pathways were reconstructed from an improved annotation of the complete genome of X. campestris pv. campestris B100. This metabolic reconstruction resulted in the unequivocal identification of biosynthesis routes for 17 amino acids in total: arginine, asparagine, aspartate, cysteine, glutamate, glutamine, histidine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine. Ambiguous pathways were reconstructed from the genome data for alanine, glycine, and isoleucine biosynthesis. 13C-NMR analyses supported the identification of the metabolically active pathways. The biosynthetic routes for these amino acids were derived from the precursor molecules pyruvate, serine, and pyruvate, respectively. By combining genome analysis and isotopologue profiling, a comprehensive set of biosynthetic pathways covering all proteinogenic amino acids was unraveled for this plant pathogenic bacterium, which plays an important role in biotechnology as a producer of the exopolysaccharide xanthan. The data obtained lay ground for subsequent functional analyses in post-genomics and biotechnology, while the innovative combination of in silico and wet lab technology described here is promising as a general approach to elucidate metabolic pathways.
Erscheinungsjahr
Zeitschriftentitel
Molecular Genetics and Genomics
Band
286
Ausgabe
3-4
Seite(n)
247-259
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eISSN
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Schatschneider S, Vorhölter F-J, Rückert C, et al. Genome-enabled determination of amino acid biosynthesis in Xanthomonas campestris pv. campestris and identification of biosynthetic pathways for alanine, glycine, and isoleucine by 13C-isotopologue profiling. Molecular Genetics and Genomics. 2011;286(3-4):247-259.
Schatschneider, S., Vorhölter, F. - J., Rückert, C., Becker, A., Eisenreich, W., Pühler, A., & Niehaus, K. (2011). Genome-enabled determination of amino acid biosynthesis in Xanthomonas campestris pv. campestris and identification of biosynthetic pathways for alanine, glycine, and isoleucine by 13C-isotopologue profiling. Molecular Genetics and Genomics, 286(3-4), 247-259. doi:10.1007/s00438-011-0639-7
Schatschneider, S., Vorhölter, F. - J., Rückert, C., Becker, A., Eisenreich, W., Pühler, A., and Niehaus, K. (2011). Genome-enabled determination of amino acid biosynthesis in Xanthomonas campestris pv. campestris and identification of biosynthetic pathways for alanine, glycine, and isoleucine by 13C-isotopologue profiling. Molecular Genetics and Genomics 286, 247-259.
Schatschneider, S., et al., 2011. Genome-enabled determination of amino acid biosynthesis in Xanthomonas campestris pv. campestris and identification of biosynthetic pathways for alanine, glycine, and isoleucine by 13C-isotopologue profiling. Molecular Genetics and Genomics, 286(3-4), p 247-259.
S. Schatschneider, et al., “Genome-enabled determination of amino acid biosynthesis in Xanthomonas campestris pv. campestris and identification of biosynthetic pathways for alanine, glycine, and isoleucine by 13C-isotopologue profiling”, Molecular Genetics and Genomics, vol. 286, 2011, pp. 247-259.
Schatschneider, S., Vorhölter, F.-J., Rückert, C., Becker, A., Eisenreich, W., Pühler, A., Niehaus, K.: Genome-enabled determination of amino acid biosynthesis in Xanthomonas campestris pv. campestris and identification of biosynthetic pathways for alanine, glycine, and isoleucine by 13C-isotopologue profiling. Molecular Genetics and Genomics. 286, 247-259 (2011).
Schatschneider, Sarah, Vorhölter, Frank-Jörg, Rückert, Christian, Becker, Anke, Eisenreich, Wolfgang, Pühler, Alfred, and Niehaus, Karsten. “Genome-enabled determination of amino acid biosynthesis in Xanthomonas campestris pv. campestris and identification of biosynthetic pathways for alanine, glycine, and isoleucine by 13C-isotopologue profiling”. Molecular Genetics and Genomics 286.3-4 (2011): 247-259.

11 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Refined annotation of the complete genome of the phytopathogenic and xanthan producing Xanthomonas campestris pv. campestris strain B100 based on RNA sequence data.
Alkhateeb RS, Rückert C, Rupp O, Pucker B, Hublik G, Wibberg D, Niehaus K, Pühler A, Vorhölter FJ., J Biotechnol 253(), 2017
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Systems and synthetic biology perspective of the versatile plant-pathogenic and polysaccharide-producing bacterium Xanthomonas campestris.
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Unconventional membrane lipid biosynthesis in Xanthomonas campestris.
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Establishment, in silico analysis, and experimental verification of a large-scale metabolic network of the xanthan producing Xanthomonas campestris pv. campestris strain B100.
Schatschneider S, Persicke M, Watt SA, Hublik G, Pühler A, Niehaus K, Vorhölter FJ., J Biotechnol 167(2), 2013
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The noncanonical type III secretion system of Xanthomonas translucens pv. graminis is essential for forage grass infection.
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